Reversing the Effects of Formalin Fixation with Citraconic Anhydride and Heat: A Universal Antigen Retrieval Method

2005 ◽  
Vol 53 (1) ◽  
pp. 3-11 ◽  
Author(s):  
S. Namimatsu
Keyword(s):  
Formalin Fixation ◽  
Antigen Retrieval ◽  
Retrieval Method ◽  
Citraconic Anhydride

scholarly journals A Molecular Mechanism of Formalin Fixation and Antigen Retrieval

2004 ◽  
Vol 121 (2) ◽  
pp. 190-199 ◽  
Author(s):  
Seshi R. Sompuram ◽  
Kodela Vani ◽  
Elizabeth Messana ◽  
Steven A. Bogen
Keyword(s):  
Molecular Mechanism ◽  
Formalin Fixation ◽  
Antigen Retrieval

scholarly journals A simple and effective heat induced antigen retrieval method

MethodsX ◽  
2016 ◽  
Vol 3 ◽  
pp. 315-319 ◽  
Author(s):  
Vinod K.R. ◽  
Denny Jones ◽  
Venkatesha Udupa
Keyword(s):  
Antigen Retrieval ◽  
Retrieval Method ◽  
Effective Heat

A simple and sensitive antigen retrieval method for free-floating and slide-mounted tissue sections

1999 ◽  
Vol 93 (2) ◽  
pp. 149-162 ◽  
Author(s):  
Yun Jiao ◽  
Zhiqiang Sun ◽  
Teffy Lee ◽  
Francesca R Fusco ◽  
Toya D Kimble ◽  
...  
Keyword(s):  
Antigen Retrieval ◽  
Retrieval Method ◽  
Tissue Sections

scholarly journals 2. Histocytochemistry Series A New Antigen Retrieval Method (6)

2010 ◽  
Vol 6 (4) ◽  
pp. 178-184
Author(s):  
Shigeki Namimatsu ◽  
Yuichi Sugisaki ◽  
Shinichi Tsuchiya
Keyword(s):  
Antigen Retrieval ◽  
Retrieval Method

Standardization of Routine Immunohistochemistry (IHC) by Antigen Retrieval (AR): is it Possible?

1997 ◽  
Vol 3 (S2) ◽  
pp. 161-162
Author(s):  
S.-R. Shi ◽  
B. Chaiwun ◽  
R.J. Cote ◽  
L. Young ◽  
T. Chen ◽  
...  
Keyword(s):  
World Wide ◽  
Critical Issue ◽  
Formalin Fixation ◽  
Antigen Retrieval ◽  
Variable Intensity ◽  
Diagnostic Pathology ◽  
Significant Challenge ◽  
Optimal Protocol ◽  
The World ◽  
Adverse Influence

The standardization of IHC has been a critical issue since 1977. The most significant challenge for the standardization of routine IHC may be the adverse influence of formalin, which is in part due to variable periods of formalin-fixation ranging from 12 hours to days or weeks, resulting in variable intensity of immunostaining for formalin sensitive antigens. The world-wide use of the AR technique for IHC in both clinical and research histopathology has demonstrated that the AR is a breakthrough in pathology. Based on numerous studies of AR-IHC with excellent results for a variety of interesting markers used in diagnostic pathology, the possibility has been raised that AR technique may be useful in the standardization of IHC. The purpose of this study is to demonstrate the possibility of standardization of IHC based on the ‘test battery‘ approach for establishing an optimal protocol of AR in order to achieve a state of ‘maximal retrieval’, showing a equal comparability of AR-IHC staining of archival paraffin-embedded tissues under varying periods of formalin-fixation.

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