Red blood cell morphology in patients with β-thalassemia minor

AbstractBackground:A systematic analysis of the occurrence of red blood cell (RBC) abnormalities in β-thalassemia minor has not been performed to date. This study aimed to identify and quantify the frequency of RBC abnormalities in patients with β-thalassemia minor.Methods:We examined blood smears of 33 patients with β-thalassemia minor by light microscopy for the occurrence of 15 defined RBC abnormalities. In the case of positivity, the abnormal cells/20 high power fields (HPF) at 1000-fold magnification were counted.Results:Anisocytosis, poikilocytosis and target cells (median 42/20 HPF) were observed in all, and ovalocytes in 32 (96.9%, median 10/20 HPF) subjects. Dacryocytes (81.8%), stomatocytes (81.8%, median 10/20 HPF), elliptocytes (75.8%), cells with basophilic stippling (72.7%) and irregularly contracted cells (63.6%) were frequently, and schistocytes (15.2%), bite cells (6%) and pincer cells (3%) were occasionally found.Conclusions:Morphological abnormalities of erythrocytes are common in peripheral blood (PB) smears of patients with β-thalassemia minor. In this study, anisocytosis, poikilocytosis and target cells were apparent in all, and ovalocytes, elliptocytes, cells with basophilic stippling, dacryocytes, stomatocytes and irregularly contracted cells were observed in the majority of the analyzed slides. Our observations may be useful to improve the differential diagnosis of anemia in clinical laboratory routine.

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Detection of Intracellular Parasites by Use of the CellaVision DM96 Analyzer during Routine Screening of Peripheral Blood Smears

Journal of Clinical Microbiology ◽

10.1128/jcm.01783-14 ◽

2014 ◽

Vol 53 (1) ◽

pp. 167-171 ◽

Cited By ~ 15

Author(s):

Lori D. Racsa ◽

Rita M. Gander ◽

Paul M. Southern ◽

Erin McElvania TeKippe ◽

Christopher Doern ◽

...

Keyword(s):

Blood Cell ◽

Cell Morphology ◽

Red Blood Cell ◽

Peripheral Blood ◽

Detection Rate ◽

Detection Rates ◽

Content Type ◽

Blood Smears ◽

Blood Cell Morphology ◽

Peripheral Blood Smears

Conventional microscopy is the gold standard for malaria diagnosis. The CellaVision DM96 is a digital hematology analyzer that utilizes neural networks to locate, digitize, and preclassify leukocytes and characterize red blood cell morphology. This study compared the detection rates ofPlasmodiumandBabesiaspecies on peripheral blood smears utilizing the CellaVision DM96 with the rates for a routine red blood cell morphology scan. A total of 281 slides were analyzed, consisting of 130 slides positive forPlasmodiumorBabesiaspecies and 151 negative controls. Slides were blinded, randomized, and analyzed by CellaVision and microscopy for red cell morphology scans. The technologists were blinded to prior identification results. The parasite detection rate was 73% (95/130) for CellaVision and 81% (105/130) for microscopy for positive samples. The interobserver agreement between CellaVision and microscopy was fair, as Cohen's kappa coefficient equaled 0.36. Pathologist review of CellaVision images identified an additional 15 slides with parasites, bringing the total number of detectable positive slides to 110 of 130 (85%).Plasmodium ovalehad the lowest rate of detection at 56% (5 of 9);Plasmodium malariaeandBabesiaspp. had the highest rate of detection at 100% (3/3 and 6/6, respectively). The detection rate by CellaVision was 100% (23/23) when the parasitemia was ≥2.5%. The detection rate for <0.1% parasitemia was 63% (15/24). Technologists appropriately classified all negative specimens. The percentage of positive specimens detectable by CellaVision (73%) approaches results for microscopy on routine scan of peripheral blood smears for red blood cell morphology.

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Classification of Red Blood Cell Morphology Using Image Processing and Support Vector Machine

Proceedings of the 2020 10th International Conference on Biomedical Engineering and Technology ◽

10.1145/3397391.3397407 ◽

2020 ◽

Author(s):

Paul Daniel C. Divina ◽

John Philip T. Felices ◽

Carlos C. Hortinela ◽

Janette C. Fausto ◽

Flordeliza L. Valiente ◽

...

