scholarly journals Comparative Metagenomic Profiling of Symbiotic Bacterial Communities Associated with Ixodes persulcatus, Ixodes pavlovskyi and Dermacentor reticulatus Ticks

PLoS ONE ◽  
2015 ◽  
Vol 10 (7) ◽  
pp. e0131413 ◽  
Author(s):  
Alexander Kurilshikov ◽  
Natalya N. Livanova ◽  
Nataliya V. Fomenko ◽  
Alexey E. Tupikin ◽  
Vera A. Rar ◽  
...  
2021 ◽  
pp. 156-160
Author(s):  
Glazunov

At present, the ixodofauna of the Tyumen region is represented by six species of ixodid ticks – these are Ixodes persulcatus Schulze, 1930; Dermacentor reticulatus Fabricius, 1794 (D. pictus, Hermann, 1804); D. marginatus Sulz, 1776; I. apronophorus P. Sch., 1924; I. (Exopalpiger) trianguliceps Bir., 1895 and I. (Ceratixodes) plumbeus, Kirsch., 1936. Three species of ixodid ticks parasitize cattle: I. persulcatus, D. reticulatus, D. marginatus. The remaining three species of ticks, Ixodes apronophorus, Ixodes strianguliceps, and Ixodes plumbeus, belong to the nest-like parasitic type and therefore are less dangerous parasites for large mammals. The most widespread ticks of the genus Ixodes and Dermacentor in the context of all natural-geographical zones of the region are ticks of the genus Dermacentor, their dominance index was 54.8%. As a result of observation, it was noted that the temperature and humidity of the air affect the activity of ixodid ticks of the genus Dermacentor in nature. Thus, the peak of ixodid activity in the spring period fell on days when the air temperature reached values from 16 oC to 26 oC, and in autumn from 15 oC to 21 oC, while lower and higher humidity indices had a depressing effect on ixodid mites of the genus Dermacentor.


2019 ◽  
Vol 12 (1) ◽  
Author(s):  
Lene Jung Kjær ◽  
Arnulf Soleng ◽  
Kristin Skarsfjord Edgar ◽  
Heidi Elisabeth H. Lindstedt ◽  
Katrine Mørk Paulsen ◽  
...  

2020 ◽  
Vol 14 (2) ◽  
pp. 38-45
Author(s):  
I. A. Novoselova ◽  
E. A. Bessolitsyna

The purpose of the research is identification of the genetic material of microorganisms of the group Borrelia burgdorferi sensu lato, of the genera Anaplasma and Ehrlichia in ixodic ticks of various species collected in the Kirov Region from 2010 to 2017. Materials and methods. Ixodic ticks were collected from the vegetation cover, people's clothing and the hair of domestic animals (dogs and cats). Species and gender of ticks were identified using definition tables. Total nucleic acids were isolated from ixodic ticks fixed in 70 % ethanol by the method using guanidine thioisocyanate buffer. Ticks’ infection by pathogens was determined using polymerase chain reaction. Results and discussion. Three species of ixodic ticks Ixodes persulcatus, Dermacentor reticulatus and I. ricinus were studied. The species I. persulcatus had the highest infection rates with the studied pathogens. The largest percentage of ticks tested was infected with Ehrlichia (35.6%). The most common were cases of simultaneous infection of ticks with Borrelia and Ehrlichia (16.3%). Cases of combined infection with three pathogens at once (Borrelia, Anaplasma and Ehrlichia) were found in all studied tick species with the same probability. Peaks of tick infection were revealed: the maximum level for all studied pathogens during the research period was noted in 2011; an increase in the number of infected ticks was observed in 2015 and 2016. 


Author(s):  
A. Ya. Nikitin ◽  
Yu. V. Sabitova ◽  
V. A. Rar ◽  
I. М. Morozov ◽  
N. S. Gordeiko ◽  
...  

The incidence of Ixodes tick-borne borreliosis (ITBB) at the Island Russky (Primorsk Territory) is higher than at the mainland. There is a bidominant structure of the tick population with mass occurrence of Ixodes persulcatus and I. pavlovskyi at the island. Due to the higher infectivity and aggressiveness of I. persulcatus, it is suggested that it plays a key role in high activity of the ITBB focus at the island.The aim of the study: to characterize the role of I. pavlovskyi in the borreliosis epizootic process based on the study of the structure of the borrelia genotype complex isolated from Ixodes ticks at the Island Russky.Materials and methods. The ticks were collected from vegetation at three sites. Ixodide species were determined morphologically and using PCR for mitochondrial gene cox1 and nuclear multicopy locus ITS2. Infection of 233 ticks was analyzed with the species identification confirmed by all methods. Borrelia genospecies were determined by multiplex two-round PCR with the primers specific to the gene fragments 5S and 23S rRNA of Borrelia burgdorferi sensu lato complex.Results and discussion. Coexistence of I. persulcatus and I. pavlovskyi at the island was confirmed, with different ratios in space and a gradual increase in the proportion of the latter species. The circulation of three etiological agents of ITBB: Borrelia garinii (predominantly in I. pavlovskyi), B. bavariensis and B. afzelii (in I. persulcatus) was identified. Thus, an active natural focus of ITBB has formed at the island, probably including two uninsulated chains of borrelia circulation. One chain combines I. persulcatus and small and large mammals as the tick feeders. The second – I. pavlovskyi that can feed on mammals and birds. Due to the spatial variability of the ixodide population structure at different sections of the island, the ratio of the circulating borrelia species can change, and this species becomes the main vector of the pathogenic agents in areas of I. pavlovskyi absolute dominance.


2015 ◽  
Vol 20 (4) ◽  
pp. 26-34
Author(s):  
V. N Bakhvalova ◽  
G. S Chicherina ◽  
V. V Panov ◽  
V. V Glupov ◽  
O. V Morozova

A comparative analysis of the virus infection carrier state and distribution of genetic types of the virus of tick-borne encephalitis (TBE) among invertebrates (2 species of ticks, Ixodes persulcatus Schulze and Ixodes pavlovskyi Pomerantsev 1946) and vertebrate reservoir hosts (4 species of small rodents: the red vole Myodes rutilus Pallas, gray-sided vole Myodes rufocanus Sundevall, field mouse Apodemus agrarius Pallas, birch mice Sicista betulina Pallas and 1 species of insectivores - common shrew Sorex araneus L (1758)), dominating on the territory of the Novosibirsk region in 2009-14 years, was performed with the use of virological and molecular biological methods. Frequency detection of RNA and / or E protein in mammals (70,9 ± 3,0%) were shown to considerably exceed levels of virus infection carrier state rate of ticks (3,4 ± 0,4). In the circulation of three major types of TBE - Far East (FE), Siberian (NIB) and European (Eur) in natural populations in mono - or polytype forms in mites Sib type prevailed (p


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