Changes of different forms of active and inactive renin under stress in rats

In a new method for measurement of inactive rat plasma renin, the trypsin generated angiotensin I immunoreactive material, which was HPLC characterized as similar to tetradecapeptide renin substrate, is removed by a cation exchange resin before the renin incubation step. The method also corrects for trypsin destruction of endogenous angiotensinogen by the addition of exogenous angiotensinogen. When measured with this method inactive renin in rat plasma decreased after nephrectomy and increased after adrenalectomy. This is in accordance with findings in humans. A sexual dimorphism of prorenin (inactive renin) in rat plasma, similar to that reported in humans and mice, was demonstrated. Thus, inactive renin in the rat is no exception among species, and the rat might be a suitable animal model for further studies dealing with the physiology of prorenin in plasma and tissues.Key words: angiotensinogen, inactive renin, renin.

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Ontogeny of neuronally released norepinephrine on renin secretion in sheep

AJP Regulatory Integrative and Comparative Physiology ◽

10.1152/ajpregu.1989.257.4.r765 ◽

1989 ◽

Vol 257 (4) ◽

pp. R765-R770 ◽

Cited By ~ 1

Author(s):

K. T. Nakamura ◽

J. M. Klinkefus ◽

F. G. Smith ◽

T. Sato ◽

J. E. Robillard

Keyword(s):

Renin Secretion ◽

Renal Nerves ◽

Nerve Terminals ◽

Norepinephrine Release ◽

Fetal Sheep ◽

Postnatal Maturation ◽

Active Renin ◽

Inactive Renin ◽

Cortical Slices

The role of renal nerves and norepinephrine release on renin secretion during fetal and postnatal maturation has not been studied. Experiments were performed to determine the effect of veratridine, a substance known to promote norepinephrine release from nerve terminals, on active and inactive renin secretion from renal cortical slices of fetal (134-138 days gestation; term is 145 days), newborn (4-9 days of age), and adult nonpregnant sheep. Veratridine (10-300 microM) significantly increased active renin secretion and produced a small but nonsignificant rise in inactive renin secretion in all three groups of animals (P less than 0.05). The percent rise in active renin secretion during veratridine stimulation was similar among all groups. Veratridine-stimulated (300 microM) active renin secretion was antagonized by tetrodotoxin (0.5 and 5.0 microM) and DL-propranolol (1 microM) in fetal renal cortical slices. However, neither tetrodotoxin nor propranolol completely inhibited the stimulatory effect of veratridine on active renin secretion. These results suggest that 1) norepinephrine released from nerve terminals may regulate active renin secretion early during development; 2) the effect of veratridine on active renin secretion was similar in fetal, newborn, and adult sheep; 3) veratridine had no significant effect on inactive renin secretion; and 4) active renin secretion due to depolarization of nerve terminals in fetal sheep is dependent on activation of beta-adrenoceptors as it is in adults.

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Molecular Characterization of Inactive Renin: Complete Purification of Prorenin in Hog Kidney and Isolation of Inactive Renin from Neuroblastoma Cells: Evidence for 2 Different Types of Inactive Renin

Clinical and Experimental Hypertension Part A Theory and Practice ◽

10.3109/10641968209062364 ◽

1982 ◽

Vol 4 (11-12) ◽

pp. 1987-1994

Author(s):

T. Inagami ◽

Y. Takii ◽

T. Inagaki ◽

D. L. Clemens ◽

J. J. Chang ◽

...

Keyword(s):

Molecular Characterization ◽

Neuroblastoma Cells ◽

Different Types ◽

Complete Purification ◽

Inactive Renin ◽

Hog Kidney

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Plasma Inactive Renin in Patients with Hyperthyroidism

The Journal of Clinical Endocrinology & Metabolism ◽

10.1210/jcem-56-1-198 ◽

1983 ◽

Vol 56 (1) ◽

pp. 198-201 ◽

Cited By ~ 4

Author(s):

MITSUAKI NAKAMARU ◽

TOSHIO OGIHARA ◽

JITSUO HIGAKI ◽

YUICHI KUMAHARA ◽

KAZUO MURAKAMI ◽

...

Keyword(s):

Inactive Renin

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Does heparin inhibit renin activity?

Canadian Journal of Physiology and Pharmacology ◽

10.1139/y91-201 ◽

1991 ◽

Vol 69 (9) ◽

pp. 1360-1363 ◽

Cited By ~ 1

Author(s):

Masato Matsunaga ◽

Yoko Yamanaka ◽

Noriko Nagano ◽

Yuki Iwasaki ◽

Yumi Saito ◽

...

Keyword(s):

Smooth Muscle ◽

Vascular Smooth Muscle ◽

Smooth Muscle Cell ◽

Muscle Cell ◽

Vascular Smooth Muscle Cell ◽

Plasma Renin ◽

Renin Activity ◽

Renin Substrate ◽

Significant Difference ◽

Inactive Renin

Although heparin was reported in the 1960s to inhibit renin activity, this has not always been confirmed by other investigators. Hence, we re-examined whether heparin really inhibits renin or not. Renin activities were determined by radioimmunoassay of angiotensin I generated at pH 7.4. (i) No significant difference was found between the two kinds of plasma samples obtained with heparin and with EDTA as anticoagulant, in ARC (renin activity with addition of sheep renin substrate), TRC (ARC after activation of inactive renin by trypsin), or PRA (plasma renin activity without additional substrate), (ii) Even in higher concentrations of heparin up to 500 U/mL, neither PRA, ARC, nor TRC of plasma was affected significantly. (iii) Heparin, in concentrations up to 500 U/mL, exerted no significant effect on TRC of the media of human vascular smooth muscle cell culture. In conclusion, heparin does not exert any significant inhibitory effect on human renin nor does it affect activation of inactive renin by trypsin in the range of concentration of practical use, under the conditions employed in this study.Key words: plasma renin, tissue renin, inactive renin, vascular smooth muscle cell, trypsin.

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