Intensification of β-glucosidase enzyme production from Aspergillus niger using extractive fermentation with an aqueous two-phase system

2017 ◽  
Vol 6 (4) ◽  
Author(s):  
Avinash B. Dhage ◽  
Virendra K. Rathod
Keyword(s):  
Aspergillus Niger ◽  
Wheat Bran ◽  
Enzyme Production ◽  
Phase System ◽  
Extractive Fermentation ◽  
Two Phase ◽  
Aqueous Two Phase System ◽  
Main Substrate

AbstractAn extractive fermentation approach in an aqueous two-phase system (ATPS) with wheat bran as a main substrate was used in this study to intensify the production of β-glucosidase enzyme (EC 3.2.1.21) from

Partition in aqueous two-phase system: Its application in downstream processing of tannase from Aspergillus niger

2013 ◽  
Vol 101 ◽  
pp. 392-397 ◽  
Author(s):  
Luis V. Rodríguez-Durán ◽  
Darío Spelzini ◽  
Valeria Boeris ◽  
Cristóbal N. Aguilar ◽  
Guillermo A. Picó
Keyword(s):  
Aspergillus Niger ◽  
Downstream Processing ◽  
Phase System ◽  
Two Phase ◽  
Aqueous Two Phase System

scholarly journals Purification of polygalacturonases produced by Aspergillus niger using an aqueous two-phase system

2014 ◽  
Vol 371 ◽  
pp. 125-130 ◽  
Author(s):  
Marília de H.C. Maciel ◽  
Cristiane A. Ottoni ◽  
Polyanna N. Herculano ◽  
Tatiana S. Porto ◽  
Ana L.F. Porto ◽  
...  
Keyword(s):  
Aspergillus Niger ◽  
Phase System ◽  
Two Phase ◽  
Aqueous Two Phase System

Extractive fermentation of clavulanic acid by Streptomyces DAUFPE 3060 using aqueous two-phase system

2010 ◽  
Vol 27 (1) ◽  
pp. 95-103 ◽  
Author(s):  
D. A. Viana Marques ◽  
A. Pessoa-Júnior ◽  
J. L. Lima-Filho ◽  
A. Converti ◽  
P. Perego ◽  
...  
Keyword(s):  
Clavulanic Acid ◽  
Phase System ◽  
Extractive Fermentation ◽  
Two Phase ◽  
Aqueous Two Phase System

Effect of aeration and agitation on extractive fermentation of clavulanic acid by using aqueous two-phase system

2016 ◽  
Vol 32 (6) ◽  
pp. 1444-1452 ◽  
Author(s):  
Daniela A. Viana Marques ◽  
Valéria C. Santos-Ebinuma ◽  
Adalberto Pessoa-Júnior ◽  
Ana L. F. Porto ◽  
Beatriz Rivas Torres ◽  
...  
Keyword(s):  
Clavulanic Acid ◽  
Phase System ◽  
Extractive Fermentation ◽  
Two Phase ◽  
Aqueous Two Phase System

scholarly journals Extractive Fermentation for Recovery of Bacteriocin-Like Inhibitory Substances Derived from Lactococcus lactis Gh1 Using PEG2000/Dextran T500 Aqueous Two-Phase System

Fermentation ◽  
2021 ◽  
Vol 7 (4) ◽  
pp. 257
Author(s):  
Roslina Jawan ◽  
Sahar Abbasiliasi ◽  
Joo Shun Tan ◽  
Murni Halim ◽  
Shuhaimi Mustafa ◽  
...  
Keyword(s):  
Lactococcus Lactis ◽  
Continuous Production ◽  
Scale Up ◽  
Downstream Processing ◽  
Sustainable Growth ◽  
Phase System ◽  
Extractive Fermentation ◽  
Cell Cultivation ◽  
Two Phase ◽  
Aqueous Two Phase System

This work aimed to optimize the parameters affecting partitioning of a bacteriocin-like inhibitory substances (BLIS) from Lactococcus lactis Gh1 in extractive fermentation using polyethylene glycol (PEG)/dextran aqueous two-phase system (ATPS). This system was developed for the simultaneous cell cultivation and downstream processing of BLIS. Results showed that the molecular weight of PEG, PEG concentration, and dextran T500 affect the partition coefficient (K), purification factor (PF), and yield of BLIS partitioning. ATPS composed of 10% (w/w) PEG2000 and 8% (w/w) dextran T500, provided the greatest conditions for the extractive BLIS production. The K (1.00 ± 0.16), PF (2.92 ± 0.37) and yield (77.24 ± 2.81%) were increased at selected orbital speed (200 rpm) and pH (pH 7). Sustainable growth of the cells in the bioreactor and repeated fermentation up to the eighth extractive batch were observed during the scale up process, ensuring a continuous production and purification of BLIS. Hence, the simplicity and effectiveness of ATPS in the purification of BLIS were proven in this study.

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