scholarly journals Inactivated vaccine against Aujeszky's disease

2021 ◽  
pp. 2957-2963
Author(s):  
Zhanat B. Kondibaeva ◽  
Bolat A. Yespembetov ◽  
Khairulla B. Abeuov ◽  
Assiya K. Mussayeva ◽  
Sarsenbek T. Siyabekov ◽  
...  
Keyword(s):  
Cell Culture ◽  
Virus Strain ◽  
Reaction Medium ◽  
Inactivated Vaccine ◽  
Medium Temperature ◽  
Infectious Dose ◽  
Aujeszky's Disease ◽  
Aujeszky’S Disease ◽  
The Central Nervous System ◽  
Ph Neutralization

Background and Aim: The Aujeszky's disease, also known as Pseudorabies, remains one of the most problematic fulminant diseases in domestic animals, affecting the central nervous system. The study aimed to investigate the effect of an inactivated vaccine against Aujeszky's disease based on "Kordai" virus strain. Materials and Methods: To test the inactivation of the "Kordai" strain (grown by the roller method in VNK-21/13 cell culture with an infectious titer of at least 7.5 lg TCD50/ml) which is causative of Aujeszky's disease, next-generation teotropin and propolis preparations were used in concentrations of 0.1%, 0.08%, and 0.04%. Results: As a result of comparative studies on the optimization of parameters for inactivating the "Kordai" virus strain, it was established that teotropin is a more effective inactivant than propolis. At the same time, the optimal final concentration of teotropin for inactivation was 0.1%, along with a reaction medium temperature of 37°C, pH of 7.4-7.6, and duration of inactivation of 14 h. The titer of virus-neutralizing activity (VNA) of antibodies at the pH (neutralization reactions) in vaccinated sheep of 10-12 months of age was 7.5±0.3, Ig TCID50/ml (tissue culture infectious dose 50%), and 3.5±0.3 in the cell culture VNK-21/13 (culture of Syrian hamster kidney cells). Conclusion: To determine colostral immunity in newborn lambs, the method of metabolic status correction was used to vaccinate lambs obtained from immune sheep 4 months after birth. The results showed that lambs obtained from immune sheep had high VNA titers. A sustained immune response in vaccinated animals was obtained after double vaccination.

scholarly journals Modification of a strain of Aujeszky's disease virus in cell culture

1977 ◽  
Vol 26 (3) ◽  
pp. 453-453 ◽  
Author(s):  
B. TOMA ◽  
A. BRUN ◽  
P. VANNIER ◽  
G. CHAPPUIS
Keyword(s):  
Cell Culture ◽  
Disease Virus ◽  
Aujeszky's Disease ◽  
Aujeszky’S Disease ◽  
Aujeszky’S Disease Virus

scholarly journals TESTING OF COMBINED VACCINES AGAINST AUJEZSKY’S DISEASE, PORCINE REPRODUCTIVE AND RESPIRATORY SYNDROME AND PORCINE PARVOVIRUS INFECTION FOR THEIR ANTIGENICITY

2018 ◽  
pp. 13-17
Author(s):  
Ye. P. Baborenko ◽  
Ye. K. Dolganova ◽  
K. N. Gruzdev
Keyword(s):  
Disease Virus ◽  
Virus Strain ◽  
Porcine Parvovirus ◽  
Prrs Virus ◽  
Aujeszky's Disease ◽  
Aujeszky’S Disease ◽  
Aujeszky’S Disease Virus ◽  
Pig Farm ◽  
The Russian Federation ◽  
Control And Eradication

Tests of inactivated emulsion anti-Aujezsky’s disease, porcine reproductive and respiratory syndrome (PRRS) vaccine based on marker Aujezsky’s disease virus strain, PRRS virus strain and inactivated emulsion anti-Aujezsky’s disease and porcine parvovirus infection vaccine based on marker Aujezsky’s disease virus strain and porcine parvovirus strain developed by the FGBI “ARRIAH” for their antigenicity are described in the paper. It is demonstrated that the said vaccines can be used successfully for PRRS and porcine parvovirus infection and Aujezsky’s disease prevention. They are becoming of special significance when used in programs on Aujeszky’s disease control and eradication. The use of the vaccine based on marker Aujeszky’s disease virus strain allows differentiation between the animals that have antibodies to the marker vaccine strain (in the absence of antibodies to the glycoprotein gE in 100% of cases) from the animals with antibodies to field strains of the Aujeszky’s disease virus in their sera. A comprehensive assessment of the use of associated vaccines developed in the FGBI “ARRIAH” (against Aujeszky’s disease based on the marker strain, reproductive and respiratory syndrome, parvovirus infection), in the framework of anti-epidemic measures on a pig farm of the Russian Federation showed their safety, high antigenicity, immunogenicity.

