Evaluation of the BD Vacutainer® PST™ II Blood Collection Tube for special chemistry analytes

2009 ◽  
Vol 47 (3) ◽  
Author(s):  
Jeffrey Chance ◽  
Julie Berube ◽  
Marita Vandersmissen ◽  
Norbert Blanckaert
Keyword(s):  
Luteinizing Hormone ◽  
Follicle Stimulating Hormone ◽  
Thyroid Stimulating Hormone ◽  
Initial Time ◽  
Blood Collection ◽  
Time Intervals ◽  
Collection Tube ◽  
Total Thyroxine ◽  
Blood Collection Tube ◽  
Stimulating Hormone

Abstract: The performance of the BD Vacutainer: Tubes were drawn by routine venipuncture from 42 subjects according to a randomized draw order. Tubes were processed and centrifuged according to recommended handling procedures. Serum and plasma from the comparison tubes were aliquoted to secondary containers prior to analysis. Specimens were then tested for selected special chemistry analytes at two time intervals (initial time and after 24 h storage). Analytes tested included thyroid stimulating hormone, free thyroxine, total thyroxine, follicle stimulating hormone, luteinizing hormone, ferritin, cortisol, vitamin B12, folate, and testosterone. The data were collected and analyzed by analysis of variance and mean bias comparisons.: The performance of the BD Vacutainer: The BD VacutainerClin Chem Lab Med 2009;47:358–61.

A Multicenter Evaluation of a Nongel Mechanical Separator Plasma Blood Collection Tube for Testing of Selected Therapeutic Drugs

2020 ◽  
Vol 5 (4) ◽  
pp. 671-685
Author(s):  
Svetlana Morosyuk ◽  
Julie Berube ◽  
Robert Christenson ◽  
Alan H B Wu ◽  
Denise Uettwiller-Geiger ◽  
...  
Keyword(s):  
The Other ◽  
Initial Time ◽  
Therapeutic Drug ◽  
Blood Collection ◽  
Test Results ◽  
Sample Storage ◽  
Therapeutic Drugs ◽  
Collection Tube ◽  
And Storage ◽  
Blood Collection Tube

Abstract Background Some therapeutic drugs are unstable during sample storage in gel tubes. BD Vacutainer® Barricor™ Plasma Blood Collection Tube with nongel separator was compared with plasma gel tubes, BD Vacutainer PST™, PST II, and BD Vacutainer Serum Tube for acetaminophen, salicylate, digoxin, carbamazepine, phenytoin, valproic acid, and vancomycin during sample storage for up to 7 days. Methods Seven hospital sites enrolled 705 participants who were taking at least one selected drug. The study tubes were collected and tested at initial time (0 h), after 48 h of storage at room temperature and on day 7 (after additional 5 days of refrigerated storage). The performance of BD Barricor tube was evaluated for each drug by comparing BD Barricor samples with samples from the other tubes at 0 h from the same participant; stability was evaluated by comparing test results from the same tube at 0 h, 48 h, and 7 days. Results At 0 h, BD Barricor showed clinically equivalent results for selected therapeutic drugs compared with the other tubes, except phenytoin in BD PST. Phenytoin samples ≥20 µg/mL in BD PST had 10–12% lower values than samples in BD Barricor. During sample storage, all selected drugs remained stable for 7 days in BD Barricor and in serum aliquots. In BD PST, all drugs remained stable except phenytoin and carbamazepine and in BD PST II except for phenytoin. Conclusion The BD Barricor Tube is effective for the collection and storage of plasma blood samples for therapeutic drug monitoring without sample aliquoting.

PRODUCTION OF ANTISERA AGAINST HIGHLY PURIFIED HUMAN FOLLICLE-STIMULATING HORMONE, LUTEINIZING HORMONE AND THYROID-STIMULATING HORMONE

1976 ◽  
Vol 70 (3) ◽  
pp. 335-344 ◽  
Author(s):  
J. I. THORELL ◽  
B. HOLMSTRÖM
Keyword(s):  
Luteinizing Hormone ◽  
Follicle Stimulating Hormone ◽  
Thyroid Stimulating Hormone ◽  
High Titre ◽  
Chorionic Gonadotrophin ◽  
Human Chorionic Gonadotrophin ◽  
Antigenic Determinants ◽  
High Avidity ◽  
Cross Reactions ◽  
Stimulating Hormone

SUMMARY Antisera were produced in rabbits against highly purified preparations of human LH (2000 or 10000 i.u./mg), human FSH (5500 i.u./mg), and human TSH (7·5 i.u./mg). Most rabbits produced antisera of high titre and high avidity. Cross-reactions were minimal between human TSH and human chorionic gonadotrophin (HCG) and between human FSH and HCG but marked between human LH and HCG. TSH and FSH also showed a constant but relatively weak cross-reaction. LH cross-reacted with FSH to a higher degree than did HCG. The avidity of the antisera was high. It was concluded that much of the lack of specificity recorded for glycoprotein antisera are effects of impure immunogens. Some of the true cross-reactions are probably explained by shared antigenic determinants of the β-subunits. Unadsorbed antisera could be used for assay of FSH and TSH in plasma from pregnant women.

