scholarly journals In-vitro hemolysis and its financial impact using different blood collection systems

2015 ◽  
Vol 0 (0) ◽  
Author(s):  
Janne Cadamuro ◽  
Georg Martin Fiedler ◽  
Cornelia Mrazek ◽  
Thomas Klaus Felder ◽  
Hannes Oberkofler ◽  
...  
Keyword(s):  
Financial Impact ◽  
Blood Collection ◽  
Laboratory Diagnostics ◽  
Time Interval ◽  
Time Intervals ◽  
Mean Values ◽  
The Past ◽  
Collection Tube ◽  
Blood Collection Tube

AbstractHemolytic specimens are among the most challenging preanalytical issues in laboratory diagnostics. The type of blood collection tube in use is claimed to influence in vitro hemolysis. We aimed to examine this hypothesis and estimate the respective financial impact, evaluating routine blood samples from the past 4 years.A total of 47,820 hemolysis index (HI) values from five different time intervals (IV1–IV5) were compared against each other, representing the following tubes: IV1-Sarstedt Monovette; IV2-8 mL/16×100 mm Greiner BioOne (GBO) Vacuette; IV3/IV4-5 mL/16×100 mm GBO Vacuette; IV5-4.5 mL/13×75 mm GBO Vacuette. For estimation of the economic impact, material, personnel and analytical costs were calculated.HI mean values in time interval IV2 were significantly higher than in all other intervals, while mean values amongst all other intervals were comparable. The number of moderately and severely hemolyzed samples increased with incrementing vacuum. Overall comparable costs between intervals IV1 and IV5 were €11,370, €14,045, €12,710, €11,213 and €8138 per 10,000 samples, respectively.Aspiration tubes and low vacuum tubes show comparable hemolysis rates. Increasing vacuum levels are associated with higher hemolysis rates. By decreasing in vitro hemolysis, financial savings up to €5907 per 10,000 samples could be gained.

The relationship between vacuum and hemolysis during catheter blood collection: a retrospective analysis of six large cohorts

2017 ◽  
Vol 55 (8) ◽  
pp. 1129-1134 ◽  
Author(s):  
Cornelia Mrazek ◽  
Ana-Maria Simundic ◽  
Helmut Wiedemann ◽  
Florian Krahmer ◽  
Thomas Klaus Felder ◽  
...  
Keyword(s):  
Real Life ◽  
Blood Collection ◽  
Aspiration System ◽  
Collection Tube ◽  
Tube Type ◽  
Evacuated Tubes ◽  
The Relationship ◽  
Blood Collection Tubes ◽  
Blood Collection Tube

Abstract Background: Blood collection through intravenous (IV) catheters is a common practice at emergency departments (EDs). This technique is associated with higher in vitro hemolysis rates and may even be amplified by the use of vacuum collection tubes. Our aim was to investigate the association of five different vacuum tubes with hemolysis rates in comparison to an aspiration system under real-life conditions and to propose an equation to estimate the amount of hemolysis, depending on the vacuum collection tube type. Methods: We retrospectively evaluated hemolysis data of plasma samples from our ED, where blood is drawn through IV catheters. Over the past 5 years, we compared 19,001 hemolysis index values amongst each other and against the respective vacuum pressure (Pv) of the collection tubes, which were used within the six observational periods. Results: The highest hemolysis rates were associated with full-draw evacuated tubes. Significantly reduced hemolysis was observed for two kinds of partial-draw tubes. The hemolysis rate of one partial-draw blood collection tube was comparable to those of the aspiration system. Regression analysis of Pv and mean free hemoglobin (fHb) values yielded the formula fHb (g/L)=0.0082*Pv2–0.1143*Pv+ 0.5314 with an R2 of 0.99. Conclusions: If IV catheters are used for blood collection, hemolysis rates directly correlate with the vacuum within the tubes and can be estimated by the proposed formula. By the use of partial-draw vacuum blood collection tubes, hemolysis rates in IV catheter collections can be reduced to levels comparable with collections performed by aspiration systems.

