scholarly journals Linkage of RAPD and SSR Markers to Thorniness and Floricane Fruiting in Blackberry

HortScience ◽  
2005 ◽  
Vol 40 (4) ◽  
pp. 1147B-1147
Author(s):  
Eric Stafne ◽  
John Clark ◽  
Kim Lewers
Keyword(s):  
Molecular Markers ◽  
Random Sample ◽  
Ssr Markers ◽  
Simple Sequence Repeats ◽  
Marker Assisted Selection ◽  
Morphological Traits ◽  
Rapd Markers ◽  
Randomly Amplified Polymorphic Dna ◽  
Simple Sequence

Molecular markers have been used previously to identify linkages to important traits of interest. In this study two marker types, randomly amplified polymorphic DNA (RAPD) and simple sequence repeats (SSR), were used to find molecular markers linked to two morphological traits in blackberry (Rubus L. subgenus Rubus). Thorniness and floricane fruiting are both qualitative, recessive traits that are inherited tetrasomically. A cross of `Prime-Jim'® × `Arapaho' was made to create a population that segregated for the two traits. A random sample of 98 plants from a population of 200 were assayed to find molecular markers that co-segregate with the two traits. Three putative markers were identified for the floricane fruiting trait (two SSRs and one RAPD; χ2 = 4.09 to 9.99, P < 0.001 to 0.043). Five potential RAPD markers were found for the thorny trait (χ2 = 3.88 to 10.23, P < 0.001 to 0.048). Identification of markers linked to these traits could potentially be useful in marker-assisted selection.

scholarly journals Application of molecular markers in sunflower breeding

Genetika ◽  
2007 ◽  
Vol 39 (1) ◽  
pp. 1-11 ◽  
Author(s):  
Dejana Saftic-Pankovic
Keyword(s):  
Molecular Markers ◽  
Ssr Markers ◽  
Marker Assisted Selection ◽  
Rapd Markers ◽  
Inbred Lines ◽  
Vegetable Crops ◽  
Large Variability ◽  
Parental Lines ◽  
Early Phases ◽  
Selection Of

The results of the application of molecular markers in sunflower breeding obtained in the Institute of Field and Vegetable Crops in the last decade are reviewed. Our results on genetic distance (GD=7-75%) between sunflower inbred lines obtained with RAPD and SSR markers, indicate large variability and provide important information for the selection of parental lines for future crosses. Interspecific hybridization is often used in sunflower breeding. As only some populations of H. giganteus and H. maximiliani are resistant to sunflower diseases, the investigation of genetic variability in/between two species is of interest. The results obtained with SSR markers are presented. The successful hybridization between H. rigidus and H. annuus was confirmed with RAPD markers, and the variability between F1 and BC1F1 plants is discussed. Desirable alleles and haplotypes can be detected with molecular markers both in early phases of plant development and in early phases of the production of improved lines, which reduces or completely eliminates the large number of testing cycles for desirable phenotypes. CAPS markers for resistance to downy mildew, that can be used in marker assisted selection are presented. .

scholarly journals Genetic Diversity in <i>Jatropha platyphylla</i> Accessions Based on Morphological Traits and Inter-Simple Sequence Repeats Molecular Markers

2021 ◽  
Vol 12 (11) ◽  
pp. 1658-1672
Author(s):  
Edith Salazar-Villa ◽  
Lilia Alcaraz-Meléndez ◽  
Josefina León-Félix ◽  
José Basilio Heredia ◽  
Federico Soto-Landeros ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Molecular Markers ◽  
Simple Sequence Repeats ◽  
Morphological Traits ◽  
Inter Simple Sequence Repeats ◽  
Simple Sequence

scholarly journals Simple Sequence Repeats (SSR) Marker Screening Related to Orange Fleshed Sweet Potato F1 Genotype Resistance against Scab (Sphaceloma batatas Saw.)

