The impact of oxytocin on thiol/disulphide and malonyldialdehyde/glutathione homeostasis in stressed rats

2020 ◽  
Vol 401 (11) ◽  
pp. 1283-1292
Author(s):  
Hilal Korkmaz ◽  
Deniz Önal ◽  
Murat Alışık ◽  
Özcan Erel ◽  
Bilge Pehlivanoğlu
Keyword(s):  
Stress Response ◽  
Acute Stress ◽  
Oxidant Stress ◽  
Corticosterone Level ◽  
Protective Measure ◽  
Serum Samples ◽  
Plus Maze ◽  
Immobilisation Stress ◽  
The Impact ◽  
Antioxidant Effects

AbstractWe aimed to investigate the impact of oxytocin on serum thiol/disulphide and malonylyldialdehyde (MDA)/glutathione balance under acute stress (AS) and chronic stress (CS) exposure in rats. Animals were allocated into control (C), AS and CS groups, then the groups subdivided as intranasal oxytocin or saline applied groups, randomly. Animals in the AS or CS groups were exposed to combined cold-immobilisation stress. Salivary corticosterone levels and elevated plus maze (EPM) scores were used to assess stress response. MDA, glutathione, thiol-disulphide levels were measured in the serum samples. Oxytocin treatment attenuated stress response regardless of the stress duration verified by lower corticosterone level and favorable profile in EPM parameters measured. Furthermore, oxytocin modulated oxidant profile suggesting lowered oxidant stress with decreased serum MDA/glutathione and disulfide/native thiol ratios. Oxytocin improves the response of organism to stress via both its anxiolytic and antioxidant effects. That’s why it can be considered as a protective measure to employ methods to increase endogenous oxytocin and/or to apply exogenous oxytocin to prevent stress-induced increase in oxidant stress, which plays a pivotal role in the pathogenesis of various stress-related diseases.

scholarly journals The association of the 5-HTTLPR polymorphism and the response to different stressors in healthy males

2021 ◽  
Author(s):  
Leandra Kuhn ◽  
Hannes Noack ◽  
Nadine Skoluda ◽  
Lisa Wagels ◽  
Ann-Kristin Röhr ◽  
...  
Keyword(s):  
Working Memory ◽  
Stress Response ◽  
Serotonin Transporter ◽  
Stress Reactivity ◽  
Acute Stress ◽  
Serotonin Transporter Gene ◽  
Transporter Gene ◽  
Subjective Stress ◽  
Stress Induction ◽  
The Impact

AbstractThe experience of stress is related to individual wellbeing and vulnerability to psychopathology. Therefore, understanding the determinants of individual differences in stress reactivity is of great concern from a clinical perspective. The functional promotor polymorphism of the serotonin transporter gene (5-HTTLPR/rs25531) is such a factor, which has been linked to the acute stress response as well as the adverse effect of life stressors. In the present study, we compared the impact of two different stress induction protocols (Maastricht Acute Stress Test and ScanSTRESS) and the respective control conditions on affective ratings, salivary cortisol levels and cognitive performance. To this end, 156 healthy young males were tested and genotyped for the 5-HTTLPR/rs25531 polymorphism. While combined physiological and psychological stress in the MAST led to a greater cortisol increase compared to control conditions as well as the psychosocial ScanSTRESS, subjective stress ratings were highest in the ScanSTRESS condition. Stress induction in general affected working memory capacity but not response inhibition. Subjective stress was also influenced by 5-HTTLPR/rs25531 genotype with the high expression group showing lower stress ratings than lower expression groups. In line with previous research, we identified the low expression variant of the serotonin transporter gene as a risk factor for increased stress reactivity. While some dimensions of the human stress response may be stressor specific, cognitive outcomes such as working memory performance are influenced by stress in general. Different pathways of stress processing and possible underlying mechanisms are discussed.

scholarly journals Loss of CREBRF Reduces Anxiety-like Behaviors and Circulating Glucocorticoids in Male and Female Mice

