scholarly journals Partitioning of cerrena unicolor laccase activity in an aqueous two-phase system

2016 ◽  
Vol 37 (2) ◽  
pp. 269-280 ◽  
Author(s):  
Michał Blatkiewicz ◽  
Axel Prinz ◽  
Andrzej Górak ◽  
Stanisław Ledakowicz
Keyword(s):  
Volume Ratio ◽  
Line Length ◽  
Bottom Phase ◽  
Phase System ◽  
Phase Volume ◽  
Two Phase ◽  
Aqueous Two Phase System ◽  
Phase Volume Ratio ◽  
Cerrena Unicolor ◽  
Tie Line

Abstract Culture supernatant containing laccase produced by Cerrena unicolor strain was used to examine laccase partitioning between phases in an aqueous two-phase system. The investigated system consisted of polyethylene glycol 3000 and sodium phosphate buffer adjusted to pH = 7. Influence of several parameters on partitioning was measured, including phase forming components’ concentrations, tie line lengths, phase volume ratio, supernatant dilution, process temperature and halogen salt supplementation. Partitioning coefficients up to 78 in the bottom phase were achieved with yields of over 90%. Tie line length and phase volume ratio had significant effect on enzyme partitioning.

scholarly journals Optimisation of LS54/Dx Aqueous Two Phase System Conditions for Cutinase Recovery

2013 ◽  
Vol 12 (2) ◽  
pp. 61
Author(s):  
Jamaliah Md. Jahim ◽  
Abdul Wahab Mohamad ◽  
Fariza Akmal Abdul Mutalib ◽  
Farah Diba Abu Bakar ◽  
Osman Hassan
Keyword(s):  
Recovery Process ◽  
Line Length ◽  
Phase System ◽  
Two Phase ◽  
Rich Phase ◽  
Aqueous Two Phase System ◽  
System Composition ◽  
Protein Partitioning ◽  
Tie Line ◽  
Positive Electrostatic Potential

Aqueous two phase system comprising Dehypon® LS 54 and K4484 Dextrin® was selected for recovery of cutinase enzyme. Parameters such as pH, system composition and type of salt as an additive, influenced the protein partitioning behaviour and optimisation of these parameters become necessary to be done in the design of primary recovery process of ATPS. The cutinase partitioning experiments were carried out with 30% of cutinase solution added to LS 54/Dx system. Results showed that cutinase enzyme preferred to partition into LS 54 rich-phase at pH 8.0 and the affinity of cutinase into top phase was observed higher with the increment of system compositions, which represented by tie line length (TLL). Furthermore, the addition of 50mM salts such as K2SO4 and KCl into LS 54/Dx system has led to raise partition coefficient of cutinase, kcut to 2.2 and 1.95 fold, respectively. The dependence of kcut on various additives such as (NH4)2SO4, Na2SO4 and K2SO4 at the same concentration, suggested that the addition of selected ions could enhance positive electrostatic potential which could attract more cutinase to partition into LS54 rich phase. As conclusion, the best conditions obtained for cutinase partitioning were pH8.0, TLL = 23% and Na2SO4 = 50mM, from which the maximum kcut of 2.83 with improved recovery of cutinase in top phase up to 79% can be achieved.

scholarly journals Pectinases partitioning in aqueous two-phase systems: An integration of the systems poly(ethylene glycol)crude dextran and poly(ethylene glycol)ammonium sulphate

2004 ◽  
Vol 69 (4) ◽  
pp. 299-307 ◽  
Author(s):  
Mirjana Antov ◽  
Draginja Pericin ◽  
Stana Pejin
Keyword(s):  
Ethylene Glycol ◽  
Total Concentration ◽  
Volume Ratio ◽  
Line Length ◽  
Salt System ◽  
Phase Volume ◽  
Poly Ethylene Glycol ◽  
Phase Volume Ratio ◽  
Poly Ethylene ◽  
Tie Line

The partitioning of pectinases in the poly(ethylene glucol)4000/ammonium sulpohate system was studied and also its application for enzymes extraction from the top phase of the poly(ethylene glucol)4000/crude dextran system. Almost complete one-sided partition of endo-pectinase and exo-pectinase to the bottom phase of the polymer/salt system was achieved at a tie-line length of 37.16 %. The concentration factors were 1.73 and 3.25, respectively. The highest total endo- and exo-pectinase yields (72.41 % and 69.46 % respectively) were obtained by integration of the polymer/polymer system at a tie-line of 8.61 % and a high phase volume ratio and the polymer/salt system at a tie-line of 30.23%and a low phase volume ratio. Integration of the partitioning at a high tie-line length in the polymer/polimer and a low tie-line length in the polymer/salt system resulted in a total concentration factor of 1.5 and a purification of 1.66 fold for exo-pectinase. The addition of phosphate to this integrated system improved the total concentration factor and purification fold of the activity to 1.73 and 2.14, respectively.

scholarly journals Extraction of tetracycline using the ionic liquid-based aqueous two phase systems: Single-stage versus multi-stage

2018 ◽  
Vol 24 (4) ◽  
pp. 387-397 ◽  
Author(s):  
Yi Liu ◽  
Li Chen ◽  
Jun Zhou ◽  
Zongcheng Yan
Keyword(s):  
Extraction Efficiency ◽  
Ph Value ◽  
Influence Factors ◽  
Volume Ratio ◽  
Line Length ◽  
Single Stage ◽  
Two Phase ◽  
Extraction Behavior ◽  
Multi Stage ◽  
Tie Line

