scholarly journals Linkage and Association Mapping of Arabidopsis thaliana Flowering Time in Nature

PLoS Genetics ◽  
2010 ◽  
Vol 6 (5) ◽  
pp. e1000940 ◽  
Author(s):  
Benjamin Brachi ◽  
Nathalie Faure ◽  
Matt Horton ◽  
Emilie Flahauw ◽  
Adeline Vazquez ◽  
...  
Keyword(s):  
Arabidopsis Thaliana ◽  
Association Mapping ◽  
Flowering Time

scholarly journals A novel deletion in FLOWERING LOCUS T modulates flowering time in winter oilseed rape

2021 ◽  
Author(s):  
Paul Vollrath ◽  
Harmeet S. Chawla ◽  
Sarah V. Schiessl ◽  
Iulian Gabur ◽  
HueyTyng Lee ◽  
...  
Keyword(s):  
Association Mapping ◽  
Flowering Time ◽  
Oilseed Rape ◽  
Major Effect ◽  
Structural Variants ◽  
Winter Oilseed Rape ◽  
Flowering Locus T ◽  
Winter Type ◽  
Long Read ◽  
Flowering Locus

Abstract Key message A novel structural variant was discovered in the FLOWERING LOCUS T orthologue BnaFT.A02 by long-read sequencing. Nested association mapping in an elite winter oilseed rape population revealed that this 288 bp deletion associates with early flowering, putatively by modification of binding-sites for important flowering regulation genes. Abstract Perfect timing of flowering is crucial for optimal pollination and high seed yield. Extensive previous studies of flowering behavior in Brassica napus (canola, rapeseed) identified mutations in key flowering regulators which differentiate winter, semi-winter and spring ecotypes. However, because these are generally fixed in locally adapted genotypes, they have only limited relevance for fine adjustment of flowering time in elite cultivar gene pools. In crosses between ecotypes, the ecotype-specific major-effect mutations mask minor-effect loci of interest for breeding. Here, we investigated flowering time in a multiparental mapping population derived from seven elite winter oilseed rape cultivars which are fixed for major-effect mutations separating winter-type rapeseed from other ecotypes. Association mapping revealed eight genomic regions on chromosomes A02, C02 and C03 associating with fine modulation of flowering time. Long-read genomic resequencing of the seven parental lines identified seven structural variants coinciding with candidate genes for flowering time within chromosome regions associated with flowering time. Segregation patterns for these variants in the elite multiparental population and a diversity set of winter types using locus-specific assays revealed significant associations with flowering time for three deletions on chromosome A02. One of these was a previously undescribed 288 bp deletion within the second intron of FLOWERING LOCUS T on chromosome A02, emphasizing the advantage of long-read sequencing for detection of structural variants in this size range. Detailed analysis revealed the impact of this specific deletion on flowering-time modulation under extreme environments and varying day lengths in elite, winter-type oilseed rape.

Design of a Brassica rapa core collection for association mapping studiesThis article is one of a selection of papers from the conference “Exploiting Genome-wide Association in Oilseed Brassicas: a model for genetic improvement of major OECD crops for sustainable farming”.

Genome ◽  
2010 ◽  
Vol 53 (11) ◽  
pp. 884-898 ◽  
Author(s):  
Jianjun Zhao ◽  
Anna Artemyeva ◽  
Dunia Pino Del Carpio ◽  
Ram Kumar Basnet ◽  
Ningwen Zhang ◽  
...  
Keyword(s):  
Association Mapping ◽  
Flowering Time ◽  
Brassica Rapa ◽  
Core Collection ◽  
Field Experiments ◽  
Research Centre ◽  
Candidate Gene Approach ◽  
Core Collections ◽  
Genome Wide ◽  
Using Data

A Brassica rapa collection of 239 accessions, based on two core collections representing different morphotypes from different geographical origins, is presented and its use for association mapping is illustrated for flowering time. We analyzed phenotypic variation of leaf and seed pod traits, plant architecture, and flowering time using data collected from three field experiments and evaluated the genetic diversity with a set of SSR markers. The Wageningen University and Research Centre (WUR) and the Vavilov Research Institute of Plant Industry (VIR) core collections had similar representations of most morphotypes, as illustrated by the phenotypic and genetic variation within these groups. The analysis of population structure revealed five subgroups in the collection, whereas previous studies of the WUR core collection indicated four subgroups; the fifth group identified consisted mainly of oil accessions from the VIR core collection, winter oils from Pakistan, and a number of other types. A very small group of summer oils is described, that is not related to other oil accessions. A candidate gene approach was chosen for association mapping of flowering time with a BrFLC1 biallelic CAPS marker and a BrFLC2 multiallelic SSR marker. The two markers were significantly associated with flowering time, but their effects were confined to certain morphotypes and (or) alleles. Based on these results, we discuss the optimal design for an association mapping population and the need to fix the heterogeneous accessions to facilitate phenotyping and genotyping.

scholarly journals Association mapping reveals gene action and interactions in the determination of flowering time in barley

2008 ◽  
Vol 118 (2) ◽  
pp. 259-273 ◽  
Author(s):  
Silke Stracke ◽  
Grit Haseneyer ◽  
Jean-Baptiste Veyrieras ◽  
Hartwig H. Geiger ◽  
Sascha Sauer ◽  
...  
Keyword(s):  
Association Mapping ◽  
Flowering Time ◽  
Gene Action

