Bacteriocin Typing

2003 ◽  
pp. 5-14 ◽  
Author(s):  
Tyrone L. Pitt ◽  
Michael A. Gaston
Keyword(s):  
Bacteriocin Typing

A Microbiologic Approach to the Investigation of Bacterial Nosocomial Infection Outbreaks

1980 ◽  
Vol 1 (06) ◽  
pp. 391-400 ◽  
Author(s):  
Donald A. Goldmann ◽  
Ann B. Macone
Keyword(s):  
Nosocomial Infection ◽  
Susceptibility Testing ◽  
Resistant Bacteria ◽  
Antibiotic Susceptibility Testing ◽  
Bacterial Isolates ◽  
Infection Control Team ◽  
Antibiotic Resistant ◽  
Plasmid Analysis ◽  
Identification Of Bacteria ◽  
Bacteriocin Typing

This article details the appropriate microbiologic support that is critical to the successful investigation of nosocomial infection problems. The infection control team must have ready access to microbiologic data, and the laboratory should retain epidemiologically relevant bacterial isolates. Investigation of epidemics is facilitated by precise identification of bacteria and careful antibiotic susceptibility testing. In some situations, biotyping, serotyping, phage typing, bacteriocin typing, and other specialized techniques may be required. Plasmid analysis may be useful in the investigation of nosocomial infection problems caused by antibiotic-resistant bacteria.

Bacteriocin Typing of Vibrio cholerae

1970 ◽  
Vol 1 (3) ◽  
pp. 293-299
Author(s):  
A. N. Chakrabarty ◽  
Sati Adhya ◽  
Jayantisri Basu ◽  
Sujata G. Dastidar
Keyword(s):  
Vibrio Cholerae ◽  
Cold Shock ◽  
Casein Hydrolysate ◽  
Bacteriocin Production ◽  
Buffer Concentration ◽  
Indicator Organisms ◽  
Proteose Peptone ◽  
Bacteriocin Typing ◽  
Citrate Phosphate ◽  
Citrate Phosphate Buffer

Bacteriocins of Vibrio cholerae have been demonstrated against enterobacterial and vibrio indicator organisms by conventional techniques. Abundant bacteriocin production took place on casein hydrolysate-yeast extract, tryptic soy, digest broth, proteose-peptone, and neopeptone agars. Essential factors were a citrate-phosphate buffer concentration of 0.5 to 0.7%, at p H 7.5 to 7.6, and cold shock. Thermal treatment of indicator organisms at 45 C for 12 min increased the percentage of typable strains. The bacteriocins of V. cholerae appeared to be powerful diffusible bactericidal agents. By using 8 indicator strains, 11 bacteriocin types have been recognized among 425 strains, of which 87% are typable at present.

scholarly journals The assessment and application of a bacteriocin typing scheme forClostridium perfringens

1985 ◽  
Vol 94 (1) ◽  
pp. 69-79 ◽  
Author(s):  
G. N. Watson
Keyword(s):  
Clostridium Perfringens ◽  
Food Poisoning ◽  
Computer Programs ◽  
Typing Scheme ◽  
Wide Range ◽  
Bacteriocin Typing ◽  
Different Sources ◽  
Susceptibility Patterns

SUMMARYA collection of 50 bacteriocins was assembled and used to type 802 isolates ofClostridium perfringensfrom food poisoning outbreaks and a variety of other sources. It was found that strains of the same serotype within an outbreak showed similar patterns of susceptibility to bacteriocins, and the use of a ‘one difference’ rule is proposed for interpretation of the typing patterns of epidemiologically related strains. Isolates of different serotype or of the same serotype isolated from different sources produced many variations in bacteriocin susceptibility patterns.Two computer programs were developed to assist in the interpretation of bacteriocin typing patterns. Their use showed that related and unrelated strains formed different clusters and enabled a range of the 20 most discriminatory bacteriocins to be selected.Isolates ofC. perfringensfrom a wide range of sources were screened for their ability to produce bacteriocins. A much greater proportion of the strains from food poisoning outbreaks was bacteriocinogenic than were isolates from human and animal infections, various foods and the environment. The relevance of these findings to the occurrence ofC. perfringensfood poisoning is discussed.

