scholarly journals RNA-Seq Reveals Infection-Induced Gene Expression Changes in the Snail Intermediate Host of the Carcinogenic Liver Fluke, Opisthorchis viverrini

2014 ◽  
Vol 8 (3) ◽  
pp. e2765 ◽  
Author(s):  
Sattrachai Prasopdee ◽  
Javier Sotillo ◽  
Smarn Tesana ◽  
Thewarach Laha ◽  
Jutharat Kulsantiwong ◽  
...  
Keyword(s):  
Gene Expression ◽  
Intermediate Host ◽  
Liver Fluke ◽  
Opisthorchis Viverrini ◽  
Rna Seq ◽  
Snail Intermediate Host

Field studies on the transmission of the human liver fluke, Opisthorchis viverrini, in northeast thailand: population changes of the snail intermediate host

1986 ◽  
Vol 16 (5) ◽  
pp. 545-552 ◽  
Author(s):  
Warren Y. Brockelman ◽  
E.Suchart Upatham ◽  
Vithoon Viyanant ◽  
Suraphol Ardsungnoen ◽  
Raat Chantanawat
Keyword(s):  
Intermediate Host ◽  
Human Liver ◽  
Liver Fluke ◽  
Field Studies ◽  
Opisthorchis Viverrini ◽  
Northeast Thailand ◽  
Population Changes ◽  
Snail Intermediate Host

Opisthorchis viverrini: Gene expression profiling of carcinogenic adult liver fluke worms using 5′ SAGE

2008 ◽  
Vol 120 (4) ◽  
pp. 306-313 ◽  
Author(s):  
Nopporn Chutiwitoonchai ◽  
Yan Shen ◽  
Huajun Zheng ◽  
Hui Xiong ◽  
Guoping Zhao ◽  
...  
Keyword(s):  
Gene Expression ◽  
Gene Expression Profiling ◽  
Expression Profiling ◽  
Liver Fluke ◽  
Opisthorchis Viverrini ◽  
Adult Liver

scholarly journals Distribution and habitats of Lymnaea natalensis, snail intermediate host of the liver fluke Fasciola gigantica, in South Africa

2001 ◽  
Vol 20 (2) ◽  
pp. 56-71
Author(s):  
Editorial Office
Keyword(s):  
South Africa ◽  
Intermediate Host ◽  
Geographical Distribution ◽  
Liver Fluke ◽  
Economic Factor ◽  
Freshwater Snail ◽  
Fasciola Gigantica ◽  
Snail Intermediate Host ◽  
Planning And Construction ◽  
Lymnaea Natalensis

This paper focuses on the geographical distribution and the habitats of Lymnaea natalensis, the snail intermediate host of the liver fluke, Fasciola gigantica, as reflected by the collection sites of its 4 552 samples currently on record in the National Freshwater Snail Collection (NFSC) of South Africa. Although this species was represented in a variety of waterbodies, the majority of samples(±70%)came from rivers, brooks and dams and in 70.8% of the cases the water was described as permanent and in 71.8% as slow flowing or standing. The results of life-table studies conducted by various authors indicated that temperature should be a relatively unimportant factor in determining its geographical distribution, but that the availability of permanent water should be decisive for its presence in a given habitat. These results are in agreement with the finding that only 7.5% of the samples of this species in the NFSC were collected in habitats which were described as seasonal. Furthermore, it gives a logical explanation for the sporadic occurrence, or total absence of this species in the more arid regions of South Africa. Water impoundments and irrigation networks contribute to a large extent towards creating perennial habitats which would be suitable for L. natalensis. As intermediate host for one of the liver fluke species which already is an economic factor in South Africa, this certainly is an aspect which ought to be reckoned within the planning and construction of new irrigation schemes.

