Validation of Microsatellite Markers of Pl Resistance Genes to Downy Mildew of Sunflower

Helia ◽  
2018 ◽  
Vol 41 (68) ◽  
pp. 73-82
Author(s):  
A. Solodenko
Keyword(s):  
Downy Mildew ◽  
Ssr Markers ◽  
Resistance Genes ◽  
Microsatellite Loci ◽  
Marker Assisted Selection ◽  
Parental Line ◽  
Gene Control ◽  
Genetically Determined ◽  
Ssr Polymorphism ◽  
Simple Sequence

AbstractSimple sequence repeats (SSR) polymorphism of 34 microsatellite loci (LG1, 8 and 13) was studied in lines carrying the downy mildew resistance genes Pl and lines with no Pl. The microsatellite loci ORS328 and ORS781 were selected as markers for genes Pl6 and Pl8 in lines HA 335 and QHP-1, respectively. Markers were identified for gene PlARG in RHA 419 and some accessions of H. argophyllus. The SSR markers ORS509, ORS605, ORS610, ORS1182 and ORS1039 were proven to reliably identify the parental line carrying PlARG gene, control and select the heterozygous F1 hybrids and identify homozygous genotypes in F2 generations. Obtained results indicate the necessity of validation of the markers in various germplasm pools and breeding collections. The SSR markers that are tightly linked to Pl6, Pl8, PlARG would be useful in the sunflower breeding. PlARG homozygous F2 segregants, developed and identified with marker assisted selection in this study, are recommended for further breeding as a new source of genetically determined resistance to downy mildew.

scholarly journals SSR Markers Suitable for Marker Assisted Selection in Sunflower for Downy Mildew Resistance

2018 ◽  
Vol 13 (1) ◽  
pp. 319-326
Author(s):  
Ezgi Çabuk Şahin ◽  
Aral Kalenderoğlu ◽  
Yıldız Aydın ◽  
Göksel Evci ◽  
Ahu Altınkut Uncuoğlu
Keyword(s):  
Downy Mildew ◽  
Resistance Genes ◽  
Marker Assisted Selection ◽  
Downy Mildew Resistance ◽  
Phenotypic Analysis ◽  
Breeding Programs ◽  
Mildew Resistance ◽  
Ssr Analysis ◽  
Genotypic Analysis ◽  
Simple Sequence

AbstractThe effectiveness of Pl genes is known to be resistant to downy mildew (DM) disease affected by fungus Plasmopara halstedii in sunflower. In this study phenotypic analysis was performed using inoculation tests and genotypic analysis were carried out with three DM resistance genes Plarg, Pl13 and Pl8. A total of 69 simple sequence repeat markers and 241 F2 individuals derived from a cross of RHA-419 (R) x P6LC (S), RHA-419 (R) x CL (S), RHA-419 (R) x OL (S), RHA419 (R) x 9758R (S), HA-R5 (R) x P6LC (S) and HA89 (R) x P6LC (S) parental lines were used to identify resistant hybrids in sunflower. Results of SSR analysis using markers linked with downy mildew resistance genes (Plarg, Pl8 and Pl13) and downy mildew inoculation tests were evaluated together and ORS716 (for Plarg and Pl13), HA4011 (for Pl8) markers showed positive correlation with their phenotypic results. These results suggest that these markers are associated with DM resistance and they can be used successfully in marker-assisted selection for sunflower breeding programs specific for downy mildew resistance.

scholarly journals IDENTIFICATION OF POLYMORPHISM ON SIMPLE SEQUENCE REPEATS MARKERS ASSOCIATED WITH BROWN PLANTHOPPER RESISTANCE GENES IN TWENTY RICE GENOTYPES AND THEIR GENETIC RELATIONSHIP

2015 ◽  
Vol 2 (1) ◽  
pp. 98
Author(s):  
Nono Carsono ◽  
Yessikha V. Barus ◽  
Santika Sari ◽  
Winny D. Widarmi ◽  
Danar Dono ◽  
...  
Keyword(s):  
Ssr Markers ◽  
Resistance Genes ◽  
Genetic Relationship ◽  
Polymorphic Information Content ◽  
Durable Resistance ◽  
Brown Planthopper ◽  
Bph Genes ◽  
Rice Genotypes ◽  
Brown Planthopper Resistance ◽  
Simple Sequence

