scholarly journals Campylobacter jejuni Colonization in Wild Birds: Results from an Infection Experiment

PLoS ONE ◽  
2010 ◽  
Vol 5 (2) ◽  
pp. e9082 ◽  
Author(s):  
Jonas Waldenström ◽  
Diana Axelsson-Olsson ◽  
Björn Olsen ◽  
Dennis Hasselquist ◽  
Petra Griekspoor ◽  
...  
Keyword(s):  
Campylobacter Jejuni ◽  
Wild Birds ◽  
Infection Experiment

scholarly journals Population Genetics and Characterization ofCampylobacter jejuniIsolates from Western Jackdaws and Game Birds in Finland

2018 ◽  
Vol 85 (4) ◽  
Author(s):  
Sara Kovanen ◽  
Mirko Rossi ◽  
Mari Pohja-Mykrä ◽  
Timo Nieminen ◽  
Mirja Raunio-Saarnisto ◽  
...  
Keyword(s):  
Host Specificity ◽  
Campylobacter Jejuni ◽  
Multilocus Sequence Typing ◽  
Wild Birds ◽  
Whole Genome ◽  
Mallard Duck ◽  
Content Type ◽  
Game Birds ◽  
Hygienic Measures ◽  
Sequence Types

ABSTRACTPoultry are considered a major reservoir and source of human campylobacteriosis, but the roles of environmental reservoirs, including wild birds, have not been assessed in depth. In this study, we isolated and characterizedCampylobacter jejunifrom western jackdaws (n= 91, 43%), mallard ducks (n= 82, 76%), and pheasants (n= 9, 9%). Most of the western jackdaw and mallard duckC. jejuniisolates represented multilocus sequence typing (MLST) sequence types (STs) that diverged from those previously isolated from human patients and various animal species, whereas all pheasant isolates represented ST-19, a common ST among human patients and other hosts worldwide. Whole-genome MLST revealed that mallard duck ST-2314 and pheasant ST-19 isolates represented bacterial clones that were genetically highly similar to human isolates detected previously. Further analyses revealed that in addition to a divergent ClonalFrame genealogy, certain genomic characteristics of the western jackdawC. jejuniisolates, e.g., a novelcdtABCgene cluster and the type VI secretion system (T6SS), may affect their host specificity and virulence. Game birds may thus pose a risk for acquiring campylobacteriosis; therefore, hygienic measures during slaughter and meat handling warrant special attention.IMPORTANCEThe roles of environmental reservoirs, including wild birds, in the molecular epidemiology ofCampylobacter jejunihave not been assessed in depth. Our results showed that game birds may pose a risk for acquiring campylobacteriosis, because they hadC. jejunigenomotypes highly similar to human isolates detected previously. Therefore, hygienic measures during slaughter and meat handling warrant special attention. On the contrary, a unique phylogeny was revealed for the western jackdaw isolates, and certain genomic characteristics identified among these isolates are hypothesized to affect their host specificity and virulence. Comparative genomics within sequence types (STs), using whole-genome multilocus sequence typing (wgMLST), and phylogenomics are efficient methods to analyze the genomic relationships ofC. jejuniisolates.

scholarly journals Emergence of Genetic Diversity and Multi-Drug Resistant Campylobacter jejuni From Wild Birds in Beijing, China

2019 ◽  
Vol 10 ◽  
Author(s):  
Juan Du ◽  
Jing Luo ◽  
Jingjing Huang ◽  
Chengmin Wang ◽  
Meng Li ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Campylobacter Jejuni ◽  
Wild Birds ◽  
Drug Resistant ◽  
Beijing China

scholarly journals Molecular Epidemiology of Campylobacter jejuni Isolates from Wild-Bird Fecal Material in Children's Playgrounds

2008 ◽  
Vol 75 (3) ◽  
pp. 779-783 ◽  
Author(s):  
Nigel P. French ◽  
Anne Midwinter ◽  
Barbara Holland ◽  
Julie Collins-Emerson ◽  
Rebecca Pattison ◽  
...  
Keyword(s):  
New Zealand ◽  
Campylobacter Jejuni ◽  
Wild Bird ◽  
Wild Birds ◽  
Fecal Material ◽  
Bird Populations ◽  
The United Kingdom ◽  
Garden Birds ◽  
The 19Th Century ◽  
Children Under 5

