Hubungan Jumlah Eritrosit dan Hitung Jenis Leukosit pada Masyarakat dengan Paparan Polutan Tinggi di Desa Tambak Mulyo Kota Semarang

Lead (Pb) and carbon monoxide (CO) are gas components in airborne pollutants due to vehicle fumes or industrial exhaust gases that affect the blood, namely shortening the life span of erythrocytes produced in erythropoiesis. In addition, small pollutants can be inhaled and enter the alveoli and then phagocytosis and trigger the release of mediators, namely leukocyte cells, which can cause inflammation. This study aims to determine the correlation between the erythrocytes count and differential leucocyte count of the Tambak Mulyo village community, Semarang City. This research was a cross-sectional analytic study of primary data using 20 blood samples, carried out by the erythrocytes count and differential leucocyte count tests. The examination results showed that the average erythrocytes count in all samples was 5.25 million cells/mm3 with a percentage of the normal erythrocytes count is 45.00%, and a high of erythrocytes count is 55.0%. On the test of the differential leucocyte count, 4 samples are obtained with specific values. Normal leukocytes and as many as 16 samples experienced an increase and decrease in the differential leucocyte count. The chi-square test shows Asymp.Sig> 0.05, which means there is no correlation between variables, so it can be concluded that there is no significant correlation between the erythrocytes count and differential leucocyte count (lymphocytes, basophils, eosinophils, neutrophils, and monocytes) in the community with exposure to high pollutants in Tambak Mulyo village, Semarang City.

Prevalence and intensity of Trypanosoma sp. in wild swamp eels (Synbranchus bengalensis) marketed in Surabaya, Indonesia

Mahasri G, Koesdarto S, Kismiyati, Sari DPW, Santamurti MB, Kandi IW, Fitri SDS, Amin M. 2019. Prevalence and intensity of Trypanosoma sp. in wild swamp eels (Synbranchus bengalensis) marketed in Surabaya, Indonesia. Biodiversitas 20: 3262-3268. Trypanosoma sp. is parasitic protozoa, which can infect not only aquatic organisms but also humans. As the parasite considered a zoonosis disease, there has been a lot of concern about the presence of this parasite in aquaculture commodities. This research aimed to detect and determine the prevalence and intensity of Trypanosoma sp. infection in wild-caught swamp eels (Synbranchus bengalensis) marketed in Surabaya. A total of sixty swamp eels with 47.30±4.69 cm in length were collected from two different locations, Ambengan and Karah, Surabaya, East Java, Indonesia. The swamp eels were transported alive in two aerated plastic bags to the laboratory. The observed parameters were prevalence and intensity of Trypanosoma sp. in the eels’ blood, total erythrocyte counts and total differential leucocyte count (monocytes, lymphocytes, basophils, eosinophil, and neutrophil). The result showed that 7 of 30 (23%) wild-caught swam eels obtained from Ambengan and 9 of 30 eels (30%) collected from Karah were infected by Trypanosoma sp. The intensity of the parasite in eels collected from both locations was considered as moderate, 12.6 parasites/eel (Ambengan) and 5.9 parasites/eel (Karah). Additionally, hematology analysis indicated total erythrocytes count of blood in the infected eels from both locations were significantly lower than total erythrocytes of blood in the non-infected eels, p<0.05. Furthermore, hematology analysis indicated that the numbers of monocyte, lymphocyte, and neutrophil were significantly different than that of non-infected eels, P<0.05. Meanwhile, there was no significant difference in cell counts of basophils and eosinophil in the blood of infected and non-infected eels, p>0,05. These results demonstrate that swamp eels marketed in Surabaya were infected by Trypanosoma sp.