Use of immunoenzyme technique for the detection of Aujeszky's disease virus in cell culture

1978 ◽  
Vol 102 (21) ◽  
pp. 462-463 ◽  
Author(s):  
J. Roszkowski ◽  
M. Bartoszcze ◽  
J. Zadura ◽  
Z. Swiatek
Keyword(s):  
Cell Culture ◽  
Disease Virus ◽  
Aujeszky's Disease ◽  
Aujeszky’S Disease ◽  
Aujeszky’S Disease Virus ◽  
Immunoenzyme Technique

scholarly journals Aujeszky's Disease (Pseudorabies). An old threat in current pig industry? Part I. Pathogenetic information and implications

2017 ◽  
Vol 62 (1) ◽  
pp. 29 ◽  
Author(s):  
K. V. PAPAGEORGIOU (Κ.Β. ΠΑΠΑΓΕΩΡΓΙΟΥ) ◽  
A. R. BURRIEL ◽  
G. FILIOUSSIS (Γ. ΦΙΛΙΟΥΣΗΣ) ◽  
V. PSYCHAS (Β. ΨΥΧΑΣ) ◽  
H. NAUWYNCK ◽  
...  
Keyword(s):  
Viral Disease ◽  
Upper Respiratory Tract ◽  
Reproductive Disorders ◽  
Respiratory Problems ◽  
Aujeszky's Disease ◽  
Aujeszky’S Disease ◽  
Replication Sites ◽  
The Central Nervous System ◽  
Characteristic Features ◽  
Cellular Components

Aujeszky's disease (AD) (or Pseudorabies) is an important viral disease of swine causing neurological signs in neonatal pigs, respiratory problems in fatteners and reproductive disorders in breeding stock. Glycoproteins and enzymes of the AD virus (ADV) and their interactions with the cellular components majorly determine its pathogenesis. After primary replication in the epithelia of the upper respiratory tract, ADV travels via the nerves to the central nervous system and via the blood to secondary replication sites (lymph nodes, lungs, uterus etc). Depending on the age of the affected swine, it can cause neonatal mortality due to neurological disease, respiratory disease in growing and adult pigs and reproductive disorders in breeding animals. One of the characteristic features of ADV is latency. In this paper, its major points on etiology, pathogenesis, clinical and post mortem findings and diagnosis are presented.

scholarly journals First Report of a Severe Outbreak of Aujeszky’s Disease in Cattle in Sicily (Italy)

Pathogens ◽  
2020 ◽  
Vol 9 (11) ◽  
pp. 954
Author(s):  
Flavia Pruiti Ciarello ◽  
Maria Teresa Capucchio ◽  
Dorotea Ippolito ◽  
Elena Colombino ◽  
Lucia Rita Maria Gibelli ◽  
...  
Keyword(s):  
Natural Infection ◽  
Wild Strain ◽  
Clinical Signs ◽  
Cutaneous Lesions ◽  
Indirect Contact ◽  
System Diagnosis ◽  
Aujeszky's Disease ◽  
Aujeszky’S Disease ◽  
The Central Nervous System ◽  
Serological Data

Aujeszky’s disease in cattle is caused by Suid herpes virus 1. The natural infection has been reported worldwide in bovine species and it is related to direct and indirect contact with infected pigs, which represent the main reservoir of the virus. Here, it is reported the first documented outbreak of Aujeszky’s disease in cattle in Sicily (Italy). Severe itching and nonspecific neurological symptoms were the main reported clinical signs. No characteristic gross and histological features were reported other than cutaneous lesions caused by excessive pruritus and hyperaemia, haemorrhages and inflammation in the central nervous system. Diagnosis was confirmed by real time PCR and immunohistochemistry on the nervous tissue. The route of infection remained unknown, but serological data observed in pigs living in close cohabitation with cattle revealed a circulation of a wild strain of the virus in the area. This study contributes to a better knowledge of this disease in a non-conventional host and suggests the need to increase the prophylaxis control plans in specific breeding contexts.

scholarly journals Infection of fattening pigs by a virulent strain of Aujeszky's disease virus and its spread in the pig

2018 ◽  
Vol 50 (4) ◽  
pp. 310
Author(s):  
S. K. KRITAS (Σ.Κ. ΚΡΗΤΑΣ) ◽  
H. J. NAUWYNCK ◽  
M. B. PENSAERT ◽  
S. C. KYRIAKIS (Σ.Κ. ΚΥΡΙΑΚΗΣ)
Keyword(s):  
Disease Virus ◽  
Virulent Strain ◽  
Post Inoculation ◽  
Replication Rate ◽  
Aujeszky's Disease ◽  
Aujeszky’S Disease ◽  
Aujeszky’S Disease Virus ◽  
Pulmonary Macrophages ◽  
The Central Nervous System ◽  
Fattening Pigs

In this paper, the spread of the virulent 75V19 strain of Aujeszky's disease virus (ADV) in fattening pigs has been examined, and the association of this parameter to the development of relative symptoms has being sought. Four fattening pigs without antibodies against ADV have been oronasally inoculated by instillation of 1075 TCID50 of the infective strain, whilst another four seronegative pigs have been infected by the same dose of the strain administered in aerosol form. The pigs were euthanised at 4 days post inoculation and their tissues were examined by virus isolation and quantification in cell cultures. An extended spread of the virus was seen with both inoculation methods. However, the nasopharyngeal mucosa, the diaphragmatic lobes of the lungs and the pulmonary macrophages were more extensively infected in aerosol inoculated pigs compared to those of the other group. No difference was observed as regards the appearance of clinical disease between the two groups of fattening pigs. The results have also shown that, despite the high replication rate of ADV in the nasal mucosa and its ability to invade the central nervous system of fattening pigs, the degree of its replication in the nervous tissue in these pigs is limited when compared to suckling pigs.

Sign in / Sign up

Export Citation Format

Share Document