Assortment of carbon sources in medium for Yarrowia lipolytica lipase production: A statistical approach

ABSTRACT The genes encoding gluconeogenic enzymes in the nonconventional yeast Yarrowia lipolytica were found to be differentially regulated. The expression of Y. lipolytica FBP1 (YlFBP1) encoding the key enzyme fructose-1,6-bisphosphatase was not repressed by glucose in contrast with the situation in other yeasts; however, this sugar markedly repressed the expression of YlPCK1, encoding phosphoenolpyruvate carboxykinase, and YlICL1, encoding isocitrate lyase. We constructed Y. lipolytica strains with two different disrupted versions of YlFBP1 and found that they grew much slower than the wild type in gluconeogenic carbon sources but that growth was not abolished as happens in most microorganisms. We attribute this growth to the existence of an alternative phosphatase with a high Km (2.3 mM) for fructose-1,6-bisphosphate. The gene YlFBP1 restored fructose-1,6-bisphosphatase activity and growth in gluconeogenic carbon sources to a Saccharomyces cerevisiae fbp1 mutant, but the introduction of the FBP1 gene from S. cerevisiae in the Ylfbp1 mutant did not produce fructose-1,6-bisphosphatase activity or growth complementation. Subcellular fractionation revealed the presence of fructose-1,6-bisphosphatase both in the cytoplasm and in the nucleus.

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Biofilm formation by a biotechnologically important tropical marine yeast isolate, Yarrowia lipolytica NCIM 3589

Water Science & Technology ◽

10.2166/wst.2008.479 ◽

2008 ◽

Vol 58 (6) ◽

pp. 1221-1229 ◽

Cited By ~ 11

Author(s):

D. H. Dusane ◽

Y. V. Nancharaiah ◽

V. P. Venugopalan ◽

A. R. Kumar ◽

S. S. Zinjarde

Keyword(s):

Yarrowia Lipolytica ◽

Biofilm Formation ◽

Edible Oils ◽

Carbon Sources ◽

Three Dimensional ◽

Ecological Niches ◽

Lag Phase ◽

Biofilm Growth ◽

Water Media ◽

Biofilm Development

Biofilm formation by Yarrowia lipolytica, a biotechnologically important fungus in microtitre plates, on glass slide surfaces and in flow cell was investigated. In microtitre plates, there was a short lag phase of adhesion followed by a period of rapid biofilm growth. The fungus formed extensive biofilms on glass slides, whereas in flow-cells a multicellular, three-dimensional microcolony structure was observed. The isolate formed biofilms in seawater and in fresh water media at neutral pH when grown in microtitre plates. The carbon sources differentially affected formation of biofilms in microtitre plates. Lactic acid, erythritol, glycerol, glucose and edible oils supported the formation of biofilms, while alkanes resulted in sub-optimal biofilm development. A variation in the morphology of the fungus was observed with different carbon sources. The results point to the possible existence of highly structured biofilms in varied ecological niches from where the yeast is isolated.

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The lipase activity of Talaromyces emersonii

Canadian Journal of Botany ◽

10.1139/b78-222 ◽

1978 ◽

Vol 56 (16) ◽

pp. 1840-1843 ◽

Cited By ~ 2

Author(s):

B. A. Oso

Keyword(s):

Liquid Medium ◽

Yeast Extract ◽

Lipase Activity ◽

Natural Habitat ◽

Carbon Sources ◽

Lipase Production ◽

Extracellular Lipase ◽

Talaromyces Emersonii ◽

Stationary Liquid ◽

Presence And Absence

Studies were carried out on the ability of Talaromyces emersonii Stolk to produce extracellular lipase in stationary liquid medium under various conditions. The best temperatures for lipase synthesis and activity were 40–45 °C, and at all the temperatures (37–55 °C) tested for lipase production, maximum enzyme was produced 8 days after incubation. Lipase synthesis was induced when various carbohydrates were used as carbon sources both in the presence and absence of yeast extract. The significance of lipase production in relation to the natural habitat is discussed.

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HOG-Independent Osmoprotection by Erythritol in Yeast Yarrowia lipolytica

Genes ◽

10.3390/genes11121424 ◽

2020 ◽

Vol 11 (12) ◽

pp. 1424

Author(s):

Dorota A. Rzechonek ◽

Mateusz Szczepańczyk ◽

Guokun Wang ◽

Irina Borodina ◽

Aleksandra M. Mirończuk

Keyword(s):

Transcription Factor ◽

Yarrowia Lipolytica ◽

Carbon Sources ◽

Hyperosmotic Stress ◽

Sensitive Strain ◽

The Other ◽

High Concentration ◽

Other Hand ◽

Yeast Yarrowia Lipolytica ◽

Better Than

Erythritol is a polyol produced by Yarrowia lipolytica under hyperosmotic stress. In this study, the osmo-sensitive strain Y. lipolytica yl-hog1Δ was subjected to stress, triggered by a high concentration of carbon sources. The strain thrived on 0.75 M erythritol medium, while the same concentrations of glucose and glycerol proved to be lethal. The addition of 0.1 M erythritol to the medium containing 0.75 M glucose or glycerol allowed the growth of yl-hog1Δ. Supplementation with other potential osmolytes such as mannitol or L-proline did not have a similar effect. To examine whether the osmoprotective effect might be related to erythritol accumulation, we deleted two genes involved in erythritol utilization, the transcription factor Euf1 and the enzyme erythritol dehydrogenase Eyd1. The strain eyd1Δ yl hog1Δ, which lacked the erythritol utilization enzyme, reacted to the erythritol supplementation significantly better than yl-hog1Δ. On the other hand, the strain euf1Δ yl-hog1Δ became insensitive to supplementation, and the addition of erythritol could no longer improve the growth of this strain in hyperosmotic conditions. This indicates that Euf1 regulates additional, still unknown genes involved in erythritol metabolism.

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