<p>Brown planthopper is one of the most destructive insect pest of rice in Indonesia and other Asian countries. Pyramiding some brown planthopper resistance genes is a valuable approach to create more durable resistance against the pest. The objective of this study was to identify polymorphisms of Brown Planthopper Resistance genes (Bph) on 20 genotypes of rice, and to obtain genetic relationship among genotypes tested. The experiment was conducted from June to September 2012 at Green House and Laboratory of Plant Analysis and Biotechnology, Faculty of Agriculture, Universitas Padjadjaran, Jatinangor. Twenty genotypes were analyzed, and two of them were used as check varieties. Simple Sequence Repeat (SSR) markers were applied to detect Bph3, Bph4, Qbph3, and Qbph4 genes. Polymorphic levels were analyzed by calculating PIC (Polymorphic Information Content). The grouping of rice genotypes were done based on principal components analysis (PCA) of SSR data, and the genetic relationship based on the presence of Bph genes was estimated using UPGMA (Unweighted Pair Group With Arithmetic mean). Results showed that RM313, RM8072, RM8213, RM5953, RM586, and RM589 markers were polymorphic. Rice genotypes PTB 33, Diah Suci, Cibogo, Cisantana, Digul, Ciherang, Inpari 13, Inpari 10, and Memberamo had Bph3, Qbph3, Bph4, and Qbph4. Meanwhile Bph3, Qbph3, and Bph4 were supposed to be belonged by IR 64, Aek Sibundong, Batang Gadis, IR 66, and Mekongga. Kalimas and Tukat Penatu had Bph3, Qbph3, dan Qbph4. IR 74 had Bph3 and Qbph3, and Fatmawati had Bph3 and Bph4. UPGMA clustering resulted in two main clusters, in which the first cluster consisted of 2 subclusters. PTB-33 was closely related with Memberamo, Tukat Penatu, Digul, Diah Suci, and Kalimas. The SSR markers used in this study were proven to be valuable in molecular detection of Bph genes and in estimating genetic relationsips of rice genotypes. PTB-33 was a good donor of resistance genes, as well as Memberamo, Tukat Penatu, Digul, Diah Suci, and Kalimas which were identified as promising donors in rice breeding resistance to brown planthopper. </p><p><strong>Keywords :</strong> Bph gene, Brown Planthopper, Genetic relationship, SSR markers.</p>

scholarly journals PRELIMNARY RESULTS ON MARKER ASSISTED SELECTION FOR DISEASE RESISTANCE IN COMMON WHEAT (TRITICUM AESTIVUM)

1970 ◽  
Vol 62 ◽  
Author(s):  
C. Botez ◽  
Monica Iuoraş ◽  
P. Raica ◽  
N. N. Saulescu
Keyword(s):  
Triticum Aestivum ◽  
Disease Resistance ◽  
Bread Wheat ◽  
Ssr Markers ◽  
Resistance Genes ◽  
Marker Assisted Selection ◽  
Rapd Markers ◽  
Issr Markers ◽  
Resistant Lines ◽  
Selection For

Our attempts to find RAPD markers linked with resistance genes to Septoria and Tilletia relying on co-segregation and to test the SSR markers, identified in literature, in order to select bread wheat resistant to Septoria and Tilletia are presented. Several candidate RAPD markers possibly linked with Septoria resistance genes and fewer linked with Tilletia resistance genes were identified. Some RAPD and SSR markers were specific for some resistant lines. These markers, probably, marked different resistance genes in different lines. It seems that some Septoria resistance genes came from rye genome because these genes were marked with some primers specific for rye genome. We found segregation for these markers in several lines, indicating that the analyzed lines were not completely stabilized. The ISSR markers used could not discriminate the resistant from susceptible genotypes to Septoria and Tilletia.

scholarly journals Novel polymorphic microsatellite markers from turmeric, Curcuma longa L. (Zingiberaceae)

2013 ◽  
Vol 72 (2) ◽  
pp. 407-412 ◽  
Author(s):  
Siju Senan ◽  
Dhanya Kizhakayil ◽  
Thotten E. Sheeja ◽  
Bhaskaran Sasikumar ◽  
Alangar I. Bhat ◽  
...  
Keyword(s):  
Genetic Analysis ◽  
Microsatellite Markers ◽  
Ssr Markers ◽  
Microsatellite Loci ◽  
Simple Sequence Repeat ◽  
Curcuma Longa ◽  
Sequence Repeat ◽  
Discrimination Power ◽  
Polymorphic Microsatellite ◽  
Simple Sequence

Abstract - Twenty one polymorphic microsatellite loci were isolated and characterized from turmeric (Curcuma longa L.). These markers were screened across thirty accessions. The number of alleles observed for each locus ranged from two to eight with an average of 4.7 alleles per locus. The discrimination power of these markers ranged from 0.25 to 0.67 (average 0.6). The simple sequence repeat (SSR) markers can complement the currently available SSR markers and would be useful for the genetic analysis of turmeric accessions.

scholarly journals EVALUATION OF GRAPEVINE RESISTANCE TO DOWNY AND POWDERY MILDEW IN ‘REGENT’ × ‘BOĞAZKERE’ HYBRID POPULATION SEGREGATING FOR RESISTANCE GENES

2019 ◽  
Vol 18 (1) ◽  
pp. 181-188
Author(s):  
Mina Shidfar ◽  
Murat Akkurt ◽  
Arif Atak ◽  
Ali Ergül ◽  
Gökhan Söylemezoğlu
Keyword(s):  
Powdery Mildew ◽  
Downy Mildew ◽  
Resistance Genes ◽  
Marker Assisted Selection ◽  
Plasmopara Viticola ◽  
Hybrid Population ◽  
Breeding Programs ◽  
The World ◽  
Genomic Regions ◽  
New Cultivars