Species-Specific Primers Designed from RAPD Products forBithynia funiculata,the First Intermediate Host of Liver Fluke,Opisthorchis viverrini, in North Thailand

2013 ◽  
Vol 99 (3) ◽  
pp. 433-437 ◽  
Author(s):  
Jutharat Kulsantiwong ◽  
Sattrachai Prasopdee ◽  
Supawadee Piratae ◽  
Panita Khampoosa ◽  
Apiporn Suwannatrai ◽  
...  
Keyword(s):  
Intermediate Host ◽  
Liver Fluke ◽  
Opisthorchis Viverrini ◽  
Specific Primers ◽  
Species Specific ◽  
North Thailand ◽  
First Intermediate Host

scholarly journals Transcriptomic profiling of three-dimensional cholangiocyte spheroids long term exposed to repetitive Clonorchis sinensis excretory-secretory products

2021 ◽  
Vol 14 (1) ◽  
Author(s):  
Jung-Woong Kim ◽  
Junyeong Yi ◽  
Jinhong Park ◽  
Ji Hoon Jeong ◽  
Jinho Kim ◽  
...  
Keyword(s):  
Gene Expression ◽  
Liver Fluke ◽  
Expression Patterns ◽  
Clonorchis Sinensis ◽  
Three Dimensional ◽  
Global Changes ◽  
Rna Seq ◽  
Extracellular Region ◽  
Secretory Products

Abstract Background Biliary tract infection with the carcinogenic human liver fluke, Clonorchis sinensis, provokes chronic inflammation, epithelial hyperplasia, periductal fibrosis, and even cholangiocarcinoma. Complications are proportional to the intensity and duration of the infection. In addition to mechanical irritation of the biliary epithelia from worms, their excretory-secretory products (ESPs) cause chemical irritation, which leads to inflammation, proliferation, and free radical generation. Methods A three-dimensional in vitro cholangiocyte spheroid culture model was established, followed by ESP treatment. This allowed us to examine the intrinsic pathological mechanisms of clonorchiasis via the imitation of prolonged and repetitive in vivo infection. Results Microarray and RNA-Seq analysis revealed that ESP-treated cholangiocyte H69 spheroids displayed global changes in gene expression compared to untreated spheroids. In ESP-treated H69 spheroids, 185 and 63 probes were found to be significantly upregulated and downregulated, respectively, corresponding to 209 genes (p < 0.01, fold change > 2). RNA-Seq was performed for the validation of the microarray results, and the gene expression patterns in both transcriptome platforms were well matched for 209 significant genes. Gene ontology analysis demonstrated that differentially expressed genes were mainly classified into immune system processes, the extracellular region, and the extracellular matrix. Among the upregulated genes, four genes (XAF1, TRIM22, CXCL10, and BST2) were selected for confirmation using quantitative RT-PCR, resulting in 100% similar expression patterns in microarray and RNA-Seq. Conclusions These findings broaden our understanding of the pathological pathways of liver fluke-associated hepatobiliary disorders and suggest a novel therapeutic strategy for this infectious cancer. Graphic abstract

Morphological and molecular identification of the liver fluke Opisthorchis viverrini in the first intermediate host Bithynia snails and its prevalence in Kampong Cham Province, Cambodia

2017 ◽  
Vol 66 (3) ◽  
pp. 319-323 ◽  
Author(s):  
Dusit Boonmekam ◽  
Suluck Namchote ◽  
Hajime Matsuda ◽  
Masashi Kirinoki ◽  
Kazuko Miyamoto ◽  
...  
Keyword(s):  
Intermediate Host ◽  
Molecular Identification ◽  
Liver Fluke ◽  
Opisthorchis Viverrini ◽  
First Intermediate Host

scholarly journals Verspreiding en habitats van Lymnaea natalensis, tussengasheerslak van die lewerbot Fasciola gigantica, in Suid-Afrika

2001 ◽  
Vol 20 (2) ◽  
pp. 49-53 ◽  
Author(s):  
K. N. De Kock ◽  
C. T. Wolmarans ◽  
H. D. Strauss ◽  
M. Killian
Keyword(s):  
South Africa ◽  
Intermediate Host ◽  
Geographical Distribution ◽  
Liver Fluke ◽  
Economic Factor ◽  
Freshwater Snail ◽  
Fasciola Gigantica ◽  
Snail Intermediate Host ◽  
Planning And Construction ◽  
Lymnaea Natalensis

