scholarly journals The influence of growth regulators and explant position on the growth and development of Mandevilla sanderi (Hemsl.) Woodson in vitro

2021 ◽  
Vol 20 (5) ◽  
pp. 127-138
Author(s):  
Danuta Kozak ◽  
Marzena Parzymies ◽  
Alicja Świstowska ◽  
Barbara Marcinek ◽  
Elżbieta Pogroszewska
Keyword(s):  
Growth Regulators ◽  
Growth And Development ◽  
Callus Formation ◽  
Propagation Rate ◽  
Multiplication Rate ◽  
Explant Position ◽  
Murashige And Skoog Medium ◽  
The Media ◽  
Pot Plant

Mandevilla is a valuable ornamental pot vine. However, due to a low propagation rate, it is difficult to keep up with the demand. Micropropagation would allow to produce lots of plants for the market. The aim of the study was to determine the effect of the growth regulators addition to the media and explants orientation on multiplication of Mandevilla sanderi, an exotic, ornamental pot plant. The shoot tips were placed vertically or horizontally on the Murashige and Skoog medium supplemented with benzyladenine (BA) or isopentenyladenine (2iP), at concentrations of 1, 2.5 or 5 mg·dm–3 singly or in combination with thidiazuron (TDZ) at concentrations of 0.01, 0.025 or 0.05 mg·dm–3. Maximum multiplication rate was noted on the media supplemented with 2.5 mg·dm–3 2iP + 0.025 mg·dm–3 TDZ or 5 mg·dm–3 2iP, when explants were placed horizontally. All the treatments resulted in callus formation. Medium supplemented with the highest concentration of BA combined with TDZ was the most active in callus growth.

scholarly journals In vitro meristem-tip culture and regeneration approaches in Congolese cassava accessions (Manihot esculenta Crantz cv. Boma and cv. Mpelo-Nlongi)

2015 ◽  
Vol 3 (2) ◽  
pp. 72
Author(s):  
J.-Roger Bansimba Mukiese ◽  
Aimé Diamuini Ndofunsu ◽  
Freddy Bulubulu ◽  
Alexandre Mbaya Ntumbula ◽  
Sébastien Luyindula Ndiku
Keyword(s):  
Growth Regulators ◽  
Manihot Esculenta ◽  
Callus Formation ◽  
Shoot Development ◽  
Media Composition ◽  
Manihot Esculenta Crantz ◽  
Adenine Sulphate ◽  
Meristem Tip Culture ◽  
The Media

<p>Shiny dome-like structures measuring less than 1mm in length were excised aseptically from shoot tip buds of infected of two cassava (<em>Manihot esculenta</em> Crantz) local cultivars (Boma and Mpelo Nlongi) and cultivated <em>in vitro</em> in two types of media with different combination of growth hormone: Murashige and Skoog supplemented of sucrose (20 g/l), Naphtalenacetic acid (NAA, 10 μM), Ben-zylaminopurine (BAP, 0.66 μM) as well as Gibberellic acid (GA3, 0.1 μM) with 80 mg/l of Adenine sulphate and MS-free growth regulators. After four weeks, data were scored: 29.5% responding explant with callus formation and 20.5% responding explants to shoot development in the medium with growth regulators for the cultivar Boma whereas the cultivar Mpelo-Nlongi presented 5.7% and 25.7% respectively of callus formation and shoot development. The cultivar Boma presented a tendency more pronounced for the callus formation rather than with the shoot development contrary to the cultivar Mpelo-Nlongi. In regards of this experiment, it was shown that the media composition and genotype are essential factors, which influence in vitro growth, mainly the shoot development, in the culture of meristems for cassava local accessions.</p>

scholarly journals In vitro propagation from nodal segments of Lippia origanoides (chemotype A)

2022 ◽  
Vol 52 (7) ◽  
Author(s):  
Julián Sebastián Ramírez Moreno ◽  
Sergio Andrés Vega Porras ◽  
Roosevelt Humberto Escobar ◽  
Elena E. Stashenko ◽  
Jorge Luis Fuentes Lorenzo ◽  
...  
Keyword(s):  
Plant Growth ◽  
Gibberellic Acid ◽  
Growth Regulators ◽  
In Vitro Propagation ◽  
Callus Formation ◽  
Nodal Segments ◽  
Ms Medium ◽  
Shoot Number ◽  
Murashige And Skoog Medium