2017 ◽  
Vol 2 (6) ◽  
pp. 279
Author(s):  
Nurfitriani Rista ◽  
Fitri Widiantini ◽  
Anna Aina Roosdab ◽  
Endah Yulia ◽  
Agung Karuniawanb
Keyword(s):  
Molecular Markers ◽  
Sweet Potato ◽  
Ssr Markers ◽  
Simple Sequence Repeats ◽  
Pcr Analysis ◽  
Resistant Variety ◽  
Resistant Varieties ◽  
Ssr Primers ◽  
Orange Fleshed Sweet Potato ◽  
Simple Sequence

Orange fleshed sweet potato contains high beta carotene as vitamin A precursor. However, its production is limited by the presence of scab disease caused by Sphaceloma batatas Saw. The disease is able to cause yield loss up to 60%. Best controlling method is using resistant varieties. However, the development of resistant varieties are involving long procedures which is time consuming. The long procedure of resistance varieties selection can be shorted cut using molecular markers such as SSR (Simple Sequence Repeats). Specific SSR markers for sweet potato resistance against scab has not been found. This study aimed to screen SSR molecular markers which were related to resistance to scab. The study used 5 resistant genotypes, 5 susceptible genotypes, and 6 SSR primers. PCR analysis showed that those SSR primers were polymorphic. Furthermore, the biplot analysis result demonstrated that several markers allele were related to plant resistance against scab. This finding indicated that these particular SSR markers can be used in sweet potato breeding program as marker assisted selection to develop resistant variety against scab disease. Keywords: Sphaceloma batatas; orange fleshed sweet potato; SSR markers.

scholarly journals Genotyping of Peach and Nectarine Cultivars with SSR and SRAP Molecular Markers

2004 ◽  
Vol 129 (2) ◽  
pp. 204-210 ◽  
Author(s):  
Riaz Ahmad ◽  
Dan Potter ◽  
Stephen M. Southwick
Keyword(s):  
Molecular Markers ◽  
Ssr Markers ◽  
Simple Sequence Repeat ◽  
Sweet Cherry ◽  
Prunus Persica ◽  
Sequence Repeat ◽  
Srap Markers ◽  
Upgma Cluster Analysis ◽  
Commercially Important ◽  
Simple Sequence

Simple sequence repeat (SSR) and sequence related amplified polymorphism (SRAP) molecular markers were evaluated for detecting intraspecific variation in 38 commercially important peach and nectarine (Prunus persica) cultivars. Out of the 20 SSR primer pairs 17 were previously developed in sweet cherry and three in peach. The number of putative alleles revealed by SSR primer pairs ranged from one to five showing a low level of genetic variability among these cultivars. The average number of alleles per locus was 2.2. About 76% of cherry primers produced amplification products in peach and nectarine, showing a congeneric relationship within Prunus species. Only nine cultivars out of the 38 cultivars could be uniquely identified by the SSR markers. For SRAP, the number of fragments produced was highly variable, ranging from 10 to 33 with an average of 21.8 per primer combination. Ten primer combinations resulted in 49 polymorphic fragments in this closely related set of peaches and nectarines. Thirty out of the 38 peach and nectarine cultivars were identified by unique SRAP fingerprints. UPGMA Cluster analysis based on the SSR and SRAP polymorphic fragments was performed; the relationships inferred are discussed with reference to the pomological characteristics and pedigree of these cultivars. The results indicated that SSR and SRAP markers can be used to distinguish the genetically very close peach and nectarine cultivars as a complement to traditional pomological studies. However, for fingerprinting, SRAP markers appear to be much more effective, quicker and less expensive to develop than are SSR markers.

scholarly journals Development of EST-derived SSR Markers with Long-core Repeat in Olive and Their Use for Paternity Testing

2013 ◽  
Vol 138 (4) ◽  
pp. 290-296 ◽  
Author(s):  
Raúl De la Rosa ◽  
Angjelina Belaj ◽  
Antonio Muñoz-Mérida ◽  
Oswaldo Trelles ◽  
Inmaculada Ortíz-Martín ◽  
...  
Keyword(s):  
Ssr Markers ◽  
Simple Sequence Repeats ◽  
Expressed Sequence Tag ◽  
Paternity Testing ◽  
High Level ◽  
Repeated Motifs ◽  
Expressed Sequence ◽  
Simple Sequence ◽  
Olive Breeding ◽  
Genomic Project

In the present work, a set of eight new hexa-nucleotide simple sequence repeats (SSRs) is reported in olive (Olea europaea L). These SSRs loci were generated on the basis of expressed sequence tag (EST) sequences in the frame of an olive genomic project. The markers showed a high level of polymorphism when tested on a set of cultivars used as genitors in the olive breeding program of Córdoba, Spain. The long-core repeat motif of these markers allows a wider separation among alleles, thus permitting an accurate genotyping. Besides, these markers showed comparable levels of polymorphism to di-nucleotide SSRs, the only ones so far reported in olive. Selected on the basis of their discrimination capacity, four of the eight SSRs were used to test their ability for paternity testing in a total of 81 seedlings coming from 12 crosses. The paternity testing showed that seven crosses matched the alleged paternity and the remaining five were products of illicit pollinations. These results exactly matched with previous paternity testing performed with di-nucleotide SSR markers. These results demonstrate the usefulness of the developed hexa-nucleotide repeated motifs for checking the paternity of breeding progenies and suggest their use on variability studies.

