Improvements in Lipase Production and Recovery from Acinetobacter radioresistens in Presence of Polypropylene Powders Filled with Carbon Sources

2003 ◽  
Vol 104 (2) ◽  
pp. 129-140 ◽  
Author(s):  
In-Liang Liu ◽  
Shau-Wei Tsai
Keyword(s):  
Carbon Sources ◽  
Lipase Production ◽  
Acinetobacter Radioresistens

Lipase Production by Acinetobacter radioresistens in the Presence of a Nonwoven Fabric

1999 ◽  
Vol 15 (5) ◽  
pp. 919-922 ◽  
Author(s):  
C.-C. Shen ◽  
J.-Y. Wu ◽  
C.-Y. Chen ◽  
T.-L. Chen
Keyword(s):  
Nonwoven Fabric ◽  
Lipase Production ◽  
Acinetobacter Radioresistens

Lipase production by acinetobacter radioresistens in a batch fill- and-draw culture

1998 ◽  
Vol 73 (2-3) ◽  
pp. 185-194 ◽  
Author(s):  
Ta-Jung Wang ◽  
Teh-Liang Chen
Keyword(s):  
Lipase Production ◽  
Acinetobacter Radioresistens

The lipase activity of Talaromyces emersonii

1978 ◽  
Vol 56 (16) ◽  
pp. 1840-1843 ◽  
Author(s):  
B. A. Oso
Keyword(s):  
Liquid Medium ◽  
Yeast Extract ◽  
Lipase Activity ◽  
Natural Habitat ◽  
Carbon Sources ◽  
Lipase Production ◽  
Extracellular Lipase ◽  
Talaromyces Emersonii ◽  
Stationary Liquid ◽  
Presence And Absence

Studies were carried out on the ability of Talaromyces emersonii Stolk to produce extracellular lipase in stationary liquid medium under various conditions. The best temperatures for lipase synthesis and activity were 40–45 °C, and at all the temperatures (37–55 °C) tested for lipase production, maximum enzyme was produced 8 days after incubation. Lipase synthesis was induced when various carbohydrates were used as carbon sources both in the presence and absence of yeast extract. The significance of lipase production in relation to the natural habitat is discussed.

scholarly journals Enzymatic activity of a novel halotolerant lipase from Haloarcula hispanica 2TK2

2016 ◽  
Vol 18 (2) ◽  
pp. 20-25 ◽  
Author(s):  
Melis Ozgen ◽  
Azade Attar ◽  
Yeliz Elalmis ◽  
Meral Birbir ◽  
Sevil Yucel
Keyword(s):  
Salt Concentration ◽  
Lipolytic Activity ◽  
Carbon Sources ◽  
Lipase Production ◽  
Relative Activity ◽  
Walnut Oil ◽  
Nacl Concentration ◽  
Potential Applications ◽  
Triton X ◽  
Haloarcula Hispanica

Abstract A strain of Haloarcula hispanica isolated from Tuzkoy salt mine, Turkey exhibited extracellular lipolytic activity. Important parameters such as carbon sources and salt concentration for lipase production were investigated. Optimal conditions for the enzyme production from Haloarcula hispanica 2TK2 were determined. It was observed that the lipolytic activity of Haloarcula hispanica was stimulated by some of the carbon sources. The high lipase acitivity values were obtained in the presence of 2% (v/v) walnut oil (6.16 U/ml), 1% (v/v) fish oil (5.07 U/ml), 1% (v/v) olive oil (4.52 U/ml) and 1% (w/v) stearic acid (4.88 U/ml) at 4M NaCl concentration. Lipase was partially purified by ammonium sulfate precipitation and ultrafiltration. Optimal temperature and pH values were determined as 45°C and 8.0, respectively. Lipase activity decreased with the increasing salt concentration, but 85% activity of the enzyme was maintained at 5M NaCl concentration. The enzyme preserved 41% of its relative activity at 90°C. The partially purified lipase maintained its activity in the presence of surfactants such as Triton X-100 and SDS. Therefore, the lipase which is an extremozyme may have potential applications especially in detergent industry.

scholarly journals Extra- and intra-cellular lipases from a thermophilic Rhizopus oryzae and factors affecting their production

1993 ◽  
Vol 39 (10) ◽  
pp. 978-981 ◽  
Author(s):  
A. B. Salleh ◽  
R. Musani ◽  
M. Basri ◽  
K. Ampon ◽  
W. M. Z. Yunus ◽  
...  
Keyword(s):  
Enzyme Production ◽  
Rhizopus Oryzae ◽  
Corn Steep Liquor ◽  
Carbon Sources ◽  
Extracellular Enzyme ◽  
Lipase Production ◽  
Intracellular Enzyme ◽  
Factors Affecting ◽  
Positive Effects ◽  
Steep Liquor