The Arabidopsis thaliana adenosine 5’-phosphosulfate reductase 2 (AtAPR2) participates in flowering time and glucose response

2015 ◽  
Vol 58 (2) ◽  
pp. 128-136 ◽  
Author(s):  
Jung-Sung Chung ◽  
Ha-Nul Lee ◽  
Thomas Leustek ◽  
David B. Knaff ◽  
Cheol Soo Kim
Keyword(s):  
Arabidopsis Thaliana ◽  
Flowering Time ◽  
Glucose Response

Alteration in flowering time causes accelerated or decelerated progression through Arabidopsis vegetative phases

2001 ◽  
Vol 79 (6) ◽  
pp. 657-665 ◽  
Author(s):  
Quintin J Steynen ◽  
Dee A Bolokoski ◽  
Elizabeth A Schultz
Keyword(s):  
Arabidopsis Thaliana ◽  
Flowering Time ◽  
Leaf Shape ◽  
Central Mechanism ◽  
Second Phase ◽  
Wild Type ◽  
Low Light ◽  
Terminal Flower ◽  
Vegetative Phases ◽  
Short Days

We have identified three phases within the wild-type Arabidopsis thaliana (L.) Heynh. rosette, based on significant differences in leaf shape, size, vascular pattern, and presence of abaxial trichomes. To test the hypothesis that a single, central mechanism controls the progression through all plant phases and that conditions that alter the time to flowering will also alter the progression through vegetative phases, we analysed the rosette phases under such conditions. In support of our hypothesis, we determined that those conditions (loss of LEAFY activity, short days) that decelerate time to flowering show decelerated progression through the rosette phases, while those conditions (loss of TERMINAL FLOWER, overexpression of LEAFY, low light) that accelerate time to flowering show accelerated progression through the rosette phases. In all conditions except short days, the length of the first phase was unaffected, indicating that this phase is less susceptible to influences of the central mechanism. Progression through the subsequent two rosette phases was accelerated differentially, such that the second phase was affected more strongly than the first. This supports the idea that, in the rosette, as in the inflorescence, the inhibition of phase transition by the central mechanism is gradually decreasing.Key words: phase change, flowering time, Arabidopsis thaliana, LEAFY, TERMINAL FLOWER, heteroblasty.

Carbon nanoparticles influence photomorphogenesis and flowering time in Arabidopsis thaliana

2018 ◽  
Vol 37 (6) ◽  
pp. 901-912 ◽  
Author(s):  
Abhishek Kumar ◽  
Anamika Singh ◽  
Madhusmita Panigrahy ◽  
Pratap Kumar Sahoo ◽  
Kishore C. S. Panigrahi
Keyword(s):  
Arabidopsis Thaliana ◽  
Flowering Time ◽  
Carbon Nanoparticles

scholarly journals BBX32 Interacts with AGL24 Involved in Flowering Time Control in Chinese Cabbage (Brassica rapa L. ssp. pekinensis)

2018 ◽  
Vol 47 (1) ◽  
pp. 34-45
Author(s):  
Guan-Peng MA ◽  
Da-Qin ZHAO ◽  
Tian-Wen WANG ◽  
Lin-Bi ZHOU ◽  
Gui-Lian LI
Keyword(s):  
Arabidopsis Thaliana ◽  
Circadian Clock ◽  
Flowering Time ◽  
Brassica Rapa ◽  
Chinese Cabbage ◽  
Protein Interactions ◽  
Protein Protein Interactions ◽  
Time Control ◽  
Flowering Time Control ◽  
Molecular Regulatory Mechanisms

B-box (BBX) zinc finger proteins play critical roles in both vegetative and reproductive development in plants. Many BBX proteins have been identified in Arabidopsis thaliana as floral transition regulatory factors, such as CO, BBX7 (COL9), BBX19, and BBX32. BBX32 is involved in flowering time control through repression of COL3 in Arabidopsis thaliana, but it is still elusive that whether and how BBX32 directly interacts with flowering signal integrators of AGAMOUS-LIKE 24 (AGL24) and SUPPRESSOR OF OVEREXPRESSION OF CONSTANS 1 (SOC1) in Chinese cabbage (Brassica rapa L. ssp. pekinensis) or other plants. In this study, B-box-32(BBX32), a transcription factor in this family with one B-box motif was cloned from B. rapa, acted as a circadian clock protein, showing expression changes during the circadian period. Additional experiments using GST pull-down and yeast two-hybrid assays indicated that BrBBX32 interacts with BrAGL24 and does not interact with BrSOC1, while BrAGL24 does interact with BrSOC1. To investigate the domains involved in these protein-protein interactions, we tested three regions of BrBBX32. Only the N-terminus interacted with BrAGL24, indicating that the B-box domain may be the key region for protein interaction. Based on these data, we propose that BrBBX32 may act in the circadian clock pathway and relate to the mechanism of flowering time regulation by binding to BrAGL24 through the B-box domain. This study will provide valuable information for unraveling the molecular regulatory mechanisms of BrBBX32 in flowering time of B. rapa.

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