scholarly journals Diagnostic value of islet autoantibody assays practised in Russia. 1. Classic immunofluorescence islet cell antibody assay, immunoradiometric glutamic acid decarboxylase antibody assay, and ELISA tyrosine phosphatase antibody and insulin antibody assays

2016 ◽  
Vol 19 (4) ◽  
pp. 331-340 ◽  
Author(s):  
Alexei V. Timofeev ◽  
Ksenia A. Gorst ◽  
Valentin Y. Uvarov ◽  
Ekaterina A. Pronina ◽  
Alisa V. Vitebskaya ◽  
...  
Keyword(s):  
Diagnostic Accuracy ◽  
Islet Cell ◽  
Tyrosine Phosphatase ◽  
Diagnostic Value ◽  
Islet Cell Antibody ◽  
Acid Decarboxylase ◽  
Antibody Assay ◽  
Glutamic Acid Decarboxylase Antibody ◽  
Cell Antibody

Objective. To estimate performance characteristics and diagnostic value of immunofluorescent islet cell antibody (ICA) assay, immunoradiometric glutamic acid decarboxylase antibody (GADA) assay, and ELISA tyrosine phosphatase IA-2 antibody (IA-2A) and insulin antibody (IA) assays.Research Design and Methods. Antibodies were tested in 438 children and adolescents with newly diagnosed diabetes mellitus (DM) type 1, and in 891 subjects without DM type 1. ICA were determined by the classic indirect immunofluorescent method recommended by the Juvenile Diabetes Foundation International, GADA were determined with the Immunotech IRMA Anti-GAD kit, and IA-2A and IA were determined with Medizym Anti-IA2 and Orgentec Anti-Insulin ELISA kits, respectively. Sensitivity (Se), specificity (Sp), positive predictive value (PPV) and negative predictive value (NPV) of the tests were estimated with contingency tables. Diagnostic accuracy was estimated from areas under receiver operating curves (AUC).Results. ICA test was of the greatest diagnostic value (Se=88%, Sp=96%, PPV=96%, NPV=94%, AUC=0,94), followed by IA-2A (Se=66%, Sp=98%, PPV=98%, NPV=59%, AUC=0,82) and GADA (Se=73%, Sp=84%, PPV=75%, NPV=83%, AUC=0,79). IA test exhibited a very low Se (4,3%) and lacked diagnostic accuracy (AUC=0,5).Conclusions. We recommend to use ICA, IA-2A and GADA tests surveyed in our study for diagnosis of DM type 1 and differential diagnosis of DM. We don’t recommend IA testing with an Orgentec Anti-Insulin ELISA kit for usage in clinical practice. 

scholarly journals Diagnostic value of islet autoantibody assays practised in Russia. 1. Classic immunofluorescence islet cell antibody assay, immunoradiometric glutamic acid decarboxylase antibody assay, and ELISA tyrosine phosphatase antibody and insulin antibody assays.

10.14341/8032 ◽  
2016 ◽  
Vol 19 (4) ◽  
pp. 331
Author(s):  
Alexei V. Timofeev ◽  
Ksenia A. Gorst ◽  
Valentin Y. Uvarov ◽  
Ekaterina A. Pronina ◽  
Alisa V. Vitebskaya ◽  
...  
Keyword(s):  
Diagnostic Accuracy ◽  
Islet Cell ◽  
Tyrosine Phosphatase ◽  
Diagnostic Value ◽  
Islet Cell Antibody ◽  
Acid Decarboxylase ◽  
Antibody Assay ◽  
Glutamic Acid Decarboxylase Antibody ◽  
Cell Antibody