ABSTRACT: This research described an efficient micropropagation protocol for Lippia origanoides (Verbenaceae). Sterile seeds were used to obtain germinated seedlings in Murashige and Skoog medium (MS) supplemented with sucrose and agar. The nodal segments obtained from seedlings were grown on MS medium supplemented with different concentrations of gibberellic acid (GA), benzylaminopurine (BAP) and 1-naphthalenacetic acid (NAA) with BAP. The callus induction, shoots length, shoots number and root length, were analyzed. The treatments showed high percentage of callus formation at 0.5 to 1.5 mg L-1 of BAP alone or in combination with NAA (0.1 mg L-1). The highest value of shoot number per nodal segments was obtained at 1.5 mg L-1 of BAP (4.3 ± 0.8). The obtained plantlets were better rooted in vitro in the absence of plant growth regulators (PGRs) and they showed acclimatization rate of 90%. We reported a protocol for in vitro propagation and acclimatization of L. origanoides for A chemotypes from Colombia.

scholarly journals The influence of explants type and orientation on growth and development of Mandevilla sanderi (Hemsl.) Woodson in vitro

2019 ◽  
Vol 18 (4) ◽  
pp. 111-119
Author(s):  
Danuta Kozak ◽  
Marzena Parzymies ◽  
Alicja Świstowska ◽  
Barbara Marcinek ◽  
Bairam Solomon Ismael
Keyword(s):  
Shoot Tips ◽  
Multiplication Rate ◽  
Normal Position ◽  
Leafy Shoot ◽  
Different Types ◽  
The Media ◽  
Short Time ◽  
Vertical Positioning ◽  
Pot Plant

Mandevilla sanderi is an important commercial ornamental pot plant. Traditional vegetative propagation is limited due to the low rate, therefore there is a need to develop an alternative, more efficient method. There is an interest in development of micropropagation technology for the species, as it allows to obtain a lot of offsprings in a relatively short time. The aim of the present work was to estimate an influence of explants type and position on regeneration of Mandevilla sanderi in tissue culture. Four different types of explants (leafy shoot tips, decapitated leafy shoot tips, defoliated shoot tips, decapitated and defoliated shoot tips) were used in the experiment, which were placed on the media vertically, while defoliated shoot tips were placed horizontally or vertically upside down. The explants were cultivated on a Murashige and Skoog (MS) medium supplemented with 1 mg·dm–3 benzyladenine (BA) and 0.5 mg·dm–3 indole-3-butyric acid (IBA). It was noted that both explants orientation and positioning, influenced the multiplication rate. Defoliated shoot tips placed horizontally were characterized by higher multiplication rate (6.8) in comparison to upside down vertical positioning (3.2). It was also observed that removal of shoot apex improved axillary branching, while defoliation of shoots placed in a normal position reduced multiplication rate.

scholarly journals Assessment of In-vitro culture through Nodal explants of Dendrocalamus hamiltonii

2021 ◽  
Vol 2 (1) ◽  
pp. 130-133
Author(s):  
Abha Jha ◽  
◽  
Sunila Das ◽  
Keyword(s):  
Experimental Study ◽  
Growth Regulators ◽  
In Vitro Regeneration ◽  
Shoot Multiplication ◽  
Growth Hormones ◽  
Nodal Explants ◽  
Bud Break ◽  
Multiplication Rate ◽  
Dendrocalamus Hamiltonii

