Influence of extracellular media’s ionic strength on the osmotic stability of Sahel goat erythrocytes

2015 ◽  
Vol 26 (2) ◽  
Author(s):  
Nanacha Afifi Igbokwe ◽  
Ikechukwu Onyebuchi Igbokwe
Keyword(s):  
Ionic Strength ◽  
Osmotic Fragility ◽  
Ionic Concentration ◽  
Fragility Curve ◽  
Osmotic Resistance ◽  
Volume Changes ◽  
Erythrocyte Osmotic Fragility ◽  
Osmotic Stability ◽  
Osmotic Lysis ◽  
Sahel Goat

AbstractHeparinised blood was exposed to osmotic lysis in hypotonic buffered saline to evaluate erythrocyte membrane stability. When KThe erythrocyte osmotic fragility curve in saline was hyperbolic even when the ionic concentration was reduced by 50% with saccharides. Haemolysis was higher with EDTA than heparinised blood at saline concentrations of 90 and 150–180 mosmol/L. The fragility curve was sigmoidal and shifted to the left when saline was completely substituted with a saccharide. The non-ionic saccharides increased erythrocyte osmotic resistance linearly (r=0.88; p<0.02) from median to minimal hyposmolarities (150–300 mosmol/L) and reduced the osmolyte concentration at median fragility by 36%. No effect occurred at <30–120 mosmol/L and >90% fragility; and saccharide concentrations were almost non-lytic at comparable saline concentrations evoking <10% haemolysis. Fragilities were neither affected by period (30–60 min) of incubation nor the type of saccharide used.In this study, the variation in osmotic stability of caprine erythrocytes was linked to ionic strength of the suspending extracellular media which seemed to exert an influence through transmembrane ion fluxes and regulatory volume changes in erythrocytes.

Phenotypic homogeneity with minor deviance in osmotic fragility of Sahel goat erythrocytes in non-ionic sucrose media during various physiologic states

2016 ◽  
Vol 27 (6) ◽  
Author(s):  
Nanacha Afifi Igbokwe ◽  
Ikechukwu Onyebuchi Igbokwe
Keyword(s):  
Osmotic Fragility ◽  
Late Pregnancy ◽  
Erythrocyte Osmotic Fragility ◽  
Osmotic Water ◽  
Sigmoidal Curve ◽  
Osmotic Lysis ◽  
Osmotic Behaviour ◽  
The Mean ◽  
A Minor ◽  
Sahel Goat

AbstractBackground:Erythrocyte swelling in non-ionic sucrose media and the subsequent osmotic lysis are influenced by mechanisms of regulatory volume adjustment and osmotic water permeability. Kinetics of transmembrane water and ion fluxes in varied physiologic states may determine the phenotype of erythrocyte osmotic fragility (EOF) and affect estimates of EOF.Methods:Effects of sex, age, late pregnancy (third trimester) and lactation on the haemolysis of Sahel goat erythrocytes incubated in a series of hyposmotic non-ionic sucrose media were investigated.Results:The fragiligram was sigmoidal in 72 (97%) out of 74 goats. Two male (3%) goats with low and high extreme median erythrocyte fragilities (MEF), had non-sigmoidal curves. The mean fragilities at osmolarities of 30–300 mosmol/L of sucrose and the mean osmolarities responsible for 10%–90% haemolysis (CHConclusions:Physiologic states of the goat did not affect EOF phenotype in non-ionic sucrose media. Sigmoidal fragility phenotype seemed to be homogeneously conserved by osmoregulatory mechanisms not partitioned by sex, age, late pregnancy or lactation, but a minor non-sigmoidal curve might have occurred due to altered erythrocyte osmotic behaviour that would require further investigation.

scholarly journals Diminished osmotic fragility of human erythrocytes following the membrane insertion of oxygenated sterol compounds