Endocrinology ◽  
2020 ◽  
Vol 161 (11) ◽  
Author(s):  
Krystle A Frahm ◽  
Akeem A Williams ◽  
Ashlee N Wood ◽  
Michael C Ewing ◽  
Polly E Mattila ◽  
...  
Keyword(s):  
Stress Responses ◽  
Acute Stress ◽  
Plasma Corticosterone ◽  
Affective States ◽  
Male And Female ◽  
Female Mice ◽  
Glucocorticoid Signaling ◽  
Plus Maze ◽  
The Impact

Abstract Glucocorticoid signaling controls many key biological functions ranging from stress responses to affective states. The putative transcriptional coregulator CREB3 regulatory factor (CREBRF) reduces glucocorticoid receptor levels in vitro, suggesting that CREBRF may impact behavioral and physiological outputs. In the present study, we examined adult male and female mice with global loss of CREBRF (CrebrfKO) for anxiety-like behaviors and circulating glucocorticoids in response to various acute stress conditions. Results demonstrate that both male and female CrebrfKO mice have preserved locomotor activity but reduced anxiety-like behaviors during the light–dark box and elevated plus maze. These behavioral phenotypes were associated with lower plasma corticosterone after restraint stress. Further studies using unhandled female mice also demonstrated a loss of the diurnal circulating corticosterone rhythm in CrebrfKO mice. These results suggest that CREBRF impacts anxiety-like behavior and circulating glucocorticoids in response to acute stressors and serves as a basis for future mechanistic studies to define the impact of CREBRF in glucocorticoid-associated behavioral and physiological responses.

scholarly journals The autism-mutated ADNP plays a key role in stress response

2019 ◽  
Vol 9 (1) ◽  
Author(s):  
Shlomo Sragovich ◽  
Yarden Ziv ◽  
Sharon Vaisvaser ◽  
Noam Shomron ◽  
Talma Hendler ◽  
...  
Keyword(s):  
Stress Response ◽  
Elevated Plus Maze ◽  
De Novo ◽  
Social Recognition ◽  
Mammalian Brain ◽  
Plus Maze ◽  
Pituitary Adenylate Cyclase ◽  
Pre Treatment ◽  
The Impact ◽  
Activity Dependent

Abstract Activity-dependent neuroprotective protein (ADNP), discovered and first characterized in our laboratory (IG), is vital for mammalian brain formation and presents one of the leading genes mutated de novo causing an autistic syndrome, namely the ADNP syndrome. Furthermore, a unique mouse model of Adnp-haploinsufficiency was developed in the laboratory (IG), with mice exhibiting cognitive and social deficiencies. ADNP is regulated by vasoactive intestinal peptide (VIP), and pituitary adenylate cyclase-activating peptide (PACAP). In this respect, PACAP was independently identified as a sexual divergent master regulator of the stress response. Here, we sought to determine the impact of the Adnp genotype and the efficacy of PACAP pre-treatment when subjecting Adnp+/− mice to stressful conditions. Significant sex differences were observed with Adnp+/− males being more susceptible to stress in the object and social recognition tests, and the females more susceptible in the open field and elevated plus maze tests. Splenic Adnp expression and plasma cortisol levels in mice were correlated with cognition (male mice) and anxiety-related behavior. These findings were further translated to humans, with observed correlations between ADNP expression and stress/cortisol content in a young men cohort. Altogether, our current results may establish ADNP as a marker of stress response.

scholarly journals The impact of maternal care and blood glucose availability on the cortisol stress response in fasted women

2021 ◽  
Author(s):  
Ulrike U. Bentele ◽  
Maria Meier ◽  
Annika B. E. Benz ◽  
Bernadette F. Denk ◽  
Stephanie J. Dimitroff ◽  
...  
Keyword(s):  
Blood Glucose ◽  
Stress Response ◽  
Maternal Care ◽  
Social Stress ◽  
Acute Stress ◽  
Blood Glucose Concentration ◽  
Stress Test ◽  
Trier Social Stress Test ◽  
The Impact ◽  
Glucose Availability

