scholarly journals Genetic variation of silver fir progeny from Tisovik Reserve population determined via microsatellite and isozyme markers

2017 ◽  
Vol 59 (1) ◽  
pp. 45-58
Author(s):  
Ewa Maria Pawlaczyk ◽  
Alina Bączkiewicz ◽  
Katarzyna Buczkowska ◽  
Maria Anna Bobowicz
Keyword(s):  
Genetic Variation ◽  
Abies Alba ◽  
Unknown Origin ◽  
Equilibrium Analysis ◽  
Silver Fir ◽  
Isolated Population ◽  
Primeval Forest ◽  
High Level ◽  
Białowieża Primeval Forest ◽  
Progeny Line

Abstract Progeny from 19 family lines of silver fir (Abies alba Mill.) from a small, native and isolated population from the Tisovik Reserve (Belarusian part of Białowieża Primeval Forest) growing in an experimental plot near Hajnówka (Polish part of Białowieża Primeval Forest) were analysed in terms of 4 nuclear microsatellite DNA loci and 9 isozyme systems with 14 loci. The aim of this study was to determine the genetic variation within and between progeny lines. Analysis of isozyme loci showed that all progeny lines, except the progeny lines T6 and T16, were characterised by an excess of heterozygotes and 20% of the detected variation occurred between progeny. Progeny formed two groups. Microsatellite loci showed that 6 progeny lines demonstrated an excess of heterozygotes and 12 an excess of homozygotes. On an average, the population was in the Hardy-Weinberg equilibrium. Analysis of molecular variance (AMOVA) showed that 14% of the detected variation occurred between offspring and the remaining 86% within progeny lines. The most distinct progeny line was Tl, where the highest number of alleles per locus was detected. Generally, progeny of Tisovik is characterised by high level of differentiation as the offspring of isolated population that have limited number of individuals to crossing (only 20). In some progeny line, the private alleles that are detected may be the result of pollination from Polish part of Białowieża Forest where in 1920s and 1930s of XX century had planted the seedling of silver fir of unknown origin. The substructuring of population is observed, and the detected deficiency of heterozygotes may be ostensible as a result of the Wahlund effect. Such pattern of genetic structure could also be an effect of harsh environmental conditions exerting selection pressure and modifying the genetic composition of this population.

scholarly journals Cisovka - the relic population of Abies alba and its relationship to man-made silver-fir stands in Białowieża primeval forest

2014 ◽  
Vol 65 (3-4) ◽  
pp. 319-328 ◽  
Author(s):  
Leon Mejnartowicz
Keyword(s):  
Genetic Diversity ◽  
Genetic Distance ◽  
Abies Alba ◽  
Silver Fir ◽  
Low Grade ◽  
Primeval Forest ◽  
Genetic Pollution ◽  
Geographical Regions ◽  
Białowieża Primeval Forest ◽  
Relic Population

In Białowieża Primeval Forest, in 1823 Stanisław Górski discovered on the Cisovka Hag, a relic population of European silver-fir (<em>Abies alba</em> Mill.). This population is isolated and most away, 120 km to the North-East, from the border of European-silver-fir distribution. Besides the natural population Cisovka, there are also man-made silver fir stands and clumps in the Polish and Belorussian part of Białowieża Primeval Forest. In the Polish part there are four such artificial stands. If the seed-producing silver-fir stands really originated from the Cisovka population, then they are a very valuable part of the declining population and an easy accessible seed source. However, if these populations were introduced to the Białowieża Primeval Forest, then they are a potential source of dangerous genetic pollution of the Cisovka population. The relationship of the genetic structure of the Cisovka population to man-made silver-fir-stands in Białowieża Forest was investigated with the help of 17 loci of 1 1 enzyme systems. Genetic diversity of Cisovka population is characterized by the smallest mean number of alleles per locus (<em>Mal</em>= 1.353, includes all loci studied and per polimorphic locus <em>Malp</em> = 2.00). In Cisovka population there is very low-grade of polimorphic loci (<em>Pp</em> = 11.765 with the mean 37.255 for all studied populations). Expected heterozygosity, He = 0.079 revealed very low-grade of genetic diversity in the population. The observed heterozygosity (Ho = 0.123) was similar to this characterictic in other populations. A dendrogram based on Neis genetic distance coefficient (D) among 9 silver-fir populations was constructed. Cisovka in the UPGMA dendrogram is a distinct population separated from other ones by a very great genetic distance (D = 0.06). Also two man-made silver-fir (B I and 132) stands are separated from others. Only populations B3 and B4 are combined into one subgroup linked to the population Tomaszów Lubelski. Basing on the received results one should decide that all the four studied artificial stands were introduced to Białowieża Primeval Forest from beyond of this geographical regions. There is some reason to assume (on the GDH2 allele frequency) that man-made silver-stands in compartments 453Aa and 498Cg are of eastern provenance. It is rather certain that these stands are not of offspring of Cisovka populations.

