Differential expression of arid5b isoforms in Xenopus laevis pronephros

An extract from whole oocytes of Xenopus laevis was shown to transcribe somatic-type 5S RNA genes approximately 100-fold more efficiently than oocyte-type 5S RNA genes. This preference was at least 10-fold greater than the preference seen upon microinjection of 5S RNA genes into oocyte nuclei or upon in vitro transcription in an oocyte nuclear extract. The approximately 100-fold transcriptional bias in favor of the somatic-type 5S RNA genes observed in vitro in the whole oocyte extract was similar to the transcriptional bias observed in developing Xenopus embryos. We also showed that in the whole oocyte extract, a promoter-binding protein required for 5S RNA gene transcription, TFIIIA, was bound both to the actively transcribed somatic-type 5S RNA gene and to the largely inactive oocyte-type 5S RNA genes. These findings suggest that the mechanism for the differential expression of 5S RNA genes during Xenopus development does not involve differential binding of TFIIIA to 5S RNA genes.

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Effect of sequence differences between somatic and oocyte 5S RNA genes on transcriptional efficiency in an oocyte S150 extract

Molecular and Cellular Biology ◽

10.1128/mcb.8.11.5056-5058.1988 ◽

1988 ◽

Vol 8 (11) ◽

pp. 5056-5058

Author(s):

W F Reynolds

Keyword(s):

Xenopus Laevis ◽

Differential Expression ◽

Internal Control ◽

Transcriptional Activity ◽

Stable Complex ◽

5S Rna ◽

Internal Control Region ◽

5S Rna Genes ◽

Rna Genes ◽

5S Gene

The differential expression of the Xenopus laevis somatic and oocyte 5S RNA genes is partially, but not solely, due to several base differences near the 5' boundary of the internal control region. A hybrid oocyte 5S gene with somatic-type base changes at +47, +53, +55, and +56 had intermediate transcriptional activity in oocyte S150 extracts. These base substitutions also resulted in increased affinity for a factor(s), other than TFIIIA, which forms a stable complex with the 5S gene.

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Cloning and Differential Expression of New Calcium, Calmodulin- Dependent Protein Kinase II Isoforms in Xenopus laevis Oocytes and Several Adult Tissues

The Journal of Biochemistry ◽

10.1093/oxfordjournals.jbchem.a002890 ◽

2001 ◽

Vol 129 (4) ◽

pp. 551-560 ◽

Cited By ~ 6

Author(s):

I. Stevens ◽

E. Rondelez ◽

W. Merlevede ◽

J. Goris

Keyword(s):

Protein Kinase ◽

Xenopus Laevis ◽

Differential Expression ◽

Xenopus Laevis Oocytes ◽

Dependent Protein Kinase ◽

Calcium Calmodulin Dependent ◽

Adult Tissues ◽

Protein Kinase Ii ◽

Dependent Protein

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Differential expression of the Xenopus laevis tadpole and adult beta-globin genes when injected into fertilized Xenopus laevis eggs.

Molecular and Cellular Biology ◽

10.1128/mcb.4.3.567 ◽

1984 ◽

Vol 4 (3) ◽

pp. 567-570 ◽

Cited By ~ 15

Author(s):

M M Bendig ◽

J G Williams

Keyword(s):

Xenopus Laevis ◽

Differential Expression ◽

Maximum Level ◽

Globin Genes ◽

Beta Globin ◽

Stages Of Development ◽

Chromosomal Genes ◽

Xenopus Laevis Tadpole

Xenopus laevis tadpole and adult beta-globin genes were injected into fertilized X. laevis eggs. Both injected genes replicated and were retained in the developing embryos with equal efficiency. Transcripts of the injected adult gene were detectable at gastrulation and reached a maximum level shortly thereafter. In contrast, transcripts of the injected tadpole gene were not detected until much later stages of development. The level of expression of both the injected genes was low compared with the level of expression of the chromosomal genes during erythropoiesis.

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Transcriptionally inactive oocyte-type 5S RNA genes of Xenopus laevis are complexed with TFIIIA in vitro

Molecular and Cellular Biology ◽

10.1128/mcb.7.10.3503-3510.1987 ◽

1987 ◽

Vol 7 (10) ◽

pp. 3503-3510

Author(s):

L J Peck ◽

L Millstein ◽

P Eversole-Cire ◽

J M Gottesfeld ◽

A Varshavsky

Keyword(s):

Xenopus Laevis ◽

Differential Expression ◽

Gene Transcription ◽

Nuclear Extract ◽

In Vitro Transcription ◽

5S Rna ◽

Differential Binding ◽

5S Rna Genes ◽

Rna Genes

An extract from whole oocytes of Xenopus laevis was shown to transcribe somatic-type 5S RNA genes approximately 100-fold more efficiently than oocyte-type 5S RNA genes. This preference was at least 10-fold greater than the preference seen upon microinjection of 5S RNA genes into oocyte nuclei or upon in vitro transcription in an oocyte nuclear extract. The approximately 100-fold transcriptional bias in favor of the somatic-type 5S RNA genes observed in vitro in the whole oocyte extract was similar to the transcriptional bias observed in developing Xenopus embryos. We also showed that in the whole oocyte extract, a promoter-binding protein required for 5S RNA gene transcription, TFIIIA, was bound both to the actively transcribed somatic-type 5S RNA gene and to the largely inactive oocyte-type 5S RNA genes. These findings suggest that the mechanism for the differential expression of 5S RNA genes during Xenopus development does not involve differential binding of TFIIIA to 5S RNA genes.

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Differential expression of presenilin-α and -β (PSα and PSβ) in Xenopus laevis: embryonic phosphorylation of PSα

Gene ◽

10.1016/s0378-1119(03)00726-1 ◽

2003 ◽

Vol 314 ◽

pp. 165-172 ◽

Cited By ~ 1

Author(s):

Yoshihisa Watanabe ◽

Atsushi Tsujimura ◽

Takeshi Tabira ◽

Tamotsu Hashimoto-Gotoh

Keyword(s):

Xenopus Laevis ◽

Differential Expression

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Effect of sequence differences between somatic and oocyte 5S RNA genes on transcriptional efficiency in an oocyte S150 extract.

Molecular and Cellular Biology ◽

10.1128/mcb.8.11.5056 ◽

1988 ◽

Vol 8 (11) ◽

pp. 5056-5058 ◽

Cited By ~ 5

Author(s):

W F Reynolds

Keyword(s):

Xenopus Laevis ◽

Differential Expression ◽

Internal Control ◽

Transcriptional Activity ◽

Stable Complex ◽

5S Rna ◽

Internal Control Region ◽

5S Rna Genes ◽

Rna Genes ◽

5S Gene

The differential expression of the Xenopus laevis somatic and oocyte 5S RNA genes is partially, but not solely, due to several base differences near the 5' boundary of the internal control region. A hybrid oocyte 5S gene with somatic-type base changes at +47, +53, +55, and +56 had intermediate transcriptional activity in oocyte S150 extracts. These base substitutions also resulted in increased affinity for a factor(s), other than TFIIIA, which forms a stable complex with the 5S gene.

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