scholarly journals The effect of cycloheximide and growth regulators on the senescence of cut leaves in Hosta sp. and Zantedeschia aethiopica

2015 ◽  
Vol 27 (2) ◽  
pp. 175-182 ◽  
Author(s):  
Ewa Skutnik ◽  
Julita Rabiza-Świder ◽  
Agata Jędrzejuk ◽  
Diana Musiał
Keyword(s):  
Growth Regulators ◽  
Cysteine Protease ◽  
Membrane Integrity ◽  
Vase Life ◽  
Free Proline ◽  
Protein Synthesis Inhibitors ◽  
Zantedeschia Aethiopica ◽  
Postharvest Treatment ◽  
Cut Foliage ◽  
Cut Leaves

Abstract The leaves of Zantedeschia and Hosta are used as florist greens in different floral arrangements. The most efficient postharvest treatment for cut foliage is the use of growth regulators, which prolong their vase life by delaying degradative changes occurring in leaves, especially proteolysis. Cycloheximide (CHI) is one of the protein synthesis inhibitors, blocking the enzymes responsible for decreasing membrane integrity, a phenomenon hastening senescence. The aim of this experiment was to evaluate the effects of CHI and benzyladenine (BA) or gibberellic acid (GA3) on the longevity of cut foliage in hosta (Hosta sp.) cultivars and Ethiopian calla (Zantedeschia aethiopica) and to follow the changes in certain proteolytic processes occurring during senescence. Generally, 24 h conditioning with cycloheximide shortened the longevity of cut calla leaves while having no effect on hosta vase life. In ageing leaves of ‘Minima Glauca’ hosta and calla, the total proteolytic activity increased, including that of cysteine protease. Due to the application of BA or GA3 in hosta and calla, respectively, this activity was limited. On the contrary, the use of CHI either did not affect the activity of cysteine protease or increased it several-fold relative to the control, in hosta and calla, respectively. Leaves treated with growth regulators had many more soluble proteins and fewer free amino acids, including free proline, than leaves from other treatments. The highest free proline level was determined in calla leaves conditioned with CHI, where it increased 18-fold relative to the initial level.

scholarly journals The Effect of a Sugar-Containing Preservative on Senescence-Related Processes in Cut Clematis Flowers

2018 ◽  
Vol 47 (2) ◽  
pp. 432-440 ◽  
Author(s):  
Julita RABIZA-ŚWIDER ◽  
Ewa SKUTNIK ◽  
Agata JĘDRZEJUK
Keyword(s):  
Proteolytic Activity ◽  
Cysteine Protease ◽  
Reducing Sugars ◽  
Soluble Proteins ◽  
Flower Senescence ◽  
Vase Life ◽  
Free Proline ◽  
Cut Flower ◽  
Cut Flowers ◽  
Life Itself

Clematis is a new species grown as cut flower, whose vase life is variable and cultivar-depended. Little is known about senescence of its cut flowers and their response to flower preservatives. The aim of the study was to evaluate the effect of a preservative (standard preservative SP, 8-hydroxyquinoline citrate plus sucrose) or a biocide solution (8-HQC) on certain senescence-related processes in cut clematis flowers. Analyses were done immediately after harvest and at the end of the vase life when control flowers held in water were wilting. A possible relationship between senescence parameters and the vase life of clematis taxa was also sought. As in most cut flowers, the contents of reducing sugars and soluble proteins in clematis petals decreased during the vase life while the proteolytic activity, including that of the cysteine protease, increased and was accompanied by accumulation of free proline and ammonium. Cut flower longevity in cultivars under study was not associated with the initial levels of reducing sugars, soluble proteins or free proline. Neither was the initial proteolytic activity or its increase during vase life related to the vase life itself: cultivars having comparable life spans differed dramatically in the initial and final proteolytic activities. Both solutions containing 8-HQC significantly affected the senescence-related processes and flowers held in them had more soluble proteins and lower proteolytic activity (total, and that of the cysteine protease) than control flowers held in water. Approximately a twofold increase in reducing sugars was observed in flowers held in SP relative to those held in water or in 8-HQC while the accumulation of free proline and ammonium was limited in their petals. This suggests a regulating action of exogenous sugar in senescence of clematis flowers. However, the delay of senescence produced by the preservative was not always associated with a longer vase life in any given cultivar. Further studies are needed to elucidate the role of sugar in clematis flower senescence.