DEXTRAN-COATED CHARCOAL USED IN THE RADIOIMMUNOASSAY OF HUMAN PITUITARY LUTEINIZING HORMONE

1973 ◽  
Vol 73 (3) ◽  
pp. 444-454 ◽  
Author(s):  
T. Sand ◽  
P. A. Torjesen
Keyword(s):  
Luteinizing Hormone ◽  
Follicle Stimulating Hormone ◽  
Protein Composition ◽  
Thyroid Stimulating Hormone ◽  
Human Chorionic Gonadotrophin ◽  
Human Thyroid ◽  
Serum Samples ◽  
Human Pituitary ◽  
The Difference ◽  
Stimulating Hormone

ABSTRACT A radioimmunoassay for human pituitary luteinizing hormone (LH) using charcoal for the separation of free from antibody-bound hormone is described. The ability of the various types of charcoal preparations tested to separate free from antibody-bound hormone differed greatly as did the amount required to give maximum adsorption of free hormone. It was also found that the adsorption of free and antibody-bound hormone was greatly influenced by the presence of other proteins. Hence it was necessary to add human serum to the standard tubes before the addition of the charcoal-dextran suspension, in order to compensate for the difference in protein composition between the standards and the serum samples. Two antisera obtained from rabbits immunized with human chorionic gonadotrophin (HCG) were used. One of the antisera had an affinity to human thyroid-stimulating hormone (TSH) and human follicle-stimulating hormone (FSH) of less than 10% as compared to that of LH, while the other had an affinity of about 30 % as compared to that of LH.

scholarly journals In-vitro hemolysis and its financial impact using different blood collection systems

2015 ◽  
Vol 0 (0) ◽  
Author(s):  
Janne Cadamuro ◽  
Georg Martin Fiedler ◽  
Cornelia Mrazek ◽  
Thomas Klaus Felder ◽  
Hannes Oberkofler ◽  
...  
Keyword(s):  
Financial Impact ◽  
Blood Collection ◽  
Laboratory Diagnostics ◽  
Time Interval ◽  
Time Intervals ◽  
Mean Values ◽  
The Past ◽  
Collection Tube ◽  
Blood Collection Tube

AbstractHemolytic specimens are among the most challenging preanalytical issues in laboratory diagnostics. The type of blood collection tube in use is claimed to influence in vitro hemolysis. We aimed to examine this hypothesis and estimate the respective financial impact, evaluating routine blood samples from the past 4 years.A total of 47,820 hemolysis index (HI) values from five different time intervals (IV1–IV5) were compared against each other, representing the following tubes: IV1-Sarstedt Monovette; IV2-8 mL/16×100 mm Greiner BioOne (GBO) Vacuette; IV3/IV4-5 mL/16×100 mm GBO Vacuette; IV5-4.5 mL/13×75 mm GBO Vacuette. For estimation of the economic impact, material, personnel and analytical costs were calculated.HI mean values in time interval IV2 were significantly higher than in all other intervals, while mean values amongst all other intervals were comparable. The number of moderately and severely hemolyzed samples increased with incrementing vacuum. Overall comparable costs between intervals IV1 and IV5 were €11,370, €14,045, €12,710, €11,213 and €8138 per 10,000 samples, respectively.Aspiration tubes and low vacuum tubes show comparable hemolysis rates. Increasing vacuum levels are associated with higher hemolysis rates. By decreasing in vitro hemolysis, financial savings up to €5907 per 10,000 samples could be gained.

A SOLID-PHASE RADIOIMMUNOASSAY FOR OVINE FOLLICLE-STIMULATING HORMONE

1973 ◽  
Vol 58 (2) ◽  
pp. 239-249 ◽  
Author(s):  
N. F. CUNNINGHAM ◽  
C. NANCY HEBERT
Keyword(s):  
Luteinizing Hormone ◽  
Blood Plasma ◽  
Solid Phase ◽  
Follicle Stimulating Hormone ◽  
Thyroid Stimulating Hormone ◽  
Cross Reaction ◽  
Sheep Blood ◽  
Solid Phase Radioimmunoassay ◽  
Stimulating Hormone

SUMMARY A radioimmunoassay using antibody-coated polystyrene tubes is described, which is suitable for the estimation of follicle-stimulating hormone (FSH) levels in sheep blood plasma. Ovine FSH was labelled with 125I by an enzymic method employing lactoperoxidase and H2O2, and was stable for several months at −15 °C. Antisera to ovine FSH were raised in rabbits. Standard curves for unlabelled FSH obtained by this method with different preparations of labelled FSH showed reproducible slopes from day to day. Ovine luteinizing hormone (LH) and thyroid-stimulating hormone (TSH) showed about 17% cross-reaction in the assay, necessitating the application of a correction to the FSH values for samples of plasma containing more than 15 ng of LH or TSH/ml. The FSH content of plasma from wethers and anoestrous ewes ranged from 20 to 120 ng/ml.

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