Presence of High-Risk HPV mRNA in Relation to Future High-Grade Lesions among High-Risk HPV DNA Positive Women with Minor Cytological Abnormalities

CONTEXT: Human papillomavirus (HPV) viral load may have an important role in predicting high-grade cervical intraepithelial neoplasia (CIN) in women with cervical smears showing atypical squamous cells or LSIL. OBJECTIVE: To determine whether the assessment of the viral load of high-risk HPV DNA is useful in predicting the detection of high-grade cervical intraepithelial neoplasia (CIN2 and 3) in women referred because of cervical smears showing only atypical squamous cells or LSIL. TYPE OF STUDY: Cross-sectional SETTING: Colposcopy Clinic in a University hospital. METHODS: A series of 119 women referred because of atypical squamous cells or LSIL between August 2000 and April 2001 were included. All women were subjected to a new cervical smear, HPV testing for the high-risk types using hybrid capture II (HCII), viral load measurement in relative light units (RLU) and colposcopy, with cervical biopsies (n = 97). Cervical lesions were graded using the CIN classification. RESULTS: Cervical biopsies revealed CIN2 or CIN3 in 11% of the cases, equally among women referred because of atypical squamous cells or LSIL. The HCII test was positive in 16% of women with atypical squamous cells and 52% of those with LSIL (OR = 5.8; 95% CI 1.4 to 26.7). There was strong correlation between CIN2 or CIN3 and positivity for HPV DNA when this group was compared with women with only CIN1 or normal cervix (OR = 7.8; 95% CI 1.5 to 53.4). In ROC analysis for HCII in diagnosing CIN2 and CIN3, the area under the ROC curve was 0.784, and the viral load cutoff point of 10.0 RLU/cutoff presented 77% sensitivity and 73% specificity. Second cytology showing at least atypical squamous cells did not accurately detect CIN2 or CIN3 (OR = 6.4; 95% CI 1.0 to 50.9). The sensitivities of the second cervical smear and HCII were similar, although the specificity of HCII was significantly higher than the second cervical smear. CONCLUSIONS: The viral load of high-risk HPV types was significantly associated with the diagnosis of CIN2 or CIN3 in women referred because of atypical squamous cells and LSIL abnormalities in their cervical smear.

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Cigarette smoking and high-risk HPV DNA as predisposing factors for high-grade cervical intraepithelial neoplasia (CIN) in young Brazilian women

Acta Obstetricia Et Gynecologica Scandinavica ◽

10.1034/j.1600-0412.1998.770617.x ◽

1998 ◽

Vol 77 (6) ◽

pp. 678-682 ◽

Cited By ~ 21

Author(s):

Cecilia Maria Roteli-Martins ◽

Kazue Panetta ◽

Venancio Avancini Ferreira ALVES ◽

Sheila Aparecida Coelho Siqueira ◽

Kari Juhani Syrjänen ◽

...

Keyword(s):

High Risk ◽

Cigarette Smoking ◽

Cervical Intraepithelial Neoplasia ◽

Intraepithelial Neoplasia ◽

Predisposing Factors ◽

High Grade ◽

Hpv Dna ◽

High Risk Hpv

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HPV16 Load Is a Potential Biomarker to Predict Risk of High-Grade Cervical Lesions in High-Risk HPV-Infected Women: A Large Longitudinal French Hospital-Based Cohort Study

Cancers ◽

10.3390/cancers13164149 ◽

2021 ◽

Vol 13 (16) ◽

pp. 4149

Author(s):

Antoine Baumann ◽

Julie Henriques ◽

Zohair Selmani ◽

Aurélia Meurisse ◽

Quentin Lepiller ◽

...

