scholarly journals Pre-analytical considerations in biomarker research: focus on cardiovascular disease

2021 ◽  
Vol 0 (0) ◽  
Author(s):  
Elena Revuelta-López ◽  
Jaume Barallat ◽  
Adriana Cserkóová ◽  
Carolina Gálvez-Montón ◽  
Allan S. Jaffe ◽  
...  
Keyword(s):  
Molecular Signature ◽  
Systematic Bias ◽  
Sample Collection ◽  
Valid Data ◽  
Fast Pace ◽  
Clinical Biomarker ◽  
Processing And Storage ◽  
Biomarker Research ◽  
And Storage ◽  
Analytical Errors

Abstract Clinical biomarker research is growing at a fast pace, particularly in the cardiovascular field, due to the demanding requirement to provide personalized precision medicine. The lack of a distinct molecular signature for each cardiovascular derangement results in a one-size-fits-all diagnostic and therapeutic approach, which may partially explain suboptimal outcomes in heterogeneous cardiovascular diseases (e.g., heart failure with preserved ejection fraction). A multidimensional approach using different biomarkers is quickly evolving, but it is necessary to consider pre-analytical variables, those to which a biological sample is subject before being analyzed, namely sample collection, handling, processing, and storage. Pre-analytical errors can induce systematic bias and imprecision, which may compromise research results, and are easy to avoid with an adequate study design. Academic clinicians and investigators must be aware of the basic considerations for biospecimen management and essential pre-analytical recommendations as lynchpin for biological material to provide efficient and valid data.

074 Controlled analysis of preanalytical variables in clinical blood and CSF sample collection, processing and storage: Implications for clinical research

Cryobiology ◽  
2013 ◽  
Vol 67 (3) ◽  
pp. 418-419
Author(s):  
Sushmita Mimi Roy ◽  
Geuncheol Gil ◽  
Bich Nguyen ◽  
Daniel Lopez-Ferrer ◽  
Xiaolei Xie ◽  
...  
Keyword(s):  
Clinical Research ◽  
Sample Collection ◽  
Processing And Storage ◽  
And Storage

scholarly journals Pre-analytical errors and patient safety / Preanalitičke greške i bezbednost pacijenata

2012 ◽  
Vol 31 (4) ◽  
pp. 265-270 ◽  
Author(s):  
Mario Plebani
Keyword(s):  
Information Technologies ◽  
Clinical Laboratory ◽  
Healthcare Personnel ◽  
Laboratory Diagnostics ◽  
Specimen Preparation ◽  
Sample Collection ◽  
Patient Preparation ◽  
Analytical Phase ◽  
And Storage ◽  
Analytical Errors

Summary Laboratory medicine, as a specialty that had prioritised quality control, has always been at the forefront of error reduction. In the last decades, a dramatic decrease of analytical errors has been experienced, while a relatively high frequency of errors has been documented in the pre-analytical phase. Most pre-analytical errors, which account for up to 70% of all mistakes made in laboratory diagnostics, arise during patient preparation, and sample collection, transportation, preparation for analysis and storage. However, while it has been reported that the pre-analytical phase is error-prone, only recently has it been demonstrated that most of these errors occur in the »pre-pre-analytical phase«, which comprises the initial procedures of the testing process performed outside the laboratory walls by healthcare personnel outside the direct control of the clinical laboratory. Developments in automation and information technologies have played a major role in decreasing some pre-analytical errors and, in particular, the automation of repetitive, errorprone and bio-hazardous pre-analytical processes performed within the laboratory walls has effectively decreased errors in specimen preparation, centrifugation, aliquot preparation, pipetting and sorting. However, more efforts should be made to improve the appropriateness of test request, patient and sample identification procedures and other pre-analytical steps performed outside the laboratory walls.

scholarly journals How Robust is the Evidence for a Role of Oxidative Stress in Autism Spectrum Disorders and Intellectual Disabilities?

2020 ◽  
Author(s):  
Shanna L. Burke ◽  
Jessica Cobb ◽  
Rumi Agarwal ◽  
Marlaina Maddux ◽  
Marcus S. Cooke
Keyword(s):  
Oxidative Stress ◽  
Autism Spectrum Disorders ◽  
Autism Spectrum ◽  
Careful Consideration ◽  
Sample Collection ◽  
Spectrum Disorders ◽  
Processing And Storage ◽  
And Storage ◽  
Biomarkers Of Oxidative Stress

Abstract Growing interest in the pathogenesis of autism spectrum disorders (ASDs) and other intellectual and developmental disabilities (IDD) has led to emerging evidence implicating a role for oxidative stress. However, understanding the strength of this association is made challenging by the use of a variety of purported biomarkers of oxidative stress, many of which have either uncertain specificity or flawed methods of analysis. This review aims to address this issue, which is widespread in the ASD and IDD literature, by providing readers with information concerning the strengths and limitations of the choice and analysis of biomarkers of oxidative stress. We highlight that biomarkers and assays should be specific, sensitive, reproducible, precise, robust, and chosen with careful consideration. Future studies should be sufficiently powered and address sample collection, processing, and storage which are, additionally, poorly considered, sources of bad practice, and potential errors. Only with these issues considered, will the data lead to conclusions as to the precise role of oxidative stress in ASDs and IDD.

