scholarly journals Protein, Oil, and Isoflavone Contents in Lipoxygenase- and Kunitz Trypsin Inhibitor-Deficient Soybean Seeds

2007 ◽  
Vol 66 (7-8) ◽  
pp. 521-527 ◽  
Author(s):  
Maria Isaura P. Oliveira ◽  
Nilton D. Piovesan ◽  
Ines C. José ◽  
Everaldo G. Barros ◽  
Maurilio A. Moreira ◽  
...  
Keyword(s):  
Trypsin Inhibitor ◽  
Soybean Seeds ◽  
Kunitz Trypsin Inhibitor

An Efficient HPLC Approach to Quantify Kunitz Trypsin Inhibitor in Soybean Seeds

Crop Science ◽  
2018 ◽  
Vol 58 (4) ◽  
pp. 1616-1623 ◽  
Author(s):  
M. Luciana Rosso ◽  
Chao Shang ◽  
Edgar Correa ◽  
Bo Zhang
Keyword(s):  
Trypsin Inhibitor ◽  
Soybean Seeds ◽  
Kunitz Trypsin Inhibitor

scholarly journals Development of Breeder-Friendly KASP Markers for Low Concentration of Kunitz Trypsin Inhibitor in Soybean Seeds

2021 ◽  
Vol 22 (5) ◽  
pp. 2675
Author(s):  
M. Luciana Rosso ◽  
Chao Shang ◽  
Qijian Song ◽  
Diana Escamilla ◽  
Jay Gillenwater ◽  
...  
Keyword(s):  
Trypsin Inhibitor ◽  
Protein Digestibility ◽  
Population Based ◽  
Hplc Method ◽  
Snp Markers ◽  
Soybean Seeds ◽  
Kunitz Trypsin Inhibitor ◽  
Breeding Lines ◽  
Specific Pcr ◽  
Kasp Assay

Trypsin inhibitors (TI), a common anti-nutritional factor in soybean, prevent animals’ protein digestibility reducing animal growth performance. No commercial soybean cultivars with low or null concentration of TI are available. The availability of a high throughput genotyping assay will be beneficial to incorporate the low TI trait into elite breeding lines. The aim of this study is to develop and validate a breeder friendly Kompetitive Allele Specific PCR (KASP) assay linked to low Kunitz trypsin inhibitor (KTI) in soybean seeds. A total of 200 F3:5 lines derived from PI 547656 (low KTI) X Glenn (normal KTI) were genotyped using the BARCSoySNP6K_v2 Beadchip. F3:4 and F3:5 lines were grown in Blacksburg and Orange, Virginia in three years, respectively, and were measured for KTI content using a quantitative HPLC method. We identified three SNP markers tightly linked to the major QTL associated to low KTI in the mapping population. Based on these SNPs, we developed and validated the KASP assays in a set of 93 diverse germplasm accessions. The marker Gm08_44814503 has 86% selection efficiency for the accessions with low KTI and could be used in marker assisted breeding to facilitate the incorporation of low KTI content in soybean seeds.

scholarly journals Kunitz trypsin Inhibitor and phytic acid levels in conventional and genetically modified soybean seeds from Londrina and Ponta Grossa, South Brazil

2014 ◽  
Vol 36 (4) ◽  
pp. 727-731 ◽  
Author(s):  
Olívio F. Galão ◽  
Mercedes C. Carrão-Panizzi ◽  
José Marcos G. Mandarino ◽  
Rodrigo Santos Leite ◽  
Thiago Claus ◽  
...  
Keyword(s):  
Phytic Acid ◽  
Trypsin Inhibitor ◽  
Genetically Modified ◽  
Soybean Seeds ◽  
Kunitz Trypsin Inhibitor ◽  
Genetically Modified Soybean ◽  
South Brazil

Assisted Selection by Specific DNA Markers for Genetic Elimination of the Kunitz Trypsin Inhibitor and Lectin in Soybean Seeds

Euphytica ◽  
2006 ◽  
Vol 149 (1-2) ◽  
pp. 221-226 ◽  
Author(s):  
Rita Maria Alves de Moraes ◽  
Tais Cristina Bastos Soares ◽  
Lucinete Regina Colombo ◽  
Maria Fernanda Spegiorin Salla ◽  
Josie Gomes de Almeida Barros ◽  
...  
Keyword(s):  
Trypsin Inhibitor ◽  
Dna Markers ◽  
Soybean Seeds ◽  
Kunitz Trypsin Inhibitor
Sign in / Sign up

Export Citation Format

Share Document