scholarly journals Effect of 5-Aminolevulinic Acid (ALA) on the Biochemical and Physiological Parameters of Postharvest Quality of Polygonatum multiflorum L. All. ‘Variegatum’ Cut Foliage

Agronomy ◽  
2020 ◽  
Vol 10 (10) ◽  
pp. 1502
Author(s):  
Katarzyna Rubinowska ◽  
Elżbieta Pogroszewska ◽  
Paweł Szot
Keyword(s):  
Aminolevulinic Acid ◽  
Foliar Application ◽  
Postharvest Quality ◽  
Vase Life ◽  
Chl A ◽  
Plant Cultivation ◽  
Cut Foliage ◽  
Cut Leaves ◽  
Cultivation Site

Due to the dynamic development of the floriculture market, new species and varieties of plants that can potentially be used as a source of cut greenery are constantly being sought. The experiment was conducted to analyze the effect of the cultivation site (unheated foil tunnel and open field) and treatments with the Pentakeep V formulation (0.00%, 0.02%, 0.04%, and 0.06%) on the vase life and postharvest quality of Polygonatum multiflorum ‘Variegatum’ cut foliage. There was a positive effect of the cultivation in the unheated foil tunnel on the vase life and biochemical parameters, namely the electrolyte leakage (EL), thiobarbituric acid reactive substances (TBARS), chlorophyll a + b (Chl. a + b), proline, peroxidase (POD), and ascorbate peroxidase (APX), of P. multiflorum cut foliage. Foliar application of Pentakeep V formulation at concentrations of 0.04% and 0.06% contributed to a decrease in the TBARS content in the P. multiflorum cut leaves, regardless of the plant cultivation site. Additionally, it reduced proline production in cut leaves of P. multiflorum cultivated in the unheated foil tunnel, which was confirmed by the analyses carried out after the 30 days of the experiment. Furthermore, the exogenous application of ALA during P. multiflorum cultivation inhibited POD activity, irrespective of its concentration and the site of plant cultivation.

scholarly journals Production and Postharvest Treatment of Cut Stems of Eucalyptus L. Hér. Foliage

HortScience ◽  
1996 ◽  
Vol 31 (6) ◽  
pp. 1007-1009 ◽  
Author(s):  
Michelle G. Wirthensohn ◽  
Margaret Sedgley ◽  
Renate Ehmer
Keyword(s):  
Eucalyptus Globulus ◽  
Short Pulse ◽  
Ground Level ◽  
Vase Life ◽  
Above Ground Level ◽  
Dry Storage ◽  
Postharvest Treatment ◽  
Cut Foliage ◽  
Old Trees ◽  
Long Pulse

Optimum pruning height for cut foliage production was investigated for 3-year-old trees of Eucalyptus globulus Labill. Trees cut at a height of 1.0 m above ground level had most stems resprouting from the trunk, but a pruning height of 0.5 m produced the longest stems. Postharvest trials were conducted to assess the vase life of cut stems, and the effect of pulsing and simulated transportation on vase life. Holding solutions containing 1% or 2% sucrose and 8-HQC at 200 mg·L–1 significantly increased vase life of E. globulus and E. cinerea F. Muell. ex Benth. over the control, but pulsing E. cinerea in 1%, 5%, or 10% sucrose plus 8-HQC for 2 hours at 24 °C or 24 hours at 3 °C had no effect. In simulated transport trials, pulsing overnight in 1% or 5% sucrose plus 8-HQC at 3 °C followed by 1 week dry storage at 3 °C had no effect on the vase life of cut stems of E. sideroxylon Cunn. ex Wools., E. platypus Hook., E. spathulata Hook., E. cladocalyx F. Muell. E. platypus, or E. spathulata E. sargentii Maiden, but a 5% sucrose pulse plus 8-HQC significantly increased the vase life of E. spathulata E. platypus. A long pulse at low temperature (24 hours/3 °C) followed by 1 week dry storage was more effective than a short pulse at high temperature (2 hours/24 °C) for E. albida Maiden & Blakely stems and no sucrose was more effective than 1% or 5%. Thus, a pruning height of 0.5 or 1.0 m was optimum for cut foliage production of E. globulus, and a 2% sucrose holding solution extended vase life. There was no advantage of sucrose pulsing to extend vase life, or to improve vase life following dry storage, except for the hybrid E. spathulata E. platypus. Chemical name used: 8-hydroxyquinoline citrate (8-HQC).