The present experimental study was aimed to overcome the traditional methods of propagation that limit the number of propagules by in-vitro regeneration through nodal explants of Dendrocalamus hamiltonii with a comparative study of growth regulators during the shooting and rooting process. Dendrocalamus hamiltonii is distributed from the Himalayas (Nepal) to the northern part of Burma. Collection of explants was done from different selected sites of CPTs. There was the use of HgCl2 and Ca (OCl)2 as sterilizing agents in different concentrations and its effect was visualized during the sprouting stage. Culm explants were cultured in a bottle containing White media (Wm) supplemented with BA and Kinetin for sprouting and IAA, IBA, NAA for rooting. There is also the use of IAA+IBA+NAA in combined form as a supplementary solution 0.1% HgCl2 treatment for 20-minute results into77.80% aseptic buds and 72% bud -break. Among the used growth-hormones, BA with concentration 0.25mg/l and 0.50mg/l respectively were appropriate for shoot-multiplication rate, 4.01±0.3 and 4.3±0.4 were ideal observation incorporation with BA (1.00mg/l) and BA (1.50mg/l) respectively. Maximum sprouting rate14.77±3.37with application of BA (2.00mg/l) and maximum shoot length4.3±0.4 is observed at BA (1.50mg/l). The applications of rooting hormone IAA+IBA+NAA in the concentration of 1.0 mg/l results in 72.5±0.3(rooting) and 11.1±0.3 (av. No. of the root).

In vitro plant regeneration from leaf expiants of Lycopersicon esculentum (tomato)

1976 ◽  
Vol 54 (21) ◽  
pp. 2409-2414 ◽  
Author(s):  
R. M. Behki ◽  
S. M. Lesley
Keyword(s):  
Plant Regeneration ◽  
Lycopersicon Esculentum ◽  
Growth Regulators ◽  
In Vitro Plant Regeneration ◽  
Leaf Discs ◽  
Skoog Medium ◽  
Murashige And Skoog Medium ◽  
Napthaleneacetic Acid ◽  
In Vitro Plant

Leaf discs from 15 mutant clones of tomato were tested for their morphogenetic response in Murashige and Skoog medium supplemented with 12 combinations of the growth regulators napthaleneacetic acid (NAA) and benzylaminopurine (BA) and 4 combinations of NAA and zeatin. The results show that either callus, shoots, roots, or shoots and roots can be produced depending upon the hormone concentrations and ratios. Plants were regenerated from 12 of the 15 varieties tested.

Effects of explant position and orientation, medium ph and nitrogen sources on micropropagation of blackberry

2021 ◽  
Vol 12 (4) ◽  
pp. 84-92
Author(s):  
Thábata Cristina Faxina de Aguiar ◽  
Ana Paola Negri ◽  
Pedro Boff ◽  
Murilo Dalla Costa ◽  
Mari Inês Carissimi Boff ◽  
...  
Keyword(s):  
Nitrogen Source ◽  
Nitrogen Sources ◽  
Original Position ◽  
Multiplication Rate ◽  
Highest Weight ◽  
Explant Position ◽  
Rubus Species ◽  
Position And Orientation ◽  
Ph Level

Composition of the medium and the explant origin are factors that interfere on success of micropropagation of Rubus species. For blackberry cultivar LochNess it was not investigated yet how the position and orientation of explant, pH levels and nitrogen source interfere on micropropagation. In this work, focused on the establishment of in vitro culture, variables were studied on R. fruticosus cv Loch Ness, such as the choice of the explants depending on their original position on the mother plant, pH level and nitrogen sources of the culture medium. For the first time in vitro on Rubus, the downward orientation (capogatto) of shoot tips explants was compared with the normal upward orientation. The highest weight and length values were recorded for the shoots proliferated from basal and nodal explants. For the initiation medium, the best multiplication rate were obtained in pH adjusted to 4.5. Shoot length was influenced by the nitrogen source; when associated with an increased light intensity, the complete substitution of ammonium by nitrate allowed results comparable with those obtained with the control medium containing both sources. The use of aminoacids did not improve the results. Apex orientation did not affect anatomical parameters or rooting rates of wild Rubus, but more efforts should be devoted on in vitro capogatto technique considering that advantages like reduction of plant growth regulators, cultivation on the same medium culture for more time and easily rooting can be established.

scholarly journals In vitro growth and development of Dendrobium hybrid orchid

1970 ◽  
Vol 35 (3) ◽  
pp. 507-514 ◽  
Author(s):  
H Khatun ◽  
MM Khatun ◽  
MS Biswas ◽  
MR Kabir ◽  
M Al-Amin
Keyword(s):  
Growth Regulators ◽  
Growth And Development ◽  
Ms Medium ◽  
Fresh Weight ◽  
Maximum Weight ◽  
Shoot Height ◽  
Single Shoot ◽  
Number Of Leaves ◽  
Regenerated Plantlets