Validation of Microsatellite Markers of Pl Resistance Genes to Downy Mildew of Sunflower

Helia ◽  
2018 ◽  
Vol 41 (68) ◽  
pp. 73-82
Author(s):  
A. Solodenko
Keyword(s):  
Downy Mildew ◽  
Ssr Markers ◽  
Resistance Genes ◽  
Microsatellite Loci ◽  
Marker Assisted Selection ◽  
Parental Line ◽  
Gene Control ◽  
Genetically Determined ◽  
Ssr Polymorphism ◽  
Simple Sequence

AbstractSimple sequence repeats (SSR) polymorphism of 34 microsatellite loci (LG1, 8 and 13) was studied in lines carrying the downy mildew resistance genes Pl and lines with no Pl. The microsatellite loci ORS328 and ORS781 were selected as markers for genes Pl6 and Pl8 in lines HA 335 and QHP-1, respectively. Markers were identified for gene PlARG in RHA 419 and some accessions of H. argophyllus. The SSR markers ORS509, ORS605, ORS610, ORS1182 and ORS1039 were proven to reliably identify the parental line carrying PlARG gene, control and select the heterozygous F1 hybrids and identify homozygous genotypes in F2 generations. Obtained results indicate the necessity of validation of the markers in various germplasm pools and breeding collections. The SSR markers that are tightly linked to Pl6, Pl8, PlARG would be useful in the sunflower breeding. PlARG homozygous F2 segregants, developed and identified with marker assisted selection in this study, are recommended for further breeding as a new source of genetically determined resistance to downy mildew.

scholarly journals ISSR (Inter Simple Sequence Repeats) as molecular markers to study genetic diversity in tarantulas (Araneae, Mygalomorphae)

2009 ◽  
Vol 37 (1) ◽  
pp. 10-14 ◽  
Author(s):  
Salima Machkour‐M'Rabet ◽  
Yann Hénaut ◽  
Ariane Dor ◽  
Gabriela Pérez-Lachaud ◽  
Céline Pélissier ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Molecular Markers ◽  
Simple Sequence Repeats ◽  
Inter Simple Sequence Repeats ◽  
Simple Sequence

Genetic diversity and relationships among Egyptian Galium (Rubiaceae) and related species using ISSR and RAPD markers

Biologia ◽  
2014 ◽  
Vol 69 (3) ◽  
Author(s):  
Kadry Abdel Khalik ◽  
Magdy Abd El-Twab ◽  
Rasha Galal
Keyword(s):  
Genetic Diversity ◽  
Genetic Variation ◽  
Rapd Markers ◽  
Phylogenetic Analyses ◽  
Randomly Amplified Polymorphic Dna ◽  
Inter Simple Sequence Repeats ◽  
Close Relationship ◽  
Rapd And Issr ◽  
Simple Sequence ◽  
Issr Primers

AbstractGenetic diversity and phylogenetic analyses of 24 species, representing nine sections of the genus Galium (Rubiaceae), have been made using the Inter Simple Sequence Repeats (ISSR), Randomly Amplified Polymorphic DNA (RAPD), and combined ISSR and RAPD markers. Four ISSR primers and three RAPD primers generated 250 polymorphic amplified fragments. The results of this study showed that the level of genetic variation in Galium is relatively high. RAPD markers revealed a higher level of polymorphism (158 bands) than ISSR (92 bands). Clustering of genotypes within groups was not similar when RAPD and ISSR derived dendrograms were compared. Six clades can be recognized within Galium, which mostly corroborate, but also partly contradict, traditional groupings. UPGMA-based dendrogram showed a close relationship between members of section Leiogalium with G. verum and G. humifusum (sect. Galium), and G. angustifolium (sect. Lophogalium). Principal coordinated analysis, however, showed some minor differences with UPGMA-based dendrograms. The more apomorphic groups of Galium form the section Leiogalium clade including the perennial sections Galium, Lophogalium, Jubogalium, Hylaea and Leptogalium as well as the annual section Kolgyda. The remaining taxa of Galium are monophyletic.

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