scholarly journals Development of Breeder-Friendly KASP Markers for Low Concentration of Kunitz Trypsin Inhibitor in Soybean Seeds

2021 ◽  
Vol 22 (5) ◽  
pp. 2675
Author(s):  
M. Luciana Rosso ◽  
Chao Shang ◽  
Qijian Song ◽  
Diana Escamilla ◽  
Jay Gillenwater ◽  
...  
Keyword(s):  
Trypsin Inhibitor ◽  
Protein Digestibility ◽  
Population Based ◽  
Hplc Method ◽  
Snp Markers ◽  
Soybean Seeds ◽  
Kunitz Trypsin Inhibitor ◽  
Breeding Lines ◽  
Specific Pcr ◽  
Kasp Assay

Trypsin inhibitors (TI), a common anti-nutritional factor in soybean, prevent animals’ protein digestibility reducing animal growth performance. No commercial soybean cultivars with low or null concentration of TI are available. The availability of a high throughput genotyping assay will be beneficial to incorporate the low TI trait into elite breeding lines. The aim of this study is to develop and validate a breeder friendly Kompetitive Allele Specific PCR (KASP) assay linked to low Kunitz trypsin inhibitor (KTI) in soybean seeds. A total of 200 F3:5 lines derived from PI 547656 (low KTI) X Glenn (normal KTI) were genotyped using the BARCSoySNP6K_v2 Beadchip. F3:4 and F3:5 lines were grown in Blacksburg and Orange, Virginia in three years, respectively, and were measured for KTI content using a quantitative HPLC method. We identified three SNP markers tightly linked to the major QTL associated to low KTI in the mapping population. Based on these SNPs, we developed and validated the KASP assays in a set of 93 diverse germplasm accessions. The marker Gm08_44814503 has 86% selection efficiency for the accessions with low KTI and could be used in marker assisted breeding to facilitate the incorporation of low KTI content in soybean seeds.

An Efficient HPLC Approach to Quantify Kunitz Trypsin Inhibitor in Soybean Seeds

Crop Science ◽  
2018 ◽  
Vol 58 (4) ◽  
pp. 1616-1623 ◽  
Author(s):  
M. Luciana Rosso ◽  
Chao Shang ◽  
Edgar Correa ◽  
Bo Zhang
Keyword(s):  
Trypsin Inhibitor ◽  
Soybean Seeds ◽  
Kunitz Trypsin Inhibitor

scholarly journals Protein, Oil, and Isoflavone Contents in Lipoxygenase- and Kunitz Trypsin Inhibitor-Deficient Soybean Seeds

2007 ◽  
Vol 66 (7-8) ◽  
pp. 521-527 ◽  
Author(s):  
Maria Isaura P. Oliveira ◽  
Nilton D. Piovesan ◽  
Ines C. José ◽  
Everaldo G. Barros ◽  
Maurilio A. Moreira ◽  
...  
Keyword(s):  
Trypsin Inhibitor ◽  
Soybean Seeds ◽  
Kunitz Trypsin Inhibitor

A linked SNP marker to genotype Fr-B2 in wheat

2018 ◽  
Vol 69 (9) ◽  
pp. 859
Author(s):  
H. A. Eagles ◽  
J. Hyles ◽  
Jayne Wilson ◽  
Karen Cane ◽  
K. L. Forrest ◽  
...  
Keyword(s):  
Triticum Aestivum L ◽  
Frost Tolerance ◽  
Large Deletion ◽  
Wheat Breeding ◽  
Snp Markers ◽  
Breeding Lines ◽  
Specific Pcr ◽  
Chromosome 5B ◽  
Allele Specific ◽  
Specific Pcr Assay

Fr-B2 is a complex locus on chromosome 5B that affects frost tolerance, days to heading, grain yield and probably other traits of commercial importance in wheat (Triticum aestivum L.). It interacts epistatically with other major genes, especially VRN1. There are two known alleles of Fr-B2: an intact, wild-type allele, and an allele with a large deletion. Published methods for identifying these alleles are slow and expensive, making the development of a high-throughput, co-dominant SNP (single-nucleotide polymorphism) marker highly desirable, especially for commercial wheat breeding. A diverse panel of cultivars and breeding lines was characterised for SNPs and alleles of Fr-B2. Four SNP markers co-segregated as a haplotype block with Fr-B2 across unrelated cultivars and related backcrosses differing for alleles of Fr-B2. A robust KASP (Kompetitive allele-specific PCR) assay was developed for one of the SNPs, KASP_IWB26333, which should facilitate the inclusion of Fr-B2 on genotyping platforms for breeding and research.

scholarly journals Kunitz trypsin Inhibitor and phytic acid levels in conventional and genetically modified soybean seeds from Londrina and Ponta Grossa, South Brazil