Ionic liquids-based aqueous two-phase extraction (ILs-ATPE) offers an alternative approach to the extraction of tetracycline (TC) through their partitioning between two phases. Single-stage and multi-stage strategies have been evaluated and compared for the purification of TC using ATPE composed of 1-butyl-3-methylimidazolium halide ([Bmim]X(X=Cl,Br)) and K2HPO4. The influence factors on single-stage extraction behavior of TC were optimized systematically, including the pH value, tie line length, and volume ratio. The optimal extraction efficiency of TC could reach above 95% when the volume ratio is higher than 1.5 and the tie line length is 30.52%. The multi-stage ATPE was also investigated by simulating a three-stage crosscurrent operation in test tubes. According to the TC isotherm curve and respective McCabe?Thiele diagrams, a predicted optimized scheme of the countercurrent multi-stage ATPE was determined. TC can be purified in the IL-rich top phase with a final extraction efficiency of 99% and a final TC concentration of 0.25 mg/mL, if a three- -stage [Bmim]Cl-K2HPO4 ATPE with volume ratio of 0.5 and tie line length of 30.52% was employed. Thus, the multi-stage extraction with small volume ratio is necessary to achieve a higher recovery yield, resulting in the reduction of the IL consumption.

Method of computing hydraulic gradient for the case of a two-phase mixture flowing in sloping pipes

2019 ◽  
Author(s):  
A.S. Kondratiev ◽  
T.L. Nha
Keyword(s):  
Solid Phase ◽  
Slope Angle ◽  
Axisymmetric Flow ◽  
Volume Ratio ◽  
Hydraulic Gradient ◽  
Transitional Region ◽  
Phase Volume ◽  
Two Phase ◽  
Phase Volume Ratio ◽  
Phase Mixture

The paper investigates two-phase mixture flows in sloping pipes employing two computational methods in the transitional region of pipe slope angle. We used methods of computing two-mixture flows in horizontal and vertical pipes as the basis for our equations. When flowing downwards through a sloping pipe, the solid phase volume ratio distribution changes most significantly: an axisymmetric flow through a vertical pipe transforms into a flow featuring a markedly non-uniform distribution of the solid phase along the vertical plane in the sloping pipe. When flowing upwards, the solid phase volume ratio profile is inversely transformed. Comparison of the experimental and computational data showed that the datasets are in a sufficiently good agreement. The computational method developed is semi-empirical and may be recommended for calculating hydraulic gradient in sloping pipes.

scholarly journals Fractionation of phycocyanin and carbohydrate from Spirulina platensis using ionic liquid-based aqueous two-phase system

2021 ◽  
Vol 1195 (1) ◽  
pp. 012039
Author(s):  
Y H Choi ◽  
S K Mah ◽  
Y S Ng ◽  
S Y Lee
Keyword(s):  
Ionic Liquid ◽  
Spirulina Platensis ◽  
High Speed ◽  
Operation Time ◽  
Bottom Phase ◽  
Phase System ◽  
Two Phase ◽  
Citrate Buffer ◽  
Aqueous Two Phase System ◽  
Potential Applications

Abstract Microalgae have gained considerable attention due to their high-value biomolecules and potential applications in the pharmaceutical, food, medical and cosmeceutical field. However, the conventional biorefinery process of microalgae are costly, energy-intensive, and time-consuming. Aqueous two-phase system (ATPS) has emerged as a potential technique for the separation and fractionation of biomolecules in the biorefinery field. This study proposed the application of ionic liquid (IL) based ATPS for the fractionation of high-value phycocyanin and carbohydrates from a crude extract of Spirulina platensis.The biomass was first lysed by a high-speed homogenization with a solid to liquid ratio (S/L) of 1:10 and an operation time of 5 min to achieve optimum yields of multiple products. Next, the feasibility of several IL-based ATPS with phosphate/citrate buffer was evaluated by investigating the effects of cation, anion, and alkyl chain’s length of ILs. Among the IL-based ATPS, the system comprising of 1-butyl-1-methylpyrrolidinium dicyanamide and citrate buffer demonstrated the optimum phycocyanin extraction efficiency of 83.26 ± 0.05% at the top phase and a concurrent recovery of 73.89±0.06% carbohydrate at the bottom phase. In this work, the IL-based ATPS performed better than conventional polymer-based ATPS. This work suggests that IL-based ATPS allows efficient fractionation of phycocyanin and carbohydrates.

A nonintrusive auto-transformer technique for measuring phase volume ratio in oil–water two-phase stratified flow

2006 ◽  
Vol 54 (1-2) ◽  
pp. 25-33
Author(s):  
Abdullah A. Kendoush ◽  
Ghanim K. Abdul-Sada ◽  
Banipal N. Yaqob
Keyword(s):  
Volume Ratio ◽  
Stratified Flow ◽  
Phase Volume ◽  
Two Phase ◽  
Phase Volume Ratio ◽  
Oil Water

Pepsin extraction from bovine stomach using aqueous two-phase systems: Molecular mechanism and influence of homogenate mass and phase volume ratio

2008 ◽  
Vol 873 (2) ◽  
pp. 133-138 ◽  
Author(s):  
Natalia Imelio ◽  
Analía Marini ◽  
Darío Spelzini ◽  
Guillermo Picó ◽  
Beatriz Farruggia
Keyword(s):  
Molecular Mechanism ◽  
Volume Ratio ◽  
Phase Volume ◽  
Two Phase ◽  
Phase Volume Ratio ◽  
Aqueous Two Phase Systems ◽  
Phase Systems
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