Downy (Plasmopara viticola) and powdery mildew (Erysipha necator) is known as one of the most mischievous diseases for viticulture in Turkey as well as in the world. Therefore breeding studies play an important role for development of new cultivars resistant against fungal diseases. The aim of this research was to develop new F1 population and evaluate the resistance of hybrids to powdery and downy mildew via marker assisted selection (MAS). Resistant ‘Regent’ and susceptible ‘Boğazkere’ varieties were used to obtain hybrid population. A total of 6 markers belonging to 3 genomic regions were used for DNA based selection. Four SSR (UDV15, VMCNG2f12, VMC7F2 and UDV305) and two SCAR (ScORNA7-760 and ScORN3-R) markers which were developed to the resistance loci of Rpv3 and Ren3 were used for DNA based selection. The results were evaluated together with powdery mildew inoculation observations. When inoculation observations and MAS were evaluated, genotypes resistant, tolerant and susceptible to powdery and downy mildew were identified. Especially 16 genotypes identified as resistant to powdery mildew, can be used in future breeding programs.

Polymorphism of PCR-based markers targeting exons, introns, promoter regions, and SSRs in maize and introns and repeat sequences in oat

Genome ◽  
2001 ◽  
Vol 44 (6) ◽  
pp. 1065-1076 ◽  
Author(s):  
J B Holland ◽  
S J Helland ◽  
N Sharopova ◽  
D C Rhyne
Keyword(s):  
Ssr Markers ◽  
Inbred Lines ◽  
Amplification Product ◽  
Promoter Regions ◽  
Repeat Sequences ◽  
Sequence Tagged Sites ◽  
Maize Genotype ◽  
Oat Cultivars ◽  
Ssr Polymorphism ◽  
Simple Sequence

Sequence databases could be efficiently exploited for development of DNA markers if it were known which gene regions reveal the most polymorphism when amplified by PCR. We developed PCR primer pairs that target specific regions of previously sequenced genes from Avena and Zea species. Primers were targeted to amplify 40 introns, 24 exons, and 23 promoter regions within 54 maize genes. We surveyed 48 maize inbred lines (previously assayed for simple-sequence repeat (SSR) polymorphism) for amplification-product polymorphism. We also developed primers to target 14 SSRs and 12 introns within 18 Avena genes, and surveyed 22 hexaploid oat cultivars and 2 diploid Avena species for amplification-product polymorphism. In maize, 67% of promoter markers, 58% of intron markers, and 13% of exon markers exhibited amplification-product polymorphisms. Among polymorphic primer pairs in maize, genotype diversity was highest for SSR markers (0.60) followed by intron markers (0.46), exon markers (0.42), and promoter markers (0.28). Among all Avena genotypes, 64% of SSR markers and 58% of intron markers revealed polymorphisms, but among the cultivars only, 21% of SSR markers and 50% of intron markers were polymorphic. Polymorphic-sequence-tagged sites for plant-breeding applications can be created easily by targeting noncoding gene regions.Key words: Avena, Zea, genetic diversity, DNA sequence.

scholarly journals Analysis of simple sequence repeat (SSR) polymorphism between N22 and Uma rice varieties for marker assisted selection

2018 ◽  
Vol 9 (2) ◽  
pp. 511
Author(s):  
Swapnil Gorakh Waghmare ◽  
P. Sindhumole ◽  
M. R. Shylaja ◽  
Deepu Mathew ◽  
Rose Mary Francies ◽  
...  
Keyword(s):  
Simple Sequence Repeat ◽  
Marker Assisted Selection ◽  
Sequence Repeat ◽  
Rice Varieties ◽  
Ssr Polymorphism ◽  
Simple Sequence

scholarly journals Linkage of RAPD and SSR Markers to Thorniness and Floricane Fruiting in Blackberry

HortScience ◽  
2005 ◽  
Vol 40 (4) ◽  
pp. 1147B-1147
Author(s):  
Eric Stafne ◽  
John Clark ◽  
Kim Lewers
Keyword(s):  
Molecular Markers ◽  
Random Sample ◽  
Ssr Markers ◽  
Simple Sequence Repeats ◽  
Marker Assisted Selection ◽  
Morphological Traits ◽  
Rapd Markers ◽  
Randomly Amplified Polymorphic Dna ◽  
Simple Sequence

Molecular markers have been used previously to identify linkages to important traits of interest. In this study two marker types, randomly amplified polymorphic DNA (RAPD) and simple sequence repeats (SSR), were used to find molecular markers linked to two morphological traits in blackberry (Rubus L. subgenus Rubus). Thorniness and floricane fruiting are both qualitative, recessive traits that are inherited tetrasomically. A cross of `Prime-Jim'® × `Arapaho' was made to create a population that segregated for the two traits. A random sample of 98 plants from a population of 200 were assayed to find molecular markers that co-segregate with the two traits. Three putative markers were identified for the floricane fruiting trait (two SSRs and one RAPD; χ2 = 4.09 to 9.99, P < 0.001 to 0.043). Five potential RAPD markers were found for the thorny trait (χ2 = 3.88 to 10.23, P < 0.001 to 0.048). Identification of markers linked to these traits could potentially be useful in marker-assisted selection.

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