Effect of Environmental and Nutritional Parameters on the Extracellular Lipase Production by Aspergillus niger

2016 ◽  
Vol 60 ◽  
pp. 18-29 ◽  
Author(s):  
Ahmed I. El-Batal ◽  
Ayman A. Farrag ◽  
Mohamed A. Elsayed ◽  
Ahmed M. El-Khawaga

Abstract- The present investigation was carried out to evaluate the effect of different growth conditions on lipase production byAspegillus niger. The extracellular lipase producing fungus was isolated from spent bleaching earths. Optimization of physical and chemical parameters was done for maximum lipase production using this isolate. Growth of the organism and lipase production were measured usig varying pH (4 – 9), incubation temperature (20 – 30 °C), incubation time (8 – 80 hrs.), carbon sources, nitrogen sources, and shaking speed. Enhanced lipase production was observed at 24 °C, pH 7 and after 72hrs of incubation. Olive oil 5 % was observed as the most effective carbon source and Yeast extract 1.0 % as the most effective nitrogen source for lipase production. The optimum shaking value to get maximum lipase activity byAspergillusnigerwas 200 rpm.

2018 ◽  
Vol 6 (10) ◽  
pp. 123-130
Author(s):  
Yaroslava Zhukova ◽  
◽  
Pylyp Petrov ◽  
Olena Boloba ◽  
Tetiana Ohrimenko ◽  
...  

2013 ◽  
Vol 48 (1) ◽  
pp. 25-32 ◽  
Author(s):  
S Islam ◽  
B Feroza ◽  
AKMR Alam ◽  
S Begum

Pectinase activity among twelve different fungal strains, Aspergillus niger IM09 was identified as a potential one to produce maximal level 831 U/g at pH 4.0. Media composition, incubation temperature, incubation time, substrate concentration, aeration, inoculum size, assay temperature and nitrogen sources were found to effect pectinase activity. Moisture content did not affect the activity significantly. Media composition was varied to optimize the enzyme production in solid state fermentation. It was observed that the highest pectinase activity of 831.0 U/g was found to produce in presence of yeast extract as a nitrogen source in combination with ammonium sulfate in assay media. Aeration showed positive significant effects on pectinase production 755 U/g at 1000 ml flasks. The highest pectinase production was found at 2 g pectin (521 U/g) used as a substrate. Pectinolytic activity was found to have undergone catabolite repression with higher pectin concentration (205 U/g at 5 g pectin). The incubation period to achieve maximum pectinase activity by the isolated strain Aspergillus niger IM09 was 3 days, which is suitable from the commercial point of view. DOI: http://dx.doi.org/10.3329/bjsir.v48i1.15410 Bangladesh J. Sci. Ind. Res. 48(1), 25-32, 2013


2019 ◽  
Vol 8 (1) ◽  
pp. 20-30
Author(s):  
Rangga Bayu Kusuma Haris ◽  
Indah Anggraini Yusanti

Haris et al, 2019. The Analysis of Water Suitability for Floating Net Sprouts in Sirah Pulau Padang, Ogan Komering Ilir District, South Sumatra Province. JLSO 8(1):20-30.The purpose of this study is to analyze the level of water suitability of the floating net sprouts in sirah pulau padang, ogan komering river, Serdang Menang Village, OKI South Sumatra Regency based on water physical and chemical parameters. This study was conducted on July - August 2018 at the Komering River, Ulak Jermun village, Terusan Menang Village, and Mangun Jaya Village, OKI District, South Sumatra. The data of sampling was divided into 3 stations, they were; station 1 was Ulak Jermun Village (3 ° 18'45.48 "LS and 104 ° 53'5.32" BT), station 2 was Terusan Menang Village (3 ° 17'14.13 "LS and 104 ° 52'46.78 "BT), and station 3 was Mangun Jaya village (3 ° 18'14.92" LS and 104 ° 52'59.48 "BT). The data of Samples were tested at the chemical laboratory in Palembang Public Water Fisheries and Extension Research Institute directly. The results of the study was for Station 1, Station 2, and Station 3 with a value was  48% with a good feasibility information. The results of measurements of water quality parameters, the pH was about 6.6 - 6.7, temperature was about from 24., 53 - 26.93oC, DO 3.93 - 4.36 mg / l, ammonia was about 0.78 - 0.16 mg / l, water brightness was about from 34.67 - 43.33 cm, CO2 4.53 - 5.03 mg / l, depth 2.36 - 2.47 m, current velocity was about 0.38 - 0.44 m / s, nitrate was about 0.37 - 0.439 mg / l, phosphate was about 0.009 - 0.225 mg / l and the bottom substrate of water was mud.


2019 ◽  
Vol 20 (9) ◽  
Author(s):  
MOHAMMAD REZA MOHSENI ◽  
SHAHROKH PASHAEI RAD

The present study was conducted in Salt marshes and Salt pans in central parts of Iran. 121 specimens of ants were collected by trapping and hand during spring, summer and autumn 2017 to 2019. Nine species belong to two subfamilies of Myrmicinae and Formicinae were identified. The vegetation, physical and chemical parameters of soil such as salinity, Ph, and other factors were investigated. Darband Shoor Mountain station with five species had the highest diversity and vegetation density and the lowest salinity of soil (72.14 ds/m) whereas Salt Lake station had only one species, with no vegetation and the highest salinity of soil (153.15 ds/m). Cataglyphis lividus (André, 1881) was the dominant species in all the stations except in Darband Shoor Mountain station. All the species were reported for the first time from salt marshes and salt pans in the central parts of Iran and were approved by Prof. Brian Taylor, in Royal Entomological Society of London, England.


Author(s):  
Dewi Nuryanti Fazrin ◽  
Hasim Hasim ◽  
Juliana Juliana

The purpose of this study was to determine the relationship between the length and weight of Manggabai fish (Glossogobius giuris) and to know the relationship between the presence of Manggabai fish (Glossogobius giuris) and water quality in Limboto Lake. This research was conducted in February 2019, located in the waters of Lake Limboto, Gorontalo Province. The method used in determining the location of sampling is Purposive Sampling. Fish sampling is determined by considering fishing locations, litoral areas, the presence of residential areas, agricultural and plantation areas, the existence of floating net cages and in the middle of the lake. The study was conducted at 20 observation stations using fish traps in the form of bamboo and used motorcycle tires that have been modified and become traditional fishing gear. The results showed that the physical and chemical parameters in Lake Limboto and showed temperature values ​​ranged from 29.77 to 31.630C, dissolved oxygen levels ranged from 2.1 to 5.48 ppm, pH ranged from 7.09 to 8.94, TDS ranging from 0.251 to 0.369, Nitrite Levels 0.04-0.08 ppm, Nitrate Levels 1.2-4.7 ppm, BOD ranges from 2.5-57 ppm and phosphate levels 0.1-1.14 ppm. The relationship between length and body weight of fish are allometric, where W = 0.8769 L10,023 and constant b (10,023)> 3.


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