Keyword(s):

Image Processing ◽

Support Vector Machine ◽

Blood Cell ◽

Cell Morphology ◽

Red Blood Cell ◽

Support Vector ◽

Blood Cell Morphology

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Iron alters red blood cell morphology

Blood ◽

10.1182/blood-2012-09-454793 ◽

2013 ◽

Vol 121 (1) ◽

pp. 9-9 ◽

Cited By ~ 17

Author(s):

Etheresia Pretorius ◽

Boguslaw Lipinski

Keyword(s):

Blood Cell ◽

Cell Morphology ◽

Red Blood Cell ◽

Blood Cell Morphology

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Coarse-grained area-difference-elasticity membrane model coupled with IB–LB method for simulation of red blood cell morphology

Physica A Statistical Mechanics and its Applications ◽

10.1016/j.physa.2018.06.050 ◽

2018 ◽

Vol 509 ◽

pp. 1183-1194 ◽

Cited By ~ 2

Author(s):

Zi-Xiang Tong ◽

Xi Chen ◽

Ya-Ling He ◽

Xiang-Biao Liao

Keyword(s):

Blood Cell ◽

Cell Morphology ◽

Red Blood Cell ◽

Membrane Model ◽

Coarse Grained ◽

Blood Cell Morphology ◽

Area Difference

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Prevention of Thrombocytopenia by Thrombopoietin in Myelosuppressed Rhesus Monkeys Accompanied by Prominent Erythropoietic Stimulation and Iron Depletion

Blood ◽

10.1182/blood.v90.1.58 ◽

1997 ◽

Vol 90 (1) ◽

pp. 58-63 ◽

Cited By ~ 36

Author(s):

Karen J. Neelis ◽

Luo Qingliang ◽

G. Roger Thomas ◽

Bob L. Cohen ◽

Dan L. Eaton ◽

...

Keyword(s):

Blood Cell ◽

Red Blood Cell ◽

Rhesus Monkeys ◽

Normal Values ◽

Cell Lineage ◽

Controlled Study ◽

Total Serum ◽

Target Cells ◽

X Rays ◽

Iron Depletion

Abstract The effectiveness of thrombopoietin (TPO) in alleviating thrombocytopenia was evaluated in a placebo-controlled study involving rhesus monkeys exposed to 5 Gy total-body irradiation (TBI) (300-kV x-rays) to result in 3 weeks of pancytopenia. Supraoptimal treatment with human recombinant TPO (10 μg/kg/d subcutaneously, days 1 to 21 after TBI) was highly effective in preventing thrombocytopenia, with nadirs for thrombocytes, on average, far higher than 100 × 109/L, a greatly accelerated recovery to normal values, and no need for thrombocyte transfusions. TPO appeared to act selectively in that neutrophil regeneration was not influenced but red blood cell lineage recovery was prominently stimulated, with reticulocyte regeneration being initiated 10 days earlier than in placebo-treated animals. The reticulocytosis was followed by a normoblastosis that occurred earlier and was more pronounced than in placebo-treated monkeys. The effect of TPO on the red blood cell lineage was also reflected in a less profound nadir for hemoglobin (Hb) and hematocrit values than in placebo controls. However, this effect was not followed by a rapid recovery to normal values, due to development of a microcytic hypochromic anemia. Iron depletion was demonstrated by measurements of total serum iron and total iron-binding capacity (TIBC) and could be prevented by prophylactic intramuscular (IM) iron before TBI or corrected by IM iron after TPO treatment. Rechallenging with TPO in week 8 after TBI demonstrated a homogenous thrombocyte response similar in magnitude to the initial response, but a greatly diminished reticulocyte response. This demonstrated that the erythropoietic response to TPO administration depends on the hemopoietic state of the animal and may reflect multiple TPO target cells. It is postulated that the extremely rapid erythropoiesis due to TPO treatment in the initial regeneration phase following myelosuppression results in iron depletion by a mechanism similar to that seen following erythropoietin treatment in patients with end-stage renal failure. It is concluded that protracted TPO therapy to counteract thrombocytopenic states may result in iron depletion and that the iron status should be monitored before, during, and after TPO treatment.

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