scholarly journals PRELIMNARY RESULTS ON MARKER ASSISTED SELECTION FOR DISEASE RESISTANCE IN COMMON WHEAT (TRITICUM AESTIVUM)

1970 ◽  
Vol 62 ◽  
Author(s):  
C. Botez ◽  
Monica Iuoraş ◽  
P. Raica ◽  
N. N. Saulescu
Keyword(s):  
Triticum Aestivum ◽  
Disease Resistance ◽  
Bread Wheat ◽  
Ssr Markers ◽  
Resistance Genes ◽  
Marker Assisted Selection ◽  
Rapd Markers ◽  
Issr Markers ◽  
Resistant Lines ◽  
Selection For

Our attempts to find RAPD markers linked with resistance genes to Septoria and Tilletia relying on co-segregation and to test the SSR markers, identified in literature, in order to select bread wheat resistant to Septoria and Tilletia are presented. Several candidate RAPD markers possibly linked with Septoria resistance genes and fewer linked with Tilletia resistance genes were identified. Some RAPD and SSR markers were specific for some resistant lines. These markers, probably, marked different resistance genes in different lines. It seems that some Septoria resistance genes came from rye genome because these genes were marked with some primers specific for rye genome. We found segregation for these markers in several lines, indicating that the analyzed lines were not completely stabilized. The ISSR markers used could not discriminate the resistant from susceptible genotypes to Septoria and Tilletia.

scholarly journals The determination of the resistance inheritance against common bunt in wheat and half-diallel hybrids

2019 ◽  
Vol 55 (No. 4) ◽  
pp. 254-260
Author(s):  
Gülçin Akgören Palabiyik ◽  
İsmail Poyraz ◽  
Ahmet Umay
Keyword(s):  
Disease Resistance ◽  
Bread Wheat ◽  
Resistance Genes ◽  
Common Bunt ◽  
Wheat Varieties ◽  
Complete Dominance ◽  
Selection For ◽  
Half Diallel ◽  
Dominant Genes

This study was conducted to determine the inheritance of common bunt resistance in twelve bread wheat varieties and their half-diallel hybrids in Turkey. The disease ratings were performed on the F2 generations of the hybrids in field conditions. The obtained data were analysed by the χ2 test to determine the effective gene numbers and inheritance type in the disease resistance. In addition, the data were evaluated according to the Jinks-Hayman diallel analyses. In conclusion, it was found that of the twelve wheat parents, four contained three resistance genes and four of them contain two resistance genes. The dominant genes were prominent in the population and complete dominance was present. Therefore, the selection for disease resistance should be delayed until the following generations.

scholarly journals 997 USE OF MOLECULAR MARKERS IN BREEDING FOR RESISTANCE TO FUNGAL PATHOGENS

HortScience ◽  
1994 ◽  
Vol 29 (5) ◽  
pp. 572c-572
Author(s):  
James D. Kell
Keyword(s):  
Molecular Markers ◽  
Disease Resistance ◽  
Resistance Genes ◽  
Fungal Pathogens ◽  
Rapd Markers ◽  
Resistance Breeding ◽  
Horticultural Crops ◽  
Allele Specific ◽  
Disease Resistance Breeding ◽  
Selection For

The use of RAPD markers in disease resistance breeding has been successfully demonstrated in horticultural crops. The identification of RAPD markers and their potential role in disease resistance breeding was first demonstrated in tomato; the procedure of bulk segregant analysis for detecting linkages between RAPD markers and genes conditioning resistance to downy mildew was described in lettuce; the use of linked RAPD markers to facilitate the efficient pyramiding of epistatic rust resistance genes to prolong their durability has been illustrated in common bean; the development of allele specific or sequence characterized primers linked to resistance genes has been achieved in pea, bean, tomato, and lettuce Indirect selection for disease resistance based on molecular markers is not a replacement for classical breeding procedures. Used as tools, markers offer breeders unique opportunities to combine resistance to an array of different pathogens and efficiently pyramid epistatic resistance genes for highly variable fungal pathogens. This would not always be possible in the absence of linked markers.

scholarly journals Relatedness severely impacts accuracy of marker-assisted selection for disease resistance in hybrid wheat

