Acanthoparyphium shinanense n. sp. (Digenea: Echinostomatidae) from Experimental Chicks Infected with Metacercariae Encysted in Brackish Water Clams in the Republic of Korea

Acanthoparyphium shinanense n. sp. (Digenea: Echinostomatidae) is described from chicks experimentally infected with the metacercariae encysted in 2 brackish water clam species, Ruditapes philippinarum and Coecella chinensis, in the Republic of Korea. The metacercariae were round to oval, armed with 23 collar spines, and 0.216 (0.203-0.226) mm in diameter. From 5 chicks experimentally infected each with 200 metacercariae, 34 juvenile (5-day-old worms) and 104 adult flukes (7-day-old worms) were harvested from their small intestines, with the average worm recovery rate of 13.8%. The adult flukes were 3.18 (2.89-3.55) mm long and 0.68 (0.61-0.85) mm wide, with an elongated, posteriorly tapering body, and a prominent head collar armed with 23 collar spines arranged in a single uninterrupted row. The posterior testis of A. shinanense was longitudinally elongated, which is similar to Acanthoparyphium spinulosum Johnston, 1917 but unique from the other closely related species, including Acanthoparyphium tyosenense Yamaguti, 1939, Acanthoparyphium kurogamo Yamaguti, 1939, and Acanthoparyphium marilae Yamaguti, 1934. The eggs of A. shinanense were larger than those of A. spinulosum, and the anterior extent of 2 lateral groups of vitellaria was slightly more limited in A. shinanense than in A. spinulosum. Molecular analysis of nuclear and mitochondrial genes revealed low homology with A. spinulosum from USA (96.1% in 5.8S rRNA) and Ukraine (97.9% in 28S rRNA), Acanthoparyphium n. sp. from USA (98.0% in 28S rRNA), and Acanthoparyphium sp. from Australia, Kuwait, and New Zealand. Biological characteristics, including its first intermediate host and natural definitive hosts, as well as its zoonotic capability, should be elucidated.

THE PROBLEM OF BERDSKY BAY OF THE NOVOSIBIRSK RESERVOIR IN RELATION TO OPISTHORCHIDOSES

We studied the epidemic situation of opisthorchiasis in the population of Berdsk, and determined the level of infection of fish of the family Cyprinidae by larvae of opisthorchides in Berdsky Bay, as well as the abundance of the first intermediate host of the parasite, the mollusks of the family Bithyniidae in the water body. It is found that Berdsky Bay is generally unfavorable for opisthorchiasis. The incidence of opisthorchiasis in people in Berdsk was recorded annually; the average long-term incidence rate was 89.85 per 100 thousand of population with a minimum of 21.4 in 1991 and a maximum of 188.5 in 2002. The level of infection of cyprinid fish with metacercarias of opisthorchides is quite high (33.3%).The epidemic danger is posed by fish infected with larvae of Opisthorchis felineus (the prevalence 31.7%) and Metorchis bilis (the prevalence 3.3%). The ide, dace and verkhovka represent a high potential danger in infecting the population with opisthorchiasis and are indicators of the adverse situation with the reservoir as regards the invasion. In the water area of Berdsky Bay, we identified new actively functioning foci of opisthorchis invasion located in the Lebedevskaya and Elbanskaya streams, as well as in the mouth of the Sukhaya River where not only infected fish were recorded, but also the number of the first intermediate host of opisthorchides, the mollusks of the family Bithyniidae (Bithynia troscheli and B. tentaculata), is quite high.

A new schistosome (Digenea: Schistosomatidae) from the nasal tissue of South America black-necked swans, Cygnus melancoryphus (Anatidae) and the endemic pulmonate snail Chilina gibbosa

To date, 9 species of Schistosomatidae have been found parasitizing the nasal tissues of mammal and bird hosts in the Eastern Hemisphere, 5 species in Rwanda (Africa), 2 in Australia (Oceania) and 2 in Eurasia. During a parasitological survey of black necked swans, Cygnus melancoryphus, an anatid endemic to South America, schistosome worms in the nasal tissue were found; the first in the Americas. Morphological results based on male worms and in isolated eggs. The worms have a spiny tegument, filiform body with rounded posterior end, two muscular suckers, a robust gynaecophoric channel with thickened cross bands, and around 130 testes. The eggs are elongate with an asymmetrical bulge, with a slender process at one end and a longer curved process at the other. Diagnostic morphological characteristics do not match with any schistosome genus. Part of the mitochondrial cox1 and nuclear DNA 28S partial genes were sequenced and compared to Schistosomatidae in GenBank. The genetic results confirm the distinctiveness of the specimens since they do not group with any described genus or undescribed lineage other than cercariae of “Chilina lineage 1” that emerge from the Patagonian Chilina gibbosa, a freshwater snail endemic to South America. Based on morphological and genetic characterization of these schistosomes, these specimens represent a new genus and species that parasitizes black necked swans as adults in the nasal tissue, and C. gibbosa is the first intermediate host, both hosts being endemic to South America.