Effect of Plant Growth Regulators on Growth and Quality Flower Production of Chrysanthemum (Chrysanthemum Indicum L.)

2022 ◽  
Vol 2 (1) ◽  
pp. 10-18
Author(s):  
Md Ehsanullah ◽  
Ahasan Ullah Khan ◽  
Md Kamruzzam ◽  
Sarah Tasnim
Keyword(s):  
Plant Growth ◽  
Plant Growth Regulators ◽  
Growth Regulators ◽  
Block Design ◽  
Research Centre ◽  
Vase Life ◽  
Flower Initiation ◽  
Growth Promoter ◽  
Flower Production ◽  
Minimum Number

A field study was conceded to assess the effect of plant growth regulators on growth and quality flower production of chrysanthemum at Horticulture Research Centre (HRC), Gazipur, Bangladesh. The experiment was laid out in Randomized Complete Block Design (RCBD) with ten (10) treatments and three replications. The treatments of plant growth regulators concentration were T1-50 ppm GA3, T2-100 ppm GA3, T3-150 ppm GA3, T4-400 ppm CCC, T5-600 ppm CCC, T6-800 ppm CCC, T7-250 ppm MH, T8-500 ppm MH, T9-750 ppm MH and, T10-Control. The maximum spreading of plant (27.0 cm) was observed when plants were treated with GA3 @ 150 ppm where the minimum plant spread (16.8 cm) was recorded in plants treated with CCC @ 800 ppm. The higher number of suckers (33) per pot was produced when pots were treated with GA3 @ 150 ppm whereas, application of CCC at three different concentrations produced lower number of suckers.  The highest number of flower (40) was recorded with 150 ppm GA3, where minimum number of flowers (25) per pot in 800 ppm CCC. The plants sprayed with 50 ppm GA3 took 48 days to flower initiation, whereas, it took 70 days with 750 ppm MH. the highest plants recorded (7.40 cm) with 800 ppm CCC, whereas, lowest size (6.50 cm) was obtained with the application of 500 ppm MH. The maximum vase life of flowers was recorded for the treatment 800 ppm CCC (15 days), which was at par with 13 days vase life obtained by spraying 600 ppm CCC. Therefore, it is concluded that the GA3 acted as growth promoter and the CCC acted as growth retardants on yield and quality of chrysanthemum.

The synergistic effect of maleic acid hydrazide (1.2-dihydro-3,6-pyridazinedione) and sucrose on vase life of cut roses

2002 ◽  
Vol 42 (5) ◽  
pp. 637
Author(s):  
K.-L. Huang ◽  
L.-J. Liao ◽  
R.-S. Shen ◽  
W.-S. Chen ◽  
Y.-H. Lin
Keyword(s):  
Synergistic Effect ◽  
Water Uptake ◽  
Water Loss ◽  
Maleic Acid ◽  
Ethylene Production ◽  
Acid Hydrazide ◽  
Vase Life ◽  
Cut Flowers ◽  
Postharvest Treatment ◽  
Cut Rose

Continuous postharvest treatment of cut rose flowers (Rosa hybrida L. cv. Diana) with maleic acid hydrazide (1.2-dihydro-3,6-pyridazinedione, MH) at 560.5 8-hydroxyquinoline sulfate (HQS) at 388.4 HQS, MH + HQS or sucrose + HQS treatments. The longevity of flowers in MH + sucrose in combination with HQS was extended for 18 days after vase treatments, whereas the longevity of cut flowers was only 4, 6 and 8 days for HQS, MH + HQS and sucrose + HQS, respectively. Cut roses treated with MH + sucrose + HQS in vase solution exhibited greater water uptake and less water loss than those in HQS. The concentrations of various sugars in petals were highest in the sucrose + HQS treatment, and MH + sucrose + HQS > MH + HQS > HQS. Ethylene production was significantly lower in sucrose + HQS or MH + sucrose + HQS treatments in comparison to MH + HQS, or HQS.

