The Effect of Light Quality on the Content of Photosynthetic Pigments in Potato (Solanum tuberosum L.) in vitro

2014 ◽  
Author(s):  
Elena Myakisheva ◽  
Galina Sokolova
Keyword(s):  
Solanum Tuberosum ◽  
Photosynthetic Pigments ◽  
Light Quality ◽  
Solanum Tuberosum L ◽  
Effect Of Light

scholarly journals EFFECT OF LIGHT, CO2, AND SUCROSE CONCENTRATIONS ON THE IN VITRO GROWTH OF WHITE POTATO (SOLANUM TUBEROSUM L.)

HortScience ◽  
1992 ◽  
Vol 27 (6) ◽  
pp. 696f-696
Author(s):  
N.C. Yorio ◽  
R.M. Wheeler ◽  
R.C. Weigel
Keyword(s):  
Solanum Tuberosum ◽  
Solanum Tuberosum L ◽  
Dry Weight ◽  
Growth Chamber ◽  
Photon Flux ◽  
Photosynthetic Photon Flux ◽  
White Potato ◽  
High Sucrose ◽  
Effect Of Light

Growth measurements of potato (Solanum tuberosum L.) cvs. Norland (NL), Denali (DN), and Kennebec (KN) were taken from 21-day-old plantlets grown in vitro. Studies were conducted in a growth chamber, with nodal explants grown in culture tubes with loose-fitted Magenta 2-way caps containing Murashige and Skoog salts with either 0, 1, 2 or 3% sucrose. The cultures received either 100 or 300 μmol m-2 s-1 photosynthetic photon flux (PPF), and the growth chamber was maintained at either 400 or 4000 μmol mol-1 CO2. All cvs. showed significant increases in growth on 0% sucrose media at 4000 μmol mol-1 CO2, indicating an autotrophic response. At 400 μmol mol-1 CO2, all cvs. showed an increase in total plantlet dry weight (DW) with increasing sucrose under both PPF levels. Within any sucrose treatment, the highest total DW for all cvs. resulted from 300 μmol m-2 s-1 PPF and 4000 μmol mol-1 CO2. At 4000 μmol mol-1 CO2, shoot DW declined with sucrose above 2% for DN and sucrose above 1% for NL at both PPF levels, suggesting that high sucrose levels may hinder growth when CO2 enrichment is used.

scholarly journals Light Quality Affects Growth and Physiology of Carpesium triste Maxim. Cultured In Vitro

Agriculture ◽  
2020 ◽  
Vol 10 (7) ◽  
pp. 258
Author(s):  
Jin Zhao ◽  
Luc The Thi ◽  
Yoo Gyeong Park ◽  
Byoung Ryong Jeong
Keyword(s):  
Photosynthetic Pigments ◽  
Light Quality ◽  
Radical Scavenging ◽  
Photosynthetic Electron Transport ◽  
Total Flavonoids ◽  
Light Emitting ◽  
Radical Scavenging Capacity ◽  
Scavenging Capacity ◽  
Effect Of Light

The objective of this study was to investigate the effect of light quality on the growth and contents of photosynthetic pigments and total flavonoids, the parameters of chlorophyll a fluorescence, antioxidant capacities, and enzyme activities. It was intended to preliminarily explore the internal mechanisms involved for the effect of light quality on the growth and physiology of Carpesium triste Maxim. Stem apex explants were cultured on the Murashige and Skoog (MS) medium under white (W), red (R), blue (B), or a 1:1 mixture of red and blue (RB) light-emitting diodes (LEDs) for four weeks. The RB induced sturdy plantlets and increased the contents of photosynthetic pigments and total flavonoids, photosynthetic electron transport and efficiency, 2-diphenyl-1-picrylhydrazyl (DPPH) free radical scavenging capacity, and activities of antioxidant enzymes in the plantlets. Taken together, combinations of monochromatic red and blue LEDs could be used for the best production of high-quality C. triste plantlets in vitro.

Respon Pemberian Aspirin dan Kinetin Terhadap Pembentukan Umbi Mikro Kentang (Solanum tuberosum L.) Secara In Vitro

2017 ◽  
Author(s):  
Yunita Prameswari ◽  
FNU Djenal ◽  
FNU Damanhuri
Keyword(s):  
Solanum Tuberosum ◽  
Solanum Tuberosum L

Kebutuhan kentang yang semakin tinggi menyebabkan permintaan semakin meningkat. Rendahnya produksi kentang mengakibatkan berbagai upaya untuk peningkatan produksi terus dilakukan. Penggunaan metode kultur jaringan yaitu metode untuk mengisolasi bagian tanaman seperti protoplasma, sel, sekelompok sel, jaringan dan organ dalam kondisi aseptik, sehingga dapat diperbanyak dan beregenerasi menjadi tanaman utuh dapat dijadikan alternatif pemenuhan kebutuhan. Penelitian ini bertujuan untuk mengetahui kecepatan pembentukan umbi mikro kentang (Solanum Tuberosum L.) varietas granola kembang secarain vitro dengan menggunakan dua faktor dan 3 kali ulangan. Faktor pertama yaitu aspirin dengan tiga taraf (5,10,15) ppm. Faktor kedua yaitu kinetin dalam tiga taraf (8,10,12) ppm. Penelitian menggunakan seluruh propagul kentang yang berumur 30 hari setelah subkultur dan data yang didapat dianalisis menggunakan ANOVA. Hasil penelitian menunjukan bahwa interaksi aspirin dan kinetin tidak berpengaruh terhadap jumlah akar, kedinian umbi, dan bobot umbi. Interaksi perlakuan terbaik bagi pembentukan tunas yaitu A2K1 aspirin 10 ppm dan kinetin 8 ppm sedangkan Interaksi perlakuan terbaik pada parameter jumlah umbi yaituA3K2 aspirin 15 ppm dan kinetin 10.

