scholarly journals In vivo and in vitro propagation of “macela”: a medicinal-aromatic native plant with ornamental potential

2018 ◽  
Vol 24 (4) ◽  
pp. 361-370
Author(s):  
Julián Guariniello ◽  
Jésica Iannicelli ◽  
Patricia Angélica Peralta ◽  
Alejandro Salvio Escandón
Keyword(s):  
De Novo ◽  
Basal Medium ◽  
Nodal Segments ◽  
Naphthalene Acetic Acid ◽  
Multiplication Rate ◽  
Ms Medium ◽  
Native Plant ◽  
Popular Medicine

Achyrocline satureioides is a shrub native from South America. In popular medicine it is used in infusions such as digestive, carminative, antispasmodic, eupeptic and emmenagogue. However, its main use is as an ingredient in the liquor industry. Commercial exploitation is carried out through the collection of natural populations in an unsustainable way. The micropropagation of A. satureioides will allow its massive propagation and it will settle a base for its domestication. For this, a clone denominated as M1-5 was first propagated by cuttings. Subsequently, nodal segments obtained from young stems were disinfected by a standard method and cultured on MS medium. These shoots were used as a source of explants for subsequent assays. For its in vitro establishment MS medium and WPM were tested. Once the culture was established, the responses of the explants to increasing concentrations of 6-benzylaminopurine (BAP) (0.0; 0.5; 2.5 and 5.0 μM) with and without 0.05 μM α-naphthalene acetic acid (NAA) on WPM as basal medium were studied during 35 days. The proliferation of buds, the presence of callus and the number and length of the roots were evaluated. All of the “macela” cuttings in vivo propagated rooted and developed satisfactorily under the conditions tested. The application of 5.0 μM BAP alone generated the best multiplication rate, so it was selected as the multiplication medium. De novo shoots rooted spontaneously and finally, transferred to the greenhouse. Here in it was possible to establish a micropropagation protocol not only for the production of plantlets of selected clones but also for the application of biotechnological tools in the development of A. satureioides germplasm.

scholarly journals Improved micropropagation and foliar micromorphological studies in Turnera ulmifolia L. – An important medicinal plant

2018 ◽  
Vol 30 (2) ◽  
pp. 283-294 ◽  
Author(s):  
Mani Manokari ◽  
Mahipal S. Shekhawat
Keyword(s):  
Shoot Proliferation ◽  
Nodal Segments ◽  
Naphthalene Acetic Acid ◽  
Ms Medium ◽  
Half Strength ◽  
Turnera Ulmifolia ◽  
Semi Solid ◽  
Number Of Shoots

Abstract The present study reports an efficient in vitro propagation system for Turnera ulmifolia using nodal segments as explants. Turnera ulmifolia (Passifloraceae) is an important garden plant with multipotent medicinal values. Effective shoot proliferation was achieved on agar gelled MS medium (Murashige and Skoog, 1962). The maximum number of shoots (8.3 ± 0.57) per initial explant was obtained on MS medium supplemented with 8.88 mM of 6-benzylaminopurine (BAP) and 0.54 mM of α-naphthalene acetic acid (NAA). The highest number of shoots (59.5 ± 2.10) proliferated on semi-solid MS medium (with agar) augmented with 2.22 mM of BAP and 2.32 mM of kinetin (Kin) along with 0.54 mM of NAA. Longer (4-5 cm) and healthy shoots were rooted (12.0 ± 0.10 roots per shoot) on half-strength MS medium fortified with 9.84 mM of indole-3 butyric acid (IBA). The in vitro regenerated plantlets were hardened in the greenhouse and transferred to the field. Significant developmental changes were observed in the foliar micromorphology of in vitro raised plantlets when these were transferred to the field. The stomatal index was gradually reduced (26.72 to 21.25) in the leaves from in vitro to field environments. But, vein-islets and veinlet terminations (13.4 and 7.6) were increased (39.7 and 18.4) respectively from in vitro to in vivo grown plants. Simple, unicellular, less frequent and underdeveloped trichomes were observed with the leaves of in vitro plants but fully developed trichomes recorded in the field transferred plants. The study could help in understanding the response and adaptation of tissue culture raised plantlets towards changed environmental conditions.

