scholarly journals Molecular Characterization and Demographic Study on Infectious Bursal Disease Virus in Faisalabad District

PLoS ONE ◽  
2021 ◽  
Vol 16 (8) ◽  
pp. e0254605
Author(s):  
Sanaullah Sajid ◽  
Sajjad ur Rahman ◽  
Mashkoor Mohsin Gilani ◽  
Zia ud Din Sindhu ◽  
Manel Ben Ali ◽  
...  
Keyword(s):  
Molecular Characterization ◽  
Disease Virus ◽  
Infectious Bursal Disease Virus ◽  
Nucleotide Sequences ◽  
Bursa Of Fabricius ◽  
Infectious Bursal Disease ◽  
Economic Losses ◽  
Effective Prevention ◽  
Molecular Features ◽  
Bursal Disease

The re-emergence of virulent strains of the Infectious Bursal Disease Virus (IBDV) leads to significant economic losses of poultry industry in Pakistan during last few years. This disease causes the infection of bursa, which leads to major immune losses. A total number of 30 samples from five IBD outbreaks during the period of 2019–20 were collected from different areas of Faisalabad district, Pakistan and assayed by targeting the IBD virus VP2 region through RT-PCR. Among all the outbreaks, almost 80% of poultry birds were found positive for the IBDV. The bursa tissues were collected from the infected birds and histopathological examination of samples revealed severe lymphocytic depletion, infiltration of inflammatory cells, and necrosis of the bursa of Fabricius (BF). Positive samples were subjected to re-isolation and molecular characterization of IBDV. The Pakistan IBDV genes were subjected to DNA sequencing to determine the virus nucleotide sequences. The sequences of 100 Serotype-I IBDVs showing nearest homology were compared and identified with the study sequence. The construction of the phylogenetic tree for nucleotide sequences was accomplished by the neighbor-joining method in MEGA-6 with reference strains. The VP2 segment reassortment of IBDVs carrying segment A were identified as one important type of circulating strains in Pakistan. The findings indicated the molecular features of the Pakistan IBDV strains playing a role in the evolution of new strains of the virus, which will contribute to the vaccine selection and effective prevention of the disease.

scholarly journals Chicken Heat Shock Protein 70 Is an Essential Host Protein for Infectious Bursal Disease Virus Infection In Vitro

Pathogens ◽  
2021 ◽  
Vol 10 (6) ◽  
pp. 664
Author(s):  
Yufang Meng ◽  
Xiaoxue Yu ◽  
Chunxue You ◽  
Wenjuan Zhang ◽  
Yingfeng Sun ◽  
...  
Keyword(s):  
Heat Shock ◽  
Heat Shock Protein ◽  
Disease Virus ◽  
Heat Shock Protein 70 ◽  
Infectious Bursal Disease Virus ◽  
Host Protein ◽  
Infectious Bursal Disease ◽  
Economic Losses ◽  
Small Interfering Rnas ◽  
Bursal Disease

Infectious bursal disease virus (IBDV) infection causes pathogenicity and mortality in chickens, leading to huge economic losses in the poultry industry worldwide. Studies of host-virus interaction can help us to better understand the viral pathogenicity. As a highly conservative host factor, heat shock protein 70 (Hsp70) is observed to be involved in numerous viral infections. However, there is little information about the role of chicken Hsp70 (cHsp70) in IBDV infection. In the present study, the increased expression of cHsp70 was observed during IBDV-infected DF-1 cells. Further studies revealed that Hsp70 had similar locations with the viral double-stranded RNA (dsRNA), and the result of pull-down assay showed the direct interaction between cHsp70 with dsRNA, viral proteins (vp)2 and 3, indicating that maybe cHsp70 participates in the formation of the replication and transcription complex. Furthermore, overexpression of cHsp70 promoted IBDV production and knockdown of cHsp70 using small interfering RNAs (siRNA) and reducedviral production, implying the necessity of cHsp70 in IBDV infection. These results reveal that cHsp70 is essential for IBDV infection in DF-1 cells, suggesting that targeting cHsp70 may be applied as an antiviral strategy.

Molecular characterization of infectious bursal disease virus (IBDV): Diversity of very virulent IBDV in Tanzania

2007 ◽  
Vol 152 (4) ◽  
pp. 783-790 ◽  
Author(s):  
C. J. Kasanga ◽  
T. Yamaguchi ◽  
P. N. Wambura ◽  
A. D. Maeda-Machang’u ◽  
K. Ohya ◽  
...  
Keyword(s):  
Molecular Characterization ◽  
Disease Virus ◽  
Infectious Bursal Disease Virus ◽  
Infectious Bursal Disease ◽  
Bursal Disease

scholarly journals MOLECULAR CHARACTERIZATION OF INFECTIOUS BURSAL DISEASE VIRUS FROM COMMERCIAL POULTRY IN PAKISTAN

2017 ◽  
Vol 1 (2) ◽  
pp. 01-06
Author(s):  
Rai Shafqat Ali Khan ◽  
Mudasser Habib ◽  
Muhammad Salah Ud Din Shah ◽  
Waqas Ali ◽  
Zaheer Hussain ◽  
...  
Keyword(s):  
Molecular Characterization ◽  
Disease Virus ◽  
Infectious Bursal Disease Virus ◽  
Infectious Bursal Disease ◽  
Bursal Disease ◽  
Commercial Poultry

scholarly journals Genome-wide analysis of differentially expressed mRNAs, lncRNAs, and circRNAs in chicken bursae of Fabricius during infection with very virulent infectious bursal disease virus