The experiment was conducted to investigate the combined effect of different plant growth regulators and charcoal supplementation in MS medium on growth and development of plantlets regenerated from protocorm like bodies (PLBs) of hybrid orchid. The combination of BAP + NAA, BAP + IAA, BAP + IBA, and IAA + IBA at different concentrations with charcoal supplementation was studied. The result revealed that the use of different growth regulators had significant effect on different parameters studied. The maximum weight of PLBs (5.123 g) was obtained from the combination of BAP + IBA at 1.0 mg/l each. The highest shoot height (3.239 cm) and maximum number of rooted plantlets (4.473) was obtained from 1.0 mg/l each of BAP + NAA combination. The maximum number of leaves (3.490) and the maximum length of leaves (1.946 cm) were obtained from 1.0 mg/l each of BAP + IBA and the highest leaf width (1.166 cm) was obtained from 0.5 mg/l BAP +1.0 mg/l IBA combination. The highest root length was obtained from 0.5 mg/l each of BAP + IAA and the maximum number of regenerated plantlets (20) was obtained from 0.5 mg/l IAA + 1.0 mg/l IBA combination. However, the maximum fresh weight of single shoot (0.219 g) and the maximum number of roots per plantlet (6.300) was obtained from 1.0 mg/I each of IAA + IBA combination. Keywords: Dedrobium; orchid; hybrid; In vitro growth. DOI: 10.3329/bjar.v35i3.6457Bangladesh J. Agril. Res. 35(3) : 507-514

Effect of Hypocotyl Length on Morphogenesis of Explants of Cucumber (Cucumis sativus L.) in vitro

1992 ◽  
Vol 19 (2) ◽  
pp. 165
Author(s):  
RL Gambley ◽  
W Dodd
Keyword(s):  
Cucumis Sativus ◽  
Growth Regulators ◽  
Multiple Shoots ◽  
Root Production ◽  
Concomitant Increase ◽  
Hypocotyl Length ◽  
Rapid Reduction ◽  
Cucumis Sativus L ◽  
Murashige And Skoog Medium

Explants of cucumber seedlings having different lengths of hypocotyl attached were grown axenically on Murashige and Skoog medium supplemented with kinetin (2 mg L-1). Multiple shoots developed from the apical regions of all explants. In this tissue shoots may also develop at the base of the hypocotyl, but this response is strongly dependent upon the length of the hypocotyl. As the length of the hypocotyl increased beyond 5 mm, there was a rapid reduction in basal shoot numbers and a concomitant increase in root production. We suggest that these responses are related not to the ratio or concentration of endogenous growth regulators but to different regions of sensitivity to growth regulators along the hypocotyl.

Isozyme assessment of the genetic stability of micropropagated hybrid larch (Larix × eurolepis Henry)

1995 ◽  
Vol 25 (7) ◽  
pp. 1103-1112 ◽  
Author(s):  
Sylvie Richard ◽  
Sylvie Gauthier ◽  
Sylvie Laliberté
Keyword(s):  
Tissue Culture ◽  
Growth Regulators ◽  
Genetic Stability ◽  
Physiological Response ◽  
Culture Conditions ◽  
Hybrid Larch ◽  
Short Shoot ◽  
In Vitro Shoots ◽  
The Media

The search for the occurrence of somaclonal variation of in vitro shoots and acclimatized plants of a hybrid larch (Larix × urolepis Henry) clone was performed by the analysis of eight isozyme systems. Cultures were established from short shoot buds of mature material. The effects of growth regulators in the media, subculture intervals, and periods in culture were analyzed for in vitro shoots. Variability was found in in vitro shoots but appeared to be related to a physiological response to culture conditions. Once acclimatized, most tissuecultured plants expressed the same enzymatic patterns as those of control plants (stecklings and the ortet). The variations observed for some acclimatized plants were also observed in control plants and were not related to ontogenic stage. Results from the isoenzymatic systems studied showed that hybrid larch plants regenerated from tissue culture were not significantly different from stecklings and the ortet.

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