AbstractIndividuals with a history of low maternal care (MC) frequently present a blunted, yet sometimes also show an increased cortisol stress response. Fasted individuals with low blood glucose levels who are exposed to acute stress typically show an attenuated response pattern in this endocrine marker. Despite well-documented metabolic dysregulations after low MC, a possible interaction of both factors has not been investigated yet. Here, we examined the effects of MC and blood glucose concentration on various aspects of the stress response. Fasted women (N = 122, meanage = 22.12, sdage = 2.56) who experienced either very high, high, or low MC (based on the Parental Bonding Instrument) were randomly assigned to consume grape juice (condition sugar), or water (condition water) prior to being exposed to the Trier-Social-Stress-Test for groups. Salivary cortisol and alpha amylase, blood glucose, and mood ratings were assessed repeatedly. Using multilevel mixed models, we replicated the boosting effect of glucose on the cortisol stress response. While we found neither an effect of MC, nor an interaction between MC and blood glucose availability on the cortisol stress response, we observed an effect of MC on the amylase stress response. We discuss the results in the light of links between various stress/energy systems that possibly mediate health-related MC effects.

The impact of acute stress on the neural processing of food cues in bulimia nervosa: Replication in two samples.

2017 ◽  
Vol 126 (5) ◽  
pp. 540-551 ◽  
Author(s):  
Brittany Collins ◽  
Lauren Breithaupt ◽  
Jennifer E. McDowell ◽  
L. Stephen Miller ◽  
James Thompson ◽  
...  
Keyword(s):  
Bulimia Nervosa ◽  
Acute Stress ◽  
Neural Processing ◽  
Food Cues ◽  
Two Samples ◽  
The Impact

Monitoring the acute stress response and its aftermath using combined fMRI/endocrine measurements

2013 ◽  
Vol 46 (06) ◽  
Author(s):  
I Elbau ◽  
SA Kiem ◽  
A Prosser ◽  
I Eidner ◽  
M Czisch ◽  
...  
Keyword(s):  
Stress Response ◽  
Acute Stress ◽  
Acute Stress Response

scholarly journals Natural Infection of Dairy Cows with Bovine Leukemia Virus Affects Immunoglobulin Levels in Saliva and Serum but Not Milk

Pathogens ◽  
2021 ◽  
Vol 10 (7) ◽  
pp. 907
Author(s):  
Monika Dziuba ◽  
Vickie J. Ruggiero ◽  
Catherine Wilson ◽  
Paul C. Bartlett ◽  
Paul M. Coussens
Keyword(s):  
Pilot Study ◽  
Dairy Cows ◽  
Bovine Leukemia Virus ◽  
Leukemia Virus ◽  
Natural Infection ◽  
Total Serum ◽  
Serum Samples ◽  
Retroviral Infection ◽  
Serum Iga ◽  
The Impact

Bovine leukemia virus (BLV) is a retroviral infection that disrupts the immune function of infected animals. It is widespread among U.S. dairy cattle. In this pilot study, the average total IgA and IgM concentrations in milk, saliva, and serum samples from BLV ELISA-positive (ELISA+) dairy cows were compared against samples from BLV ELISA-negative (ELISA−) cows using the Kruskal–Wallis test (with ties). The results from ELISA+ cows were also stratified by lymphocyte count (LC) and proviral load (PVL). In milk and saliva from ELISA+ cows, the average total IgA and IgM concentrations were decreased compared to ELISA− cows, although this was only statistically significant for saliva IgM in cows with low PVL (p = 0.0424). Numerically, the average total IgA concentrations were 33.6% lower in milk and 23.7% lower in saliva, and the average total IgM concentrations were 42.4% lower in milk and 15.5% lower in saliva. No significant differences were observed in the total serum IgA concentrations, regardless of PVL and LC. The total serum IgM from ELISA+ cows was significantly decreased (p = 0.0223), with the largest decreases occurring in the highest PVL and LC subgroups. This pilot study is a first step in investigating the impact of BLV on mucosal immunity and will require further exploration in each of the various stages of disease progression.

Sign in / Sign up

Export Citation Format

Share Document