Morphological evaluation and antioxidant activity of in vitro- and in vivo-derived Echinacea purpurea plants

2012 ◽  
Vol 7 (4) ◽  
pp. 698-707 ◽  
Author(s):  
Ely Zayova ◽  
Ira Stancheva ◽  
Maria Geneva ◽  
Maria Petrova ◽  
Rumiana Vasilevska-Ivanova
Keyword(s):  
Acetic Acid ◽  
Basal Medium ◽  
Echinacea Purpurea ◽  
Water Soluble ◽  
Cultivated Plants ◽  
Naphthalene Acetic Acid ◽  
High Survival ◽  
Ms Medium

AbstractAn effective in vitro protocol for rapid clonal propagation of Echinacea purpurea (L.) Moench through tissue culture was described. The in vitro propagation procedure consisted of four stages: 1) an initial stage - obtaining seedlings on Murashige and Skoog (MS) basal medium with 0.1 mg L−1 6-benzylaminopurine, 0.1 mg L−1 α-naphthalene acetic acid and 0.2 mg L−1 gibberellic acid; 2) a propagation stage — shoot formation on MS medium supplemented with 1 mg L−1 6-benzylaminopurine alone resulted in 9.8 shoots per explant and in combination with 0.1 mg L−1 α-naphthalene acetic acid resulted in 16.2 shoots per explant; 3) rooting stage — shoot rooting on half strength MS medium with 0.1 mg L−1 indole-3-butyric acid resulted in 90% rooted microplants; 4) ex vitro acclimatization of plants. The mix of peat and perlite was the most suitable planting substrate for hardening and ensured high survival frequency of propagated plants. Significant higher levels were observed regarding water-soluble and lipid-soluble antioxidant capacities (expressed as equivalents of ascorbate and α-tocopherol) and total pnenols content in extracts of Echinaceae flowers derived from in vitro propagated plants and adapted to field conditions in comparison with traditionally cultivated plants.

scholarly journals In vitro multiplication, micromorphological studies and ex vitro rooting of Hybanthus enneaspermus (L.) F. Muell. – a rare medicinal plant

2018 ◽  
Vol 77 (1) ◽  
pp. 80-87 ◽  
Author(s):  
Mahipal S. Shekhawat ◽  
M. Manokari
Keyword(s):  
Medicinal Plant ◽  
Large Scale ◽  
Nodal Segments ◽  
Ms Medium ◽  
Ex Vitro ◽  
Culture Bottle ◽  
Hybanthus Enneaspermus ◽  
Half Strength

AbstractHybanthus enneaspermusis a rare medicinal plant. We defined a protocol for micropropagation,ex vitrorooting of cloned shoots and their acclimatization. Surface-sterilized nodal segments were cultured on Murashige and Skoog (MS) medium with different concentrations of 6-benzylaminopurine (BAP) and kinetin (Kin). Medium supplemented with 1.5 mg L−1BAP was found optimum for shoot induction from the explants and 6.4±0.69 shoots were regenerated from each node with 97% response. Shoots were further proliferated maximally (228±10.3 shoots per culture bottle with 7.5±0.43 cm length) on MS medium augmented with 1.0 mg L−1each of BAP and Kin within 4–5 weeks. The shoots were rootedin vitroon half strength MS medium containing 2.0 mg L−1indole-3 butyric acid (IBA). The cloned shoots were pulse-treated with 300 mg L–1 of IBA and cultured on soilrite® in a greenhouse. About 96% of the IBA-pulsed shoots rootedex vitroin soilrite®, each shoot producing 12.5±0.54 roots with 5.1±0.62 cm length. Theex vitrorooted plantlets showed a better rate of survival (92%) in a field study thanin vitrorooted plantlets (86%). A comparative foliar micromorphological study ofH. enneaspermuswas conducted to understand the micromorphological changes during plant developmental processes fromin vitrotoin vivoconditions in terms of variations in stomata, vein structures and spacing, and trichomes. This is the first report onex vitrorooting inH. enneaspermusand the protocol can be exploited for conservation and large-scale propagation of this rare and medicinally important plant.

