scholarly journals Studies on immune response to Newcastle disease virus in broiler chickens fed with Lactobacillus reuteri PIA16 isolated from the gut of indigenous chicken of Assam, India

2019 ◽  
Vol 12 (8) ◽  
pp. 1251-1255 ◽  
Author(s):  
Gaichamdinliu Gonmei ◽  
Deben Sapcota ◽  
Girin Kumar Saikia ◽  
Pankaj Deka ◽  
Joga Dev Mahanta ◽  
...  
Keyword(s):  
Immune Response ◽  
Newcastle Disease Virus ◽  
Disease Virus ◽  
Newcastle Disease ◽  
Hemagglutination Inhibition ◽  
Broiler Chickens ◽  
Lactobacillus Reuteri ◽  
Production Performance ◽  
Indigenous Chicken ◽  
Mannan Oligosaccharide

Background and Aim: The chicken gut harbors microflora which impacts the health, production performance and immune response against pathogens. Assam local chickens reared under natural conditions are known to possess high immunocompetence which may be attributable to its gut microbiota make-up. This study aimed to investigate the individual effect of two strains of Lactobacillus reuteri PIA16 isolated separately from cecum and jejunum of Assam indigenous chicken on the immunity of broiler chickens against Newcastle disease virus (NDV) when fed singly and in combination with a prebiotic. Materials and Methods: A total of 240 birds (48 per group) were vaccinated with Lasota strain of NDV on the 5th and 21st day of age. Blood samples were collected before and after immunization against ND for the detection of humoral antibody response by hemagglutination inhibition test. The cell-mediated immune (CMI) response was estimated through response to phytohemagglutinin-P (PHA-P) and expressed as web index. Results: A significant influence on the immune response to NDV was observed in all the L. reuteri PIA16 as well as mannan oligosaccharide (MOS) supplemented groups revealing higher antibody titer than the control counterpart. The CMI response revealed a better cutaneous basophilic hypersensitivity response to PHA-P in the treated groups than the control. Conclusion: Enhancement in immunity was perceived in the broilers fed with L. reuteri PIA16 and in combination with MOS due to the stimulation of the host's humoral and CMI response by the probiotics and prebiotics used. Keywords: hemagglutination inhibition, Lactobacillus reuteri PIA16, mannan oligosaccharide, Newcastle disease, phytohemagglutinin-P, probiotics.

scholarly journals Effect of essential oils on the immune response to some viral vaccines in broiler chickens, with special reference to Newcastle disease virus

2020 ◽  
Vol 99 (6) ◽  
pp. 2944-2954
Author(s):  
Nahed A. El-Shall ◽  
Ramadan S. Shewita ◽  
Mohamed E. Abd El-Hack ◽  
Abdullah AlKahtane ◽  
Saud Alarifi ◽  
...  
Keyword(s):  
Immune Response ◽  
Newcastle Disease Virus ◽  
Essential Oils ◽  
Special Reference ◽  
Disease Virus ◽  
Newcastle Disease ◽  
Broiler Chickens ◽  
Viral Vaccines

scholarly journals Growth performance and immune response to Newcastle disease in four gene pools of indigenous chicken in Rwanda

2020 ◽  
Vol 1 (2) ◽  
pp. 42-50
Author(s):  
Richard Habimana ◽  
Kiplangat Ngeno ◽  
Anselme Shyaka ◽  
Martin Ntawubizi ◽  
Janvier Mahoro ◽  
...  
Keyword(s):  
Immune Response ◽  
Newcastle Disease Virus ◽  
Growth Performance ◽  
Disease Virus ◽  
Newcastle Disease ◽  
Gene Pool ◽  
Growth Patterns ◽  
Gene Pools ◽  
Indigenous Chicken ◽  
Newcastle Disease Vaccine

In Rwanda, the most prevalent livestock farming activity carried out to improve livelihoods in rural areas is poultry farming. The most common bird species raised for egg and meat production is indigenous chicken (IC). Despite its economic significance, the improvement programs in Rwanda have given IC little attention. There is, therefore, little documentation on their production and disease resistance performance. This study aimed at evaluating the growth performance and immunologic response of IC against Newcastle disease vaccine in four gene pools in Rwanda. One hundred eighty-nine (189) IC from four gene pools were kept on-station using a completely randomized design. Thereafter, the IC were vaccinated with a live commercial Newcastle disease virus. Data on body weight were collected weekly for 20 weeks whereas serum samples were collected after 35 days. Antibody responses were determined using indirect ELISA. Analysis of variance was performed using the SAS Generalised Linear Model procedure. Growth patterns were modelled using the logistic function. Results revealed a highly significant difference (P<0.001) for growth performance and antibody response to Newcastle disease vaccine among the four gene pools. Gene pool A was the heaviest (1.6 kg) in the period of 20 weeks and gene pool C expressed the highest immune response (8,161antibody titres) for Newcastle disease vaccine (P<0.001). The results indicated that the selection of gene pool A in breeding could yield IC with good growth performance trait whilst gene pool C could be selected for its higher immunity against Newcastle disease virus.

scholarly journals Optimization of Human Immunodeficiency Virus Gag Expression by Newcastle Disease Virus Vectors for the Induction of Potent Immune Responses

2008 ◽  
Vol 83 (2) ◽  
pp. 584-597 ◽  
Author(s):  
Elena Carnero ◽  
Wenjing Li ◽  
Antonio V. Borderia ◽  
Bruno Moltedo ◽  
Thomas Moran ◽  
...  
Keyword(s):  
Immune Response ◽  
Human Immunodeficiency Virus ◽  
Immune Responses ◽  
Newcastle Disease Virus ◽  
Disease Virus ◽  
Newcastle Disease ◽  
Insertion Site ◽  
Gag Protein ◽  
Immunodeficiency Virus

ABSTRACT One attractive strategy for the development of a human immunodeficiency virus (HIV) vaccine is the use of viral vectors with a proven safety profile and an absence of preexisting immunity in humans, such as Newcastle disease virus (NDV). Several NDV vaccine vectors have been generated, and their immunogenicities have been investigated with different animal models. However, a systematic study to evaluate the optimal insertion site of the foreign antigens into NDV that results in enhanced immune responses specific to the antigen has not yet been conducted. In this article, we describe the ability of NDV expressing HIV Gag to generate a Gag-specific immune response in mice. We also have determined the optimal insertion site into the NDV genome by generating recombinant NDV-HIVGag viruses in which HIV gag was located at different transcriptional positions throughout the NDV viral genome. All recombinant viruses were viable, grew to similar titers in embryonated chicken eggs, and expressed Gag in a stable manner. Our in vivo experiments revealed that higher HIV Gag protein expression positively correlates with an enhanced CD8+ T-cell-mediated immune response and protective immunity against challenge with vaccinia virus expressing HIV Gag. We also inserted a codon-optimized version of HIV gag in the described best location, between the P and M genes. Virus expressing the codon-optimized version of HIV gag induced a higher expression of the protein and an enhanced immune response against HIV Gag in mice. These results indicate that strategies directed toward increasing antigen expression by NDV result in enhanced immunogenicity and vaccine efficacy.

Sign in / Sign up

Export Citation Format

Share Document