BMC Genomics ◽  
2020 ◽  
Vol 21 (1) ◽  
Author(s):  
Xuewei Huang ◽  
Junyan Zhang ◽  
Zengsu Liu ◽  
Meng Wang ◽  
Xiaolong Fan ◽  
...  
Keyword(s):  
Signaling Pathway ◽  
Disease Virus ◽  
Infectious Bursal Disease Virus ◽  
Expression Profiles ◽  
Circular Rna ◽  
Differentially Expressed ◽  
Bursa Of Fabricius ◽  
Infectious Bursal Disease ◽  
Non Coding Rna ◽  
Bursal Disease

Abstract Background Infectious bursal disease virus (IBDV) causes acute, highly contagious, immunosuppressive, and lethal infectious disease in young chickens and mainly infects the bursa of Fabricius (BF). To investigate interactions between IBDV and its host, RNA sequencing was applied to analyze the responses of the differentially expressed transcriptional profiles of BF infected by very virulent IBDV (vvIBDV). Results In total, 317 upregulated and 94 downregulated mRNAs were found to be significantly differentially expressed in infected chickens, compared to controls. Long non-coding RNA (lncRNA) and circular RNA (circRNA) alterations were identified in IBDV-infected chickens, and significantly different expression was observed in 272 lncRNAs and 143 circRNAs. Gene Ontology and Kyoto Encyclopedia of Genes and Genomes pathway enrichment analyses were performed to assess the functions of significantly dysregulated genes, which showed that the JAK-STAT signaling pathway, the NOD-like receptor signaling pathway, and apoptosis may be activated by IBDV infection. We predicted interactions between differentially expressed genes and produced lncRNA-mRNA and circRNA-miRNA-mRNA regulator network. Conclusions The present study identified the expression profiles of mRNAs, lncRNAs, and circRNAs during vvIBDV infection and provides new insights into the pathogenesis of IBDV and antiviral immunity of the host.

Molecular Characterization of Pakistani Field Isolates of Infectious Bursal Disease Virus

2009 ◽  
Vol 4 (2) ◽  
pp. e16-e16
Author(s):  
Nazir Ahmed Lone ◽  
Shafqat Fatima Rehmani ◽  
Shahana Urooj Kazmi ◽  
Rana Muzaffar ◽  
Taseer Ahmed Khan ◽  
...  
Keyword(s):  
Molecular Characterization ◽  
Disease Virus ◽  
Infectious Bursal Disease Virus ◽  
Infectious Bursal Disease ◽  
Field Isolates ◽  
Bursal Disease

scholarly journals Pathogenicity and Effects on Lymphoid Organs of Recent Moroccan Very Virulent Infectious Bursal Disease Virus in Broilers and SPF Chickens

2021 ◽  
Author(s):  
Charifa DRISSI TOUZANI ◽  
Imane MAAROUFI ◽  
Siham FELLAHI ◽  
Ikhlass EL BERBRI ◽  
Fatima-zohra SIKHT ◽  
...  
Keyword(s):  
Disease Virus ◽  
Infectious Bursal Disease Virus ◽  
Clinical Signs ◽  
Total Mortality ◽  
Bursa Of Fabricius ◽  
Infectious Bursal Disease ◽  
Control Group ◽  
Bursal Disease ◽  
Specific Pathogen ◽  
And Control

Abstract The aim of the current study is to evaluate the pathogenicity of recent infectious bursal disease virus (IBDV) (1/chicken/Morocco/IB19/2017) genetically characterized as vvIBDV belonging to genogroup 3.Two chicken lines, broiler and specific-pathogen-free (SPF) chickens, were inoculated by occulonasal route with 0.2 ml of the 105EID50 /ml of viral solution of IB19 vvIBDV strain at 29 days of age. The experimental monitoring was carried out during 10 days post challenge (dpc). The clinical signs stared on day 2 pc with maximum severity observed between 3 and 6 dpc. The total mortality rate reached 10% in broilers (group G1) and 93% in SPF (G3). The macroscopic lesions in broilers G1 was a marked hypertrophy of the bursa of Fabricius (BF) with slight haemorrhage observed between 2 to 4 dpc, followed by very pronounced atrophy observed on the 5 dpc. The post-mortem examinations of dead SPF birds (G3) revealed on 3 dpc very haemorrhagic BF with black cherry appearance in 80 % of dead birds. The mean Bursa/Body Index (BBI) of challenged broilers (G1) showed a decrease of 46% on day 9 pc compared to broilers control group (G2) indicating bursal atrophy. The microscopic lesions found in the BF on 3 dpc consisted mainly of inflammation with severe lymphoid depletion of the follicles. The evaluation of recent vvIBDV outbreak is very important to understand its epidemiology and will contribute to the efficient prevention and control of IBD.

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