A thermophilic Rhizopus oryzae was isolated, and parameters affecting its production of extra- and intra-cellular lipases were investigated. All carbon sources tested with the exception of sucrose generally inhibited the production of extracellular lipase, but enhanced the production of intracellular lipase. Peptone was the best substrate for extracellular enzyme production, but for intracellular lipase production other substrates such as tryptone, tryptic soy digest, polypeptone, and corn steep liquor gave comparable results. Among lipid substrates, glycerol was the only stimulator of extracellular enzyme production, whereas olive oil, triolein, and oleic acid had very positive effects on intracellular enzyme production. Shaking enhanced the production of both types of enzymes; the temperature optima were 45 and 37 °C for extra- and intra-cellular lipases, respectively. A pH of 5.0 was optimal for production of both enzymes.Key words: lipases, Rhizopus oryzae, production.

scholarly journals Assortment of carbon sources in medium for Yarrowia lipolytica lipase production: A statistical approach

2014 ◽  
Vol 65 (3) ◽  
pp. 1495-1503 ◽  
Author(s):  
Agata Urszula Fabiszewska ◽  
Danuta Kotyrba ◽  
Dorota Nowak
Keyword(s):  
Yarrowia Lipolytica ◽  
Statistical Approach ◽  
Carbon Sources ◽  
Lipase Production

Effect of different carbon sources on lipase production by Candida rugosa

2000 ◽  
Vol 26 (9-10) ◽  
pp. 657-663 ◽  
Author(s):  
E Dalmau ◽  
J.L Montesinos ◽  
M Lotti ◽  
C Casas
Keyword(s):  
Candida Rugosa ◽  
Carbon Sources ◽  
Lipase Production

Effects of carbon sources on extracellular lipase production and lip A transcription in Acinetobacter calcoaceticus

2000 ◽  
Vol 24 (1) ◽  
pp. 25-30 ◽  
Author(s):  
G F Mahler ◽  
R G Kok ◽  
A Cordenons ◽  
K J Hellingwerf ◽  
B C Nudel
Keyword(s):  
Carbon Sources ◽  
Acinetobacter Calcoaceticus ◽  
Lipase Production ◽  
Extracellular Lipase

Effect of Environmental and Nutritional Parameters on the Extracellular Lipase Production by Aspergillus niger

2016 ◽  
Vol 60 ◽  
pp. 18-29 ◽  
Author(s):  
Ahmed I. El-Batal ◽  
Ayman A. Farrag ◽  
Mohamed A. Elsayed ◽  
Ahmed M. El-Khawaga
Keyword(s):  
Aspergillus Niger ◽  
Incubation Temperature ◽  
Carbon Sources ◽  
Nitrogen Sources ◽  
Growth Conditions ◽  
Lipase Production ◽  
Extracellular Lipase ◽  
Chemical Parameters ◽  
Physical And Chemical Parameters ◽  
Physical And Chemical

Abstract- The present investigation was carried out to evaluate the effect of different growth conditions on lipase production byAspegillus niger. The extracellular lipase producing fungus was isolated from spent bleaching earths. Optimization of physical and chemical parameters was done for maximum lipase production using this isolate. Growth of the organism and lipase production were measured usig varying pH (4 – 9), incubation temperature (20 – 30 °C), incubation time (8 – 80 hrs.), carbon sources, nitrogen sources, and shaking speed. Enhanced lipase production was observed at 24 °C, pH 7 and after 72hrs of incubation. Olive oil 5 % was observed as the most effective carbon source and Yeast extract 1.0 % as the most effective nitrogen source for lipase production. The optimum shaking value to get maximum lipase activity byAspergillusnigerwas 200 rpm.

scholarly journals LIP4 gene expression from Candida viswanathii strain

2020 ◽  
Author(s):  
Ronan C. Teixeira ◽  
Matheus M. Daude ◽  
Nayra M. L. de Oliveira ◽  
Alex Fernando Almeida ◽  
Horllys G. Barreto
Keyword(s):  
Gene Expression ◽  
Olive Oil ◽  
Culture Media ◽  
Carbon Sources ◽  
In Silico Analysis ◽  
Lipase Production ◽  
Submerged Cultivation ◽  
Degenerate Primers ◽  
Candida Viswanathii ◽  
High Level

Abstract Background : Lipases are hydrolases that catalyze the cleavage of triglyceride esters bonds, releasing glycerol and free fatty acids. Candida genus can produce distinct isoforms of lipase, among of them, Candida viswanathii strain is a potential lipase producer using hydrophobic carbons sources in which produced high level of enzyme under submerged cultivation using olive oil as carbon sources. This enzyme has commercially attractive due to characteristics desired in the industries processes. The genes responsible for encoding the lipases comprise a family called LIP gene. C. viswanathii not have its genome sequenced and are not available in annotated form through the GenBank nucleotide sequence database for lipase production. The aim of this work was understanding at molecular level the effect of carbon sources to lipase production, to identify and to analyses the gene expression of CvLIP4 from C. viswanathii on different culture media. Results : In silico analysis was carried out with LIP4 gene from Candida species. Degenerate primers were designed and evaluated for expression in different conditions. CvLIP4 expression was evaluated using carbon sources glucose, tributyrin, triolein and olive oil. Triolein and olive oil were strong inducer for CvLIP4 gene expression, while tributyrin was a weak inducer and glucose was strong repressor. Conclusions : These results will contribute to further studies about regulation of the lipase genes expression from C. viswanathii and heterologous expression of this enzyme to improve the catalytic conditions in industries processes.

Sign in / Sign up

Export Citation Format

Share Document