Differential expression of two TEF-1 (TEAD) genes during Xenopus laevis development and in response to inducing factors

2007 ◽  
Vol 51 (8) ◽  
pp. 745-752 ◽  
Author(s):  
Francois Naye ◽  
Karine Treguer ◽  
Fabienne Soulet ◽  
Corinne Faucheux ◽  
Sandrine Fedou ◽  
...  
Keyword(s):  
Xenopus Laevis ◽  
Differential Expression ◽  
Inducing Factors

Differential expression of the TFF-peptides xP1 and xP4 in the gastrointestinal tract of Xenopus laevis

1997 ◽  
Vol 291 (1) ◽  
pp. 13-18 ◽  
Author(s):  
Wolfgang Jagla ◽  
Antje Wiede ◽  
Sabine K�lle ◽  
W. Hoffmann
Keyword(s):  
Gastrointestinal Tract ◽  
Xenopus Laevis ◽  
Differential Expression ◽  
Tff Peptides

scholarly journals Transcriptionally inactive oocyte-type 5S RNA genes of Xenopus laevis are complexed with TFIIIA in vitro.

1987 ◽  
Vol 7 (10) ◽  
pp. 3503-3510 ◽  
Author(s):  
L J Peck ◽  
L Millstein ◽  
P Eversole-Cire ◽  
J M Gottesfeld ◽  
A Varshavsky
Keyword(s):  
Xenopus Laevis ◽  
Differential Expression ◽  
Gene Transcription ◽  
Nuclear Extract ◽  
In Vitro Transcription ◽  
5S Rna ◽  
Differential Binding ◽  
5S Rna Genes ◽  
Rna Genes

An extract from whole oocytes of Xenopus laevis was shown to transcribe somatic-type 5S RNA genes approximately 100-fold more efficiently than oocyte-type 5S RNA genes. This preference was at least 10-fold greater than the preference seen upon microinjection of 5S RNA genes into oocyte nuclei or upon in vitro transcription in an oocyte nuclear extract. The approximately 100-fold transcriptional bias in favor of the somatic-type 5S RNA genes observed in vitro in the whole oocyte extract was similar to the transcriptional bias observed in developing Xenopus embryos. We also showed that in the whole oocyte extract, a promoter-binding protein required for 5S RNA gene transcription, TFIIIA, was bound both to the actively transcribed somatic-type 5S RNA gene and to the largely inactive oocyte-type 5S RNA genes. These findings suggest that the mechanism for the differential expression of 5S RNA genes during Xenopus development does not involve differential binding of TFIIIA to 5S RNA genes.

scholarly journals Differential expression of arid5b isoforms in Xenopus laevis pronephros

2014 ◽  
Vol 58 (5) ◽  
pp. 363-368
Author(s):  
Ronan Le Bouffant ◽  
Anne-Claire Cunin ◽  
Isabelle Buisson ◽  
Jérôme Cartry ◽  
Jean-François Riou ◽  
...  
Keyword(s):  
Xenopus Laevis ◽  
Differential Expression

Inducing factors and the control of mesodermal pattern in Xenopus laevis

Development ◽  
1989 ◽  
Vol 107 (Supplement) ◽  
pp. 149-159 ◽  
Author(s):  
J. C. Smith ◽  
J. Cooke ◽  
J. B. A. Green ◽  
G. Howes ◽  
K. Symes
Keyword(s):  
Growth Factor ◽  
Xenopus Laevis ◽  
Transforming Growth Factor ◽  
Cell Types ◽  
The Other ◽  
Fibroblast Growth ◽  
Animal Pole ◽  
Spemann's Organizer ◽  
Inductive Interaction ◽  
Inducing Factors

The mesoderm of Xenopus laevis and other amphibia is formed through an inductive interaction during which cells of the vegetal hemisphere act on cells of the animal hemisphere. Two groups of factors mimic the effects of the vegetal hemisphere. One group consists of members of the fibroblast growth factor (FGF) family, while the other is related to transforming growth factor typeβ(TGF-β). In this paper we discuss the evidence that the FGF family represents ‘ventral’ mesoderm-inducing signals, and the TGF-β family ‘dorsal’ signals. The evidence includes a discussion of the cell types formed in response to each type of factor, the fact that only XTCMIF (a member of the TGF-β family) and not bFGF can induce animal pole ectoderm to become Spemann's organizer, and an analysis of the timing of the gastrulation movements induced by the factors.

