scholarly journals Metabolic activity of sulfate-reducing bacteria from rodents with colitis

Open Medicine ◽  
2018 ◽  
Vol 13 (1) ◽  
pp. 344-349 ◽  
Author(s):  
Jozef Kováč ◽  
Monika Vítězová ◽  
Ivan Kushkevych
Keyword(s):  
Ulcerative Colitis ◽  
Large Intestine ◽  
Sulfate Reducing Bacteria ◽  
Dissimilatory Sulfate Reduction ◽  
Healthy Human ◽  
Sulfate Reducing ◽  
Anaerobic Microorganisms ◽  
The Difference ◽  
High Concentrations ◽  
Reducing Bacteria

AbstractSulfate-reducing bacteria (SRB) are anaerobic microorganisms, which use sulfate as an electron acceptor in the process of dissimilatory sulfate reduction. The final metabolic product of these anaerobic microorganisms is hydrogen sulfide, which is known as toxic and can lead to damage to epithelial cells of the large intestine at high concentrations. Different genera of SRB are detected in the large intestine of healthy human and animals, and with diseases like Crohn’s disease and ulcerative colitis. SRB isolated from rodents with ulcerative colitis have produced 1.14 (mice) and 1.03 (rats) times more sulfide ions than healthy rodents. The species ofDesulfovibriogenus are the most widespread among all SRB in the intestine. The object of our research was to observe and compare the difference of production of sulfide and reduction of sulfate in intestinal SRB isolated from healthy rodents and rodents with ulcerative colitis.

scholarly journals Analysis of pH dose-dependent growth of sulfate-reducing bacteria

Open Medicine ◽  
2019 ◽  
Vol 14 (1) ◽  
pp. 66-74 ◽  
Author(s):  
Ivan Kushkevych ◽  
Dani Dordević ◽  
Monika Vítězová
Keyword(s):  
Ulcerative Colitis ◽  
Hydrogen Sulfide ◽  
Sulfate Reduction ◽  
Sulfate Reducing Bacteria ◽  
Alkaline Media ◽  
Dissimilatory Sulfate Reduction ◽  
Lactate Oxidation ◽  
Sulfate Reducing ◽  
Inflammatory Bowel ◽  
Reducing Bacteria

AbstractLower intraluminal colonic pH is an indication for the development of inflammatory bowel disease including active ulcerative colitis. Involvement of intestinal sulfate-reducing bacteria in decreasing bowel pH by the production of H2S and acetate as well as their sensitivity has never been reported before. The study of the relative pH and survival of Desulfovibrio piger Vib-7 by monitoring sulfate reduction parameters was the aim of this work. Monitoring was done through the measurement of bacterial growth (biomass), dissimilatory sulfate reduction parameters: sulfate consumption, lactate oxidation, hydrogen sulfide and acetate production. According to our results, we observed that lower pH (<5) significantly inhibited D. piger Vib-7 growth. This inhibition was also noticed when alkaline media (>9 pH) was used, though the reduction was not at the rate as in media with pH of 4. The research indicates that the growth of D. piger Vib-7 is inhibited at pH of 4 which is not as low as the pH found in people with severely developed inflammatory bowel diseases such as ulcerative colitis. Certainly the interaction (synergistic effect) between both hydrogen sulfide and acetate accumulation can also play an important etiological role in the development of bowel inflammation in humans and animals.

scholarly journals Analysis of physiological parameters of Desulfovibrio strains from individuals with colitis

2019 ◽  
Vol 13 (1) ◽  
pp. 481-488 ◽  
Author(s):  
Ivan Kushkevych ◽  
Dani Dordević ◽  
Peter Kollár
Keyword(s):  
Ulcerative Colitis ◽  
Bowel Disease ◽  
Principal Component ◽  
Biomass Accumulation ◽  
Sulfate Reducing Bacteria ◽  
Dissimilatory Sulfate Reduction ◽  
Lactate Oxidation ◽  
Sulfate Reducing ◽  
Sulfide Production ◽  
Reducing Bacteria

AbstractIntestinal sulfate-reducing bacteria are often isolated from patients with inflammatory bowel disease, including ulcerative colitis, and can be involved in the development of gut inflammation. A comparison of the metabolism of intestinal sulfate-reducing bacteria isolated from individuals with colitis and healthy controls using statistical analysis has never been studied and described before. The aim of our research was to evaluate the parameters of dissimilatory sulfate reduction inDesulfovibriospecies that were isolated from the feces of healthy objects and individuals with colitis. Principal component analysis indicates that the strains that were isolated from individuals with colitis grouped in the same cluster by biomass accumulation and sulfide production, same as the strains isolated from healthy individuals. Sulfate and lactate consumption measured over time showed negative correlation (Pearson correlations,p<0.01), healthy: -0.760; colitis: -0.770; healthy: -0.828; colitis: -0.847, respectively. The calculated linear regression (R2) was lower in biomass accumulation and hydrogen sulfide production, 0.531; 0.625 respectively. Thus, biomass accumulation and sulfide production, together with measured kinetic parameters play an important factor in bowel inflammation, including ulcerative colitis. Additionally, acetate production can also synergize with H2S, while sulfate consumption and lactate oxidation likely represent minor factors in bowel disease.