Blood ◽  
1981 ◽  
Vol 58 (2) ◽  
pp. 317-325
Author(s):  
RA Streuli ◽  
JR Kanofsky ◽  
RB Gunn ◽  
S Yachnin
Keyword(s):  
Cell Membrane ◽  
Hereditary Spherocytosis ◽  
Osmotic Fragility ◽  
Human Erythrocytes ◽  
Fragility Curve ◽  
Membrane Insertion ◽  
Red Cell ◽  
Red Cell Membrane ◽  
Mean Cell Volume ◽  
Osmotic Lysis

Oxygenated sterol compounds (OSC), when incubated for 1 hr with human erythrocytes in lipoprotein-depleted medium at concentrations of 0.625- 5 X 10(-5) M, are inserted into the cell membrane and remain there despite subsequent washing of the cells. The insertion results in expansion of the surface area of the red cell ghost membrane, an increase in critical hemolytic volume, and as a consequence, in diminished osmotic fragility of the erythrocytes. This effect is seen with echinocyte-forming as well as with non-echinocyte-forming OSC. Erythrocytes treated with OSC do not differ from control cells with respect to their mean cell volume (MCV) in isotonic solution, water content, ion fluxes, and filterability through polycarbonate filters. The shift of the osmotic fragility curve toward lower NaCl concentrations is proportional to the amount of OSC inserted into the red cell membrane. 7 beta-Hydroxycholesterol, 22-ketocholesterol, and 20 alpha-hydroxycholesterol are the most potent inhibitors of osmotic lysis. The effect of OSC on osmotic fragility is diminished if the erythrocytes are incubated in a lipoprotein-containing medium; free cholesterol, however, does not change this effect. Various progesterones also protect red cell from osmotic lysis, but only if the erythrocytes are directly exposed to the compounds present in the hypotonic NaCl solutions used for measurement of their osmotic fragility. Progesterones do not remain in the membrane after the cells have been washed. The OSC are also capable of correcting the osmotic fragility curve of red cells from patients with hereditary spherocytosis. These experiments may suggest an approach to the pharmacologic treatment of hereditary spherocytosis.

scholarly journals Diminished osmotic fragility of human erythrocytes following the membrane insertion of oxygenated sterol compounds

Blood ◽  
1981 ◽  
Vol 58 (2) ◽  
pp. 317-325 ◽  
Author(s):  
RA Streuli ◽  
JR Kanofsky ◽  
RB Gunn ◽  
S Yachnin
Keyword(s):  
Cell Membrane ◽  
Hereditary Spherocytosis ◽  
Osmotic Fragility ◽  
Human Erythrocytes ◽  
Fragility Curve ◽  
Membrane Insertion ◽  
Red Cell ◽  
Red Cell Membrane ◽  
Mean Cell Volume ◽  
Osmotic Lysis

Abstract Oxygenated sterol compounds (OSC), when incubated for 1 hr with human erythrocytes in lipoprotein-depleted medium at concentrations of 0.625- 5 X 10(-5) M, are inserted into the cell membrane and remain there despite subsequent washing of the cells. The insertion results in expansion of the surface area of the red cell ghost membrane, an increase in critical hemolytic volume, and as a consequence, in diminished osmotic fragility of the erythrocytes. This effect is seen with echinocyte-forming as well as with non-echinocyte-forming OSC. Erythrocytes treated with OSC do not differ from control cells with respect to their mean cell volume (MCV) in isotonic solution, water content, ion fluxes, and filterability through polycarbonate filters. The shift of the osmotic fragility curve toward lower NaCl concentrations is proportional to the amount of OSC inserted into the red cell membrane. 7 beta-Hydroxycholesterol, 22-ketocholesterol, and 20 alpha-hydroxycholesterol are the most potent inhibitors of osmotic lysis. The effect of OSC on osmotic fragility is diminished if the erythrocytes are incubated in a lipoprotein-containing medium; free cholesterol, however, does not change this effect. Various progesterones also protect red cell from osmotic lysis, but only if the erythrocytes are directly exposed to the compounds present in the hypotonic NaCl solutions used for measurement of their osmotic fragility. Progesterones do not remain in the membrane after the cells have been washed. The OSC are also capable of correcting the osmotic fragility curve of red cells from patients with hereditary spherocytosis. These experiments may suggest an approach to the pharmacologic treatment of hereditary spherocytosis.

