scholarly journals Perilipin 4 in human skeletal muscle: localization and effect of physical activity

2015 ◽  
Vol 3 (8) ◽  
pp. e12481 ◽  
Author(s):  
Shirin Pourteymour ◽  
Sindre Lee ◽  
Torgrim M. Langleite ◽  
Kristin Eckardt ◽  
Marit Hjorth ◽  
...  
Keyword(s):  
Physical Activity ◽  
Skeletal Muscle ◽  
Human Skeletal Muscle

Roles of sedentary aging and lifelong physical activity in exchange of glutathione across exercising human skeletal muscle

2014 ◽  
Vol 73 ◽  
pp. 166-173 ◽  
Author(s):  
Michael Nyberg ◽  
Stefan P. Mortensen ◽  
Helena Cabo ◽  
Mari-Carmen Gomez-Cabrera ◽  
Jose Viña ◽  
...  
Keyword(s):  
Physical Activity ◽  
Skeletal Muscle ◽  
Human Skeletal Muscle

scholarly journals Lifelong Physical Activity Prevents Aging-Associated Insulin Resistance in Human Skeletal Muscle Myotubes via Increased Glucose Transporter Expression

PLoS ONE ◽  
2013 ◽  
Vol 8 (6) ◽  
pp. e66628 ◽  
Author(s):  
Tipwadee Bunprajun ◽  
Tora Ida Henriksen ◽  
Camilla Scheele ◽  
Bente Klarlund Pedersen ◽  
Charlotte Jane Green
Keyword(s):  
Physical Activity ◽  
Insulin Resistance ◽  
Skeletal Muscle ◽  
Human Skeletal Muscle ◽  
Glucose Transporter ◽  
Transporter Expression

Myostatin in relation to physical activity and dysglycaemia and its effect on energy metabolism in human skeletal muscle cells

2015 ◽  
Vol 217 (1) ◽  
pp. 45-60 ◽  
Author(s):  
M. Hjorth ◽  
S. Pourteymour ◽  
S. W. Görgens ◽  
T. M. Langleite ◽  
S. Lee ◽  
...  
Keyword(s):  
Physical Activity ◽  
Skeletal Muscle ◽  
Energy Metabolism ◽  
Human Skeletal Muscle ◽  
Muscle Cells ◽  
Skeletal Muscle Cells ◽  
Human Skeletal Muscle Cells

scholarly journals Physical activity changes the regulation of mitochondrial respiration in human skeletal muscle

2002 ◽  
Vol 543 (1) ◽  
pp. 191-200 ◽  
Author(s):  
J. Zoll ◽  
H. Sanchez ◽  
B. N'Guessan ◽  
F. Ribera ◽  
E. Lampert ◽  
...  
Keyword(s):  
Physical Activity ◽  
Skeletal Muscle ◽  
Mitochondrial Respiration ◽  
Human Skeletal Muscle

scholarly journals DNA methylation across the genome in aged human skeletal muscle tissue and stem cells: The role of HOX genes and physical activity

2019 ◽  
Author(s):  
DC Turner ◽  
PP Gorski ◽  
MF Maasar ◽  
RA Seaborne ◽  
P Baumert ◽  
...  
Keyword(s):  
Physical Activity ◽  
Skeletal Muscle ◽  
Dna Methylation ◽  
Stem Cells ◽  
Axon Guidance ◽  
Muscle Tissue ◽  
Focal Adhesion ◽  
Human Skeletal Muscle ◽  
Hox Genes ◽  
Muscle Stem Cells

AbstractSkeletal muscle tissue demonstrates global hypermethylation with aging. However, methylome changes across the time-course of differentiation in aged human muscle derived stem cells, and larger coverage arrays in aged muscle tissue have not been undertaken. Using 850K DNA methylation arrays we compared the methylomes of young (27 ± 4.4 years) and aged (83 ± 4 years) human skeletal muscle and that of young/aged muscle stem cells over several time points of differentiation (0, 72 hours, 7, 10 days). Aged muscle tissue was hypermethylated compared with young tissue, enriched for; ‘pathways-in-cancer’ (including; focal adhesion, MAPK signaling, PI3K-Akt-mTOR signaling, p53 signaling, Jak-STAT signaling, TGF-beta and notch signaling), ‘rap1-signaling’, ‘axon-guidance’ and ‘hippo-signalling’. Aged muscle stem cells also demonstrated a hypermethylated profile in pathways; ‘axon-guidance’, ‘adherens-junction’ and ‘calcium-signaling’, particularly at later timepoints of myotube formation, corresponding with reduced morphological differentiation and reductions in MyoD/Myogenin gene expression compared with young cells. While young cells showed little alteration in DNA methylation during differentiation, aged cells demonstrated extensive and significantly altered DNA methylation, particularly at 7 days of differentiation and most notably in the ‘focal adhesion’ and ‘PI3K-AKT signalling’ pathways. While the methylomes were vastly different between muscle tissue and isolated muscle stem cells, we identified a small number of CpG sites showing a hypermethylated state with age, in both muscle and tissue and stem cells (on genes KIF15, DYRK2, FHL2, MRPS33, ABCA17P). Most notably, differential methylation analysis of chromosomal regions identified three locations containing enrichment of 6-8 CpGs in the HOX family of genes altered with age. With HOXD10, HOXD9, HOXD8, HOXA3, HOXC9, HOXB1, HOXB3, HOXC-AS2 and HOXC10 all hypermethylated in aged tissue. In aged cells the same HOX genes (and additionally HOXC-AS3) displayed the most variable methylation at 7 days of differentiation versus young cells, with HOXD8, HOXC9, HOXB1 and HOXC-AS3 hypermethylated and HOXC10 and HOXC-AS2 hypomethylated. We also determined that there was an inverse relationship between DNA methylation and gene expression for HOXB1, HOXA3 and HOXC-AS3. Finally, increased physical activity in young adults was associated with oppositely regulating HOXB1 and HOXA3 methylation compared with age. Overall, we demonstrate that a considerable number of HOX genes are differentially epigenetically regulated in aged human skeletal muscle and muscle stem cells and increased physical activity may help prevent age-related epigenetic changes in these HOX genes.