scholarly journals In vitro regeneration protocol for artificial seed production in an important medicinal plant Mentha arvensis L

2014 ◽  
Vol 20 ◽  
pp. 99-108 ◽  
Author(s):  
MS Islam ◽  
MA Bari
Keyword(s):  
Medicinal Plants ◽  
Seed Production ◽  
In Vitro Regeneration ◽  
Basal Medium ◽  
Nodal Segments ◽  
Ms Medium ◽  
Mentha Arvensis ◽  
Artificial Seed ◽  
Artificial Seed Production

Context: The application of encapsulated shoot tips and nodal segments may contribute to the protection of rare and threatened medicinal plants. Although the artificial seed technique has been reported for more than two decades, for medicinal plants this method has not been developed sufficiently. The main limitations in conventional propagation of some species with medicinal value are: reduced endosperm, low germination rate and seedless varieties. The above mentioned reasons indicate the need for the production of artificial seeds as a technique which combines the advantages of clonal multiplication with those of seed propagation and storage. Objectives: The objective of the present investigation was to standardize artificial seed production technology taking shoot tip and nodal explants in Mentha arvensis and its in vitro regeneration Materials and Methods: Sodium alginate beads were produced by encapsulation of shoot tip and nodal segments of the plant M. arvensis. MS medium was used as basal medium with agar and sodium alginate was used as gelling agent accompanied by CaCl2 solution. Results: Different concentrations and combinations of BAP, Kin and NAA were used in alginate bead in MS basal medium. Among the different concentrations of phytohormone, highest 80% of shoot formation was observed in MS medium containing 2.0 mg/l BAP + 0.2 mg/l NAA from nodal segments of M. arvensis. Highest average number of shoot 9.87 ± 0.58 formation was obtained in the same medium but highest length of shoot 6.27 ± 0.29 cm was found in the medium having 1.0 mg/l BAP + 0.5 mg/l NAA. Conclusion: The present investigation clearly established and demonstrated the method of obtaining the artificial seed production in M. arvensis supported by different hormone concentrations DOI: http://dx.doi.org/10.3329/jbs.v20i0.17722 J. bio-sci.  20:  99-108, 2012

scholarly journals In Vitro Long-Term Cultures of Papaya (Carica Papaya L.).

2021 ◽  
Author(s):  
Jose Javier Regalado González ◽  
Manuel López Granero ◽  
Carlos Lopez Encina
Keyword(s):  
Morphological Characteristics ◽  
Basal Medium ◽  
Multiplication Rate ◽  
Ms Medium ◽  
High Quality ◽  
Poor Growth ◽  
Average Multiplication ◽  
Half Strength

Abstract We present the data on proliferation corresponding to 10 years of continuous incubation in vitro of papaya shoots, and propose a reliable method for long-term micropropagation for papaya, using two types of explants: Microshoots from somatic embryos, and from axillary buds of papaya. Three different media were assayed. The proliferation medium (PPRM) allowed to maintain papaya shoots under continuous proliferation during 20 years, maintaining a consistent behaviour. Most of the shoots developed in PPRM rooted during the incubation, and after acclimated easily, maintaining the morphological characteristics of the parental plants, flowering and setting fruits normally. The PPRM medium consist in MS medium supplemented with NAA (0.1 mg l-1), BA (0.5 mg l-1), GA3 (0.5 mg l-1) and Adenine sulphate (40 mg l-1). The average multiplication rate was higher than 20 shoots per explant along the long-term assay. The elongation medium (PELM), was designed to recover shoots with a poor growth, and allowed the development of high quality shoots ready for rooting, and consist in a MS basal medium supplemented with NAA (0.1 mg l-1), Kin (0.5 mg l-1) and GA3 (1 mg l-1). The rooting medium (PROM) was designed to induce high quality roots from non-rooted shoots and consist in a half strength MS medium plus IBA (1mg l-1). On PROM, agar can be exchanged for expanded vermiculite. Acclimation took place inside an acclimatization tunnel under progressive hydric stress. After 4 weeks, the plant recovery rate was 90% for plants maintained under continuous proliferation during ten years.

