Differential Proteomic Analysis of Arabidopsis thaliana Genotypes Exhibiting Resistance or Susceptibility to the Insect Herbivore, Plutella xylostella

Chloroplast biogenesis involves the coordinated expression of the plastid and nuclear genomes, requiring information to be sent from the nucleus to the developing chloroplasts and vice versa. Although it is well known how the nucleus controls chloroplast development, it is still poorly understood how the plastid communicates with the nucleus. Currently, haem is proposed as a plastid-to-nucleus (retrograde) signal that is involved in various physiological regulations, such as photosynthesis-associated nuclear genes expression and cell cycle in plants and algae. However, components that transduce haem-dependent signalling are still unidentified. In this study, by using haem-immobilized high-performance affinity beads, we performed proteomic analysis of haem-binding proteins from Arabidopsis thaliana and Cyanidioschyzon merolae . Most of the identified proteins were non-canonical haemoproteins localized in various organelles. Interestingly, half of the identified proteins were nucleus proteins, some of them have a similar function or localization in either or both organisms. Following biochemical analysis of selective proteins demonstrated haem binding. This study firstly demonstrates that nucleus proteins in plant and algae show haem-binding properties. This article is part of the theme issue ‘Retrograde signalling from endosymbiotic organelles’.

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Protein Preparation for Proteomic Analysis of the Unfolded Protein Response in Arabidopsis thaliana

Methods in Molecular Biology - The Unfolded Protein Response ◽

10.1007/978-1-0716-1732-8_18 ◽

2022 ◽

pp. 279-289

Author(s):

Yunting Pu ◽

Federica Brandizzi

Keyword(s):

Arabidopsis Thaliana ◽

Unfolded Protein Response ◽

Proteomic Analysis ◽

Protein Preparation ◽

Unfolded Protein ◽

The Unfolded Protein Response ◽

Protein Response

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Proteomic Analysis of Glutathione S-Transferases of Arabidopsis thaliana Reveals Differential Salicylic Acid-Induced Expression of the Plant-Specific Phi and Tau Classes

Plant Molecular Biology ◽

10.1023/b:plan.0000028786.57439.b3 ◽

2004 ◽

Vol 54 (2) ◽

pp. 205-219 ◽

Cited By ~ 74

Author(s):

Pia G. Sappl ◽

Luis Oñate-Sánchez ◽

Karam B. Singh ◽

A. Harvey Millar

Keyword(s):

Arabidopsis Thaliana ◽

Salicylic Acid ◽

Proteomic Analysis ◽

Induced Expression ◽

Glutathione S Transferases

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Proteomic Analysis of Interactions Between the Generalist Herbivore Spodoptera exigua (Lepidoptera: Noctuidae) and Arabidopsis thaliana

Plant Molecular Biology Reporter ◽

10.1007/s11105-009-0156-6 ◽

2009 ◽

Vol 28 (2) ◽

pp. 324-333 ◽

Cited By ~ 16

Author(s):

Ju-Hong Zhang ◽

Li-Wen Sun ◽

Lin-Lin Liu ◽

Jie Lian ◽

Shao-Li An ◽

...

Keyword(s):

Arabidopsis Thaliana ◽

Proteomic Analysis ◽

Spodoptera Exigua ◽

Generalist Herbivore ◽

Lepidoptera Noctuidae

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Proteomic analysis of Arabidopsis thaliana leaves infested by tobacco whitefly Bemisia tabaci (Gennadius) B biotype

Plant Molecular Biology Reporter ◽

10.1007/s11105-011-0351-0 ◽

2011 ◽

Vol 30 (2) ◽

pp. 379-390 ◽

Cited By ~ 7

Author(s):

Haidi Yin ◽

Fengming Yan ◽

Jianguo Ji ◽

Yinxin Li ◽

Rongjiang Wang ◽

...

Keyword(s):

Arabidopsis Thaliana ◽

Bemisia Tabaci ◽

Proteomic Analysis ◽

Tobacco Whitefly ◽

B Biotype

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Proteomic analysis of differentially expressed proteins in the roots of Columbia-0 and Landsberg erecta ecotypes of Arabidopsis thaliana in response to aluminum toxicity

Canadian Journal of Plant Science ◽

10.4141/cjps2012-098 ◽

2012 ◽

Vol 92 (7) ◽

pp. 1267-1282 ◽

Cited By ~ 13

Author(s):

T. Karuppanapandian ◽

S-J. Rhee ◽

E-J. Kim ◽

B. K. Han ◽

O. A. Hoekenga ◽

...

