Physico-Chemical Studies of Chitosan Derivatives and
Optimization of Reaction Conditions using RSM Design

In this work, the chitosan flake was converted to carboxymethyl chitosan (CMCS) by carboxymethylation process using monochloroacetic acid in alkaline condition. The structure of carboxymethyl chitosan was confirmed by NMR, FT-IR, XRD, TGA and SEM techniques. The average molecular weight of carboxymethyl chitosan was obtained by viscometry method. The carboxymethylation reaction of chitosan was optimized for the reaction time, reaction temperature, amount of chitosan, amount of monochloroacetic acid. Response surface methodology (RSM) was used to analyze degree of substitution, degree of acetylation and yield with respect to reaction conditions. At the optimization reaction condition the degree of substitution, degree of acetylation (%) and yield (%) values are 0.56, 33.99 and 78.2, respectively.

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1H-NMR Analysis of Degree of Substitution in N,O-Carboxymethyl Chitosans from Various Chitosan Sources and Types

Advanced Materials Research ◽

10.4028/www.scientific.net/amr.506.158 ◽

2012 ◽

Vol 506 ◽

pp. 158-161 ◽

Cited By ~ 5

Author(s):

A. Jaidee ◽

Pornchai Rachtanapun ◽

S. Luangkamin

Keyword(s):

Chemical Structure ◽

Carboxymethyl Chitosan ◽

Degree Of Substitution ◽

Resonance Spectroscopy ◽

Nmr Analysis ◽

Reaction Conditions ◽

H Nmr ◽

Fourier Transformed Infrared Spectroscopy ◽

Chitosan Oligomer ◽

Ft Ir

N,O-Carboxymethyl chitosans were synthesized by the reaction between shrimp, crab and squid chitosans with monochloroacetic acid under basic conditions at 50°C. The mole ratio of reactants was obtained from various reaction conditions of shrimp chitosan polymer and oligomer types. The mole ratio 1:12:6 of chitosan:sodium hydroxide:monochloroacetic acid was used for preparing carboxymethyl of chitosan polymer types while carboxymethyl of chitosan oligomer types were used the mole ratio 1:6:3 of chitosan:sodium hydroxide:monochloroacetic acid. The chemical structure was analyzed by fourier transformed infrared spectroscopy (FT-IR) and proton nuclear magnatic resonance spectroscopy (1H-NMR). The FT-IR was used for confirm the insertion of carboxymethyl group on chitosan molecules. The 1H-NMR was used for determining the degree of substitution (DS) of carboxymethylation at hydroxyl and amino sites of chitosans. Carboxymethyl chitosan samples had the total DS of carboxymethylation ranging from 1.0-2.2. The highest of DS of carboxymethylation was from shrimp chitosan oligomer type.

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Chitosan and Carboxymethyl Chitosan from Fish Scales of Labeo rohita

Dhaka University Journal of Science ◽

10.3329/dujs.v61i1.15116 ◽

2013 ◽

Vol 61 (1) ◽

pp. 145-148 ◽

Cited By ~ 6

Author(s):

Tanvir Muslim ◽

Mohammad Habibur Rahman ◽

HOsne Ara Begum ◽

Md. Azizur Rahman

Keyword(s):

Molecular Weight ◽

Spectral Analysis ◽

Labeo Rohita ◽

Carboxymethyl Chitosan ◽

Degree Of Substitution ◽

Monochloroacetic Acid ◽

Fish Scales ◽

Ft Ir

Chitin was extracted from the fish scales of Labeo rohita and chitosan was successfully prepared from it by deacetylation reaction. The prepared chtiosan was characterized by FT-IR spectral analysis and degree of decetylation was determined by pH-metric titration. The molecular weight of chitosan was estimated by viscometric method. Chitosan was converted into its carboxymethyl derivative using alkali and monochloroacetic acid. The prepared carboxymethyl chitosan was characterized by FT-IR spectral analysis and degree of substitution was estimated. Dhaka Univ. J. Sci. 61(1): 145-148, 2013 (January) DOI: http://dx.doi.org/10.3329/dujs.v61i1.15116

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Developm ent of carboxymethylcellulose from nata de coco

ASEAN Journal on Science and Technology for Development ◽

10.29037/ajstd.307 ◽

2017 ◽

Vol 18 (2) ◽

Author(s):

Lydia S. Manguiat, ◽

Veronica C. Sabularse, and ◽

Dario C. Sabularse Sabularse

Keyword(s):

Chemical Properties ◽

Degree Of Substitution ◽

Raw Material ◽

Monochloroacetic Acid ◽

Processing Conditions ◽

Food Grade ◽

Degree Of Polymerisation ◽

Physico Chemical

Nata de coco was used as raw material for the preparation of food-grade carboxymethylcellulose referred to as carboxymethyl-nata (CMN). The effect of various conditions in the carboxymethylation of nata cellulose was determined. The concentration of NaOH, mercerisation time, shredding time during mercerisation and etherification, and the concentration of monochloroacetic acid significantly affected the following physico-chemical properties of CMN, namely, degree of substitution (DS), viscosity, degree of polymerisation (DP), and solubility. The best processing conditions were: the use of 40% NaOH, 60 mins mercerisation time, 60 mins shredding time during mercerisation and etherification, and J.35g/mL ClCH COOH. CMN with desirable properties wasproduced using these conditions.

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