Effect of sequence differences between somatic and oocyte 5S RNA genes on transcriptional efficiency in an oocyte S150 extract

1988 ◽  
Vol 8 (11) ◽  
pp. 5056-5058
Author(s):  
W F Reynolds
Keyword(s):  
Xenopus Laevis ◽  
Differential Expression ◽  
Internal Control ◽  
Transcriptional Activity ◽  
Stable Complex ◽  
5S Rna ◽  
Internal Control Region ◽  
5S Rna Genes ◽  
Rna Genes ◽  
5S Gene

The differential expression of the Xenopus laevis somatic and oocyte 5S RNA genes is partially, but not solely, due to several base differences near the 5' boundary of the internal control region. A hybrid oocyte 5S gene with somatic-type base changes at +47, +53, +55, and +56 had intermediate transcriptional activity in oocyte S150 extracts. These base substitutions also resulted in increased affinity for a factor(s), other than TFIIIA, which forms a stable complex with the 5S gene.

scholarly journals Neural inducing factors from Xenopus laevis eggs and embryos

1999 ◽  
Vol 41 (2) ◽  
pp. 201-205 ◽  
Author(s):  
Heinz Tiedemann ◽  
Hildegard Tiedemann
Keyword(s):  
Xenopus Laevis ◽  
Inducing Factors

scholarly journals Differential expression of the Xenopus laevis tadpole and adult beta-globin genes when injected into fertilized Xenopus laevis eggs.

1984 ◽  
Vol 4 (3) ◽  
pp. 567-570 ◽  
Author(s):  
M M Bendig ◽  
J G Williams
Keyword(s):  
Xenopus Laevis ◽  
Differential Expression ◽  
Maximum Level ◽  
Globin Genes ◽  
Beta Globin ◽  
Stages Of Development ◽  
Chromosomal Genes ◽  
Xenopus Laevis Tadpole

Xenopus laevis tadpole and adult beta-globin genes were injected into fertilized X. laevis eggs. Both injected genes replicated and were retained in the developing embryos with equal efficiency. Transcripts of the injected adult gene were detectable at gastrulation and reached a maximum level shortly thereafter. In contrast, transcripts of the injected tadpole gene were not detected until much later stages of development. The level of expression of both the injected genes was low compared with the level of expression of the chromosomal genes during erythropoiesis.

scholarly journals Transcriptionally inactive oocyte-type 5S RNA genes of Xenopus laevis are complexed with TFIIIA in vitro

1987 ◽  
Vol 7 (10) ◽  
pp. 3503-3510
Author(s):  
L J Peck ◽  
L Millstein ◽  
P Eversole-Cire ◽  
J M Gottesfeld ◽  
A Varshavsky
Keyword(s):  
Xenopus Laevis ◽  
Differential Expression ◽  
Gene Transcription ◽  
Nuclear Extract ◽  
In Vitro Transcription ◽  
5S Rna ◽  
Differential Binding ◽  
5S Rna Genes ◽  
Rna Genes

An extract from whole oocytes of Xenopus laevis was shown to transcribe somatic-type 5S RNA genes approximately 100-fold more efficiently than oocyte-type 5S RNA genes. This preference was at least 10-fold greater than the preference seen upon microinjection of 5S RNA genes into oocyte nuclei or upon in vitro transcription in an oocyte nuclear extract. The approximately 100-fold transcriptional bias in favor of the somatic-type 5S RNA genes observed in vitro in the whole oocyte extract was similar to the transcriptional bias observed in developing Xenopus embryos. We also showed that in the whole oocyte extract, a promoter-binding protein required for 5S RNA gene transcription, TFIIIA, was bound both to the actively transcribed somatic-type 5S RNA gene and to the largely inactive oocyte-type 5S RNA genes. These findings suggest that the mechanism for the differential expression of 5S RNA genes during Xenopus development does not involve differential binding of TFIIIA to 5S RNA genes.

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