scholarly journals Effects of hydrolyzable tannin extract obtained from sweet chestnut wood (Castanea sativa Mill.) against bacteria causing subclinical mastitis in Thai Friesian dairy cows

2021 ◽  
pp. 2427-2433
Author(s):  
Tipwadee Prapaiwong ◽  
Wuttikorn Srakaew ◽  
Chalong Wachirapakorn ◽  
Chaiwat Jarassaeng
Keyword(s):  
Antibacterial Activity ◽  
Castanea Sativa ◽  
Inhibition Zone ◽  
Subclinical Mastitis ◽  
Tannin Extract ◽  
Hydrolyzable Tannin ◽  
Sweet Chestnut ◽  
Hydrolyzable Tannins ◽  
Castanea Sativa Mill

Background and Aim: Hydrolyzable tannins are an important group of secondary plant metabolites, which are known for antimicrobial activity. This study aimed to assess the efficiency with which a hydrolyzable tannin extract from sweet chestnut wood (Castanea sativa Mill.) could inhibit mastitis-causing bacteria in vitro. Materials and Methods: The negative control used was sterile water, and the positive controls were penicillin and gentamicin. The treatments included five concentrations of hydrolyzable tannins (63, 190, 313, 630, and 940 mg/mL). In cows with subclinical mastitis, the bacteria causing the disease were isolated and identified. Then, the antibacterial activity of the hydrolyzable tannin extract was assessed by the disk diffusion method, by determining the minimum inhibitory concentration (MIC) and by determining the minimum bactericidal concentration (MBC). Results: Penicillin inhibited (p<0.01) the growth of Staphylococcus aureus, Streptococcus uberis, and Pseudomonas aeruginosa but could not inhibit (p>0.05) the growth of Streptococcus agalactiae, Escherichia coli, and Klebsiella pneumoniae. However, gentamicin and hydrolyzable tannins could inhibit (p<0.01) all isolated bacteria. Increasing the concentration of hydrolyzable tannin extract resulted in a quadratic increase in the inhibition zone diameter of S. aureus and S. agalactiae and a linear increase in the inhibition zone diameter of E. coli, K. pneumoniae, and P. aeruginosa. In addition, 630 and 940 mg/mL of hydrolyzable tannin extract showed the highest antibacterial activity against S. agalactiae and E. coli (p<0.01), while 940 mg/mL concentration had the highest antibacterial activity against K. pneumoniae (p<0.01). The MIC and MBC of the extract were 27.3-190 mg/mL and 58.8-235 mg/mL, respectively, with the MBC: MIC ratio being 2:1. Conclusion: The antimicrobial activity of the hydrolyzable tannin extract against subclinical mastitis bacteria was comparable to the antibiotics (positive controls) at concentrations over 630 mg/mL. Although these in vitro findings are promising, further research is needed to determine whether hydrolyzable tannins could be used to control or prevent subclinical mastitis in dairy cows.

scholarly journals Hydrolyzable Tannins from Sweet Chestnut Fractions Obtained by a Sustainable and Eco-friendly Industrial Process

2016 ◽  
Vol 11 (3) ◽  
pp. 1934578X1601100 ◽  
Author(s):  
Margherita Campo ◽  
Patrizia Pinelli ◽  
Annalisa Romani
Keyword(s):  
Gallic Acid ◽  
Membrane Separation ◽  
Industrial Process ◽  
Castanea Sativa ◽  
Chemical Compositions ◽  
Sweet Chestnut ◽  
Technology System ◽  
Test Range ◽  
Hydrolyzable Tannins ◽  
Castanea Sativa Mill

Sweet Chestnut ( Castanea sativa Mill.) wood extracts, rich in Hydrolyzable Tannins (HTs), are traditionally used in the tanning and textile industries, but recent studies suggest additional uses. The aim of this work is the HPLC-DAD-ESI-MS characterization of Sweet Chestnut aqueous extracts and fractions obtained through a membrane separation technology system without using other solvents, and the evaluation of their antioxidant and antiradical activities. Total tannins range between 2.7 and 138.4 mM; gallic acid ranges between 6% and 100%; castalagin and vescalagin range between 0% and 40%. Gallic Acid Equivalents, measured with the Folin-Ciocalteu test, range between 0.067 and 56.99g/100g extract weight; ORAC test results for the marketed fractions are 450.4 and 3050 μmol/g Trolox Equivalents/extract weight. EC50 values, measured with the DPPH test, range between 0.444 and 2.399 μM. These results suggest a new ecofriendly and economically sustainable method for obtaining chestnut fractions with differentiated, stable and reproducible chemical compositions. Such fractions can be marketed for innovative uses in several sectors.