Keyword(s):

Viral Load ◽

High Risk ◽

Precancerous Lesions ◽

Prognostic Score ◽

High Grade ◽

High Grade Lesion ◽

Potential Biomarker ◽

Increased Risk ◽

Cytological Abnormalities ◽

High Risk Hpv

High-risk HPV (hrHPV) testing has been implemented as a primary screening tool for cervical cancer in numerous countries. However, there is still a need for relevant triage strategies to manage hrHPV positive women to avoid excessive referral to colposcopy. The objective of this study was to assess, in women infected by hrHPV and presenting no or mild cytological abnormalities, HPV16 and HPV18 viral loads to predict the development of cervical high-grade lesion. Among 2102 women positive for hrHPV, 885 had no lesion or mild cytological abnormalities at baseline and had at least one follow-up (FU) visit. HPV16 and HPV18 prevalence was 25.9% and 8.4%, respectively. Of those women, 15% developed a high-grade lesion during the FU. An HPV16 viral load cut-off set at 3.2 log10GE/103 cells permitted to identify a subgroup of women at high risk of developing high-grade cervical lesion (HR = 2.67; 95% CI 1.80–3.97; p ≤ 0.0001). No specific HPV18 viral load threshold could have been defined in regard to the present study. In multivariate analysis, HPV16 load (absence/log10GE/103 cells < 3.2 vs. ≥3.2), RLU/PC 239 (1–100 pg/mL vs. >100 pg/mL) and cytology (normal vs abnormal) were independently associated with a significant increased risk of high-grade lesion development and were used to construct the prognostic score. In conclusion, HPV16 load is a relevant biomarker to identify women at high risk for developing cervical precancerous lesions.

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Association of human papillomavirus and bacterial vaginosis with increased risk of high-grade squamous intraepithelial cervical lesions

International Journal of Gynecological Cancer ◽

10.1136/ijgc-2018-000076 ◽

2019 ◽

Vol 29 (2) ◽

pp. 242-249 ◽

Cited By ~ 6

Author(s):

Tamy Tiemi Suehiro ◽

Natália Malaguti ◽

Edilson Damke ◽

Nelson Shozo Uchimura ◽

Fabrícia Gimenes ◽

...

Keyword(s):

Cervical Cancer ◽

High Risk ◽

Bacterial Vaginosis ◽

Low Grade ◽

High Grade ◽

Squamous Intraepithelial Lesions ◽

Hpv Dna ◽

Increased Risk ◽

High Risk Hpv ◽

Intraepithelial Lesions

ObjectiveTo assess the rates of co-infections between human papillomavirus (HPV) and 13 key markers of bacterial vaginosis in cervical samples by multiplex polymerase chain reaction in a population with a high rate of abnormal cytology and a positive HPV test.MethodsThe study included a total of 213 women aged 18–72 years screened using Papanicolaou smears for determining cervical abnormalities and for HPV and bacterial vaginosis by single-target and multiplex polymerase chain reaction.ResultsA total of 83 (39%) women were negative for intraepithelial lesion or malignancy cytology and 130 (61%) had abnormal cytology. HPV-DNA prevalence was 69.9% and bacterial vaginosis was 72.7 %. Co-infections between bacterial vaginosis with HPV-DNA and high-risk HPV were associated with an increased risk for squamous intraepithelial lesions of low-grade cytology and high-grade squamous intraepithelial lesions plus cervical cancer. The most frequent bacterial vaginosis agent was Gardnerella vaginalis (33.8%), and co-infection with HPV-DNA and high-risk HPV increased the risk for squamous intraepithelial lesions of low grade cytology and high-grade squamous intraepithelial lesions plus cervical cancer. Co-infection between Megasphaera type I and high-risk HPV increased the risk for high-grade squamous intraepithelial lesions plus cervical cancer.ConclusionsOur results reinforce the hypothesis that some bacterial vaginosis agents may play a role as co-factors in HPV-mediated cervical carcinogenesis, at least in some populations.