scholarly journals In vitro seed germination in Cymbidium elegans Lindl. and Dendrobium densiflorum Lindl. ex Wall. (Orchidaceae)

2010 ◽  
Vol 6 ◽  
pp. 100-102 ◽  
Author(s):  
Shreeti Pradha ◽  
Bijaya Pant
Keyword(s):  
Seed Germination ◽  
Basal Medium ◽  
Naphthalene Acetic Acid ◽  
Genetic Constitution ◽  
Ms Medium ◽  
Orchid Species ◽  
C Elegans ◽  
Protocorm Formation ◽  
Dendrobium Densiflorum

A comparative study of in vitro seed germination of two endangered orchid species, viz. Cymbidium elegans Lindl. and Dendrobium densiflorum Lindl. ex Wall., was carried out on Murashige and Skoog's (MS) medium, supplemented with different concentrations and combination of 6-benzylaminopurine (BAP) and á-Naphthalene acetic acid (NAA). The hormone-free MS medium and MS medium supplemented with various growth hormones were found effective for in vitro seed germination of both species. However, the seeds of these two species showed variation in their germination behavior. Hormone-free MS basal medium was found most effective for seed germination of D. densiflorum; whereas, basal medium supplemented with BAP (1mg/l) was effective for C. elegans. The seeds of D. densiflorum showed quick response in earlier germination, protocorm formation and further development into seedlings in comparison to C. elegans. In C. elegans, germination of immature seeds started after nine weeks of inoculation; whereas in D. densiflorum, the initiation of germination started after five weeks of culture. The variations in seed germination, protocorm formation and seedling differentiation in the two orchid species might be due to the differences in their genetic constitution and the presence of different endogenous growth stimulating substances present in their seeds. The present study has provided useful information for in vitro clonal mass multiplication of these commercially important orchid species. Key-words: growth hormone; in vitro study; orchid.DOI: 10.3126/botor.v6i0.2917 Botanica Orientalis - Journal of Plant Science (2009) 6: 100-102

Micropropagation and In Vitro Inflorescence of Pentas lanceolata

2022 ◽  
Author(s):  
Kitti Bodhipadma ◽  
Sompoch Noichinda ◽  
Chutikarn Tangtivaporn ◽  
Saowaros Phanomchai ◽  
David W. M. Leung
Keyword(s):  
Basal Medium ◽  
Tubular Structure ◽  
Nodal Explants ◽  
Ms Medium ◽  
Water Accumulation ◽  
Semi Solid ◽  
Pentas Lanceolata

In this study, different concentrations of 6-benzyladenine (BA) on in vitro shoot and inflorescence inductions of P. lanceolata were investigated. The in vivo and in vitro floral characteristics of this plant were also compared. Nodal explants of P. lanceolata were cultured vertically with the cut ends inserted into semi-solid Murashige and Skoog (MS) medium supplemented with 0, 0.5, 1, 2, 4, and 8 mg L–1 BA. The results showed that the explants formed the highest numbers of shoots even when cultured in MS basal medium without any addition of BA, while the shoots formed in the explants cultured in MS medium supplemented with 1 mg L–1 BA were the longest. No inflorescence was found in the shoots cultured in MS medium supplemented with 8 mg L–1 BA, while the highest percentage of inflorescence induction was found in the shoots cultured in the medium supplemented with 0.5 mg L–1 BA. The apperances of in vivo and in vitro flowers of P. lanceolata were the same in many aspects except that the number of flower/inflorescence formed was different. In addition, water accumulation was observed only inside the in vitro flowers. Water deposit in the long tubular structure of P. lanceolata flower could cause anther injury, suggesting that flowers developed in vitro may not always produce pollen.

