Optimal verification of the Bell state and Greenberger–Horne–Zeilinger states in untrusted quantum networks

Bipartite and multipartite entangled states are basic ingredients for constructing quantum networks and their accurate verification is crucial to the functioning of the networks, especially for untrusted networks. Here we propose a simple approach for verifying the Bell state in an untrusted network in which one party is not honest. Only local projective measurements are required for the honest party. It turns out each verification protocol is tied to a probability distribution on the Bloch sphere and its performance has an intuitive geometric meaning. This geometric picture enables us to construct the optimal and simplest verification protocols, which are also very useful to detecting entanglement in the untrusted network. Moreover, we show that our verification protocols can achieve almost the same sample efficiencies as protocols tailored to standard quantum state verification. Furthermore, we establish an intimate connection between the verification of Greenberger–Horne–Zeilinger states and the verification of the Bell state. By virtue of this connection we construct the optimal protocol for verifying Greenberger–Horne–Zeilinger states and for detecting genuine multipartite entanglement.

Comparison of methods to extract PCR-amplifiable DNA from fruit, herbal and black teas

The success of polymerase chain reaction (PCR) assay depends on template deoxyribonucleic acid (DNA) being sufficient with respect to both quantity and quality. Some biological materials contain compounds which inhibit the functioning of DNA polymerase and thus need to be removed as part of the DNA extraction procedure. The aim of the present experiments was to optimise the process of DNA isolation from various types of black, fruit and herbal teas. A comparison was made between two cetyltrimethylammonium bromide (CTAB)-based protocols and two commercially available DNA purification kits. The yield and integrity of the extracted DNA were monitored both spectrophotometrically and using agarose gel electrophoresis. The presence/absence of inhibitors in the DNA preparations was checked by running quantitative real-time PCRs. The optimal protocol was deemed to be the CTAB method described in ISO 21571:2005, so this method is recommended for the routine sample analysis of tea products.

Engrafting Horse Immune Cells into Mouse Hosts for the Study of the Acute Equine Immune Responses

Immunological studies in the horse are frequently hampered by lack of environmental control, complicated study design, and ethical concerns when performing high risk studies. The purpose of the current study was to investigate the utility of a xenograft model for studying acute equine immune responses. Immunocompromised non obese diabetic (NOD). sudden combined immunodeficiency (scid).gamma−/− (NSG) mice were engrafted with either equine peripheral blood lymphocytes (PBLs) or equine bone marrow to determine an optimal protocol for equine lymphocyte engraftment. We found that both PBL and bone marrow grafts populated the host mice successfully. Bone marrow transplants were technically more challenging and required further processing to retard graft versus host disease. Graft vs host disease was apparent at 28 days post-PBL transfer and 56 days post-bone marrow transfer. The results of these studies support the use of mouse hosts to study acute equine immune responses and that different engraftment techniques can be used depending on the experimental design.

Effectiveness of modified downregulation for women with moderate and severe adenomyosis of the uterus prior to frozen thawed embryo transfer (MODA) study protocol: a pragmatic randomised-controlled trial

IntroductionAdenomyosis can adversely reduce chances of pregnancy in couples undergoing assisted conception. We aim to evaluate the effect of two different downregulation protocols on the reproductive outcomes in women with moderate and severe adenomyosis undergoing frozen-thawed embryo transfer (FTET).Methods and analysisWe will conduct a two-armed pragmatic randomised clinical trial comparing modified downregulation with gonadotrophin-releasing hormone (GnRH) analogue for 6 weeks to standard downregulation with GnRH analogue for 1 week prior to FTET. Our primary outcome is clinical pregnancy, defined as a viable intrauterine pregnancy confirmed by ultrasound at greater than 6 weeks gestation, with other secondary reproductive, neonatal and safety outcomes. We aim to randomise 162 patients over 3 years to achieve 80% power for detecting a 20% difference in the primary outcome at 5% significance.Ethics and disseminationTo date there is no consensus on the optimal protocol for management of subfertile women with adenomyosis. Modified downregulation could improve the clinical pregnancy rate by reducing the endometrial inflammatory reaction and/or myometrial contractility and their impact on uterine receptivity in women with moderate and severe adenomyosis of the uterus undergoing FTET. The MODA trial is designed to offer pragmatic, real-life evaluation of the optimal protocol for downregulation for this population during assisted conception treatments. Our findings will be published in peer-reviewed journals and presented at national and international scientific meetings and congresses. Ethical approval was granted by the NHS Research Ethics Committees (19/LO/1567).Trial registration numberNCT03946722.

Optimization of the process of banana flour preparation

Banana (Musa paradisiaca) is a cheap source of carbohydrates and micronutrients. Converting banana into flour can help to obtain a consistent ingredient for bakery foods while preventing the post-production losses of this bulky and perishable food source. Hence a study has been undertaken to standardize the drying protocol for banana flour preparation. The effects of the different temperature-treatment combination on drying of banana were studied. Experiments were done at 50°C, 60°C and 70°C (without any treatment, with citric acid dipping and with combined citric acid and blanching process) and the variation in the quality of the dehydrated material was studied. The results showed that drying at 70°C followed by 0.5% citric acid treatment for 10 min, in 24h, in non-circulating natural convection air dryer was the optimal protocol concerning better quality parameters like water activity, total soluble solids, ascorbic acid, microbial contamination and the time taken to complete the drying process.