scholarly journals Effect of ecological conditions on expression of biopomological characteristics of chestnut (Castanea sativa Mill.) in natural populations of Montenegro

Genetika ◽  
2013 ◽  
Vol 45 (1) ◽  
pp. 251-260 ◽  
Author(s):  
Aleksandar Odalovic ◽  
Ranko Prenkic ◽  
Darko Dubak ◽  
Miodrag Jovancevic ◽  
Miroslav Cizmovic ◽  
...  
Keyword(s):  
Natural Populations ◽  
Castanea Sativa ◽  
Fruit Weight ◽  
Average Yield ◽  
Sweet Chestnut ◽  
Full Flowering ◽  
Castanea Sativa Mill ◽  
Length Width ◽  
Very High

This paper presents the five-year long research results (2007-2011) of phenological observations (beginning, full flowering and the end of flowering), morphometric analysis (fruit weight, length, width and thickness of fruit, as well as length and width of the hilum), the ripening time and the average yield of selected genotypes of sweet chestnut (Castanea sativa Mill.). The results show that in terms of time of flowering chestnut trees examined in the group are very early flowering. The ripening of studied chestnut trees shows that the earliest harvest tree was Ostros II, VII and Kostajnica V (09.11.), and Kostajnica III (14.11.) were the latest. The average fruit weight of the selected sweet chestnut trees was (6.9 g). The highest average fruit weight (10.6 g), had the examined Ostros I tree, and the lowest (4.8 g), Kostajnica VI. The yield of the tested chestnut trees was on average (76.3 kg / tree). The selected trees Kostajnica II (66.0 kg / tree), Kostajnica I (69.0 kg / tree) had the smallest yield and Ostros VI (94.0 kg / tree) and Ostros V (87.0 kg / tree) had the highest. Obviously it can be concluded that population genetic variability is very high and gene expression is highly affected in tested samples on both locations. For further research it should be given attention to investigate genotypes in controlled conditions, the best in vitro in tissue culture.

scholarly journals The Dynamics of Flower Development in Castanea Sativa Mill.

Plants ◽  
2021 ◽  
Vol 10 (8) ◽  
pp. 1538
Author(s):  
Ana Teresa Alhinho ◽  
Miguel Jesus Nunes Ramos ◽  
Sofia Alves ◽  
Margarida Rocheta ◽  
Leonor Morais-Cecílio ◽  
...  
Keyword(s):  
Flower Development ◽  
Molecular Mechanisms ◽  
Castanea Sativa ◽  
Male And Female ◽  
Sweet Chestnut ◽  
Chestnut Tree ◽  
Abcde Model ◽  
Organ Identity ◽  
Castanea Sativa Mill ◽  
Female Flowers

The sweet chestnut tree (Castanea sativa Mill.) is one of the most significant Mediterranean tree species, being an important natural resource for the wood and fruit industries. It is a monoecious species, presenting unisexual male catkins and bisexual catkins, with the latter having distinct male and female flowers. Despite the importance of the sweet chestnut tree, little is known regarding the molecular mechanisms involved in the determination of sexual organ identity. Thus, the study of how the different flowers of C. sativa develop is fundamental to understand the reproductive success of this species and the impact of flower phenology on its productivity. In this study, a C. sativa de novo transcriptome was assembled and the homologous genes to those of the ABCDE model for floral organ identity were identified. Expression analysis showed that the C. sativa B- and C-class genes are differentially expressed in the male flowers and female flowers. Yeast two-hybrid analysis also suggested that changes in the canonical ABCDE protein–protein interactions may underlie the mechanisms necessary to the development of separate male and female flowers, as reported for the monoecious Fagaceae Quercus suber. The results here depicted constitute a step towards the understanding of the molecular mechanisms involved in unisexual flower development in C. sativa, also suggesting that the ABCDE model for flower organ identity may be molecularly conserved in the predominantly monoecious Fagaceae family.

scholarly journals Biocultural diversity of common walnut ( Juglans regia L.) and sweet chestnut ( Castanea sativa Mill.) across Eurasia