In vitro propagation of Camellia fascicularis: a plant species with extremely small populations

2020 ◽  
Vol 100 (2) ◽  
pp. 202-208
Author(s):  
Mengting Wang ◽  
Guiliang Zhang ◽  
Peiyao Xin ◽  
Yun Liu ◽  
Bin Li ◽  
...  
Keyword(s):  
Acetic Acid ◽  
Large Scale ◽  
In Vitro Regeneration ◽  
Germplasm Conservation ◽  
Shoot Multiplication ◽  
Shoot Tips ◽  
Naphthalene Acetic Acid ◽  
Multiplication Rate ◽  
Ms Medium

Camellia fascicularis is an endangered evergreen ornamental plant with pale yellow flowers. An efficient and reproducible in vitro regeneration method is required for its large-scale propagation and germplasm conservation. In this study, one axillary bud per nodal stem was obtained from C. fascicularis cultured on Murashige & Skoog (MS) medium containing 0.1 mg L−1 indole-3-acetic acid (IAA) combined with 1.0 mg L−1 6-benzylaminopurine (BA). Axillary buds from the stem segments were transferred to modified woody plant medium (WPM) supplemented with 3.0 mg L−1 BA in combination with 0.3 mg L−1 IAA for multiplication, thereby resulting in a high shoot multiplication rate of 6.8. Multiple shoots were divided into nodal stems and shoot tips and were induced to root. The shoot tips were induced to root by culturing on one-half MS medium supplemented with 2.0 mg L−1 indole-3-butyric acid (IBA) in combination with 0.3 mg L−1 α-naphthalene acetic acid (NAA), which resulted in 76.0% rooting efficiency with 2.3 roots per shoot. The optimal hormone ratio for inducing rooting of nodal stems was 1.0 mg L−1 IBA in combination with 2.0 mg L−1 NAA, which resulted in 72.7% rooting efficiency with 1.7 roots per nodal stem. These two rooted plantlets were successfully acclimatized and established in a greenhouse.

scholarly journals МОНГОЛ ОРНЫ ЭНДЕМИК УРГАМАЛ МОНГОЛ ДОГАР-CARYOPTERIS MONGOLICA BGE.-ИЙГ IN VITRO НӨХЦӨЛД ҮРЖҮҮЛСЭН ДҮНГЭЭС

2014 ◽  
Vol 52 (2) ◽  
pp. 23-28
Author(s):  
Д. Бямбасүх
Keyword(s):  
Acetic Acid ◽  
Basal Medium ◽  
Naphthalene Acetic Acid ◽  
Nodal Segment ◽  
Ms Medium ◽  
Endemic Plant ◽  
Shoot Height ◽  
Cotyledon Explants ◽  
Multiple Buds

