scholarly journals Evaluation of Physiological Parameters of Intestinal Sulfate-Reducing Bacteria Isolated from Patients Suffering from IBD and Healthy People

2020 ◽  
Vol 9 (6) ◽  
pp. 1920 ◽  
Author(s):  
Ivan Kushkevych ◽  
Jorge Castro Sangrador ◽  
Dani Dordević ◽  
Monika Rozehnalová ◽  
Martin Černý ◽  
...  
Keyword(s):  
Sulfate Reduction ◽  
Biomass Accumulation ◽  
Sulfate Reducing Bacteria ◽  
Dissimilatory Sulfate Reduction ◽  
Healthy Individuals ◽  
Fecal Samples ◽  
Sulfate Reducing ◽  
Cell Extracts ◽  
Production Of Hydrogen ◽  
Reducing Bacteria

Background: Inflammatory bowel diseases (IBDs) are multifactorial illnesses of the intestine, to which microorganisms are contributing. Among the contributing microorganisms, sulfate-reducing bacteria (SRB) are suggested to be involved in the process of bowel inflammation due to the production of hydrogen sulfide (H2S) by dissimilatory sulfate reduction. The aims of our research were to physiologically examine SRB in fecal samples of patients with IBD and a control group, their identification, the study of the process of dissimilatory sulfate reduction (sulfate consumption and H2S production) and biomass accumulation. Determination of biogenic elements of the SRB and evaluation of obtained parameters by using statistical methods were also included in the research. The material for the research consisted of 14 fecal samples, which was obtained from patients and control subjects. Methods: Microscopic techniques, microbiological, biochemical, biophysical methods and statistical analysis were included. Results: Colonies of SRB were isolated from all the fecal samples, and subsequently, 35 strains were obtained. Vibrio-shaped cells stained Gram-negative were dominant in all purified studied strains. All strains had a high percentage of similarity by the 16S rRNA gene with deposited sequences in GenBank of Desulfovibrio vulgaris. Cluster analysis of sulfate reduction parameters allowed the grouping of SRB strains. Significant (p < 0.05) differences were not observed between healthy individuals and patients with IBD with regard to sulfate reduction parameters (sulfate consumption, H2S and biomass accumulation). Moreover, we found that manganese and iron contents in the cell extracts are higher among healthy individuals in comparison to unhealthy individuals that have an intestinal bowel disease, especially ulcerative colitis. Conclusions: The observations obtained from studying SRB emphasize differences in the intestinal microbial processes of healthy and unhealthy people.

scholarly journals Analysis of pH dose-dependent growth of sulfate-reducing bacteria

Open Medicine ◽  
2019 ◽  
Vol 14 (1) ◽  
pp. 66-74 ◽  
Author(s):  
Ivan Kushkevych ◽  
Dani Dordević ◽  
Monika Vítězová
Keyword(s):  
Ulcerative Colitis ◽  
Hydrogen Sulfide ◽  
Sulfate Reduction ◽  
Sulfate Reducing Bacteria ◽  
Alkaline Media ◽  
Dissimilatory Sulfate Reduction ◽  
Lactate Oxidation ◽  
Sulfate Reducing ◽  
Inflammatory Bowel ◽  
Reducing Bacteria

AbstractLower intraluminal colonic pH is an indication for the development of inflammatory bowel disease including active ulcerative colitis. Involvement of intestinal sulfate-reducing bacteria in decreasing bowel pH by the production of H2S and acetate as well as their sensitivity has never been reported before. The study of the relative pH and survival of Desulfovibrio piger Vib-7 by monitoring sulfate reduction parameters was the aim of this work. Monitoring was done through the measurement of bacterial growth (biomass), dissimilatory sulfate reduction parameters: sulfate consumption, lactate oxidation, hydrogen sulfide and acetate production. According to our results, we observed that lower pH (<5) significantly inhibited D. piger Vib-7 growth. This inhibition was also noticed when alkaline media (>9 pH) was used, though the reduction was not at the rate as in media with pH of 4. The research indicates that the growth of D. piger Vib-7 is inhibited at pH of 4 which is not as low as the pH found in people with severely developed inflammatory bowel diseases such as ulcerative colitis. Certainly the interaction (synergistic effect) between both hydrogen sulfide and acetate accumulation can also play an important etiological role in the development of bowel inflammation in humans and animals.

scholarly journals Analysis of physiological parameters of Desulfovibrio strains from individuals with colitis

