scholarly journals Characterisation of morphological differences in well-differentiated nasal epithelial cell cultures from preterm and term infants at birth and one-year

PLoS ONE ◽  
2018 ◽  
Vol 13 (12) ◽  
pp. e0201328 ◽  
Author(s):  
Helen E. Groves ◽  
Hong Guo-Parke ◽  
Lindsay Broadbent ◽  
Michael D. Shields ◽  
Ultan F. Power
Keyword(s):  
Epithelial Cell ◽  
Cell Cultures ◽  
Term Infants ◽  
Nasal Epithelial Cell ◽  
Epithelial Cell Cultures ◽  
Well Differentiated ◽  
Morphological Differences ◽  
One Year

Well-Differentiated Human Airway Epithelial Cell Cultures

2004 ◽  
pp. 183-206 ◽  
Author(s):  
M. Leslie Fulcher ◽  
Sherif Gabriel ◽  
Kimberlie A. Burns ◽  
James R. Yankaskas ◽  
Scott H. Randell
Keyword(s):  
Epithelial Cell ◽  
Cell Cultures ◽  
Airway Epithelial Cell ◽  
Airway Epithelial ◽  
Epithelial Cell Cultures ◽  
Human Airway ◽  
Human Airway Epithelial Cell ◽  
Well Differentiated

scholarly journals Identification of H3N2 NA and PB1-F2 genetic variants and their association with disease symptoms during the 2014-15 influenza season

2020 ◽  
Author(s):  
Deena R. Blumenkrantz ◽  
Thomas Mehoke ◽  
Kathryn Shaw-Saliba ◽  
Harrison Powell ◽  
Nicholas Wohlgemuth ◽  
...  
Keyword(s):  
Epithelial Cell ◽  
Cell Cultures ◽  
Influenza A ◽  
Influenza Season ◽  
Glycosylation Site ◽  
Antigenic Drift ◽  
Parental Genotype ◽  
Nasal Epithelial Cell ◽  
Human Nasal Epithelial Cell ◽  
Epithelial Cell Cultures

AbstractThe 2014-15 influenza season saw the emergence of an H3N2 antigenic drift variant that formed the 3C.2a HA clade. Whole viral genomes were sequenced from nasopharyngeal swabs of 94 patients with confirmed influenza A virus infection and primary human nasal epithelial cell cultures used to efficiently isolate H3N2 viruses. The isolates were classified by HA clade and the presence of a new set of co-selected mutations in NA (a glycosylation site, NAg+) and PB1-F2 (H75P). The NA and PB1-F2 mutations were present in a subset of clade 3C.2a viruses (NAg+F2P) which dominated during the subsequent influenza seasons. In human nasal epithelial cell cultures, a virus with the novel NAg+F2P genotype replicated less well compared to a virus with the parental genotype. Retrospective analyses of clinical data showed that NAg+F2P genotype viruses were associated with increased cough and shortness of breath in infected patients.

scholarly journals Characterisation of morphological differences in well-differentiated nasal epithelial cell cultures from preterm and term infants at birth and one-year

2018 ◽  
Author(s):  
Helen E. Groves ◽  
Hong Guo-Parke ◽  
Lindsay Broadbent ◽  
Michael D. Shields ◽  
Ultan F. Power
Keyword(s):  
Epithelial Cell ◽  
Immune Responses ◽  
Airway Epithelium ◽  
Newborn Infants ◽  
Innate Immune ◽  
Innate Immune Responses ◽  
Nasal Epithelial Cell ◽  
Age Related ◽  
Well Differentiated ◽  
One Year

AbstractBackgroundInnate immune responses of airway epithelium are important defences against respiratory pathogens and allergens. Newborn infants are at greater risk of severe respiratory infections compared to older infants. However, very little is known regarding human neonatal airway epithelium immune responses and whether age-related morphological and/or innate immune changes contribute to the development of airway disease.MethodsWe collected nasal epithelial cells from 41 newborn infants (23 term, 18 preterm) within 5 days of birth. Repeat sampling was achieved for 24 infants (13 term, 11 preterm) at a median age of 12.5 months. Morphologically and physiologically authentic well-differentiated primary paediatric nasal epithelial cell (WD-PNEC) cultures were generated and characterised using light microscopy and immunofluorescence.ResultsWD-PNEC cultures were established for 15/23 (65%) term and 13/18 (72%) preterm samples at birth, and 9/13 (69%) term and 8/11 (73%) preterm samples at one-year. Newborn and infant WD-PNEC cultures demonstrated extensive cilia coverage, mucous production and tight junction integrity. Newborn WD-PNECs took significantly longer to reach full differentiation and were noted to have much greater proportions of goblet cells compared to one-year repeat WD-PNECs. No differences were evident in ciliated/goblet cell proportions between term- and preterm-derived WD-PNECs at birth or one-year old.ConclusionWD-PNEC culture generation from newborn infants is feasible and represents a powerful and exciting opportunity to study differential innate immune responses in human airway epithelium very early in life.

CFTR Transgene Expression in Primary ΔF508 Epithelial Cell Cultures From Human Nasal Polyps Following Gene Transfer With Cationic Phosphonolipids

2004 ◽  
Vol 26 (3) ◽  
pp. 193-206 ◽  
Author(s):  
Tristan Montier ◽  
Pascal Delépine ◽  
Rémi Marianowski ◽  
Karine Le Ny ◽  
Morgane Le Bris ◽  
...  
Keyword(s):  
Gene Transfer ◽  
Epithelial Cell ◽  
Cell Cultures ◽  
Transgene Expression ◽  
Nasal Polyps ◽  
Epithelial Cell Cultures
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