Caryopteris mongolica Bge. буюу Монгол Догарын үрийг ариутгаад гиббереллиний хүчил(ГХ) 0.5мг/л, 1мг/л, 2мг/л тус тус агуулсан болон дан МС(хяналт) тэжээлт орчин дээр соёолуулсан. ГХ 2мг/л агуулсан хувилбар нь хяналттай харьцуулахад 20 хувиар үрийн соёололтыг нэмэгдүүлсэн. 28 хоногтой цухуйцаас хажуугийн нахиа бүхий ишний үе болон үрийн талын хэсгийг эксплант болгон сонгон авч Бензиламинопурин(БАП) 1мг/л, БАП 2мг/л ба Индол-3-цууны хүчил(ИЦХ) 0.3 мг/л, БАП 3мг/л концентрациар тус тус агуулсан, мөн Кинетин(КИН) 1мг/л, КИН 2мг/л ба α-нафталин цууны хүчил(НЦХ) 0.3мг/л, КИН 3мг/л ба НЦХ 0.4мг/л харьцаагаар тус тус агуулсан МС үндсэн тэжээлт орчинд өсгөвөрлөсөн. 21 хоногийн дараа найлзуур ургаж, нахиа олширсон байсан учир хэмжилт авч, 28 хоногийн дараа субкультур хийсэн. Нахиа үүсгэхэд хамгийн тохиромжтой орчны хувилбараар үрийн талын эксплант дээр 3 мг/л БАП, харин нахиа бүхий ишний үеийн эксплант дээр КИН=3мг/л, НЦХ=0.4мг/л өсөлтийн бодис агуулсан МС тэжээлт орчин байв. Үүссэн найлзууруудаа ямар нэг өсөлтийн бодис агуулаагүй ½ МС тэжээлт орчинд өсгөвөрлөхөд 7-10 хоногийн дараа үндэс үүсч эхэлж байсан бөгөөд 21-28 хоногт 95% нь үндэслэж байв.IN VITRO PROPAGATION THE MONGOLIAN ENDEMIC PLANT CARYOPTERIS MONGOLICA BGE.Caryopteris mongolica Bge. is rare and endemic plant of Mongolia, which used in traditional medicine of various diseases. We have initiated in vitro propagation the Caryopteris mongolica Bge. from seeds. Seeds were surface sterilized by immersing in 70% ethanol for 90 sec, 10% hydro peroxide for 10 minutes and 5% sodium hypochlorite for 20 minutes. Finally, rinsed with sterile distilled water for 4 times. Sterilized seeds were germinated on MS medium supplemented with 0.5, 1, 2 mg/l gibberillic acid, respectively. Nodal segment and cotyledon explants from 4 weeks old seedling were cultured on the MS basal medium supplemented with benzylaminopurine(BAP) 1 mg/l, BAP 2 mg/l and Indole acetic acid(IAA) 0.3 mg/l, BAP 3 mg/l, Kinetin(KIN) 1 mg/l, KIN 2 mg/l and Naphthalene acetic acid(NAA) 0.3 mg/l, KIN 3 mg/l and NAA 0.4 mg/l concentrations, respectively. After 3 weeks, measured of shoot height and bud number and 4 weeks later subcultured in a same medium. High frequency multiple buds were formed on MS medium supplemented with BAP 3mg/l, from cotyledon explants, but KIN 3 mg/l and NAA 0.4 mg/l was best version of nodal segment explants. All shoots were cultured in the ½ MS basal without any growth regulators for induction of root. After 7-10 days, initiation of root form and 21-28 days later 95% of cultured shoots were rooted.Key words: Caryopteris mongolica Bge., MS basal medium, Gibberillic acid, Benzylaminopurine, Kinetin, Indole-3-acetic acid, α-Naphthalene acetic acid, explants.DOI: http://dx.doi.org/10.5564/pmas.v52i2.356 Proceedings of the Mongolian Academy of Sciences Vol.52(2) 2012 p.23-28

scholarly journals Plant Regeneration from Leaf Explants of the Medicinal Herb Wedelia chinensis

Horticulturae ◽  
2021 ◽  
Vol 7 (10) ◽  
pp. 407
Author(s):  
Yung-Ting Tsai ◽  
Kin-Ying To
Keyword(s):  
Plant Regeneration ◽  
In Vitro Propagation ◽  
Liver Toxicity ◽  
Leaf Explants ◽  
Basal Medium ◽  
Nodal Segments ◽  
Naphthaleneacetic Acid ◽  
Ms Medium ◽  
Average Percentage

Wedelia chinensis, belonging to the Asteraceae family, has been used in folk medicine in East and South Asia for the treatment of common inflammatory diseases and protection against liver toxicity. Previously, in vitro propagation through different tissue explants has been reported, including through nodal segments, axillary buds, and shoot tips, whereas leaf segments failed to proliferate. Here, we report on the in vitro propagation of W. chinensis by culturing young leaf explants in MS medium supplemented with 0.5 mg/L α-naphthaleneacetic acid (NAA), 0.75 mg/L thidiazuron (TDZ), 1 mg/L gibberellic acid (GA3), 3.75 mg/L adenine, 3% sucrose, and 0.8% agar at pH 5.8. Calli were observed in all explants derived from the youngest top two leaves, and the average percentage of shoot regeneration was 23% from three independent experiments. Then, several shoots were excised, transferred onto MS basal medium supplemented with 3% sucrose and 0.8% agar at pH 5.8, and cultured in a growth chamber for 1 to 2 months. Roots were easily induced. Finally, plantlets carrying shoots and roots were transferred into soil, and all of them grew healthily in a greenhouse. No morphological variation was observed between the regenerated plantlets and the donor wild-type plants. In addition, we also established root cultures of W. chinensis in culture medium (MS medium, 3 mg/L NAA, 3% sucrose, pH 5.8) with or without 0.8% agar. To the best of our knowledge, this is the first paper reporting plant regeneration from leaf explants in the herbal plant W. chinensis.

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