scholarly journals The ‘Tisovik’ reserve of Silver fir in the Belarusian national park ‘Belavezhskaya Pushcha’

2015 ◽  
Vol 76 (1) ◽  
pp. 18-36
Author(s):  
Adolf F. Korczyk
Keyword(s):  
Genetic Structure ◽  
Plant Communities ◽  
National Park ◽  
Abies Alba ◽  
Silver Fir ◽  
Natural Origin ◽  
Primeval Forest ◽  
North East ◽  
The North ◽  
Białowieża Primeval Forest

Abstract Silver firs (Abies alba Mill.) of natural origin occurring in the Belorussian part of the Białowieża Primeval Forest constitute an enclave situated 120 km to the north-east of a dense fir stand in the Polish part of the forest. In order to protect this population a reserve called ‘Tisovik’ was established. In this study, plant communities occurring in the ‘Tisovik’ reserve were described and its fir population (20 trees) was characterised in terms of its genetic structure.

scholarly journals Variability of silver fir (Abies alba Mill.) progeny from the Tisovik Reserve expressed in needle traits and chloroplast microsatellite DNA

2017 ◽  
Vol 45 (1) ◽  
pp. 1-16
Author(s):  
Ewa M. Pawlaczyk ◽  
Maria A. Bobowicz
Keyword(s):  
Genetic Variation ◽  
Microsatellite Dna ◽  
Abies Alba ◽  
Silver Fir ◽  
Experimental Plot ◽  
Range Limit ◽  
Chloroplast Microsatellite ◽  
Natural Range ◽  
Resin Canals ◽  
Białowieża Primeval Forest

AbstractProgeny from nineteen family lines of silver fir (Abies alba Mill.) from the Tisovik Reserve growing in an experimental plot were analyzed based on 4 chloroplast microsatellite DNA loci and 12 morphological and anatomical needle traits. The Tisovik Reserve is located in Białowieża Primeval Forest, 120 km north of the natural range limit of this species, and embraces a small and isolated natural population of silver fir. The aim of this study was to determine genetic variation within and between progeny lines. Analysis of phenotypic variation showed that the traits which differed most among individuals were the needle width and the distance from resin canals to vascular bundle. Those traits, which differed most between the progeny lines, were the number of endodermic cells around the vascular bund and the weight of hypodermic cells. In Tisovik progeny, we detected 107 different haplotypes. In progeny lines, we detected more haplotypes than in maternal trees, and most haplotypes did not exist in maternal trees. This may be the result of pollen influx from other silver fir stands. Progeny from Tisovik showed a higher level of variability in comparison with maternal trees.

scholarly journals Genetic analysis of silver-fir populations in the Beskids

2011 ◽  
Vol 72 (2) ◽  
pp. 115-119 ◽  
Author(s):  
Leon Mejnartowicz
Keyword(s):  
Genetic Diversity ◽  
Genetic Variation ◽  
Genetic Analysis ◽  
Abies Alba ◽  
Genetic Distances ◽  
Silver Fir ◽  
Geographical Distance ◽  
Polymorphic Loci ◽  
Expected Heterozygosity ◽  
Rare Alleles

Twenty-eight isozymic loci were studied in the Beskid Mts., in four populations of common silver-fir (<em>Abies alba</em>): one in Beskid Makowski (BM) and three populations in Beskid Sądecki (BS). Their genetic variation and diversity were analyzed, and Nei's genetic distances between the populations were calculated. The results show that the geographical distance between the BM population and the three BS populations is reflected in genetic distances. The BM population is clearly distinct from the others. It has the lowest genetic diversity (<em>I</em> = <em>0.42</em>), percentage of polymorphic loci <em>(%PoL </em>= <em>64.29</em>) and number of rare alleles (<em>NoRa </em>= <em>5</em>). Besides, the BM population has the highest observed heterozygosity (<em>Ho </em>= <em>0.291</em>), which exceeds the expected heterozygosity (<em>He </em>= <em>0.254</em>), estimated on the basis of the Hardy-Weinberg Principle. On the contrary, BS populations are in the state of equilibrium, which is manifested, in similar values of <em>He </em>= <em>0.262 </em>and <em>Ho </em>= <em>0.264</em>.