Objective. To estimate performance characteristics and diagnostic value of immunofluorescent islet cell antibody (ICA) assay, immunoradiometric glutamic acid decarboxylase antibody (GADA) assay, and ELISA tyrosine phosphatase IA-2 antibody (IA-2A) and insulin antibody (IA) assays.Research Design and Methods. Antibodies were tested in 438 children and adolescents with newly diagnosed diabetes mellitus (DM) type 1, and in 891 subjects without DM type 1. ICA were determined by the classic indirect immunofluorescent method recommended by the Juvenile Diabetes Foundation International, GADA were determined with the Immunotech IRMA Anti-GAD kit, and IA-2A and IA were determined with Medizym Anti-IA2 and Orgentec Anti-Insulin ELISA kits, respectively. Sensitivity (Se), specificity (Sp), positive predictive value (PPV) and negative predictive value (NPV) of the tests were estimated with contingency tables. Diagnostic accuracy was estimated from areas under receiver operating curves (AUC).Results. ICA test was of the greatest diagnostic value (Se=88%, Sp=96%, PPV=96%, NPV=94%, AUC=0,94), followed by IA-2A (Se=66%, Sp=98%, PPV=98%, NPV=59%, AUC=0,82) and GADA (Se=73%, Sp=84%, PPV=75%, NPV=83%, AUC=0,79). IA test exhibited a very low Se (4,3%) and lacked diagnostic accuracy (AUC=0,5).Conclusions. We recommend to use ICA, IA-2A and GADA tests surveyed in our study for diagnosis of DM type 1 and differential diagnosis of DM. We don’t recommend IA testing with an Orgentec Anti-Insulin ELISA kit for usage in clinical practice. 

The Combination of Antibodies to GAD-65 and IA-2ic Can Replace the Islet-Cell Antibody Assay to Identify Subjects at Risk of Type 1 Diabetes Mellitus

1999 ◽  
Vol 31 (10) ◽  
pp. 564-569 ◽  
Author(s):  
E. Hatziagelaki ◽  
C. Jaeger ◽  
R. Petzoldt ◽  
J. Seissler ◽  
W. Scherbaum ◽  
...  
Keyword(s):  
Diabetes Mellitus ◽  
At Risk ◽  
Type 1 Diabetes ◽  
Type 1 Diabetes Mellitus ◽  
Islet Cell ◽  
Islet Cell Antibody ◽  
Antibody Assay ◽  
Cell Antibody ◽  
Gad 65

scholarly journals Factors associated with increased irisin levels in the type 1 diabetes mellitus

2017 ◽  
Vol 51 (1) ◽  
pp. 1-7 ◽  
Author(s):  
I. Ates ◽  
M. F. Arikan ◽  
K. Erdogan ◽  
M. Kaplan ◽  
M. Yuksel ◽  
...  
Keyword(s):  
Diabetes Mellitus ◽  
Type 1 Diabetes ◽  
Type 1 Diabetes Mellitus ◽  
Glycosylated Hemoglobin ◽  
Islet Cell Antibody ◽  
Acid Decarboxylase ◽  
Insulin Autoantibody ◽  
Cell Antibody ◽  
Positive Groups

Abstract Objective. The aim of the present study was to determine the irisin levels in patients with the type 1 diabetes mellitus (T1DM) and to examine the relation of irisin levels with the inflammation and autoimmunity.Methods. This study included 35 cases diagnosed with T1DM and 36 healthy volunteers. Antiglutamic acid decarboxylase (anti-GAD), islet cell antibody (ICA), and insulin autoantibody levels were measured in patients at the time when they were included into the study and recorded from the patient files. Serum irisin levels were measured by ELISA kit.Results. The median irisin levels were determined higher in T1DM group compared to the control one (6.8 ng/ml vs. 4.8 ng/ml, p=0.022; respectively). Median irisin levels were higher in anti-GAD (p=0.022) and ICA (p=0.044) positive groups compared to negative groups. In T1DM group, irisin levels displayed positive correlation with glycosylated hemoglobin (HbA1c) (r=0.377, p<0.001) and anti-GAD (r=0.392, p=0.020) and negative correlation with creatinine (r=-0390, p=0.021). In multivariate regression model, HbA1c (B±SE: 2.76±17683, p<0.001), and anti-GAD (B±SE: 2.311±0.610, p=0.001) were determined as independent predictors for predicting the irisin levels.Conclusion. In patients with T1DM, which chronic inflammation and autoimmunity take part in their etiopathogenesis, anti-GAD levels were an independent risk factor for the irisin. Th is may suggest that factors such as inflammation and autoimmunity can be effective in the synthesis of irisin.

Predictive value of islet cell antibody titer at onset of type 1 diabetes for clinical course of the disease

2000 ◽  
Vol 50 ◽  
pp. 347
Author(s):  
A Jotic ◽  
N.M Lalic ◽  
M Zamaklar ◽  
M Bukelica ◽  
K Lalic ◽  
...  
Keyword(s):  
Type 1 Diabetes ◽  
Antibody Titer ◽  
Predictive Value ◽  
Islet Cell ◽  
Clinical Course ◽  
Islet Cell Antibody ◽  
Cell Antibody
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