2020 ◽  
Vol 10 (20) ◽  
pp. 11192-11216
Author(s):  
Paola Pollegioni ◽  
Stefano Del Lungo ◽  
Ruth Müller ◽  
Keith E. Woeste ◽  
Francesca Chiocchini ◽  
...  
Keyword(s):  
Juglans Regia ◽  
Castanea Sativa ◽  
Biocultural Diversity ◽  
Sweet Chestnut ◽  
Castanea Sativa Mill ◽  
Common Walnut

scholarly journals In vitro SCREENING ANTIBACTERIAL ACTIVITY OF Bidens pilosa LINNÉ AND Annona crassiflora MART. AGAINST OXACILLIN RESISTANT Staphylococcus aureus (ORSA) FROM THE AERIAL ENVIRONMENT AT THE DENTAL CLINIC

2014 ◽  
Vol 56 (4) ◽  
pp. 333-340 ◽  
Author(s):  
Jeferson Junior da Silva ◽  
Cláudio Daniel Cerdeira ◽  
Juliana Moscardini Chavasco ◽  
Ana Beatriz Pugina Cintra ◽  
Carla Brigagão Pacheco da Silva ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Toxicity Testing ◽  
Inhibition Zone ◽  
Bidens Pilosa ◽  
Total Absence ◽  
Mtt Method ◽  
In Vitro Antibacterial Activity ◽  
Annona Crassiflora

Currently multiresistant Staphylococcus aureus is one common cause of infections with high rates of morbidity and mortality worldwide, which directs scientific endeavors in search for novel antimicrobials. In this study, nine extracts from Bidens pilosa (root, stem, flower and leaves) and Annona crassiflora (rind fruit, stem, leaves, seed and pulp) were obtained with ethanol: water (7:3, v/v) and their in vitro antibacterial activity evaluated through both the agar diffusion and broth microdilution methods against 60 Oxacillin Resistant S. aureus (ORSA) strains and against S. aureus ATCC6538. The extracts from B. pilosa and A. crassiflora inhibited the growth of the ORSA isolates in both methods. Leaves of B. pilosa presented mean of the inhibition zone diameters significantly higher than chlorexidine 0.12% against ORSA, and the extracts were more active against S. aureus ATCC (p < 0.05). Parallel, toxicity testing by using MTT method and phytochemical screening were assessed, and three extracts (B. pilosa, root and leaf, and A. crassiflora, seed) did not evidence toxicity. On the other hand, the cytotoxic concentrations (CC50 and CC90) for other extracts ranged from 2.06 to 10.77 mg/mL. The presence of variable alkaloids, flavonoids, tannins and saponins was observed, even though there was a total absence of anthraquinones. Thus, the extracts from the leaves of B. pilosa revealed good anti-ORSA activity and did not exhibit toxicity.

scholarly journals Laboratory germination testing of the sweet chestnut (Castanea sativa Mill.) according to ISTA rules

2019 ◽  
Vol 143 (9-10) ◽  
pp. 469-477
Author(s):  
Damird Drvodelić ◽  
Igor Poljak ◽  
Marilena Idžojtić ◽  
Ivana Zegnal ◽  
Katarina Tumpa ◽  
...  
Keyword(s):  
Castanea Sativa ◽  
Sweet Chestnut ◽  
Seed Testing ◽  
Castanea Sativa Mill

U radu su prikazani rezultati ispitivanja laboratorijske klijavosti i morfoloških značajki klijanaca europskoga pitomoga kestena (Castanea sativa Mill.). Uzorci za istraživanja sakupljeni su u submediteranskom području Hrvatske, a radni uzorak za ispitivanje klijavosti iznosio je 8×25 sjemenki. Sjeme je do ispitivanja klijavosti čuvano tri mjeseca u hladnjaku na temperaturi od 3 °C. Postotak laboratorijske klijavosti utvrđen je prema postotku pravilnih klijanaca koji su normalno proklijali nakon 27 dana ispitivanja. Ispitivanje je provedeno u laboratoriju prema uvjetima koje propisuje ISTA (International Seed Testing Association). Izrađen je katalog sa slikama i opisom nepravilnih klijanaca. Na osnovi definiranih kodova nepravilnih klijanaca moguće je preventivno djelovati kako bi se reducirale nepoželjne jedinke. Rezultati ovog istraživanja od velike su koristi za razumijevanje složenoga procesa klijanja sjemena, kako u laboratoriju, tako i šumskim rasadnicima.

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