Study Protocol Document forPONE-D-21-18427R2 (Prevalence and Predictors of GMD among PLWH on cART) v1

2021 ◽  
Author(s):  
Wondmagegn T. Tadesse ◽  
not provided Wondwossen Amonge ◽  
Eleni Akllilu ◽  
not provided Ephrem Engidawork
Keyword(s):  
Data Extraction ◽  
Sample Size Calculation ◽  
Study Participant ◽  
Glucose Metabolism Disorders ◽  
Sample Collection ◽  
Medical Record Data ◽  
Participant Recruitment ◽  
Record Data ◽  
Processing And Storage ◽  
And Storage

This is a document that states about sample size calculation, study participant recruitment, interview and medical record data extraction sheet, sample collection, processing and storage until lab analysis. Moreover, it states about how Glucose Metabolism Disorders (GMD) is determined with specific forms of GMDs.

scholarly journals Collection and Storage of Human Plasma for Measurement of Oxylipins

Metabolites ◽  
2021 ◽  
Vol 11 (3) ◽  
pp. 137
Author(s):  
Kristen J. Polinski ◽  
Michael Armstrong ◽  
Jonathan Manke ◽  
Jennifer Seifert ◽  
Tessa Crume ◽  
...  
Keyword(s):  
Human Plasma ◽  
Ethylenediaminetetraacetic Acid ◽  
Small Subset ◽  
Omega 3 ◽  
Sample Collection ◽  
Resolvin D1 ◽  
Heparin Plasma ◽  
Liquid Chromatography Tandem Mass ◽  
Processing And Storage ◽  
And Storage

Oxylipins derived from omega-3 and -6 fatty acids are actively involved in inflammatory and immune processes and play important roles in human disease. However, as the interest in oxylipins increases, questions remain regarding which molecules are detectable in plasma, the best methods of collecting samples, and if molecules are stable during collection and storage. We thereby built upon existing studies by examining the stability of an expanded panel of 90 oxylipins, including specialized pro-resolving lipid mediators (SPMs), in human plasma (n = 5 subjects) during sample collection, processing, and storage at −80 °C. Oxylipins were quantified using liquid chromatography-tandem mass spectrometry (LC/MS/MS). Blood samples collected in ethylenediaminetetraacetic acid (EDTA) or heparin followed by up to 2 h at room temperature prior to processing showed no significant differences in oxylipin concentrations compared to immediately processed samples, including the SPMs lipoxin A4 and resolvin D1. The majority of molecules, including SPMs, remained stable following storage for up to 1 year. However, in support of previous findings, changes were seen in a small subset of oxylipins including 12-HETE, TXB2, 14-HDHA, and 18-HEPE. Overall, this study showed that accurate measurements of most oxylipins can be obtained from stored EDTA or heparin plasma samples using LC/MS/MS.

TBS preanalytical phase working group survey study – preanalytical phase in coagulation laboratories

2020 ◽  
Vol 0 (0) ◽  
Author(s):  
Mehmet Senes ◽  
Berrin Bercik Inal ◽  
Fehime Benli Aksungar ◽  
Ipek Cinaroglu ◽  
Pinar Eker ◽  
...  
Keyword(s):  
Working Group ◽  
Survey Study ◽  
Sample Collection ◽  
Preanalytical Phase ◽  
Coagulation Tests ◽  
Patient Preparation ◽  
Survey Results ◽  
Processing And Storage ◽  
Almost All ◽  
And Storage

AbstractObjectivesPreanalytical phase quality, which begins with a request for testing and continues with patient preparation, sample collection, transport, processing and storage, is essential for coagulation tests. The Turkish Biochemical Society Preanalytical Phase Working Group created a survey to evaluate the preanalytical phase practices for coagulation laboratories in our country.MethodsThe survey consisted of a total of 26 questions and included almost all steps of the preanalytical phase.ResultsFifty-four laboratory specialists have participated in the study. The survey results showed that participants have different practices for most stages of the preanalytical phase for coagulation tests.ConclusionAccording to the survey results, a national guideline may help standardisation of the preanalytical phase for coagulation tests in our country and increasing training in this respect would contribute to achieving accurate test results.

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