Heredity ◽  
2013 ◽  
Vol 112 (5) ◽  
pp. 552-561 ◽  
Author(s):  
M Gowda ◽  
Y Zhao ◽  
T Würschum ◽  
C FH Longin ◽  
T Miedaner ◽  
...  
Keyword(s):  
Disease Resistance ◽  
Marker Assisted Selection ◽  
Hybrid Wheat ◽  
Selection For

Identification of RAPD markers linked to a gene governing cadmium uptake in durum wheat

Genome ◽  
1995 ◽  
Vol 38 (3) ◽  
pp. 543-547 ◽  
Author(s):  
Greg A. Penner ◽  
Leslie J. Bezte ◽  
Dave Leisle ◽  
John Clarke
Keyword(s):  
Durum Wheat ◽  
Gel Electrophoresis ◽  
Marker Assisted Selection ◽  
Rapd Markers ◽  
Dna Analysis ◽  
Random Amplified Polymorphic Dna ◽  
Cadmium Uptake ◽  
Cadmium Content ◽  
Diverse Range ◽  
Selection For

Temperature sweep gel electrophoresis in combination with random amplified polymorphic DNA analysis was employed to detect two markers for a single gene governing low cadmium uptake in western Canadian durum wheat (Triticum turgidum L. var. durum). Analysis of progeny derived from a cross of the high cadmium accumulating cultivar Kyle by the low cadmium accumulating cultivar Nile resulted in linkage estimates of 4.6 (OPC-20) and 21.2 (UBC-180) cM. The closest marker (OPC-20) was shown to be useful for making low cadmium uptake selections from two other sources of low cadmium, 'Biodur' and 'Hercules'. The marker was further used to determine the genetic basis of resistance in 20 introduced durum wheat lines. Within this diverse range of germplasm the marker was correlated with cadmium contents as expected in all but two cases. Plant breeding selection for low cadmium genotypes is hindered by the high cost of chemical determination of cadmium content. Marker assisted selection for a low cadmium uptake gene offers an effective alternative.Key words: cadmium, durum wheat, RAPD markers, marker assisted selection, temperature sweep gel electrophoresis.

Validation of Microsatellite Markers of Pl Resistance Genes to Downy Mildew of Sunflower

Helia ◽  
2018 ◽  
Vol 41 (68) ◽  
pp. 73-82
Author(s):  
A. Solodenko
Keyword(s):  
Downy Mildew ◽  
Ssr Markers ◽  
Resistance Genes ◽  
Microsatellite Loci ◽  
Marker Assisted Selection ◽  
Parental Line ◽  
Gene Control ◽  
Genetically Determined ◽  
Ssr Polymorphism ◽  
Simple Sequence

AbstractSimple sequence repeats (SSR) polymorphism of 34 microsatellite loci (LG1, 8 and 13) was studied in lines carrying the downy mildew resistance genes Pl and lines with no Pl. The microsatellite loci ORS328 and ORS781 were selected as markers for genes Pl6 and Pl8 in lines HA 335 and QHP-1, respectively. Markers were identified for gene PlARG in RHA 419 and some accessions of H. argophyllus. The SSR markers ORS509, ORS605, ORS610, ORS1182 and ORS1039 were proven to reliably identify the parental line carrying PlARG gene, control and select the heterozygous F1 hybrids and identify homozygous genotypes in F2 generations. Obtained results indicate the necessity of validation of the markers in various germplasm pools and breeding collections. The SSR markers that are tightly linked to Pl6, Pl8, PlARG would be useful in the sunflower breeding. PlARG homozygous F2 segregants, developed and identified with marker assisted selection in this study, are recommended for further breeding as a new source of genetically determined resistance to downy mildew.

scholarly journals Marker-assisted selection for rice brown planthopper (Nilaparvata lugens)resistance using linked SSR markers

2015 ◽  
Vol 39 ◽  
pp. 666-673 ◽  
Author(s):  
Mahmoodreza SHABANIMOFRAD ◽  
Mohd Rafii YUSOP ◽  
Sadegh ASHKANI ◽  
Mohamed Hanafi MUSA ◽  
Nur Azura ADAM ◽  
...  
Keyword(s):  
Ssr Markers ◽  
Marker Assisted Selection ◽  
Brown Planthopper ◽  
Nilaparvata Lugens ◽  
Selection For
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