Uji Toksisitas Beberapa Fungisida Nabati terhadap Penyakit Layu Fusarium (Fusarium oxysporum) pada Tanaman Kentang (Solanum tuberosum L.) secara In Vitro (Toxicity Test of several Biofungicides in controlling Fusarium wilt (Fusarium oxysporum) in Potato Plants (Solanum tuberosum L.) by In Vitro)

2019 ◽  
Vol 9 (2) ◽  
pp. 91
Author(s):  
Ghea Dotulong ◽  
Stella Umboh ◽  
Johanis Pelealu
Keyword(s):  
Solanum Tuberosum ◽  
Fusarium Oxysporum ◽  
Fusarium Wilt ◽  
Leaf Extract ◽  
Solanum Tuberosum L ◽  
In Vitro Toxicity ◽  
Potato Plants ◽  
Colony Diameter ◽  
In Vitro Toxicity Test

Uji Toksisitas Beberapa Fungisida Nabati terhadap Penyakit Layu Fusarium (Fusarium oxysporum) pada Tanaman Kentang (Solanum tuberosum L.) secara In Vitro (Toxicity Test of several Biofungicides in controlling Fusarium wilt (Fusarium oxysporum) in Potato Plants (Solanum tuberosum L.) by In Vitro) Ghea Dotulong1*), Stella Umboh1), Johanis Pelealu1), 1) Program Studi Biologi, FMIPA Universitas Sam Ratulangi, Manado 95115*Email korespondensi: [email protected] Diterima 9 Juli 2019, diterima untuk dipublikasi 10 Agustus 2019 Abstrak Tanaman kentang (Solanum tuberosum L.) adalah salah satu tanaman hortikultura yang sering mengalami penurunan dari segi produksi dan produktivitasnya, akibat adanya serangan penyakit layu yang salah satunya disebabkan oleh Fusarium oxysporum. Tujuan penelitian ini adalah mengidentifikasi toksisitas beberapa fungisida nabati dalam mengendalikan penyakit Layu Fusarium (F. oxysporum) pada tanaman kentang (Solanum tuberosum L.) secara In Vitro. Metode Penelitian yang digunakan yaitu metode umpan beracun. Data dianalisis dengan Rancangan Acak Lengkap (RAL) dengan Analisis Varian (ANAVA) yang dilanjutkan dengan menggunakan metode BNT (Beda Nyata Terkecil). Hasil Penelitian, diperoleh nilai toksisitas fungisida nabati tertinggi yaitu pada ekstrak daun sirsak dengan nilai HR (69,44%), kategori berpengaruh, ditandai dengan diameter koloni 2,75 cm (100ppm) dan yang terendah toksisitasnya yaitu pada ekstrak daun jeruk purut dengan nilai HR (49,81%), kategori cukup berpengaruh ditandai dengan diameter koloni 3,75 cm (25ppm). Semakin tinggi konsentrasi yang diujikan maka semakin tinggi toksisitas dari fungisida nabati yang diberikan.Kata Kunci: fungisida nabati, Fusarium oxysporum, tanaman kentang, In Vitro Abstract Potato plants (Solanum tuberosum L.) is one of the horticulture plants which often decreases in terms of production and productivity, due to the attack of wilt, one of which is caused by Fusarium oxysporum. The purpose of this study was to determine the toxicity of several biofungicides in controlling Fusarium wilt (F. oxysporum) in potato plants (Solanum tuberosum L.) in Vitro. The research method used was the In Vitro method with the poison bait method. Data were analyzed by Completely Randomized Design with Variant Analysis (ANAVA), followed by the BNT method. The results showed that the highest biofungicide toxicity value was soursop leaf extract with HR values (69.44%), influential categories, characterized by colony diameter 2.75 cm (100ppm) and the lowest toxicity, namely in kaffir lime leaf extract with a value of HR (49.81%), quite influential category was characterized by colony diameter of 3.75 cm (25ppm). The higher the concentration tested, the higher the toxicity of the biofungicide given.Keywords: biofungicides, Fusarium oxysporum, Potato Plants, In Vitro.

Оценка влияния отдельных факторов на производство безвирусных мини-клубней картофеля

2021 ◽  
pp. 3-7
Author(s):  
Елена Васильевна Шищенко ◽  
Елена Николаевна Барсукова
Keyword(s):  
Solanum Tuberosum ◽  
Solanum Tuberosum L

Одним из этапов технологического процесса производства оригинального семенного картофеля является выращивание оздоровленных пробирочных микрорастений в весенне-летней теплице для получения мини-клубней. Исследования проводили в Приморском крае на оздоровленных безвирусных пробирочных растениях картофеля (Solanum tuberosum L.) сорта Метеор, размноженных методом микрочеренкования в культуре in vitro. Данный сорт был выбран вследствие его скороспелости. В ходе эксперимента при выращивании микрорастений изучено влияние сочетания факторов — объем пробирок, состав почвенного грунта и площадь питания растения на производство мини-клубней картофеля в условиях искусственного освещения. Дана оценка продуктивности пробирочных растений и определена роль основного фактора, влияющего на выход мини-клубней картофеля сорта Метеор, в лабораторных условиях. Установлено, что основное влияние на производство стандартных мини-клубней картофеля оказал состав почвенного грунта. Максимальное количество стандартных мини-клубней картофеля сорта Метеор (209,3 и 156,1 шт./м2) получено в вариантах опыта при использовании грунта с содержанием элементов питания: фосфора — 1380 мг/кг, калия — 1425 мг/кг, азота — 1394 мг/кг, органического вещества — 66,7 %.

Sign in / Sign up

Export Citation Format

Share Document