Colour coding for blood collection tube closures – a call for harmonisation

2015 ◽  
Vol 53 (3) ◽  
Author(s):  
Ana-Maria Simundic ◽  
Michael P. Cornes ◽  
Kjell Grankvist ◽  
Giuseppe Lippi ◽  
Mads Nybo ◽  
...  
Keyword(s):  
Decision Making ◽  
Hospital Care ◽  
Blood Collection ◽  
Laboratory Diagnostics ◽  
Laboratory Errors ◽  
Collection Tube ◽  
Patients Experience ◽  
Analytical Errors ◽  
Blood Collection Tube ◽  
Colour Coding

AbstractAt least one in 10 patients experience adverse events while receiving hospital care. Many of the errors are related to laboratory diagnostics. Efforts to reduce laboratory errors over recent decades have primarily focused on the measurement process while pre- and post-analytical errors including errors in sampling, reporting and decision-making have received much less attention. Proper sampling and additives to the samples are essential. Tubes and additives are identified not only in writing on the tubes but also by the colour of the tube closures. Unfortunately these colours have not been standardised, running the risk of error when tubes from one manufacturer are replaced by the tubes from another manufacturer that use different colour coding. EFLM therefore supports the worldwide harmonisation of the colour coding for blood collection tube closures and labels in order to reduce the risk of pre-analytical errors and improve the patient safety.

Evaluation of the BD Vacutainer® PST™ II Blood Collection Tube for special chemistry analytes

2009 ◽  
Vol 47 (3) ◽  
Author(s):  
Jeffrey Chance ◽  
Julie Berube ◽  
Marita Vandersmissen ◽  
Norbert Blanckaert
Keyword(s):  
Luteinizing Hormone ◽  
Follicle Stimulating Hormone ◽  
Thyroid Stimulating Hormone ◽  
Initial Time ◽  
Blood Collection ◽  
Time Intervals ◽  
Collection Tube ◽  
Total Thyroxine ◽  
Blood Collection Tube ◽  
Stimulating Hormone

Abstract: The performance of the BD Vacutainer: Tubes were drawn by routine venipuncture from 42 subjects according to a randomized draw order. Tubes were processed and centrifuged according to recommended handling procedures. Serum and plasma from the comparison tubes were aliquoted to secondary containers prior to analysis. Specimens were then tested for selected special chemistry analytes at two time intervals (initial time and after 24 h storage). Analytes tested included thyroid stimulating hormone, free thyroxine, total thyroxine, follicle stimulating hormone, luteinizing hormone, ferritin, cortisol, vitamin B12, folate, and testosterone. The data were collected and analyzed by analysis of variance and mean bias comparisons.: The performance of the BD Vacutainer: The BD VacutainerClin Chem Lab Med 2009;47:358–61.

scholarly journals Interlaboratory comparison of the TransFix®/EDTA Vacuum Blood Collection tube with the 5 mL Cyto‐Chex® BCT

2018 ◽  
Vol 96 (6) ◽  
pp. 496-507 ◽  
Author(s):  
Daniel Harrison ◽  
Rosalie Ward ◽  
Sarah Bastow ◽  
Andrew Parr ◽  
Susan Macro ◽  
...  
Keyword(s):  
Interlaboratory Comparison ◽  
Blood Collection ◽  
Collection Tube ◽  
Blood Collection Tube

Residual Negative Pressure in Vacuum Blood-Collection Tube and Hemolysis in Pediatric Blood Specimens

2019 ◽  
Vol 51 (1) ◽  
pp. 41-46
Author(s):  
Jing Hu ◽  
Qiao-Xin Zhang ◽  
Tong-Tong Xiao ◽  
Mei-Chen Pan ◽  
Ying-Mu Cai
Keyword(s):  
Negative Pressure ◽  
Blood Collection ◽  
Free Hemoglobin ◽  
Significant Difference ◽  
Collection Tube ◽  
Biochemical Analyzer ◽  
Blood Specimens ◽  
Adult Volunteers ◽  
Blood Collection Tubes ◽  
Blood Collection Tube

ABSTRACT Objective To determine a method to reduce specimen hemolysis rates in pediatric blood specimens. Methods A total of 290 blood specimens from pediatric patients were classified into the capped group or uncapped group. The hemolysis index and levels of lactate dehydrogenase (LDH) were measured using an automated biochemical analyzer. Also, we performed a paired test to measure the concentration of free hemoglobin in specimens from 25 randomly selected healthy adult volunteers, using a direct spectrophotometric technique. Results The hemolytic rate of capped specimens was 2-fold higher than that of uncapped specimens. We found significant differences for LDH. Also, there was a significant difference in the concentration of free hemoglobin in the random-volunteers test. Conclusions Eliminating the residual negative pressure of vacuum blood-collection tubes was effective at reducing the macrohemolysis and/or microhemolysis rate.