2019 ◽  
Vol 13 (1) ◽  
pp. 481-488 ◽  
Author(s):  
Ivan Kushkevych ◽  
Dani Dordević ◽  
Peter Kollár
Keyword(s):  
Ulcerative Colitis ◽  
Bowel Disease ◽  
Principal Component ◽  
Biomass Accumulation ◽  
Sulfate Reducing Bacteria ◽  
Dissimilatory Sulfate Reduction ◽  
Lactate Oxidation ◽  
Sulfate Reducing ◽  
Sulfide Production ◽  
Reducing Bacteria

AbstractIntestinal sulfate-reducing bacteria are often isolated from patients with inflammatory bowel disease, including ulcerative colitis, and can be involved in the development of gut inflammation. A comparison of the metabolism of intestinal sulfate-reducing bacteria isolated from individuals with colitis and healthy controls using statistical analysis has never been studied and described before. The aim of our research was to evaluate the parameters of dissimilatory sulfate reduction inDesulfovibriospecies that were isolated from the feces of healthy objects and individuals with colitis. Principal component analysis indicates that the strains that were isolated from individuals with colitis grouped in the same cluster by biomass accumulation and sulfide production, same as the strains isolated from healthy individuals. Sulfate and lactate consumption measured over time showed negative correlation (Pearson correlations,p<0.01), healthy: -0.760; colitis: -0.770; healthy: -0.828; colitis: -0.847, respectively. The calculated linear regression (R2) was lower in biomass accumulation and hydrogen sulfide production, 0.531; 0.625 respectively. Thus, biomass accumulation and sulfide production, together with measured kinetic parameters play an important factor in bowel inflammation, including ulcerative colitis. Additionally, acetate production can also synergize with H2S, while sulfate consumption and lactate oxidation likely represent minor factors in bowel disease.

scholarly journals Dissimilatory sulfate reduction in bacteria Desulfovibrio desulfuricans ІМV К-6 upon influence of Uragan and Raundup herbicides

2015 ◽  
Vol 6 (1) ◽  
pp. 40-44
Author(s):  
G. І. Zvir ◽  
O. М. Moroz ◽  
S. O. Hnatush
Keyword(s):  
Hydrogen Sulfide ◽  
Sulfate Reduction ◽  
Colorimetric Method ◽  
Reduction Process ◽  
Desulfovibrio Desulfuricans ◽  
Sulfate Reducing Bacteria ◽  
Dissimilatory Sulfate Reduction ◽  
Sulfate Ions ◽  
Sulfate Reducing ◽  
Reducing Bacteria

Objects of the study were sulfate-reducing bacteria Desulfovibrio desulfuricans ІМV К-6, isolated from Yavorivske lakе. This strain is kept in the collection of microorganisms at the Department of Microbiology of Ivan Franko National University. Bacteria were grown in the Kravtsov-Sorokin’s liquid medium with the following composition (g/l): Na2SO4 × 10H2O – 0.5, NaH2PO4 – 0.3, K2HPO4 – 0.5, (NH4)2SO4 – 0.2, MgSO4 × 7H2O – 0.1, C3H5O3Na – 2.0. The bacteria were grown for 10 days at 30 °C under anaerobic conditions. In order to study the sensitivity of the sulfate reducing bacteria to action of Uragan and Raundup herbicides, the cells of D. desulfuricans ІМV К-6 were grown at the concentrations of herbicides as follows: 0,28 mМ, 2,8 mМ (concentration recommended for use) and 5,6 mM. Biomass was determined by photometric method. Concentration of hydrogen sulfide in the culture medium was determined by photo-colorimetric method. Concentration of sulfate-ions in the medium was determined by turbidimetric method. Capacity of sulfate reducing bacteria D. desulfuricans ІМV K-6 to grow, reducing sulfates to hydrogen sulfide upon influence of Uragan and Raundup herbicides was studied. Accumulation of bacterial biomass in the control and upon influence of herbicides was the highest on the fourth-sixth day of cultivation, and after that the stationary growth phase began. It was shown that sulfate reducing bacteria upon influence of herbicides grew more intensively compared with the control. It was discovered that the level of biomass changed depending on the increasing concentration of Uragan or Raundup herbicides in the medium. Sulfate reducing bacteria D. desulfuricans ІМV K-6 could reduce sulfates to hydrogen sulfide in the presence of sulfates and organic compounds in the medium (dissimilatory sulfate reduction). Stimulatory influence of Uragan and Raundup on the dissimilatory sulfate reduction process of D. desulfuricans ІМВ К-6 has been discovered. The formation of hydrogen sulfide correlates with the usage of sulfatе ions. The capacity of sulfate reducing bacteria D. desulfuricans ІМV K-6 to grow, reducing sulfate ions to hydrogen sulfide upon influence of Uragan and Raundup may be caused by presence of inert components (sulfates) in these herbicides that can be used by microorganisms as electron acceptors during sulfate respiration. 