ABSTRACT In many countries relatively high notification rates of campylobacteriosis are observed in children under 5 years of age. Few studies have considered the role that environmental exposure plays in the epidemiology of these cases. Wild birds inhabit parks and playgrounds and are recognized carriers of Campylobacter, and young children are at greater risk of ingesting infective material due to their frequent hand-mouth contact. We investigated wild-bird fecal contamination in playgrounds in parks in a New Zealand city. A total of 192 samples of fresh and dried fecal material were cultured to determine the presence of Campylobacter spp. Campylobacter jejuni isolates were also characterized by pulsed-field gel electrophoresis (PFGE) and multilocus sequence typing (MLST), and the profiles obtained were compared with those of human isolates. C. jejuni was isolated from 12.5% of the samples. MLST identified members of clonal complexes ST-45, ST-682, and ST-177; all of these complexes have been recovered from wild birds in Europe. PFGE of ST-45 isolates resulted in profiles indistinguishable from those of isolated obtained from human cases in New Zealand. Members of the ST-177 and ST-682 complexes have been found in starlings (Sturnus vulgaris) in the United Kingdom, and these birds were common in playgrounds investigated in New Zealand in this study. We suggest that feces from wild birds in playgrounds could contribute to the occurrence of campylobacteriosis in preschool children. Further, the C. jejuni isolates obtained in this study belonged to clonal complexes associated with wild-bird populations in the northern hemisphere and could have been introduced into New Zealand in imported wild garden birds in the 19th century.

scholarly journals Prevalence and Genetic Diversity of C. Jejuni Isolated from Broilers and their Environment Using flaA-RFLP Typing and MLST Analysis

2020 ◽  
Vol 20 (2) ◽  
pp. 485-501
Author(s):  
Viktorija Lėgaudaitė Lydekaitienė ◽  
E. Kudirkienė
Keyword(s):  
Genetic Diversity ◽  
Restriction Fragment Length Polymorphism ◽  
Campylobacter Jejuni ◽  
Length Polymorphism ◽  
Fragment Length Polymorphism ◽  
Wild Birds ◽  
Mlst Analysis ◽  
Sequence Types ◽  
Restriction Fragment Length ◽  
Rflp Typing

AbstractCampylobacter is highly diverse genetically and also undergoes frequent intraspecific recombination. A major source of campylobacteriosis, which is transmitted to humans is found in poultry. The assessment of the genetic diversity among Campylobacter population is critical to our understanding of the epidemiology. The genetic diversity of Campylobacter jejuni isolates in broilers and their environment were investigated by flaA-restriction fragment length polymorphism (RFLP) and multilocus sequence typing (MLST). The study revealed that 92.3% of the examined broiler flocks were contaminated with Campylobacter spp. A total number of 35 different flaA types defined by flaA-RFLP were found in 448 C. jejuni isolates originated from broilers, litter, puddles, zones, anteroom and wild birds. The most dominant flaA type was XXV. MLST defined 20 sequence types (STs) belonging to 10 clonal complexes (CCs). Among all the STs 9 isolates (15%) were consigned to 2 different STs (ST-7413 and ST-4800), which could not be assigned. The most common CCs were ST-21 and ST-179. The ST-21 CC was common in broilers and environment (puddle water and concentric zones) and the ST-179 CC was specific to wild birds, but also was found in puddle water and concentric zones.

Campylobacter jejuni in a Washington State Shellfish Growing Bed Associated With Illness

1993 ◽  
Vol 56 (4) ◽  
pp. 323-325 ◽  
Author(s):  
CARLOS ABEYTA ◽  
FRANK G. DEETER ◽  
CHARLES A. KAYSNER ◽  
ROBERT F. STOTT ◽  
MARLEEN M. WEKELL
Keyword(s):  
Campylobacter Jejuni ◽  
Marine Environment ◽  
Puget Sound ◽  
Washington State ◽  
The State ◽  
Wild Birds ◽  
Pacific Oysters ◽  
Duration Of Illness ◽  
Campylobacter Species

Consumption of raw Pacific oysters (Crassotea gigas) harvested from a Washington State recreational shellfish bed were associated with illness. Illness occurred within 2 d of ingestion of a half-dozen shellstock oysters. Each oyster consist of approximately 20 g of meat. The duration of illness lasted 2 d. Routinely, Campylobacter species have been found in several shellfish beds in the Puget Sound Bay. Its presence in the marine environment appears to be incidental and primarily, comes from wild birds, farm runoff, and sewage bypasses. This paper describes the first reported case of Campylobacter gastroenteritis associated with raw oyster consumption in the State of Washington.