Effects Of Old Age And Physical Activity On Oxidative Capacity In Human Skeletal Muscle

2009 ◽  
Vol 41 ◽  
pp. 131 ◽  
Author(s):  
Ryan G. Larsen ◽  
Damien M. Callahan ◽  
Stephen A. Foulis ◽  
Jane A. Kent-Braun
Keyword(s):  
Physical Activity ◽  
Skeletal Muscle ◽  
Old Age ◽  
Human Skeletal Muscle ◽  
Oxidative Capacity

Aging and Physical Activity: Effects on Human Skeletal Muscle Phenotype

2015 ◽  
Vol 39 (2) ◽  
pp. 170
Author(s):  
F. St-Jean Pelletier ◽  
C.H. Pion ◽  
F. Lemieux ◽  
J.P. Leduc-Gaudet ◽  
S. Barbat-Artigas ◽  
...  
Keyword(s):  
Physical Activity ◽  
Skeletal Muscle ◽  
Human Skeletal Muscle

scholarly journals DNA methylation across the genome in aged human skeletal muscle tissue and muscle-derived cells: the role of HOX genes and physical activity

2020 ◽  
Vol 10 (1) ◽  
Author(s):  
D. C. Turner ◽  
P. P. Gorski ◽  
M. F. Maasar ◽  
R. A. Seaborne ◽  
P. Baumert ◽  
...  
Keyword(s):  
Gene Expression ◽  
Physical Activity ◽  
Skeletal Muscle ◽  
Dna Methylation ◽  
Axon Guidance ◽  
Muscle Tissue ◽  
Focal Adhesion ◽  
Human Skeletal Muscle ◽  
Hox Genes ◽  
Skeletal Muscle Tissue

Abstract Skeletal muscle tissue demonstrates global hypermethylation with age. However, methylome changes across the time-course of differentiation in aged human muscle derived cells, and larger coverage arrays in aged muscle tissue have not been undertaken. Using 850K DNA methylation arrays we compared the methylomes of young (27 ± 4.4 years) and aged (83 ± 4 years) human skeletal muscle and that of young/aged heterogenous muscle-derived human primary cells (HDMCs) over several time points of differentiation (0, 72 h, 7, 10 days). Aged muscle tissue was hypermethylated compared with young tissue, enriched for; pathways-in-cancer (including; focal adhesion, MAPK signaling, PI3K-Akt-mTOR signaling, p53 signaling, Jak-STAT signaling, TGF-beta and notch signaling), rap1-signaling, axon-guidance and hippo-signalling. Aged cells also demonstrated a hypermethylated profile in pathways; axon-guidance, adherens-junction and calcium-signaling, particularly at later timepoints of myotube formation, corresponding with reduced morphological differentiation and reductions in MyoD/Myogenin gene expression compared with young cells. While young cells showed little alterations in DNA methylation during differentiation, aged cells demonstrated extensive and significantly altered DNA methylation, particularly at 7 days of differentiation and most notably in focal adhesion and PI3K-AKT signalling pathways. While the methylomes were vastly different between muscle tissue and HDMCs, we identified a small number of CpG sites showing a hypermethylated state with age, in both muscle tissue and cells on genes KIF15, DYRK2, FHL2, MRPS33, ABCA17P. Most notably, differential methylation analysis of chromosomal regions identified three locations containing enrichment of 6–8 CpGs in the HOX family of genes altered with age. With HOXD10, HOXD9, HOXD8, HOXA3, HOXC9, HOXB1, HOXB3, HOXC-AS2 and HOXC10 all hypermethylated in aged tissue. In aged cells the same HOX genes (and additionally HOXC-AS3) displayed the most variable methylation at 7 days of differentiation versus young cells, with HOXD8, HOXC9, HOXB1 and HOXC-AS3 hypermethylated and HOXC10 and HOXC-AS2 hypomethylated. We also determined that there was an inverse relationship between DNA methylation and gene expression for HOXB1, HOXA3 and HOXC-AS3. Finally, increased physical activity in young adults was associated with oppositely regulating HOXB1 and HOXA3 methylation compared with age. Overall, we demonstrate that a considerable number of HOX genes are differentially epigenetically regulated in aged human skeletal muscle and HDMCs and increased physical activity may help prevent age-related epigenetic changes in these HOX genes.

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