Levels of genetic variation in European silver fir (Abies alba)

Genetica ◽  
1990 ◽  
Vol 82 (1) ◽  
pp. 1-10 ◽  
Author(s):  
F. Bergmann ◽  
H. -R. Gregorius ◽  
J. B. Larsen
Keyword(s):  
Genetic Variation ◽  
Abies Alba ◽  
Silver Fir

scholarly journals Forest decline has not reduced genetic diversity of naturally regenerated Norway spruce from the Beskids, Poland

2015 ◽  
Vol 64 (1-6) ◽  
pp. 270-278 ◽  
Author(s):  
Elżbieta Chomicz-Zegar ◽  
J. A. Nowakowska ◽  
A. Tereba
Keyword(s):  
Genetic Diversity ◽  
Genetic Variation ◽  
Norway Spruce ◽  
Forest Decline ◽  
Gene Diversity ◽  
Young Generation ◽  
Primeval Forest ◽  
Initial Stage ◽  
Spruce Stands ◽  
High Level

AbstractNorway spruce forests decline in the Beskids raises concerns about reduction of genetic variation in a next generation of forest. We aim to determine whether the genetic diversity in declining spruce stands was successfully transmitted to its self-seeded progeny. Using three nuclear microsatellite markers, we carried out the genetic assessment of naturally regenerated progeny and compered them with their maternal stands at an initial stage of decline. We investigated three spruce stands from the Śląski and Żywiecki Beskid and one primeval forest from the Tatras, as a reference. We noticed a high level of gene diversity, allelic richness and an effective number of alleles in progeny of all stands, without differences between declining stands from the Beskids and the primeval forest. The gene diversity was similar in maternal trees and their offspring. Higher gene diversity and generally higher number of alleles were noticed in young generation of stands. Genetic identity between generations was high in analyzed seed stands and the highest in the primeval forest. The results we presented indicate no significant effect of drastic reduction in the population size due to the forest decline on the level of genetic variation in self-seeded progeny of Norway spruce from the Beskids.

scholarly journals Variability of stomatal conductance in a small and isolated population of silver fir (Abies alba Mill.)

2011 ◽  
Vol 31 (5) ◽  
pp. 500-507 ◽  
Author(s):  
P. Piovani ◽  
S. Leonardi ◽  
F. Magnani ◽  
P. Menozzi
Keyword(s):  
Stomatal Conductance ◽  
Abies Alba ◽  
Silver Fir ◽  
Isolated Population

scholarly journals Keystone role of Eurasian beaver Castor fiber in creating the suitable habitat over the core breeding range for forest specialist species the three-toed woodpecker Picoides tridactylus

2019 ◽  
Vol 25 (2) ◽  
pp. 223-227 ◽  
Author(s):  
Krzysztof Pietrasz ◽  
Dawid Sikora ◽  
Tomasz Chodkiewicz ◽  
Magdalena Ślęzak ◽  
Bartłomiej Woźniak
Keyword(s):  
Abies Alba ◽  
Breeding Site ◽  
Distribution Range ◽  
Castor Fiber ◽  
Silver Fir ◽  
Suitable Habitat ◽  
Breeding Range ◽  
Eurasian Beaver ◽  
High Level

The European breeding range of the three-toed woodpecker Picoides tridactylus overlaps with the distribution range of coniferous and mixed forests with domination of Norway spruce Picea abies or European silver fir Abies alba. The paper describes a new breeding site of the three-toed woodpecker in the Sobibór Forest, eastern Poland outside the distribution range of these two tree species and its relationship with Eurasian beaver Castor fiber. The birds occurred in dead alder forests and were recorded 3–4 years after forest started to decline due to the high level of surface waters associated with the keystone role of the beaver. The decaying cycle of trees was possible because the area settled by woodpeckers was protected as a nature reserve.

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