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Comparison of Human Papillomavirus (HPV) detection in urine and cervical samples using high-risk HPV DNA testing in Northern Thailand

10.26226/morressier.578f37fad462b8028d88f8f5 ◽

2016 ◽

Author(s):

Surapan Khunamornpong

Keyword(s):

Human Papillomavirus ◽

High Risk ◽

Dna Testing ◽

Northern Thailand ◽

Hpv Dna ◽

Hpv Dna Testing ◽

High Risk Hpv

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BORDERLINE HIGH RISK-HPV CYTOLOGY: IS OUR BIOPSY RATE SUFFICIENT TO DETECT HIGH-GRADE CIN?

10.26226/morressier.597eedbad462b80296ca0dea ◽

2017 ◽

Author(s):

Nicolò Bizzarri ◽

Nicolò Bizzarri

Keyword(s):

High Risk ◽

High Grade ◽

High Risk Hpv ◽

Biopsy Rate

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High-risk HPV-positive and -negative high-grade cervical dysplasia: Analysis of 5-year outcomes

Gynecologic Oncology ◽

10.1016/j.ygyno.2021.01.020 ◽

2021 ◽

Author(s):

Giorgio Bogani ◽

Francesco Sopracordevole ◽

Violante Di Donato ◽

Andrea Ciavattini ◽

Alessandro Ghelardi ◽

...

Keyword(s):

High Risk ◽

Cervical Dysplasia ◽

High Grade ◽

High Risk Hpv

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Comparison of Abbott RealTime High Risk HPV and Hybrid Capture 2 for the detection of high-risk HPV DNA in a referral population setting

Journal of Clinical Virology ◽

10.1016/j.jcv.2011.10.016 ◽

2012 ◽

Vol 53 (2) ◽

pp. 121-124 ◽

Cited By ~ 10

Author(s):

Simona Venturoli ◽

Elisa Leo ◽

Martina Nocera ◽

Daniela Barbieri ◽

Monica Cricca ◽

...

Keyword(s):

High Risk ◽

Hybrid Capture ◽

Hpv Dna ◽

Hybrid Capture 2 ◽

High Risk Hpv

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Relevance of the Pap Test: A Report of HPV-DNA Test-Negative High-Grade Squamous Intraepithelial Lesions of the Female Lower Genital Tract

Acta Cytologica ◽

10.1159/000448470 ◽

2016 ◽

Vol 60 (5) ◽

pp. 445-450 ◽

Cited By ~ 3

Author(s):

Yiang Hui ◽

Katrine Hansen ◽

Jayasimha Murthy ◽

Danielle Chau ◽

C. James Sung ◽

...

Keyword(s):

High Risk ◽

Pap Test ◽

Human Papillomaviruses ◽

High Grade ◽

Squamous Intraepithelial Lesions ◽

Cytologic Examination ◽

Dna Test ◽

Hpv Dna ◽

Intraepithelial Lesions ◽

Hpv Dna Test

Objective: A vast majority of cervicovaginal intraepithelial lesions are caused by high-risk human papillomaviruses (HPVs). The Pap test has been the sole method used for the screening of cervicovaginal squamous intraepithelial lesions (SIL). Recently, the FDA approved an HPV-DNA assay as a method of primary screening. We report on a series of FDA-approved HPV-DNA test-negative SIL with HPV genotyping, using an alternative method on the corresponding surgical biopsy specimens. Study Design: A retrospective review identified cytology-positive HPV-negative cases over a 15-month period at a tertiary care gynecologic oncology institution. Corresponding biopsies were reviewed and genotyped for high-risk HPVs. Results: Of the 18,200 total cases, 17 patients meeting the study criteria were selected with 27 surgical specimens corresponding to their cytologic diagnoses. Four patients with high-grade lesions were identified, 3 of whom (75%) were positive for HPV. One of these 4 patients (25%) showed high-grade SIL on biopsies from 4 separate sites in the cervix and vagina. Multiviral HPV infections were frequent. Conclusions: We discuss the relevance of cotesting for screening cervical SILs and emphasize that false-negative results are possible with the FDA-approved HPV screening assay, also in patients with high-grade SIL. These cases may be detectable by cytologic examination and this suggests that the Pap test remains an important diagnostic tool.

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