Hybridization of Horseradish Peroxidase-Labeled Probes and Detection by Enhanced Chemiluminescence

2003 ◽  
pp. 127-134
Author(s):  
Timothy Stone ◽  
Ian Durrant
Keyword(s):  
Horseradish Peroxidase ◽  
Enhanced Chemiluminescence

Flow-Injection Enzyme Immunoassay for Human IgG by Using Enhanced Chemiluminescence Reaction for Horseradish Peroxidase Label Quantitation

1989 ◽  
Vol 22 (8) ◽  
pp. 1841-1859 ◽  
Author(s):  
A. P. Osipov ◽  
A. A. Arefyev ◽  
S. B. Vlasenko ◽  
E. M. Gavrilova ◽  
A. M. Yegorov
Keyword(s):  
Horseradish Peroxidase ◽  
Enzyme Immunoassay ◽  
Flow Injection ◽  
Human Igg ◽  
Enhanced Chemiluminescence

scholarly journals Enhanced chemiluminescence for tissue antigen and cellular viral DNA detection.

1990 ◽  
Vol 38 (3) ◽  
pp. 415-419 ◽  
Author(s):  
E Hawkins ◽  
R Cumming
Keyword(s):  
Horseradish Peroxidase ◽  
Liquid Nitrogen ◽  
Dna Detection ◽  
Colorimetric Detection ◽  
Light Output ◽  
Tissue Antigen ◽  
Viral Dna ◽  
Enhanced Chemiluminescence ◽  
Tissue Antigens

The ability to use enhanced chemiluminescence (ECL) to detect horseradish peroxidase as a label for tissue antigens and cellular viral DNA was demonstrated. A liquid nitrogen-cooled charged-coupled device (CCD) was used to detect light output, which was visualized on a monitor or was quantitated using an attached microcomputer. In a tissue antigen model, equivalent sensitivity was observed between ECL and colorimetric detection.

An Enhanced Chemiluminescence Enzyme Immunoassasy for Serum Oestradiol

1988 ◽  
Vol 25 (3) ◽  
pp. 288-292 ◽  
Author(s):  
G M Sankolli ◽  
Raw Stott ◽  
G H G Thorpe ◽  
D Smith ◽  
B T Rudd ◽  
...  
Keyword(s):  
Enzyme Activity ◽  
Horseradish Peroxidase ◽  
Enzyme Immunoassay ◽  
Hydroxycinnamic Acid ◽  
Microtitre Plate ◽  
Sample Concentration ◽  
Enhanced Chemiluminescence ◽  
Chemiluminescent Reaction ◽  
Chemiluminescent Enzyme Immunoassay ◽  
Good Agreement

Oestradiol in serum was determined with a simple enhanced chemiluminescent enzyme immunoassay. The assay is based on oestradiol labelled with horseradish peroxidase and the IgG fraction of an oestradiol antiserum coated on a black polystyrene microtitre plate. The enzyme activity of bound label was determined using a p-hydroxycinnamic acid-enhanced chemiluminescent reaction. The assay was sensitive (1·8 fmol/well), precise (intra- and inter-assay CV 4–10% and 8–12%, respectively for sample concentration in the range 122–1330 pmol/L) and showed good agreement with conventional radioimmunoassays ( r=0·99).

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