Phenotypic variations in osmotic lysis of Sahel goat erythrocytes in non-ionic glucose media

2016 ◽  
Vol 27 (2) ◽  
Author(s):  
Nanacha Afifi Igbokwe ◽  
Ikechukwu Onyebuchi Igbokwe
Keyword(s):  
Glucose Transporter ◽  
Osmotic Fragility ◽  
Late Pregnancy ◽  
Transporter Protein ◽  
Extracellular Glucose ◽  
Pregnancy And Lactation ◽  
Osmotic Lysis ◽  
Sahel Goat ◽  
In Vitro Interaction

AbstractErythrocyte osmotic lysis in deionised glucose media is regulated by glucose influx, cation efflux, and changes in cell volume after water diffusion. Transmembrane fluxes may be affected by varied expression of glucose transporter protein and susceptibility of membrane proteins to glucose-induced glycosylation and oxidation in various physiologic states.Variations in haemolysis of Sahel goat erythrocytes after incubation in hyposmotic non-ionic glucose media, associated with sex, age, late pregnancy, and lactation, were investigated.The osmotic fragility curve in glucose media was sigmoidal with erythrocytes from goats in late pregnancy (PRE) or lactation (LAC) or from kid (KGT) or middle-aged (MGT) goats. Non-sigmoidal phenotype occurred in yearlings (YGT) and old (OGT) goats. The composite fragility phenotype for males and non-pregnant dry (NPD) females was non-sigmoidal. Erythrocytes with non-sigmoidal curves were more stable than those with sigmoidal curves because of inflectional shift of the curve to the left. Erythrocytes tended to be more fragile with male than female sex, KGT and MGT than YGT and OGT, and LAC and PRE than NPD. Thus, sex, age, pregnancy, and lactation affected the haemolytic pattern of goat erythrocytes in glucose media.The physiologic state of the goat affected the in vitro interaction of glucose with erythrocytes, causing variations in osmotic stability with variants of fragility phenotype. Variations in the effect of high extracellular glucose concentrations on the functions of membrane-associated glucose transporter, aquaporins, and the cation cotransporter were presumed to be relevant in regulating the physical properties of goat erythrocytes under osmotic stress.

scholarly journals Minor Changes in Erythrocyte Osmotic Fragility in Trace Amine-Associated Receptor 5 (TAAR5) Knockout Mice

2021 ◽  
Vol 22 (14) ◽  
pp. 7307
Author(s):  
Ilya S. Zhukov ◽  
Larisa G. Kubarskaya ◽  
Inessa V. Karpova ◽  
Anastasia N. Vaganova ◽  
Marina N. Karpenko ◽  
...  
Keyword(s):  
Knockout Mice ◽  
Emotional Regulation ◽  
Complete Blood Count ◽  
Gene Knockout ◽  
Hematological Parameters ◽  
Osmotic Fragility ◽  
Erythrocyte Osmotic Fragility ◽  
Trace Amine ◽  
Socially Relevant ◽  
The Impact