VASE LIFE OF NEW TROPICAL CUT FOLIAGE: CORDYLINE TERMINALIS

2012 ◽  
pp. 351-356 ◽  
Author(s):  
B.T. Favero ◽  
Q.A.C. Carmello ◽  
G.M. Dias
Keyword(s):  
Vase Life ◽  
Cut Foliage

Characterisation of xylem conduits and their possible role in limiting the vase life of cut Acacia holosericea (Mimosaceae) foliage stems

2011 ◽  
Vol 38 (7) ◽  
pp. 614 ◽  
Author(s):  
Jilushi W. Damunupola ◽  
Kamani Ratnayake ◽  
Daryl C. Joyce ◽  
Donald E. Irving
Keyword(s):  
Water Flow ◽  
Vase Life ◽  
Cut Flowers ◽  
Test Species ◽  
Vessel Elements ◽  
Acacia Holosericea ◽  
Cut Foliage ◽  
Perforation Plates ◽  
Vestured Pits ◽  
Xylem Conduits

Early desiccation limits the vase life of Acacia cut flowers and foliage and may be attributable to poor hydraulic conductivity (Kh) of the cut stems. Acacia holosericea A.Cunn. ex G.Don has been adopted as the test species to investigate the postharvest water relations of the genus Acacia. To understand potential constraints on Kh, xylem conduits in cut A. holosericea stems were anatomically characterised by light and scanning and transmission electron microscopy. Vessels with simple perforation plates and tracheids were the principal water conducting cells. Bordered vestured intervessel pits were present in xylem vessel elements. The majority of conduits (89%) were short at 1–5 cm long. Only 2% were 15–16 cm in length. Mean xylem conduit diameter was 77 ± 0.9 µm and the diameter profile showed a normal distribution, with 29% of diameters in the range of 70–80 µm. Simple perforation plates can offer relatively low resistance to water flow. On the other hand, bordered vestured pits and short xylem conduits can confer comparatively high resistance to water flow. Overall, the presence of bordered vestured pits, together with a high proportion of short xylem conduits and high stomatal densities (232 ± 2 mm–2) on unifacial phyllodes, could contribute to early dehydration of A. holosericea cut foliage stems standing in vase water. Further research will relate these anatomical features with changes in Kh and transpiration of cut foliage stems.

scholarly journals Study on waxing of cut foliage post harvest life of few ornamental species

2014 ◽  
Vol 6 (2) ◽  
pp. 619-624 ◽  
Author(s):  
Vivek Powar ◽  
Bhavya Bhargava ◽  
Ghumare Vikas
Keyword(s):  
Water Uptake ◽  
Ornamental Plants ◽  
Vase Life ◽  
Post Harvest ◽  
Maximum Water ◽  
Cut Foliage ◽  
Emulsion Concentration ◽  
Ornamental Species ◽  
Foliage Weight

Enhancing the vase life of cut foliages is one of the prime goals of floriculturist and it is very interesting and important aspect of floriculture.The efforts were made to find out best waxing treatment for enhancing the vase life along with excellent quality maintenance for few cut foliage species .The objective was to evaluate optimum percent wax solution for improving post harvest life and quality of ornamental plants. Different wax emulsion treatments (0.25, 0.50, 0.75 and 1.00 %) showed significant results in both quantitative and qualitative attributes of ornamental species. In Asparagus densiflorus ‘Sprengeri’ and Eucalyptus teritecornis,0.75% wax emulsion concentration recorded minimum foliage weight (5.62, 20.49 g) and minimum water uptake (5.60, 32.73 ml) respectively on 3rd day, while maximum foliage weight (0.90, 6.84 g) and maximum water uptake (1.13, 13.77 ml) was recorded on senescence day. In Caryota urens (Fishtail palm) 0.50% wax emulsion concentration recorded minimum foliage weight (9.71 g), minimum water uptake (6.53 ml) on 3rdday, while maximum foliage weight(1.83 g) and maximum water uptake (1.20 ml) on senescence day. In Heliconia psittachroum cv. Golden Torch, 1.00 % wax emulsion concentration recorded minimum foliage weight (28.85 g), minimum water uptake (20.35 ml) on 3rdday, whereas maximum foliage weight (15.83 g)and maximum water uptake (5.75 ml) on senescence day. There was improvement and increament in post harvest quality and vase life of studied ornamental cut foliages.

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