Keyword(s):

Arabidopsis Thaliana ◽

Stress Responses ◽

Proteomic Analysis ◽

Crop Production ◽

Aluminum Toxicity ◽

Expression Patterns ◽

Differentially Expressed ◽

Monodehydroascorbate Reductase ◽

Differentially Expressed Proteins ◽

Al Stress

Karuppanapandian, T., Rhee, S.-J., Kim, E.-J., Han, B. K., Hoekenga, O. A. and Lee, G. P. 2012. Proteomic analysis of differentially expressed proteins in the roots of Columbia-0 and Landsberg erecta ecotypes of Arabidopsis thaliana in response to aluminum-toxicity. Can. J. Plant Sci. 92: 1267–1282. Aluminum (Al) is phytotoxic when solubilized into Al3+ in acidic soils and represents a major constraint for crop production. The present study describes Al-stress responses in roots of Al-tolerant and Al-sensitive Arabidopsis ecotypes, Columbia-0 (Col-0) and Landsberg erecta (Ler), respectively. Comparative proteomic analysis was applied to plants grown in hydroponic solution culture under acidic pH (4.2) conditions. To investigate time-dependent responses, 6-d-old seedlings were treated with 30 µM AlCl3 for 24, 48, or 72 h; total proteins were prepared from roots and separated by two-dimensional gel electrophoresis (2-DE). From 2-DE analysis, were 600 proteins were inspected, 29 proteins were differentially responsive to Al-treatment. The 2-DE patterns were compared and differentially expressed proteins identified by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF-MS). Analysis of protein expression patterns revealed that a set of proteins is functionally associated with tricarboxylic acid (TCA) cycle and glycolysis, reactive oxygen quenching and detoxification mechanism, and signal transduction pathways, etc., could play important roles in mediating plant response to Al in Arabidopsis ecotypes. Comparison of the changes in the protein profiles revealed that Al-stress increased Al-tolerance related proteins in Al-tolerant Col-0, but only generic stress responses occurred in Al-sensitive Ler. Specifically, Al up-regulated proteins such as alcohol dehydrogenase, monodehydroascorbate reductase, GTP-binding nuclear protein Ran-2, and leucine aminopeptidase in Col-0 but not in Ler.

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AtRsgA from Arabidopsis thaliana controls maturation of the small subunit of the chloroplast ribosome

10.1101/225052 ◽

2017 ◽

Cited By ~ 1

Author(s):

Marcin Janowski ◽

Reimo Zoschke ◽

Lars Scharff ◽

Silvia Martinez Jaime ◽

Camilla Ferrari ◽

...

Keyword(s):

Arabidopsis Thaliana ◽

Proteomic Analysis ◽

Ribosome Biogenesis ◽

Functional Characterization ◽

Small Subunit ◽

Signalling Pathways ◽

Compensatory Mechanism ◽

Transcriptional Responses ◽

Retrograde Signalling ◽

Assembly Factor

SummaryPlastid ribosomes are very similar in structure and function to ribosomes of their bacterial ancestors. Since ribosome biogenesis is not thermodynamically favourable at biological conditions, it requires activity of many assembly factors. Here, we have characterized a homolog of bacterial rsgA in Arabidopsis thaliana and show that it can complement the bacterial homolog. Functional characterization of a strong mutant in Arabidopsis revealed that the protein is essential for plant viability, while a weak mutant produced dwarf, chlorotic plants that incorporated immature pre-16S ribosomal RNA into translating ribosomes. Physiological analysis of the mutant plants revealed smaller, but more numerous chloroplasts in the mesophyll cells, reduction of chlorophyll a and b, depletion of proplastids from the rib meristem and decreased photosynthetic electron transport rate and efficiency. Comparative RNA-sequencing and proteomic analysis of the weak mutant and wild-type plants revealed that various biotic stress-related, transcriptional regulation and post-transcriptional modification pathways were repressed in the mutant. Intriguingly, while nuclear- and chloroplast-encoded photosynthesis-related proteins were less abundant in the mutant, the corresponding transcripts were upregulated, suggesting an elaborate compensatory mechanism, potentially via differentially active retrograde signalling pathways. To conclude, this study reveals a new chloroplast ribosome assembly factor and outlines the transcriptomic and proteomic responses of the compensatory mechanism activated during decreased chloroplast function.Significance statementAtRsgA is an assembly factor necessary for maturation of the small subunit of the chloroplast ribosome. Depletion of AtRsgA leads to dwarfed, chlorotic plants and smaller, but more numerous chloroplasts. Large-scale transcriptomic and proteomic analysis revealed that chloroplast-encoded and - targeted proteins were less abundant, while the corresponding transcripts were upregulated in the mutant. We analyse the transcriptional responses of several retrograde signalling pathways to suggest a mechanism underlying this compensatory response.

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