Prevalence of Campylobacter jejuni in Selected Domestic and Wild Birds in Louisiana

1989 ◽  
Vol 33 (4) ◽  
pp. 664 ◽  
Author(s):  
K. Yogasundram ◽  
S. M. Shane ◽  
K. S. Harrington
Keyword(s):  
Campylobacter Jejuni ◽  
Wild Birds

scholarly journals Overlap of Antibiotic Resistant Campylobacter jejuni MLST Genotypes Isolated From Humans, Broiler Products, Dairy Cattle and Wild Birds in Lithuania

2019 ◽  
Vol 10 ◽  
Author(s):  
Jurgita Aksomaitiene ◽  
Sigita Ramonaite ◽  
Egle Tamuleviciene ◽  
Aleksandr Novoslavskij ◽  
Thomas Alter ◽  
...  
Keyword(s):  
Dairy Cattle ◽  
Campylobacter Jejuni ◽  
Wild Birds ◽  
Antibiotic Resistant

scholarly journals Campylobacter jejuni Strains Associated with Wild Birds and Those Causing Human Disease in Six High-Use Recreational Waterways in New Zealand

2019 ◽  
Vol 85 (24) ◽  
Author(s):  
Rima D. Shrestha ◽  
Anne C. Midwinter ◽  
Jonathan C. Marshall ◽  
Julie M. Collins-Emerson ◽  
Eve J. Pleydell ◽  
...  
Keyword(s):  
Public Health ◽  
New Zealand ◽  
Campylobacter Jejuni ◽  
Human Disease ◽  
River Flow ◽  
Human Infection ◽  
Wild Birds ◽  
Recreational Waters ◽  
Content Type ◽  
Sequence Types

ABSTRACT Campylobacter jejuni, a leading cause of gastroenteritis worldwide, has been frequently isolated from recreational rivers and streams in New Zealand, yet the public health significance of this is unknown. This study uses molecular tools to improve our understanding of the epidemiology and sources of Campylobacter in recreational waterways, with a view to preventing human infection. Epidemiological and microbiological data were collected between 2005 and 2009 from six high-use recreational waterways in the Manawatu-Wanganui region of the North Island. Campylobacter spp. and C. jejuni were isolated from 33.2% and 20.4% of 509 samples, respectively. Isolation of Campylobacter was observed in both low and high river flows. After adjusting for the confounding effects of river flow, there was a significantly higher likelihood of isolating Campylobacter in the winter month of June compared to January. A high diversity of C. jejuni multilocus sequence types was seen, with the most commonly isolated being the water rail-associated ST-2381 (19/91 isolates [20.9%]), ST-1225 (8/91 isolates [8.8%]), and ST-45 (6/91 isolates [6.6%]). The ST-2381 was found in all rivers, while the most commonly isolated ST from human cases in New Zealand, the poultry-associated strain ST-474, was isolated only in one river. Although the majority of Campylobacter sequence types identified in river water were strains associated with wild birds that are rarely associated with human disease, poultry and ruminant-associated Campylobacter strains that are found in human infection were also identified and could present a public health risk. IMPORTANCE In 2016, there was a large-scale waterborne outbreak of campylobacteriosis in New Zealand, which was estimated to have affected over 5,000 people. This highlighted the need for a greater understanding of the sources of contamination of both surface and groundwater and risks associated with exposure to both drinking and recreational water. This study reports the prevalence and population structure of Campylobacter jejuni in six recreational waters of the Manawatu-Wanganui region of New Zealand and models the relationship between Campylobacter spp. and ruminant-associated Campylobacter and the parameters “sites,” “months,” and “river flow.” Here, we demonstrate that both low and high river flows, month of the year, and recreational sites could influence the Campylobacter isolation from recreational waters. The presence of genotypes associated with human infection allowed us to describe potential risks associated with recreational waters.

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