Reproducibility of three different methods for bacteriocin typing of Klebsiella species

1983 ◽  
Vol 49 (2) ◽  
pp. 206-206
Author(s):  
A. M. Simoons-Smit ◽  
A. M. J. J. Verwey-Van Vught ◽  
I. Y. R. Kanis ◽  
D. M. MacLaren
Keyword(s):  
Klebsiella Species ◽  
Bacteriocin Typing

scholarly journals Serratia marcescens infection associated with early abortion in cows and buffaloes

1988 ◽  
Vol 101 (1) ◽  
pp. 143-149 ◽  
Author(s):  
A. M. Das ◽  
V. L. Paranjape ◽  
T. L. Pitt
Keyword(s):  
Serratia Marcescens ◽  
Pure Culture ◽  
Vaginal Discharge ◽  
Cervical Mucus ◽  
Internal Organs ◽  
Serotype O ◽  
Bacteriocin Typing ◽  
Different Strains ◽  
Early Abortion ◽  
Septic Abortion

SUMMARYSerratia marcescens was isolated in pure culture from cases of septic abortion in 4 cows on one farm and 10 buffaloes on two other farms. A reddish vaginal discharge was observed after abortion in all animals and in the internal organs of the aborted fetuses. All but two of the isolates produced prodigiosin, and two of the isolates from buffaloes were atypical in that they fermented raffinose. O-serological, bacteriophage and bacteriocin typing revealed four different strains. All cows were infected by the same strain, and this strain was also isolated from the semen of a breeding bull on the same farm. In another farm a strain of serotype O 14 was isolated from 6 of 10 buffaloes, and two other distinct strains were isolated from the remainder. The strain from the cattle was sensitive to gentamicin and so were two of the buffalo isolates. The infected cows were treated with intra-uterine gentamicin and the organism disappeared from cervical mucus after 3 days. Each animal after abortion showed a raised titre of agglutinating antibody to their respective isolate. A survey of 1172 healthy buffaloes and cattle gave an incidence of 1·8% with raised titres towards S. marcescens.

scholarly journals Distribution of Clinical Isolates of Serratia marcescens by Bacteriocin Typing

1984 ◽  
Vol 58 (4) ◽  
pp. 312-317
Author(s):  
Masaru NASU ◽  
Jun GOTO ◽  
Yoichiro GOTO ◽  
Takayoshi TASHIRO ◽  
Takashi ITOGA ◽  
...  
Keyword(s):  
Serratia Marcescens ◽  
Clinical Isolates ◽  
Bacteriocin Typing

scholarly journals The development and assessment of a bacteriocin typing method forKlebsiella

1979 ◽  
Vol 82 (2) ◽  
pp. 207-223 ◽  
Author(s):  
A. S. Edmondson ◽  
E. Mary Cooke
Keyword(s):  
Close Agreement ◽  
Reliable Method ◽  
Natural Populations ◽  
Good Discrimination ◽  
Typing Method ◽  
Acceptable Accuracy ◽  
Cultural Conditions ◽  
Typing Methods ◽  
Complete Set ◽  
Bacteriocin Typing

SUMMARYKlebsiellas are generally typed by the method of capsular serotyping but, although this is a reliable method, it is time consuming, requires the production of a large number of antisera and is not generally available. For this reason another method for typing klebsiellas was sought.A bacteriocin typing method involving mitomycin C induction was developed and the cultural conditions giving optimum klebecin production and the best methods of testing the sensitivity of the organisms to klebecins were determined.Of 190 klebsiella strains screened for bacteriocinogeny, only 68 (35·8%) produced klebecin and after calculation of similarity values by computer analysis, a typing set of 15 producers was selected. This typing set allowed over 96% of klebsiella strains to be typed and tests of the reproducibility of the method and the variability of typing patterns in natural populations of klebsiella indicated that results of acceptable accuracy could be obtained, while retaining good discrimination if two or more differences were required between patterns before they were regarded as distinct.A complete set of capsular antisera were prepared, enabling the results obtained from klebecin typing to be compared with those from serotyping. There was generally close agreement between the results from the two typing methods and greater discrimination was obtained between similar strains when the two methods were combined. Klebecin typing and serotyping revealed relationships between strains from five outbreaks of infection, and strains of the same serotype from different hospitals could frequently be distinguished by their klebecin typing patterns.

scholarly journals Bacteriocin typing of Clostridium perfringens in human feces

1978 ◽  
Vol 7 (3) ◽  
pp. 307-309
Author(s):  
D E Mahony ◽  
C A Swantee
Keyword(s):  
Clostridium Perfringens ◽  
Human Feces ◽  
Stool Specimens ◽  
Bacteriocin Typing

Three hundred and ninety-nine isolates of Clostridium perfringens from enriched stool specimens of 51 individuals (about eight colonies per person) were typed by bacteriocins. Forty-nine percent of these persons carried more than one bacteriocin type in their stool, and some had three or four different stains as determined by bacteriocin typing. Weekly stool specimens obtained from seven positive volunteers over a period of 5 weeks were screened for C. perfringens, and several colonies from each person were typed. This survey demonstrated that the number of types fluctuated with time, several types could be carried simultaneously, and the isolation of the organism was variable. Nine new bacteriocin types of C. perfringens were isolated in this study.

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