Hierdie artikel fokus op die geografiese verspreiding en die habitats van Lymnaea natalensis, die slaktussengasheer van die lewerbot, Fasciola gigantica, soos gereflekteer deur die vindplekke van sy 4 552 monsters wat tans in die Nasionale Varswaterslakversameling (NVV) van Suid-Afrika op rekord is. Alhoewel hierdie spesie in’n verskeidenheid van waterliggame aangetref is, was die meerderheid van die monsters (±70%) afkomstig vanuit riviere, spruite en damme en is die water in 70.8% van die gevalle as standhoudend en in 71.8% van die gevalle as stadigvloeiend, of staande beskryf.  Die resultate van lewenstabeleksperimente deur verskeie outeurs het daarop gedui dat temperatuur ’n minder belangrike bepalende faktor in sy geografiese verspreiding blyk te wees, maar dat die beskikbaarheid van standhoudende water deurslaggewend vir sy voorkoms in ’n gegewe habitat mag wees. Hierdie resultate strook met die bevinding dat slegs 7.5% van die monsters van hierdie spesie in die NVV in habitats wat as seisoenaal beskryf is, versamel is. Verder bied dit’n logiese verklaring vir die sporadiese voorkoms, of totale afwesigheid van hierdie spesie in die droër streke van Suid-Afrika.  Opgaardamme en besproeiingsnetwerke dra grootliks by tot die skepping van permanente habitats wat geskik sou wees vir L. natalensis.  As tussengasheer vir een van die lewerbotspesies wat reeds ’n ekonomiese faktor in Suid-Afrika is, is dit ’n aspek waarmee beslis rekening gehou behoort te word in die beplanning en konstruksie van nuwe besproeiingsprojekte.AbstractDistribution and habitats of Lymnaea natalensis, snail intermediate host of the liver fluke Fasciola gigantica, in South Africa This paper focuses on the geographical distribution and the habitats of Lymnaea natalensis, the snail intermediate host of the liver fluke, Fasciola gigantica, as reflected by the collection sites of its 4 552 samples currently on record in the National Freshwater Snail Collection (NFSC) of South Africa.  Although this species was represented in a variety of waterbodies, the majority of samples (±70%) came from rivers, brooks and dams and in 70.8% of the cases the water was described as permanent and in 71.8% as slow flowing or standing.  The results of life-table studies conducted by various authors indicated that temperature should be a relatively unimportant factor in determining its geographical distribution, but that the availability of permanent water should be decisive for its presence in a given habitat.  These results are in agreement with the finding that only 7.5% of the samples of this species in the NFSC were collected in habitats which were described as seasonal.  Furthermore, it gives a logical explanation for the sporadic occurrence, or total absence of this species in the more arid regions of South Africa. Water impoundments and irrigation networks contribute to a large extent towards creating perennial habitats which would be suitable for L. natalensis.  As intermediate host for one of the liver fluke species which already is an economic factor in South Africa, this certainly is an aspect which ought to be reckoned with in the planning and construction  of new irrigation schemes.

Effects of Bayluscide on Bithynia siamensis goniomphalos, the first intermediate host of the human liver fluke, Opisthorchis viverrini, in laboratory and field trials

2012 ◽  
Vol 61 (1) ◽  
pp. 52-55 ◽  
Author(s):  
S. Tesana ◽  
P. Thapsripair ◽  
C. Thammasiri ◽  
S. Prasopdee ◽  
A. Suwannatrai ◽  
...  
Keyword(s):  
Intermediate Host ◽  
Human Liver ◽  
Liver Fluke ◽  
Field Trials ◽  
Opisthorchis Viverrini ◽  
First Intermediate Host ◽  
Bithynia Siamensis Goniomphalos
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