Molecular Characterization of Inactive Renin: Complete Purification of Prorenin in Hog Kidney and Isolation of Inactive Renin from Neuroblastoma Cells: Evidence for 2 Different Types of Inactive Renin

Abstract Prolyl endopeptidase from an aerobic and Gram-negative thermophile Meiothermus ruber H328 (MrPEP) was purified in native and recombinant forms, but both preparations had comparable characteristics. Production of the native MrPEP was increased 10-fold by adding intact chicken feathers. The gene for MrPEP (mrH_2860) was cloned from the genome of strain H328 and found to have no signal sequence at the N-terminus. MrPEP is composed of two major domains: the β-propeller domain and the peptidase domain with a typical active site motif and catalytic triad. Based on extensive investigations with different types of peptide substrates and FRETS-25Xaa libraries, MrPEP showed strict preferences for Pro residue at the P1 position but broader preferences at the P2 and P3 positions in substrate specificity with stronger affinity for residues at the P3 position of substrate peptides that are longer than four residues in length. In conclusion, the molecular characterization of MrPEP resembles its animal counterparts more closely than bacterial counterparts in function and structure.

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Prevalence, virulence, antimicrobial resistance, and molecular characterization of fluoroquinolone resistance of Vibrio parahaemolyticus from different types of food samples in China

International Journal of Food Microbiology ◽

10.1016/j.ijfoodmicro.2019.108461 ◽

2020 ◽

Vol 317 ◽

pp. 108461 ◽

Cited By ~ 1

Author(s):

Tao Lei ◽

Fufeng Jiang ◽

Min He ◽

Jumei Zhang ◽

Haiyan Zeng ◽

...

Keyword(s):

Antimicrobial Resistance ◽

Molecular Characterization ◽

Vibrio Parahaemolyticus ◽

Food Samples ◽

Fluoroquinolone Resistance ◽

Different Types

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Molecular characterization of nine different types of mutants among 107 inhibitor-resistant TEM beta-lactamases from clinical isolates of Escherichia coli

Antimicrobial Agents and Chemotherapy ◽

10.1128/aac.39.2.427 ◽

1995 ◽

Vol 39 (2) ◽

pp. 427-430 ◽

Cited By ~ 76

Author(s):

C. Henquell ◽

C. Chanal ◽

D. Sirot ◽

R. Labia ◽

J. Sirot

Keyword(s):

Escherichia Coli ◽

Molecular Characterization ◽

Clinical Isolates ◽

Beta Lactamases ◽

Different Types

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Electron microscopic characterization of diamond thin films and substrates for diamond heteroepitaxial growth

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100154937 ◽

1989 ◽

Vol 47 ◽

pp. 592-593

Author(s):

J.B. Posthill ◽

R.P. Burns ◽

R.A. Rudder ◽

Y.H. Lee ◽

R.J. Markunas ◽

...

Keyword(s):

Thin Films ◽

Wide Band ◽

Deposition Process ◽

Heteroepitaxial Growth ◽

Electron Microscopic ◽

Wide Band Gap ◽

Lattice Matching ◽

Different Types ◽

Diamond Thin Films

Because of diamond’s wide band gap, high thermal conductivity, high breakdown voltage and high radiation resistance, there is a growing interest in developing diamond-based devices for several new and demanding electronic applications. In developing this technology, there are several new challenges to be overcome. Much of our effort has been directed at developing a diamond deposition process that will permit controlled, epitaxial growth. Also, because of cost and size considerations, it is mandatory that a non-native substrate be developed for heteroepitaxial nucleation and growth of diamond thin films. To this end, we are currently investigating the use of Ni single crystals on which different types of epitaxial metals are grown by molecular beam epitaxy (MBE) for lattice matching to diamond as well as surface chemistry modification. This contribution reports briefly on our microscopic observations that are integral to these endeavors.

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