A New Fiber-Optic-Based Multifrequency Phase-Modulation Fluorometer

1988 ◽  
Vol 42 (8) ◽  
pp. 1531-1537 ◽  
Author(s):  
Frank V. Bright
Keyword(s):  
Fluorescence Lifetime ◽  
Phase Modulation ◽  
Fiber Optic ◽  
Fluorescence Decay ◽  
Fiber Optic Probe ◽  
Decay Kinetics ◽  
Spectral Coverage ◽  
New Instrument ◽  
Fluorescence Decay Kinetics ◽  
Optic Probe

A new fiber-optic-based fluorescence lifetime instrument is described which is useful for the study of complex fluorescence decay kinetics. The new instrument consists of a multifrequency phase fluorometer coupled to a fiber-optic probe. To demonstrate the power of the new instrument, we show results for the unequivocal resolution of single, double, and triple exponential decays of fluorescence. In addition, spectral coverage in the ultraviolet and visible regions is possible, and lifetimes from approximately 200 ns to 150 ps are easily determined for samples as dilute as 0.1 μM.

scholarly journals Analysis of Fluorescence Decay Kinetics of Indocyanine Green Monomers and Aggregates in Brain Tumor Model In Vivo

Nanomaterials ◽  
2021 ◽  
Vol 11 (12) ◽  
pp. 3185
Author(s):  
Dina Farrakhova ◽  
Igor Romanishkin ◽  
Yuliya Maklygina ◽  
Lina Bezdetnaya ◽  
Victor Loschenov
Keyword(s):  
Brain Tumor ◽  
Indocyanine Green ◽  
Fluorescence Lifetime ◽  
Tumor Model ◽  
Fluorescence Decay ◽  
Decay Kinetics ◽  
Lifetime Analysis ◽  
Fluorescence Decay Kinetics ◽  
Brain Tumor Model

Spectroscopic approach with fluorescence time resolution allows one to determine the state of a brain tumor and its microenvironment via changes in the fluorescent dye’s fluorescence lifetime. Indocyanine green (ICG) is an acknowledged infra-red fluorescent dye that self-assembles into stable aggregate forms (ICG NPs). ICG NPs aggregates have a tendency to accumulate in the tumor with a maximum accumulation at 24 h after systemic administration, enabling extended intraoperative diagnostic. Fluorescence lifetime analysis of ICG and ICG NPs demonstrates different values for ICG monomers and H-aggregates, indicating promising suitability for fluorescent diagnostics of brain tumors due to their affinity to tumor cells and stability in biological tissue.

scholarly journals Fluorescence lifetime spectroscopy of tissue autofluorescence in normal and diseased colon measured ex vivo using a fiber-optic probe

2014 ◽  
Vol 5 (2) ◽  
pp. 515 ◽  
Author(s):  
Sergio Coda ◽  
Alex J. Thompson ◽  
Gordon T. Kennedy ◽  
Kim L. Roche ◽  
Lakshmana Ayaru ◽  
...  
Keyword(s):  
Fluorescence Lifetime ◽  
Fiber Optic ◽  
Ex Vivo ◽  
Fiber Optic Probe ◽  
Optic Probe

Synthesis, performance and growth mechanism of silver nanoparticle coated SERS fiber probe

2019 ◽  
Vol 107 (3) ◽  
pp. 305
Author(s):  
Mengmei Geng ◽  
Yuting Long ◽  
Tongqing Liu ◽  
Zijuan Du ◽  
Hong Li ◽  
...  
Keyword(s):  
Silver Nanoparticles ◽  
Absorption Spectrum ◽  
Reaction Time ◽  
Growth Mechanism ◽  
Fiber Optic ◽  
Fiber Optic Probe ◽  
Visible Absorption ◽  
Fiber Probe ◽  
Surface Enhanced Raman ◽  
Optic Probe