A Multicenter Evaluation of a Nongel Mechanical Separator Plasma Blood Collection Tube for Testing of Selected Therapeutic Drugs

2020 ◽  
Vol 5 (4) ◽  
pp. 671-685
Author(s):  
Svetlana Morosyuk ◽  
Julie Berube ◽  
Robert Christenson ◽  
Alan H B Wu ◽  
Denise Uettwiller-Geiger ◽  
...  
Keyword(s):  
The Other ◽  
Initial Time ◽  
Therapeutic Drug ◽  
Blood Collection ◽  
Test Results ◽  
Sample Storage ◽  
Therapeutic Drugs ◽  
Collection Tube ◽  
And Storage ◽  
Blood Collection Tube

Abstract Background Some therapeutic drugs are unstable during sample storage in gel tubes. BD Vacutainer® Barricor™ Plasma Blood Collection Tube with nongel separator was compared with plasma gel tubes, BD Vacutainer PST™, PST II, and BD Vacutainer Serum Tube for acetaminophen, salicylate, digoxin, carbamazepine, phenytoin, valproic acid, and vancomycin during sample storage for up to 7 days. Methods Seven hospital sites enrolled 705 participants who were taking at least one selected drug. The study tubes were collected and tested at initial time (0 h), after 48 h of storage at room temperature and on day 7 (after additional 5 days of refrigerated storage). The performance of BD Barricor tube was evaluated for each drug by comparing BD Barricor samples with samples from the other tubes at 0 h from the same participant; stability was evaluated by comparing test results from the same tube at 0 h, 48 h, and 7 days. Results At 0 h, BD Barricor showed clinically equivalent results for selected therapeutic drugs compared with the other tubes, except phenytoin in BD PST. Phenytoin samples ≥20 µg/mL in BD PST had 10–12% lower values than samples in BD Barricor. During sample storage, all selected drugs remained stable for 7 days in BD Barricor and in serum aliquots. In BD PST, all drugs remained stable except phenytoin and carbamazepine and in BD PST II except for phenytoin. Conclusion The BD Barricor Tube is effective for the collection and storage of plasma blood samples for therapeutic drug monitoring without sample aliquoting.

Non-invasive fetal RHD genotyping for RhD negative women stratified into RHD gene deletion or variant groups: comparative accuracy using two blood collection tube types

Pathology ◽  
2017 ◽  
Vol 49 (7) ◽  
pp. 757-764 ◽  
Author(s):  
Catherine A. Hyland ◽  
Glenda M. Millard ◽  
Helen O'Brien ◽  
Elizna M. Schoeman ◽  
Genghis H. Lopez ◽  
...  
Keyword(s):  
Gene Deletion ◽  
Blood Collection ◽  
Non Invasive ◽  
Collection Tube ◽  
Comparative Accuracy ◽  
Blood Collection Tube

scholarly journals A simple metric to quantify seismicity clustering

2010 ◽  
Vol 17 (4) ◽  
pp. 293-302 ◽  
Author(s):  
N. F. Cho ◽  
K. F. Tiampo ◽  
S. D. Mckinnon ◽  
J. A. Vallejos ◽  
W. Klein ◽  
...  
Keyword(s):  
Physical Meaning ◽  
Finite Time ◽  
Metastable Equilibrium ◽  
Lower Sensitivity ◽  
Time Interval ◽  
Finite Time Interval ◽  
Time Intervals ◽  
The Past ◽  
Local Clustering ◽  
Over Time

Abstract. The Thirulamai-Mountain (TM) metric was first developed to study ergodicity in fluids and glasses (Thirumalai and Mountain, 1993) using the concept of effective ergodicity, where a large but finite time interval is considered. Tiampo et al. (2007) employed the TM metric to earthquake systems to search for effective ergodic periods, which are considered to be metastable equilibrium states that are disrupted by large events. The physical meaning of the TM metric for seismicity is addressed here in terms of the clustering of earthquakes in both time and space for different sets of data. It is shown that the TM metric is highly dependent not only on spatial/temporal seismicity clustering, but on the past seismic activity of the region and the time intervals considered as well, and that saturation occurs over time, resulting in a lower sensitivity to local clustering. These results confirm that the TM metric can be used to quantify seismicity clustering from both spatial and temporal perspectives, in which the disruption of effective ergodic periods are caused by the agglomeration of events.

scholarly journals It's Time for a Better Blood Collection Tube to Improve the Reliability of Glucose Results

Diabetes Care ◽  
2012 ◽  
Vol 36 (1) ◽  
pp. e2-e2 ◽  
Author(s):  
M. J. Peake ◽  
D. E. Bruns ◽  
D. B. Sacks ◽  
A. R. Horvath
Keyword(s):  
Blood Collection ◽  
Collection Tube ◽  
Blood Collection Tube
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