Microbial ecology of sulfate-reducing bacteria in wastewater biofilms analyzed by microelectrodes and fish (fluorescent in situ hybridization) technique

1999 ◽  
Vol 39 (7) ◽  
pp. 41-47 ◽  
Author(s):  
Satoshi Okabe ◽  
Hisashi Satoh ◽  
Tsukasa Itoh ◽  
Yoshimasa Watanabe
Keyword(s):  
In Situ Hybridization ◽  
Sulfate Reduction ◽  
Sulfate Reducing Bacteria ◽  
Hybridization Technique ◽  
Concentration Profiles ◽  
Reduction Zone ◽  
Sulfate Reducing ◽  
Reducing Bacteria ◽  
Culture Dependent

The vertical distribution of sulfate-reducing bacteria (SRB) in microaerophilic wastewater biofilms grown on fully submerged rotating disk reactors (RDR) was determined by the conventional culture-dependent MPN method and in situ hybridization of fluorescently-labelled 16S rRNA-targeted oligonucleotide probes for SRB in parallel. Chemical concentration profiles within the biofilm were also measured using microelectrodes for O2, S2-, NO3- and pH. In situ hybridization revealed that the SRB probe-stained cells were distributed throughout the biofilm even in the oxic surface zone in all states from single scattered cells to clustered cells. The higher fluorescence intensity and abundance of SRB probe-stained cells were found in the middle part of the biofilm. This result corresponded well with O2 and H2S concentration profiles measured by microelectrodes, showing sulfate reduction was restricted to a narrow anaerobic zone located about 500 μm below the biofilm surface. Results of the MPN and potential sulfate reducing activity (culture-dependent approaches) indicated a similar distribution of cultivable SRB in the biofilm. The majority of the general SRB probe-stained cells were hybridized with SRB 660 probe, suggesting that one important member of the SRB in the wastewater biofilm could be the genus Desulfobulbus. An addition of nitrate forced the sulfate reduction zone deeper in the biofilm and reduced the specific sulfate reduction rate as well. The sulfate reduction zone was consequently separated from O2 and NO3- respiration zones. Anaerobic H2S oxidation with NO3- was also induced by addition of nitrate to the medium.

Interactions between filamentous sulfur bacteria, sulfate reducing bacteria and poly-P accumulating bacteria in anaerobic-oxic activated sludge from a municipal plant

1998 ◽  
Vol 37 (4-5) ◽  
pp. 599-603 ◽  
Author(s):  
Ryoko Yamamoto-Ikemoto ◽  
Saburo Matsui ◽  
Tomoaki Komori ◽  
Edja. Kofi. Bosque-Hamilton
Keyword(s):  
Activated Sludge ◽  
Sulfate Reduction ◽  
Reduction Rate ◽  
Sulfate Reducing Bacteria ◽  
Sulfate Reduction Rate ◽  
Filamentous Bulking ◽  
Sulfate Reducing ◽  
Sulfur Bacteria ◽  
Sulfate Reduction Rates ◽  
Reducing Bacteria

The interactions between filamentous sulfur bacteria (FSB), sulfate reducing bacteria (SRB) and poly-P accumulating bacteria (PAB) in the activated sludge of a municipal plant operated under anaerobic-oxic conditions were examined in batch experiments using return sludge (RAS) and settled sewage. Phosphate release and sulfate reduction occurred simultaneously under anaerobic conditions. SRB were more sensitive to temperature changes than PAB. SRB played an important role in the decomposition of propionate to acetate. When the sulfate reduction rates were high, there was a tendency for the maximum release of phosphate also to be high. This was explained by the fact that PAB utilized the acetate produced by SRB. Sulfur oxidizing bacteria were sensitive to temperature change. When the sulfate reduction rate was high, the sulfide oxidizing rate was also high and filamentous bulking occurred. The results showed that sulfate reduction was a cause of filamentous bulking due to Type 021N that could utilize reduced sulfur.

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