Surface-enhanced Raman Scattering (SERS) fiber probe provides abundant interaction area between light and materials, permits detection within limited space and is especially useful for remote or in situ detection. A silver decorated SERS fiber optic probe was prepared by hydrothermal method. This method manages to accomplish the growth of silver nanoparticles and its adherence on fiber optic tip within one step, simplifying the synthetic procedure. The effects of reaction time on phase composition, surface plasmon resonance property and morphology were investigated by X-ray diffraction analysis (XRD), ultraviolet-visible absorption spectrum (UV-VIS absorption spectrum) and scanning electron microscope (SEM). The results showed that when reaction time is prolonged from 4–8 hours at 180 °C, crystals size and size distribution of silver nanoparticles increase. Furthermore, the morphology, crystal size and distribution density of silver nanoparticles evolve along with reaction time. A growth mechanism based on two factors, equilibrium between nucleation and growth, and the existence of PVP, is hypothesized. The SERS fiber probe can detect rhodamin 6G (R6G) at the concentration of 10−6 M. This SERS fiber probe exhibits promising potential in organic dye and pesticide residue detection.

Evaluation of Dry-Wet Transition in PEFC Under Load Changes Based on Laser Spectroscopy Using Fiber-Optic Probe

2019 ◽  
Vol 92 (8) ◽  
pp. 119-124
Author(s):  
Kosuke Nishida ◽  
Ryoga Nakauchi ◽  
Yuma Tabata ◽  
Toyofumi Umekawa ◽  
Masahiro Kawasaki
Keyword(s):  
Laser Spectroscopy ◽  
Fiber Optic ◽  
Fiber Optic Probe ◽  
Optic Probe

scholarly journals Longitudinal Raman Spectroscopic Observation of Skin Biochemical Changes due to Chemotherapeutic Treatment for Breast Cancer in Small Animal Model

2017 ◽  
Vol 2017 ◽  
pp. 1-9
Author(s):  
Myeongsu Seong ◽  
NoSoung Myoung ◽  
Songhyun Lee ◽  
Hyeryun Jeong ◽  
Sang-Youp Yim ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Animal Model ◽  
Raman Spectra ◽  
Fiber Optic ◽  
Animal Experiments ◽  
Small Animal ◽  
Fiber Optic Probe ◽  
Small Animal Model ◽  
Biochemical Compositions ◽  
Optic Probe

The cancer field effect (CFE) has been highlighted as one of indirect indications for tissue variations that are insensitive to conventional diagnostic techniques. In this research, we had a hypothesis that chemotherapy for breast cancer would affect skin biochemical compositions that would be reflected by Raman spectral changes. We used a fiber-optic probe-based Raman spectroscopy to perform preliminary animal experiments to validate the hypothesis. Firstly, we verified the probing depth of the fiber-optic probe (~800 μm) using a simple intravenous fat emulsion-filled phantom having a silicon wafer at the bottom inside a cuvette. Then, we obtained Raman spectra during breast cancer treatment by chemotherapy from a small animal model in longitudinal manner. Our results showed that the treatment causes variations of biochemical compositions in the skin. For further validation, the Raman spectra will have to be collected from more populations and spectra will need to be compared with immunohistochemistry of the breast tissue.

scholarly journals Autofluorescence and diffuse reflectance spectroscopy of oral epithelial tissue using a depth-sensitive fiber-optic probe

2008 ◽  
Vol 47 (6) ◽  
pp. 825 ◽  
Author(s):  
Richard A. Schwarz ◽  
Wen Gao ◽  
Dania Daye ◽  
Michelle D. Williams ◽  
Rebecca Richards-Kortum ◽  
...  
Keyword(s):  
Diffuse Reflectance ◽  
Fiber Optic ◽  
Diffuse Reflectance Spectroscopy ◽  
Reflectance Spectroscopy ◽  
Epithelial Tissue ◽  
Fiber Optic Probe ◽  
Optic Probe ◽  
Oral Epithelial
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