MLVA Genotyping of Brucella melitensis and Brucella abortus Isolates from Different Animal Species and Humans and Identification of Brucella suis Vaccine Strain S2 from Cattle in China

Brucella-phages-adsorption test on killed (70°) cells of all types of Brucella in the pure “S” phase has orientating value for determination of the Genus: “Brucella”.Brucella abortus “S” strains are in 100% positive in this test. Brucella melitensis “S” strains are in 83% positive and in 17% weakly positive.Brucella suis “S” strains are in 100% positive.Brucella atypica “S” strains are mostly positive. All types of Brucella in “R” phase are negative in this test. This experiment is important from point of view of general systematics of bacteria and for the explanation of phage-specific receptors in the “S” and “R” phase.

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ATP-Binding Cassette Systems ofBrucella

Comparative and Functional Genomics ◽

10.1155/2009/354649 ◽

2009 ◽

Vol 2009 ◽

pp. 1-16 ◽

Cited By ~ 11

Author(s):

Dominic C. Jenner ◽

Elie Dassa ◽

Adrian M. Whatmore ◽

Helen S. Atkins

Keyword(s):

Middle East ◽

South America ◽

Virulence Factors ◽

Brucella Abortus ◽

Brucella Melitensis ◽

Human Brucellosis ◽

Brucella Ovis ◽

Brucella Canis ◽

The World ◽

Brucella Suis

Brucellosis is a prevalent zoonotic disease and is endemic in the Middle East, South America, and other areas of the world. In this study, complete inventories of putative functional ABC systems of fiveBrucellaspecies have been compiled and compared. ABC systems ofBrucella melitensis16M,Brucella abortus9-941,Brucella canisRM6/66,Brucella suis1330, andBrucella ovis63/290 were identified and aligned. High numbers of ABC systems, particularly nutrient importers, were found in allBrucellaspecies. However, differences in the total numbers of ABC systems were identified (B. melitensis, 79;B. suis, 72;B. abortus64;B. canis, 74;B. ovis, 59) as well as specific differences in the functional ABC systems of theBrucellaspecies. SinceB. ovisis not known to cause human brucellosis, functional ABC systems absent in theB. ovisgenome may represent virulence factors in human brucellosis.

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Real-time detection of Brucella abortus, Brucella melitensis and Brucella suis

Molecular and Cellular Probes ◽

10.1006/mcpr.2000.0338 ◽

2001 ◽

Vol 15 (1) ◽

pp. 43-52 ◽

Cited By ~ 67

Author(s):

R. Redkar ◽

S. Rose ◽

B. Bricker ◽

V. DelVecchio

Keyword(s):

Real Time ◽

Brucella Abortus ◽

Brucella Melitensis ◽

Brucella Suis ◽

Real Time Detection

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OBSERVATIONS ON THE INFECTION OF CHICK EMBRYOS WITH BACTERIUM TULARENSE, BRUCELLA, AND PASTEURELLA PESTIS

Journal of Experimental Medicine ◽

10.1084/jem.74.3.213 ◽

1941 ◽

Vol 74 (3) ◽

pp. 213-222 ◽

Cited By ~ 28

Author(s):

G. John Buddingh ◽

Frank C. Womack

Keyword(s):

Epithelial Cells ◽

Chick Embryo ◽

Vascular Endothelium ◽

Brucella Abortus ◽

Brucella Melitensis ◽

Chick Embryos ◽

Intracellular Growth ◽

Early Stages ◽

Brucella Suis ◽

Natural Hosts

1. Comparison of the infections of chick embryos by the chorio-allantoic route indicates that Bacterium tularense and Brucella suis, abortus, and melitensis exhibit varying degrees of facultative intracellular parasitism. Pasteurella pestis is adapted to rapid proliferation and spread in the intercellular fluids. 2. In the early stages of infection Bacterium tularense has a marked affinity for growth within ectodermal epithelial cells. Brucella suis and Brucella abortus differ in their selectivity for cells of mesodermal derivation and especially in their effect on vascular endothelium. The strain of Brucella melitensis studied is limited in its intracellular growth to ectodermal epithelium. 3. Many of the features characteristic of these infections in the natural hosts are reproduced in the chick embryo and its membranes. 4. The possible implications regarding the differences in behavior of these microorganisms in relation to the problem of infection and pathogenesis of these diseases are discussed.

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