A month for the mission: using a sentinel approach to determine the transmission window of digenean cercariae in the subarctic White Sea

In the digenean life cycle the cercaria ensures an important transmission stage, from the first intermediate host to the second or the definitive host. In regions with pronounced seasonality, this process occurs within a certain interval, the transmission window. In high latitudes, the size of transmission window has previously been determined only by comparing data on seasonal dynamics of infection level in various categories of hosts or extrapolating the results of laboratory experiments on cercarial biology to natural conditions. In this study, we evaluated the dynamics of infection of the second intermediate hosts (mussels Mytilus edulis) with cercariae of two digenean species, Himasthla elongata (Himasthlidae) and Cercaria parvicaudata (Renicolidae), at a littoral site at the White Sea by exposing cages with uninfected mussels during the warm season. This is the first such study in a subarctic sea. Mussel infection was observed from May to mid-September, but its intensity was the greatest only for approximately a month, from some moment after 10 July to mid-August, when water temperature was within the optimal range for cercarial emergence in both studied species (15‒20°C). During this time, the mussels accumulated 66.3 ± 6.2% metacercariae of H. elongata and 79.7 ± 5.3% metacercariae of C. parvicaudata out of the total number accumulated during the experimental period. We suggest that climate warming at high latitudes may prolong the period when the water temperatures are optimal for cercariae emergence, thereby intensifying digenean transmission in coastal ecosystems.

Effects of first intermediate host density, host size and salinity on trematode infections in mussels of the south-western Baltic Sea

Trematode prevalence and abundance in hosts are known to be affected by biotic drivers as well as by abiotic drivers. In this study, we used the unique salinity gradient found in the south-western Baltic Sea to: (i) investigate patterns of trematode infections in the first intermediate host, the periwinkle Littorina littorea and in the downstream host, the mussel Mytilus edulis, along a regional salinity gradient (from 13 to 22) and (ii) evaluate the effects of first intermediate host (periwinkle) density, host size and salinity on trematode infections in mussels. Two species dominated the trematode community, Renicola roscovita and Himasthla elongata. Salinity, mussel size and density of infected periwinkles were significantly correlated with R. roscovita, and salinity and density correlated with H. elongata abundance. These results suggest that salinity, first intermediate host density and host size play an important role in determining infection levels in mussels, with salinity being the main major driver. Under expected global change scenarios, the predicted freshening of the Baltic Sea might lead to reduced trematode transmission, which may be further enhanced by a potential decrease in periwinkle density and mussel size.

FIRST INTERMEDIATE HOST ALARIA ALATA (TREMATODA, STRIGEIDIDA) IN THE NATURAL CONDITIONS OF THE CENTRAL BLACK EARTH REGION

Currently, an increase has been noted in the number of cases of dogs infected with Alaria alata (Trematoda, Srigeidida) in the Central Black Earth Region (Voronezh and Lipetsk Regions). However, individual links are not known, primarily, the first intermediate host involved in the life cycle and circulation of A. alata in natural conditions. New data were obtained on the implementation of the life cycle of A. alata on the study territory. The mollusk Planorbis planorbis was registered as the first intermediate host 1.5% (8 specimens) were identified as infected with parthenita A. alata among the studied mollusk Planorbis (527 specimens). Cercariae were investigated by micromorphological methods, and the comparative analysis allows them to be classified as A. alata. The cercariae belong to the group of furcocercous cercaria Furcocercaria, the tail stem at the distal end forms a furca. The tail stem has 14 pairs of short hairs. The tail rami have small spinelets and carry three pairs of short hairs. When the excretory system was studied, a transverse commissure was not detected. Unpigmented eyes are located in the front part of the body at the edge. Alariosis circulates as a natural focal parasitosis in the Central Black Earth Region.

Cost of interspecific competition between trematode colonies

In a range of trematode species, specific members of the parthenitae colony infecting the molluscan first intermediate host appear specialized for defence against co-infecting species. The evolution of such division of labour requires that co-infection entails fitness costs. Yet, this premise has very rarely been tested in species showing division of labour. Using Himasthla elongata (Himasthlidae) and Renicola roscovita (Renicolidae) infecting periwinkles Littorina littorea as study system, we show that the size of emerged cercariae is markedly reduced in both parasite species when competing over host resources. Cercarial longevity, on the other hand, is negatively influenced by competition only in R. roscovita. Season, which may impact the nutritional state of the host, also affects cercarial size, but only in H. elongata. Hence, our study underlines that cercarial quality is, indeed, compromised by competition, not only in the inferior R. roscovita (no division of labour) but also in the competitively superior H. elongata (division of labour).