Primary Rat Mesencephalic Neuron-Glia, Neuron-Enriched, Microglia-Enriched, and Astroglia-Enriched Cultures

SUMMARYEscherichia coli, a prominent waterborne pathogen, causes a variety of gastrointestinal and extraintestinal infections that depend on virulence determinants. To monitor natural aquatic systems for virulence-associated genes ofE. coli, multiplex PCR was used in a survey covering 46 major natural water bodies in Bangladesh. DNA was extracted directly from water samples as well as from pre-enriched and enriched cultures during three successive seasons and assessed forE. colivirulotype distribution. From the five virulotypes, genes from the enterotoxigenic (ETEC), enteropathogenic (EPEC), and enterohaemorrhagic (EHEC) virulotypes were detected consistently, but genes from the enteroinvasive (EIEC) and enteroaggregative (EAEC) virulotypes were traced only occasionally. ETEC was the most prevalent virulotype, followed by EPEC. However, EIEC and EAEC virulotypes could not be detected in winter or the rainy season, respectively. Specific regional distribution patterns of differentE. colivirulotypes and their temporal fluctuations were identified. These observations may assist with assessing seasonal risk and identifying vulnerable areas of the country prone toE. coli-associated outbreaks.

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Screening and Isolation of Bacterial Strains with the Biotechnological Potential to Destruct Cyanide-Bearing Compounds under the Conditions of Extreme Continental Climate

Advanced Materials Research ◽

10.4028/www.scientific.net/amr.1130.19 ◽

2015 ◽

Vol 1130 ◽

pp. 19-22

Author(s):

M.P. Belykh ◽

S.V. Petrov ◽

V.F. Petrov ◽

A.Yu. Chikin ◽

N.L. Belkova

Keyword(s):

Microbial Communities ◽

Gold Mining ◽

Molecular Genetic ◽

Heap Leaching ◽

Bacterial Strains ◽

Mining Operations ◽

Biotechnological Potential ◽

Enriched Cultures ◽

Continental Climate

The methods of biodegradation are of special interest because they help solving environmental problems of wastes detoxification from gold-mining operations. The use of bacterial strains is a promising approach in the field of biotechnology to destruct cyanide-bearing compounds. The diversity of microbial communities both in heap in situ and in the enriched cultures was studied with molecular genetic methods. The differences in representation of bacteria, cultivated in unexploitable and operating heaps, are territory, site and heap specific. The strains of Pseudomonas sp. and Methylobacterium sp. possess the biotechnological potential and might be used in biodegradation of heap leaching wastes in extreme continental climate.

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Parathyroid Hormone Enhances the Transcript and Polypeptide Levels of Insulin-Like Growth Factor I in Osteoblast-Enriched Cultures from Fetal Rat Bone*

Endocrinology ◽

10.1210/endo-124-3-1247 ◽

1989 ◽

Vol 124 (3) ◽

pp. 1247-1253 ◽

Cited By ~ 242

Author(s):

THOMAS L. MCCARTHY ◽

MICHAEL CENTRELLA ◽

ERNESTO CANALIS

Keyword(s):

Parathyroid Hormone ◽

Growth Factor ◽

Insulin Like Growth Factor ◽

Factor I ◽

Fetal Rat ◽

Growth Factor I ◽

Enriched Cultures ◽

Rat Bone

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Persistent Infection of Human Thymic Epithelial Cells by Coxsackievirus B4

Journal of Virology ◽

10.1128/jvi.76.10.5260-5265.2002 ◽

2002 ◽

Vol 76 (10) ◽

pp. 5260-5265 ◽

Cited By ~ 36

Author(s):

Fabienne Brilot ◽

Wassim Chehadeh ◽

Chantal Charlet-Renard ◽

Henri Martens ◽

Vincent Geenen ◽

...

Keyword(s):

Protein Detection ◽

Thymic Epithelial Cells ◽

Thymic Epithelial Cell ◽

Coxsackievirus B4 ◽

Reverse Transcription Pcr ◽

Macrophage Colony Stimulating Factor ◽

Macrophage Colony ◽

Enriched Cultures ◽

Vp1 Capsid Protein ◽

Stimulating Factor

ABSTRACT Persistent replication of coxsackievirus B4 (CVB4) E2 (diabetogenic) and CVB4 JBV (nondiabetogenic) strains in thymic epithelial cell (TEC)-enriched cultures (≥95%) was proved by detection of positive- and negative-strand viral RNA by reverse transcription-PCR in extracted RNA from cell cultures, VP1 capsid protein detection by immunofluorescence (IF) staining, and release of infectious particles up to 30 days after infection without obvious cytolysis. By double-IF staining, cytokeratin-containing cells were shown to be susceptible to CVB4. The persistence of CVB4 was associated with a significantly increased rate of TEC proliferation (up to 70%) after 20 days of culture and a significantly increased chronic production of immunoreactive interleukin-6 (IL-6), leukemia inhibitory factor, and granulocyte-macrophage colony-stimulating factor in supernatant after 3 days of culture. The CVB4 replication and the release of cytokines were not restricted to the CVB4 E2 diabetogenic strain and did not depend on the genetic background of the host; however, TEC were more responsive to CVB4 E2 than CVB4 JBV as far as the production of cytokines.

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