Trace amine-associated receptors (TAARs) are a group of G protein-coupled receptors that are expressed in the olfactory epithelium, central nervous system, and periphery. TAAR family generally consists of nine types of receptors (TAAR1-9), which can detect biogenic amines. During the last 5 years, the TAAR5 receptor became one of the most intriguing receptors in this subfamily. Recent studies revealed that TAAR5 is involved not only in sensing socially relevant odors but also in the regulation of dopamine and serotonin transmission, emotional regulation, and adult neurogenesis by providing significant input from the olfactory system to the limbic brain areas. Such results indicate that future antagonistic TAAR5-based therapies may have high pharmacological potential in the field of neuropsychiatric disorders. TAAR5 is known to be expressed in leucocytes as well. To evaluate potential hematological side effects of such future treatments we analyzed several hematological parameters in mice lacking TAAR5. In these mutants, we observed minor but significant changes in the osmotic fragility test of erythrocytes and hematocrit levels. At the same time, analysis of other parameters including complete blood count and reticulocyte levels showed no significant alterations in TAAR5 knockout mice. Thus, TAAR5 gene knockout leads to minor negative changes in the erythropoiesis or eryptosis processes, and further research in that field is needed. The impact of TAAR5 deficiency on other hematological parameters seems minimal. Such negative, albeit minor, effects of TAAR5 deficiency should be taken into account during future TAAR5-based therapy development.

Effects of melatonin on cloacal temperature and erythrocyte osmotic fragility in layer hens during the hot-dry season

2014 ◽  
Vol 43 (1) ◽  
pp. 52-60 ◽  
Author(s):  
Victor Olusegun Sinkalu ◽  
Joseph Olusegun Ayo ◽  
Ariyo Adelaja Abimbola ◽  
Josiah Egbamushe Ibrahim
Keyword(s):  
Osmotic Fragility ◽  
Dry Season ◽  
Erythrocyte Osmotic Fragility ◽  
Hot Dry Season ◽  
Layer Hens

Erythrocyte osmotic fragility in response to a short road transport in cattle, horses, and goats

2016 ◽  
Vol 12 ◽  
pp. 82-84 ◽  
Author(s):  
Francesco Fazio ◽  
Stefania Casella ◽  
Claudia Giannetto ◽  
Elisabetta Giudice ◽  
Giuseppe Piccione
Keyword(s):  
Osmotic Fragility ◽  
Road Transport ◽  
Erythrocyte Osmotic Fragility

scholarly journals Seasonal Variation in the Haematological Parameters of the Adult Mallard Duck in a Tropical Environment

2020 ◽  
pp. 30-37
Author(s):  
O. S. Olawuwo ◽  
O. I. Azeez ◽  
J. O. Oyewale
Keyword(s):  
Seasonal Variation ◽  
Osmotic Fragility ◽  
Dry Season ◽  
Tropical Environment ◽  
Haematological Parameters ◽  
Mallard Duck ◽  
Corpuscular Haemoglobin ◽  
Erythrocyte Osmotic Fragility ◽  
Mean Corpuscular Haemoglobin ◽  
Erythrocyte Fragility

Duck production is a growing poultry enterprise in Nigeria and they are mostly reared in extensive management system. However, the haematological profiles as influenced by the tropical environment have not been well documented. The objective of the present study was to examine the seasonal variation in the haematological parameters of the adult Mallard duck in the tropical environment of Nigeria; as they effects duck production adversely. The Erythrocyte, leucocyte and platelet counts, as well as the erythrocyte osmotic fragility of the domestic duck of the mallard breed during the wet and dry seasons in the hot humid tropical environment of the Experimental Animal Unit of the Department of Veterinary Physiology, Biochemistry and Pharmacology, University of Ibadan, Ibadan, Nigeria was investigated. The study showed that the values of the packed cell volume (PCV), mean corpuscular haemoglobin (MCH) and platelet were significantly higher in the dry season than in the wet season, but the red blood cell (RBC), haemoglobin (Hb), mean corpuscular volume (MCV), mean corpuscular haemoglobin concentration (MCHC), total and differential leucocyte values were similar in the two seasons. The erythrocyte fragility was also higher in the dry season. In conclusion the higher PCV, MCH and platelet values in the dry season might have resulted from haemoconcentration occasioned by higher evaporative heat loss, which is a common occurrence in the dry season. The higher erythrocyte fragility could have been the result of stress induced by the high ambient temperature during the dry season, or higher metabolic rate associated with lactic acid accumulation, which has been shown to increase erythrocyte osmotic fragility.

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