scholarly journals Evaluation of Physiological Parameters of Intestinal Sulfate-Reducing Bacteria Isolated from Patients Suffering from IBD and Healthy People

2020 ◽  
Vol 9 (6) ◽  
pp. 1920 ◽  
Author(s):  
Ivan Kushkevych ◽  
Jorge Castro Sangrador ◽  
Dani Dordević ◽  
Monika Rozehnalová ◽  
Martin Černý ◽  
...  
Keyword(s):  
Sulfate Reduction ◽  
Biomass Accumulation ◽  
Sulfate Reducing Bacteria ◽  
Dissimilatory Sulfate Reduction ◽  
Healthy Individuals ◽  
Fecal Samples ◽  
Sulfate Reducing ◽  
Cell Extracts ◽  
Production Of Hydrogen ◽  
Reducing Bacteria

Background: Inflammatory bowel diseases (IBDs) are multifactorial illnesses of the intestine, to which microorganisms are contributing. Among the contributing microorganisms, sulfate-reducing bacteria (SRB) are suggested to be involved in the process of bowel inflammation due to the production of hydrogen sulfide (H2S) by dissimilatory sulfate reduction. The aims of our research were to physiologically examine SRB in fecal samples of patients with IBD and a control group, their identification, the study of the process of dissimilatory sulfate reduction (sulfate consumption and H2S production) and biomass accumulation. Determination of biogenic elements of the SRB and evaluation of obtained parameters by using statistical methods were also included in the research. The material for the research consisted of 14 fecal samples, which was obtained from patients and control subjects. Methods: Microscopic techniques, microbiological, biochemical, biophysical methods and statistical analysis were included. Results: Colonies of SRB were isolated from all the fecal samples, and subsequently, 35 strains were obtained. Vibrio-shaped cells stained Gram-negative were dominant in all purified studied strains. All strains had a high percentage of similarity by the 16S rRNA gene with deposited sequences in GenBank of Desulfovibrio vulgaris. Cluster analysis of sulfate reduction parameters allowed the grouping of SRB strains. Significant (p < 0.05) differences were not observed between healthy individuals and patients with IBD with regard to sulfate reduction parameters (sulfate consumption, H2S and biomass accumulation). Moreover, we found that manganese and iron contents in the cell extracts are higher among healthy individuals in comparison to unhealthy individuals that have an intestinal bowel disease, especially ulcerative colitis. Conclusions: The observations obtained from studying SRB emphasize differences in the intestinal microbial processes of healthy and unhealthy people.

scholarly journals Catalase Activity of Sulfate-Reducing Bacteria Desulfovibrio piger Vib-7 and Desulfomicrobium sp. Rod-9 Isolated from Human Large Intestine

2014 ◽  
Vol 3 (1) ◽  
pp. 15-20 ◽  
Author(s):  
Ivan V Kushkevych ◽  
Roman V Fafula ◽  
Halyana L Antonyak
Keyword(s):  
Large Intestine ◽  
Catalase Activity ◽  
Sulfate Reducing Bacteria ◽  
Kinetic Properties ◽  
Bacterial Strains ◽  
Sulfate Reducing ◽  
Michaelis Constants ◽  
Human Large Intestine ◽  
Reducing Bacteria ◽  
Substrate Concentrations

Catalase activity of the sulfate-reducing bacteria Desulfovibrio piger Vib-7 and Desulfomicrobium sp. Rod-9 isolated from the human large intestine was studied. The high activity of the enzyme in cell-free extracts of both bacterial strains was determined (1745.21±154.67 and 873.11±72.23 U×mg-1 protein for D. piger Vib-7 and Desulfomicrobium sp. Rod-9, respectively). The effect of different temperature and pH as well as H2O2 concentration and time of incubation on the catalase activity in the cell-free extracts of D. piger Vib-7 and Desulfomicrobium sp. Rod-9 were examined. The maximum catalase activity for both bacterial strains at +30ºC temperature was determined. The highest activity of the studied enzyme in the cell-free extracts of D. piger Vib-7 at pH 7.5 and Desulfomicrobium sp. Rod-9 at pH 7.0 was measured. Based on experimental data, the analysis of the kinetic properties of the catalase by the studied bacteria was carried out. Increasing of hydrogen peroxide concentrations from 0.5 to 5.0 mM causes a monotonic rise of studied enzyme activity and the activity was maintained on an unchanged level (plateau) under substrate concentrations over 5.0 mM. The catalase activity, initial (instantaneous) reaction rate (V0) and maximum rate of the catalase reaction (Vmax) were significantly higher in D. piger Vib-7 than in Desulfomicrobium sp. Rod-9 cells. Michaelis constants (Km) of the catalase reaction were 8.01±0.77 and 10.33±0.98 mM for D. piger Vib-7 and Desulfomicrobium sp. Rod-9, respectively. DOI: http://dx.doi.org/10.3329/mh.v3i1.19776 Microbes and Health, June 2014. 3(1): 15-20

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