2014 ◽  
Vol 36 (4) ◽  
pp. 727-731 ◽  
Author(s):  
Olívio F. Galão ◽  
Mercedes C. Carrão-Panizzi ◽  
José Marcos G. Mandarino ◽  
Rodrigo Santos Leite ◽  
Thiago Claus ◽  
...  
Keyword(s):  
Phytic Acid ◽  
Trypsin Inhibitor ◽  
Genetically Modified ◽  
Soybean Seeds ◽  
Kunitz Trypsin Inhibitor ◽  
Genetically Modified Soybean ◽  
South Brazil

Assisted Selection by Specific DNA Markers for Genetic Elimination of the Kunitz Trypsin Inhibitor and Lectin in Soybean Seeds

Euphytica ◽  
2006 ◽  
Vol 149 (1-2) ◽  
pp. 221-226 ◽  
Author(s):  
Rita Maria Alves de Moraes ◽  
Tais Cristina Bastos Soares ◽  
Lucinete Regina Colombo ◽  
Maria Fernanda Spegiorin Salla ◽  
Josie Gomes de Almeida Barros ◽  
...  
Keyword(s):  
Trypsin Inhibitor ◽  
Dna Markers ◽  
Soybean Seeds ◽  
Kunitz Trypsin Inhibitor

scholarly journals New Transcriptome-Based SNP Markers for Noug (Guizotia abyssinica) and Their Conversion to KASP Markers for Population Genetics Analyses

Genes ◽  
2020 ◽  
Vol 11 (11) ◽  
pp. 1373
Author(s):  
Sewalem Tsehay ◽  
Rodomiro Ortiz ◽  
Eva Johansson ◽  
Endashaw Bekele ◽  
Kassahun Tesfaye ◽  
...  
Keyword(s):  
Population Genetics ◽  
Snp Markers ◽  
Oilseed Crop ◽  
Nucleotide Polymorphisms ◽  
Guizotia Abyssinica ◽  
Genomic Resources ◽  
Genetics Research ◽  
Specific Pcr ◽  
Kasp Assay ◽  
Hardy Weinberg Equilibrium

The development and use of genomic resources are essential for understanding the population genetics of crops for their efficient conservation and enhancement. Noug (Guizotia abyssinica) is an economically important oilseed crop in Ethiopia and India. The present study sought to develop new DNA markers for this crop. Transcriptome sequencing was conducted on two genotypes and 628 transcript sequences containing 959 single nucleotide polymorphisms (SNPs) were developed. A competitive allele-specific PCR (KASP) assay was developed for the SNPs and used for genotyping of 24 accessions. A total of 554 loci were successfully genotyped across the accessions, and 202 polymorphic loci were used for population genetics analyses. Polymorphism information content (PIC) of the loci varied from 0.01 to 0.37 with a mean of 0.24, and about 49% of the loci showed significant deviation from the Hardy-Weinberg equilibrium. The mean expected heterozygosity was 0.27 suggesting moderately high genetic variation within accessions. Low but significant differentiation existed among accessions (FST = 0.045, p < 0.0001). Landrace populations from isolated areas may have useful mutations and should be conserved and used in breeding this crop. The genomic resources developed in this study were shown to be useful for population genetics research and can also be used in, e.g., association genetics.

Marker-assisted stacking of null Kunitz trypsin inhibitor and off-flavour generating lipoxygenase-2 in soybean

2021 ◽  
pp. 1-9
Author(s):  
V. Kumar ◽  
A. Rani ◽  
A. K. Anshu ◽  
T. Tayalkar
Keyword(s):  
Ssr Markers ◽  
Trypsin Inhibitor ◽  
Null Allele ◽  
Protein Digestibility ◽  
Null Alleles ◽  
Human Consumption ◽  
Recurrent Parent ◽  
Kunitz Trypsin Inhibitor ◽  
Soybean Genotypes ◽  
Parental Combination

Abstract Soybean varieties genetically free from Kunitz trypsin inhibitor (KTI) and lipoxygenase-2 (Lox2) are desirable to increase human consumption, as the former is an antinutritional factor that affects protein digestibility while the latter is a principal contributor to off-flavour. In the present investigation, soybean genotypes free from both these undesirable components were developed by introgression of null allele of Lox2 from NRC109 (lox2lox2) into two KTI-free soybean genotypes derived from genotypes JS97-52 and NRC7. Foreground selection of plants in F1, F2, BC1F1, BC1F2, BC2F1 and BC2F2 generations developed from two cross combinations i.e. NRC7-derived KTI-free genotype (N7KTIF)×NRC109 (parental combination 1) and JS97-52 derived KTI free genotype (JKTIF)×NRC109 (parental combination 2) was performed using null allele specific markers and tightly linked simple sequence repeat (SSR) markers for both KTI and Lox2 genes for the identification of homozygous recessive (titilox2lox2) plants. Background selection was performed using 239 and 241 polymorphic SSR markers across the genome. This resulted in the development of 9 and 13 soybean lines stacked for null alleles of both KTI and Lox2 (titilox2lox2) exhibiting recurrent parent genome content more than 97 and 96%, respectively. Days-to-flowering, days-to-maturity, 100-seed weight and yield per plant of the stacked lines developed from both the parental combinations were at par with the respective recurrent parents.

Sign in / Sign up

Export Citation Format

Share Document